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Citations for Thermo Fisher :
601 - 650 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... and chromatin stained with DAPI (1μg/mL, D1306, Thermo Scientific). Primary antibody was omitted as a negative control ...
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bioRxiv - Neuroscience 2024Quote: ... on culture plates pre-coated with Geltrex (Thermo Fisher Scientific) diluted in DMEM (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... Phagocytic function was demonstrated using 1 um yellow-green FluoSpheres (Thermo Fisher Scientific) and antigen presentation function was demonstrated by successful activation of T-cells following 24-hour moDC pre-exposure to the hyper-immunogenic hemocyanin from Megathura crenulata (keyhole limpet hemocyanin ...
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bioRxiv - Neuroscience 2024Quote: ... Standard culture conditions involved the use of Corning tissue culture plates (Thermo Fisher Scientific) and incubation at 37°C/5% CO2.
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bioRxiv - Neuroscience 2024Quote: ... First-strand cDNA was then transcribed using the SuperScript IV Reverse Transcriptase (Invitrogen, ThermoFisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... All single cell dissociations were done by incubating with StemPro Accutase (Gibco) for 4-6 minutes at 37°C ...
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bioRxiv - Neuroscience 2024Quote: ... Quantitative real-time PCR was performed in an ABI Prism 7300 using the Fast SYBR Green Master mix (Applied Biosystems, Waltham, MA, USA) and primers (detailed below ...
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bioRxiv - Neuroscience 2024Quote: ... and anti-GluN2A (1:1,000; 480031) was from ThermoFisher. Mouse anti–β-tubulin (1:2,000 ...
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bioRxiv - Neuroscience 2024Quote: ... 1% P/S (Gibco, Cat#: 15140-122)) or DMSO as a control ...
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bioRxiv - Neuroscience 2024Quote: ... into HEK293T cells grown in 15 cm Petri dishes to ∼80% confluence in DMEM (GIBCO) supplemented with 10% HyClone cosmic calf serum ...
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bioRxiv - Neuroscience 2024Quote: ... were transfected using Lipofectamine 3000 (ThermoFisher) into HEK293T cells grown in 15 cm Petri dishes to ∼80% confluence in DMEM (GIBCO ...
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bioRxiv - Neuroscience 2024Quote: ... and 50 µg/ml gentamycin (GIBCO) without growth factors ...
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bioRxiv - Neuroscience 2024Quote: ... and 50 µg/ml gentamycin (GIBCO).
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bioRxiv - Neuroscience 2024Quote: ... 2% (v/v) B27 neural supplement (GIBCO) and 50 µg/ml gentamycin (GIBCO ...
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bioRxiv - Neuroscience 2024Quote: ... respectively as well as the staining dye Hoechst (1:200 dilution; ThermoFisher Scientific #62249). The optimal concentration for the uncoupler FCCP yielding maximal uncoupled respiration was previously determined based on a titration performed on HMC3 microglia (see Supplemental Figure 4).
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bioRxiv - Neuroscience 2024Quote: ... the cells were plated into 35 mm glass-bottom dishes with DMEM/F12 differentiation media (GIBCO) supplemented with 2 μg/ml heparin (ScienceCell) ...
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bioRxiv - Neuroscience 2024Quote: ... were grown in DMEM/F12 media (GIBCO) supplemented with HyClone cosmic calf serum (GE Healthcare ...
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bioRxiv - Microbiology 2024Quote: ... 2.5 µl of the sample was applied to a freshly glow-discharged Quantifoil R 3.5/1 Cu/Rh 200 mesh grid and plunge-frozen into liquid ethane using a Vitrobot Mark IV (ThermoFisher) at 100% humidity at an ambient temperature of 10 °C ...
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bioRxiv - Microbiology 2024Quote: Single particle cryo-EM data was collected on a Titan Krios G2 microscope (ThermoFisher) operating at an acceleration voltage of 300 kV ...
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bioRxiv - Microbiology 2024Quote: ... The supernatants from the second centrifugation step were then dialysed overnight at 4 °C against ice-cold buffer B (50 mM Tris pH 7.5, 150 mM NaCl) in SnakeSkin dialysis tubing (Molecular weight cutoff 3 kDa, Thermo Fisher Scientific) until the sample reached pH 7.5 ...
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bioRxiv - Neuroscience 2024Quote: ... Slides were mounted with prolong glass mounting media (Invitrogen, P36984) containing DAPI ...
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bioRxiv - Neuroscience 2024Quote: ... Neurons were cultured and maintained in Neurobasal medium (21103049; Gibco), supplemented with B27 (17504044 ...
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bioRxiv - Neuroscience 2024Quote: 0.5-1 ug of RNA was reverse transcribed to cDNA using the High-Capacity cDNA Reverse Transcription Kit (Invitrogen). Following this ...
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bioRxiv - Neuroscience 2024Quote: ... induction media was removed and replaced with neuronal maintenance media: Neurobasal (Gibco), supplemented with 1x B27 (Gibco) ...
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bioRxiv - Neuroscience 2024Quote: ... qPCR analysis was performed using SYBR Green Master Mix (ThermoFisher) for all qPCRs except C9orf72 variant 2 expression levels for which TaqMan probes were used (sequences provided in Table S2 ...
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bioRxiv - Neuroscience 2024Quote: ... fed with fresh E8 media (Gibco) daily ...
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bioRxiv - Neuroscience 2024Quote: ... Protein concentration was determined via BCA assay (Thermo Fisher) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... 1x HEPES (Gibco), 1x NEAA (Gibco) ...
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bioRxiv - Neuroscience 2024Quote: ... containing 1x N2 (Thermo Fisher Scientific), 1x Glutamine (Gibco) ...
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bioRxiv - Neuroscience 2024Quote: ... larvae were euthanised with an overdose of 2-phenoxyethanol (ACROS Organics). Experimental procedures were in accordance with the Animals (Scientific Procedures ...
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bioRxiv - Neuroscience 2024Quote: ... and transfection reagents (lipofectamine 2000 in conjunction with Opti-MEM) were purchased from Life Technologies (Waltham, MA). SAFC fetal bovine serum (FBS) ...
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bioRxiv - Neuroscience 2024Quote: ... were cut with razor blades and 2.0 mm-diameter medial prefrontal cortical tissue punches were collected on dry ice using Integra Miltex Biopsy Punchers with Plunger System (Fisher Scientific, Waltham, MA). The punches were placed in labeled ...
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bioRxiv - Neuroscience 2024Quote: ... and 2.5 µL of 100X Halt™ Protease and Phosphatase Inhibitor Cocktail (Thermo Fisher Scientific, Waltham, MA) were added ...
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bioRxiv - Neuroscience 2024Quote: ... anti rabbit Alexa Fluor 488 (IF secondary 1:600, Thermo Fisher, Z25302); anti mouse Alexa Fluor 568 (IF secondary 1:600 ...
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bioRxiv - Neuroscience 2024Quote: ... anti rabbit Alexa Fluor 568 (IF secondary 1:600, Thermo Fisher, Z25306).
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bioRxiv - Neuroscience 2024Quote: ... anti mouse Alexa Fluor 568 (IF secondary 1:600, Thermo Fisher, Z25006); anti rabbit Alexa Fluor 568 (IF secondary 1:600 ...
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bioRxiv - Neuroscience 2024Quote: ... Immunofluorescence or DAB was used for visualized (15454629, Thermo Fisher) using a Leica DMi1 light microscope.
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bioRxiv - Neuroscience 2024Quote: ... RT-PCR for splicing events was conducted using 2X PCR Master Mix (11816843, Thermo Fisher) with specific primers (see below ...
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bioRxiv - Neuroscience 2024Quote: RNA extractions were carried out using TRIzol (15596-026, Invitrogen), according to manufacturer instructions ...
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bioRxiv - Neuroscience 2024Quote: ... coli BL21DE3 strain (Life technologies). Then ...
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bioRxiv - Neuroscience 2024Quote: ... GFP (Thermo Fisher, #A10262), gephyrin (Synaptic Systems ...
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bioRxiv - Neuroscience 2024Quote: ... Anti-rabbit IgG-Alexa 488 (1:250, ThermoFisher #A21206), before mounting.
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bioRxiv - Neuroscience 2024Quote: ... 5×104 cells suspended in 1 μL OptiMEM (Gibco) were stereotactically injected to the right frontal lobe of adult mice using a Hamilton syringe with a 33 g needle ...
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bioRxiv - Neuroscience 2024Quote: We then measured the point spread function (PSF) of the light-targeting system using subdiffraction-sized fluorescent beads (TetraSpeck microspheres, radius 50 nm; T7279, Invitrogen) embedded in agarose gel (1.5 % ...
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bioRxiv - Neuroscience 2024Quote: ... Images were acquired using an EVOS FL fluorescent imaging microscope (Life Technologies or Leica).
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bioRxiv - Neuroscience 2024Quote: ... images were collected using TEM (Thermo Fisher Scientific) operated at 120 kV.
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bioRxiv - Neuroscience 2024Quote: ... Fluorescence single stains for compensation were performed using single stained cell samples and/or UltraComp eBeadsTM (Invitrogen). Cells were washed then run fresh or fixed in 0.25% PFA in PBS ...
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bioRxiv - Neuroscience 2024Quote: ... glutaMAX (35050061; Gibco), and antibiotics ...
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bioRxiv - Neuroscience 2024Quote: MACS-enriched Pan T-cells and PBMCs were stained with the eBioscience eFluor 450 Cell Proliferation Dye (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: All long-term cultures were performed in the presence of 100 U/ml penicillin-streptomycin (Thermo Fisher Scientific). Standard culture conditions involved the use of Corning tissue culture plates (Thermo Fisher Scientific ...