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Citations for Thermo Fisher :
651 - 700 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... cells were incubated with LysoTracker dye (Invitrogen, Thermofisher, #L7528) according to manufacturer’ instructions for 30’ ...
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bioRxiv - Cancer Biology 2024Quote: Total RNA was extracted from cell samples using TRIzol reagent (Invitrogen, #15596018) or the RNeasy Micro Kit (Quiagen) ...
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bioRxiv - Cancer Biology 2024Quote: ... was used at 2μL/mL diluted in Opti-MEM (Life Technologies, #31985062) to perform the transfection.
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bioRxiv - Cancer Biology 2024Quote: Cells were digested into single cells by trypsinization (0.25% trypsin-EDTA, Gibco, #25200056) and resuspended at a concentration of 106 cells/mL in a solution of 30% PBS and 70% ice-cold EtOH for fixation ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were stained with Annexin-V and Propidium Iodide apoptosis detection kit (Thermofisher, #88-8007-74) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... the media was replaced with DMEM + 10% FBS + CellEvent Caspase-3/7 Detection Reagent (Green) according to the manufacturers recommendations (Invitrogen). The plate was then imaged every 15 minutes using phase and GFP channels in the ZEISS Celldiscoverer 7 Automated Live Cell Imager set to 42°C at 5% CO2 and 20% O2 ...
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bioRxiv - Cancer Biology 2024Quote: ... Nat10-expressing cells were selected using Blasticidin (Gibco) at a concentration of 10 μg/ml.
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bioRxiv - Cancer Biology 2024Quote: Metaphase arrest of H2170 cells was performed by incubation with KaryoMAX (Gibco) at 0.1 ug mL-1 overnight ...
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bioRxiv - Cancer Biology 2024Quote: ... the NuPAGE protein gel was processed and stained using Pierce Silver Stain for Mass Spectrometry (Thermo Fisher). For tandem Mass Spectrometry (MS/MS ...
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bioRxiv - Cancer Biology 2024Quote: Sequencing-ready libraries were quantified using the Qubit dsDNA HS assay (Invitrogen) and quality controlled with the Bioanalyzer 2100 DNA HS assay (Agilent) ...
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bioRxiv - Cancer Biology 2024Quote: ... Total RNA extractions were quantified with a Nanodrop One (Thermo Fisher), and RNA integrity was assessed with the Bioanalyzer 2100 RNA Nano assay (Agilent) ...
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bioRxiv - Cancer Biology 2024Quote: ... Lipofectamine reagent RNAiMAX (Life Technologies, #13778075) was used at 2μL/mL diluted in Opti-MEM (Life Technologies ...
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bioRxiv - Cancer Biology 2024Quote: ... or Live/Dead Fixable Yellow dye (Invitrogen, #L34967), following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Maximum release was set up with 2% Triton X-100 (Acros Organics, Thermo Fisher Scientific, Waltham, MA, USA). All samples and controls were set up in triplicates ...
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bioRxiv - Cancer Biology 2024Quote: ... An alexaFluor-conjugated secondary antibody (1: 500, ThermoFisher Scientific) was diluted in a blocking solution and incubated for 1 h at 37°C ...
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bioRxiv - Cancer Biology 2024Quote: ... and RNase inhibitor (Thermo Fisher Scientific, #AM2694) for 30 min ...
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bioRxiv - Cancer Biology 2024Quote: ... Specific oligos against lnc-FANCI-2 or GAPDH listed in Table S5 were labeled with [γ-32P]-ATP using T4 PNK (Thermo Fisher Scientific, Waltham, MA, #18004–010) and added into hybridization buffer for overnight incubation at 42°C ...
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bioRxiv - Cancer Biology 2024Quote: ... maintained in Dulbecco’s modified Eagle medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2024Quote: ... the resin underwent trypsin digestion and peptides were run on an Orbitrap Fusion™ Lumos™ mass spectrometer (ThermoFisher Scientific) at the UNMC Proteomics Core and analyzed using Proteome Discoverer (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... 75 µm x 250 mm (Thermo Scientific) using a linear gradient of acetonitrile (3-36% ...
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bioRxiv - Cancer Biology 2024Quote: ... ΔYY1-D5 and ΔPr-A9 cells were extracted using TRIzol reagent (Thermo Fisher Scientific, Waltham, MA, #15596018). Total ribo-minus RNA-seq libraries ...
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bioRxiv - Cancer Biology 2024Quote: ... DAPI was used for nuclei counterstaining before mounting in a Prolong Gold Antifade mounting medium (Thermo Fisher Scientific, #P36934).
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bioRxiv - Cancer Biology 2024Quote: ... were purchased from the American Type Culture Collection (ATCC, Manassas, Virginia, United States) and maintained in RPMI complete media supplemented with 10% fetal bovine serum (FBS, Thermo Fisher Scientific, Waltham, MA, USA), 1% penicillin/streptomycin cocktail (Corning ...
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bioRxiv - Cancer Biology 2024Quote: TaqMan Gene Expression Assay (Applied Biosystems, #4331182) for GADPH (Hs02758991_g1 ...
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bioRxiv - Cancer Biology 2024Quote: ... using a Nano Easy-Spray Ion Source (Thermo Fisher Scientific) under the following settings ...
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bioRxiv - Cancer Biology 2024Quote: ... and 25mM HEPES (Thermo Fisher Scientific, Waltham, MA, USA).
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bioRxiv - Cancer Biology 2024Quote: ... 1.67 mM CaCl2 (Thermo Fisher Scientific) and 1 mM (NH4)2HPO4 (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2024Quote: ... CellTracker™ Green CMFDA Dye (Ex/Em 492/ 517 nm; Thermo Fisher, Waltham, MA, USA) was used to label the HS-5 cells ...
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bioRxiv - Cancer Biology 2024Quote: ... and the tumor pieces were washed with Hibernate A solution containing Glutamax (2 mM, ThermoFisher 35050061), Penicillin/Streptomycin (100 U/mL and 100 μg/mL ...
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bioRxiv - Cancer Biology 2024Quote: ... manually parcellated using a 1.0 mm biopsy punch (ThermoFisher 12460401), and suspended in 1 mL of Melanoma Organoid Complete Media (formula below ...
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bioRxiv - Cancer Biology 2024Quote: ... supplemented with protease and phosphatase inhibitor tablets (Life Technologies #A32961). Lysates were centrifuged at 8,000 g for 10 min and the supernatants were collected and stored at -80C ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA was extracted using the PureLink™ RNA Mini Kit (Invitrogen #12183018A) according to the manufacturer’s protocol (page 50 ...
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bioRxiv - Cancer Biology 2024Quote: ... PC3 were cultured in RPMI-1640 media + GlutaMax (Gibco, Thermo Fisher, Illkirch, France) supplemented with 5% fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2024Quote: ... containing protease and phosphatase inhibitors (Thermo Scientific, A32959) and then centrifuged at 13,000 rpm for 10 minutes at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... IP samples were resolved on a NuPAGE 10% Bis-Tris gel (Life Technologies, WG1201BX10) in 1x MOPS running buffer ...
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bioRxiv - Cancer Biology 2024Quote: Transient transfections were performed using Lipofectamine 2000 (ThermoFisher Scientific) at a ratio of nucleic acid:Lipofectamine 2000 of 1:2 for both DNA and siRNA ...
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bioRxiv - Cancer Biology 2024Quote: ... Sample concentrations were normalized using 6X SDS sample buffer (62.5 mM Tris pH 6.8, 5% SDS, 18% glycerol (Fisher Scientific G33-500), bromophenol blue (Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... C4-2 and C4-2b were cultured in RPMI-1640 media + GlutaMax (Gibco, Thermo Fisher, Illkirch, France) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2024Quote: ... The concentration of eluted RNA was determined using a NanoDrop™ One spectrophotometer (ThermoFisher Scientific). RT-qPCR was performed using the GoTaq® 1-Step RT-qPCR System (Promega ...
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bioRxiv - Cancer Biology 2024Quote: ... Nuclei were counterstained with DAPI and mounted in Prolong Gold (Invitrogen).
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bioRxiv - Cancer Biology 2024Quote: ... Protein lysates were incubated with either EGFR (Ab-13) mouse antibody (Thermo Fisher Scientific, MS- 609-P1) or Ub (P4D1 ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were maintained in serum-free medium (SFM; Gibco) supplemented with 25 µg/mL bovine pituitary extract (Gibco ...
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bioRxiv - Cancer Biology 2024Quote: The CRC cell lines (HT29, DLD1, HCT116, Lovo, SW48, SW480, and SW620) were cultured in McCoy media + GlutaMax (Gibco, Thermo Fisher, Illkirch, France) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2024Quote: ... the cells were incubated with Ca2+-free physiological saline solution (PSS) solution and treated with 2 µM Thapsigargin (TG) (Reference: T7458, Life Technologies, Thermo Fisher) to deplete intracellular store of Ca2+ ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA was extracted from the samples using TRIzol® Reagent (ThermoFisher Scientific) and analysed as described.
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bioRxiv - Cancer Biology 2024Quote: ... bromophenol blue (Fisher Scientific, BP115-25), 302 mM dithiothreitol (DTT ...
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bioRxiv - Cancer Biology 2024Quote: ... the cells were incubated with Ca2+-free physiological saline solution (PSS) solution and treated with 2 µM Thapsigargin (TG) (Reference: T7458, Life Technologies, Thermo Fisher) to deplete intracellular store of Ca2+ ...
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bioRxiv - Cancer Biology 2024Quote: ... C4-2 and C4-2b were cultured in RPMI-1640 media + GlutaMax (Gibco, Thermo Fisher, Illkirch, France) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2024Quote: ... Transfections were performed overnight in OptiMEM I Reduced Serum Media (ThermoFisher Scientific). Subsequent analyses were performed at 48 hours (DNA ...
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bioRxiv - Cancer Biology 2024Quote: ... and C33A (HPV− cervical squamous carcinoma) cells obtained from the ATCC were grown in DMEM supplemented with 10% FBS (ThermoFisher Scientific) and 50 U/mL penicillin/streptomycin (Lonza) ...