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Citations for Thermo Fisher :
601 - 650 of 10000+ citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... and 10% fetal bovine serum (GIBCO, USA). CHO cells were grown in DMEM/F12 containing 15 mM HEPES and 10% fetal bovine serum ...
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bioRxiv - Neuroscience 2024Quote: ... with RNA quantity and quality measured using a NanoDrop 2000 (Thermo Fisher Scientific, Waltham, MA). Random-primer first-strand cDNA synthesis was performed using 1ug of total RNA and a High Capacity cDNA Reverse Transcription Kit (Applied Biosystems ...
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bioRxiv - Neuroscience 2024Quote: ... Real-time qPCR reactions were set up in triplicates with the Applied Biosystems Applied Biosystems SYBR green (Applied Biosystems; Cat#A25778) or TaqMan® Fast Advanced Master Mix (Applied Biosystems ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were washed x3 with TBS-T for 5 minutes and treated with goat anti-mouse IgG (H+L) secondary antibody HRP (1:10000; 31430; Invitrogen, Waltham, MA) or goat anti-rabbit IgG (H+L ...
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bioRxiv - Neuroscience 2024Quote: ... A wick made of Kimwipe (#06-666; Fisher Scientific) was placed partially inside a capillary tube (#1B120F-4 ...
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bioRxiv - Neuroscience 2024Quote: ... Assays were performed on a Bio-Rad (Hercules, CA) CFX384 Real Time System using TaqMan (Thermo Fisher Scientific, Waltham, MA) primer/probe sets specific for mouse beta-actin ...
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bioRxiv - Neuroscience 2024Quote: ... Sugars were purchased from Fisher Scientific (Hampton, New Hampshire, USA): sucrose (#FS S5-500) ...
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bioRxiv - Neuroscience 2024Quote: ... Phospho-Tau (Thr181) (catalog no. MN1050; ThermoFisher); Phospho-Tau (Ser202 ...
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bioRxiv - Neuroscience 2024Quote: ... on a 7500 Fast Real-time PCR system (Applied Biosystems). Expression levels were normalized to Hprt levels using the 2–ΔCt method ...
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Arc mediates intercellular synaptic plasticity via IRSp53-dependent extracellular vesicle biogenesisbioRxiv - Neuroscience 2024Quote: ... 15 mM HEPES (Thermo Fisher), 1 mM Sodium Pyruvate (Thermo Fisher) ...
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bioRxiv - Neuroscience 2024Quote: ... cDNA was synthesized from resulting RNA using cDNA VILO kit (Invitrogen, Cat#11754050). Relative gene expression was determined using gene-specific primers (PrimerBank ...
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bioRxiv - Neuroscience 2024Quote: ... The levels of soluble and insoluble human Aβ40 and Aβ42 in these extracts were quantified using ELISA kits (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... Phospho-Tau (Ser202, Thr205) (catalog no. MN1020; ThermoFisher); Tau antibody (catalog no ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were then stained for 30 minutes with antibodies against mouse CD45-ef780 (Thermo Fisher scientific ...
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Arc mediates intercellular synaptic plasticity via IRSp53-dependent extracellular vesicle biogenesisbioRxiv - Neuroscience 2024Quote: ... neurons were washed with PBS and mounted in Fluoromount G Mounting Medium with or without DAPI (Thermo Fisher) for ICC.
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bioRxiv - Neuroscience 2024Quote: ... with initial quantitation conducted using a NanoDrop ND-1000 spectrophotometer (Thermo Fisher Scientific). The quality of the RNA was inspected using a 2100 Bioanalyzer (Agilent Technologies) ...
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bioRxiv - Neuroscience 2024Quote: ... Tau antibody (catalog no. MN1000; ThermoFisher); Phospho-NF-κB p65 (rabbit ...
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bioRxiv - Neuroscience 2024Quote: ... Antibodies used were: Phospho-Tau (Thr231) (catalog no. MN1040; ThermoFisher); Phospho-Tau (Thr181 ...
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Arc mediates intercellular synaptic plasticity via IRSp53-dependent extracellular vesicle biogenesisbioRxiv - Neuroscience 2024Quote: ... at 37°C in pH 7.2 Minimum Essential Media (Thermo Fisher) supplemented with 2% GlutaMAX ...
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Arc mediates intercellular synaptic plasticity via IRSp53-dependent extracellular vesicle biogenesisbioRxiv - Neuroscience 2024Quote: ... the neurons were given 12 hours in growth media at 37°C/5% CO2 to allow sufficient recovery and expression of the plasmid prior to fixation in 4% formaldehyde (Thermo Fisher) / 4% sucrose (VWR ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were split and the following day dqBSA (Invitrogen, catalog no. D12051) was added in HBSS with or without 10 µM UA or bafilomycin A1 (Tocris Bioscience) ...
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Arc mediates intercellular synaptic plasticity via IRSp53-dependent extracellular vesicle biogenesisbioRxiv - Neuroscience 2024Quote: ... 1% penicillin and streptomycin (Thermo Fisher) on coverslips (No ...
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Arc mediates intercellular synaptic plasticity via IRSp53-dependent extracellular vesicle biogenesisbioRxiv - Neuroscience 2024Quote: Neurons were transfected after DIV14-15 using lipofectamine 2000 (Thermo Fisher) at a 3:1 ratio when complexed with plasmid DNA ...
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bioRxiv - Neuroscience 2024Quote: ... containing 10% fetal bovine serum and 1% GlutaMAX (Thermo Fisher Sceintific) with or without 10 µM UA for 6 days ...
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bioRxiv - Neuroscience 2024Quote: ... HMC3 were cultured in Eagle’s Minimum Essential Medium (Gibco) with 10% fetal bovine serum in a humidified incubator with 5% CO2 at 37°C ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were then stained for 30min in anti CD45 conjugated APC-Cy7 antibody (Thermo Fisher scientific ...
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bioRxiv - Neuroscience 2024Quote: ... Human IL-1β was measured by IL-1β kit (Thermo Fisher, catalog no. 88-7261). Human Aβ42 was measured by Aβ42 kit (ExCell Bio ...
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bioRxiv - Neuroscience 2024Quote: ... and qPCR analysis was done with power SYBR Green PCR master mix (Thermo Fisher, catalog no. A46109). The primers used to amplify each transcript were as following ...
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bioRxiv - Neuroscience 2024Quote: ... supplemented with 10% fetal bovine serum (FBS, Gibco, #30084) and cultured in a humidified atmosphere with 5% CO2 at 37 °C ...
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bioRxiv - Pathology 2024Quote: ... SYTOXTM-Blue (Invitrogen, S34857) was added to cell suspension to exclude dead cells and sorting was carried out using FACSAria III cell sorter (BD Biosciences) ...
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bioRxiv - Pathology 2024Quote: Raw MS/MS data files were analyzed using the default parameters of Proteome Discoverer 2.2 (Thermo Scientific). Mascot search engine (Matrix Science ...
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bioRxiv - Neuroscience 2024Quote: ... Relative gene expression was determined using gene-specific primers (PrimerBank) and SYBR Green master mix (Life Technologies) on a 7500 Fast Real-time PCR system (Applied Biosystems) ...
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bioRxiv - Neuroscience 2024Quote: ... CD11b-FITC (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... Slides were then washed 3x with PBS and mounted with Prolong antifade DAPI medium (Invitrogen, #P36935). Confocal images were taken on a Leica SP8 microscope with a 63x oil objective ...
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bioRxiv - Neuroscience 2024Quote: ... Total RNA was purified with a PureLink™ RNA Mini Kit (Thermo Fisher Scientific, catalog no. 12183018A) as per the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... were maintained in high-glucose DMEM (Gibco) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2024Quote: ... qPCR was conducted in triplicate using AceQ SYBR qPCR Master Mix (ThermoFisher) in the Light-Cycler 480 detection System (Roche ...
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bioRxiv - Neuroscience 2024Quote: ... and 1% Penicillin-Streptomycin (Gibco). The cell suspension was collected by a 70 µM nylon cell filter and centrifuged at 1000 rpm for 5 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... supplemented with 2% B-27 (Gibco), 1.8% HEPES ...
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bioRxiv - Pathology 2024Quote: ... ZO-1 (#61-7300, Invitrogen, 1/100) and CLDN5 (#PAB27037 ...
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bioRxiv - Pathology 2024Quote: ... Nuclei were labeled with Hoechst for 10 minutes and mounting medium was added on the thrombi section after extensive washes in PBS +/+ and secured with a lamella (ProLong Gold Antifade, Thermofisher Scientific). Thrombi sections were visualized with the EVOS cell imaging system (Thermo Fisher Scientific ...
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bioRxiv - Pathology 2024Quote: ... Thrombi sections were visualized with the EVOS cell imaging system (Thermo Fisher Scientific) minimum 24 hours after the assembling of the slide and lamella ...
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bioRxiv - Pathology 2024Quote: ... Neutrophils and NETs were visualized under a fluorescence microscope (EVOS cell imaging system, Thermo Fisher Scientific).
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bioRxiv - Pathology 2024Quote: ... ZO-1 (#61-7300, Invitrogen, 1/100) and CLDN5 (#PAB27037 ...
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bioRxiv - Pathology 2024Quote: ... primary dermal fibroblasts (normal, human, adult; PCS-201-012, ATCC, VA, USA) were cultured in Dulbecco’s Modified Eagle medium (DMEM) (Thermo Fisher Scientific Inc., Waltham, MA, USA) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Pathology 2024Quote: ... and total RNA was then extracted using PureLink RNA Mini Kits (Thermo Fisher Scientific). The amount of RNA was quantified using a NanoDrop 1000 spectrophotometer (ND-1000 ...
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bioRxiv - Pathology 2024Quote: ... TRIzol reagent (Invitrogen, Carlsbad, CA, USA) was added ...
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bioRxiv - Pathology 2024Quote: Total protein was extracted from cultured cells using Pierce RIPA Buffer (Thermo Fisher Scientific) containing 1% Halt Protease Inhibitor Cocktail (Thermo Fisher Scientific) ...
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bioRxiv - Pathology 2024Quote: ... The amount of RNA was quantified using a NanoDrop 1000 spectrophotometer (ND-1000; Thermo Scientific, Rockford, IL, USA). Then to synthesize cDNA ...
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bioRxiv - Pathology 2024Quote: ... The total protein concentration was quantified by BCA assay (23227, Thermo Fisher Scientific). SDS[PAGE was used to separate the proteins ...