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Citations for Thermo Fisher :
501 - 550 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... SW1417 were cultured in RPMI-1640 medium (Thermo-Fisher Scientific#11875093), SNU283 ...
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bioRxiv - Cancer Biology 2024Quote: ... Real time qPCR was done using Fast SYBR Green Master Mix (Applied Biosystems #43-856-12) and ran on QuantStudio six Flex real-time PCR system (Applied Biosystems # 4485691) ...
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bioRxiv - Cancer Biology 2024Quote: ... and 1% sodium pyruvate (Gibco 11360-070). For knockdown of endogenous EWS::FLI in A673 ...
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bioRxiv - Cancer Biology 2024Quote: ... with 10% heat-inactivated fetal bovine serum (Gibco 16000-044), 1% penicillin/streptomycin/glutamine (P/S/Q ...
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bioRxiv - Cancer Biology 2024Quote: ... blots were developed using Pico- or Femto-chemiluminescent substrate (ThermoFisher) and visualized with autoradiography or digital imaging ...
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bioRxiv - Cancer Biology 2024Quote: ... 1% penicillin/streptomycin/glutamine (P/S/Q, Gibco 10378-016), and 0.3 mg/Ml Geneticin (Gibco 10131-027) ...
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bioRxiv - Cancer Biology 2024Quote: Whole-cell protein extraction was completed using Pierce™ RIPA buffer (ThermoFisher 88901) supplemented with Protease Inhibitor Cocktail (Sigma P8340-5ML ...
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bioRxiv - Cancer Biology 2024Quote: ... and ran on QuantStudio six Flex real-time PCR system (Applied Biosystems # 4485691). Individual gene expression levels were calculated using the change in cycling threshold (ΔCT ...
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bioRxiv - Cancer Biology 2024Quote: ... SRP68 (Invitrogen#100080), SRP54 (BD Bioscience #610940) ...
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bioRxiv - Cancer Biology 2024Quote: Protein concentration was determined using Pierce™ BCA® Protein Assay Kits from Thermo fisher (#23225). 10-40 mg of protein was used for western blots ...
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bioRxiv - Cancer Biology 2024Quote: ... SRP19 (Invitrogen #93197), ACTIN (Santa Cruz#A2017) ...
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bioRxiv - Cancer Biology 2024Quote: ... Click-iT Plus OPP Alexa Fluor 647 Protein Synthesis Assay kit (Invitrogen #C10458) was used as per manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Lipofectamine RNAiMAX (Invitrogen#13778150) was used for siRNA transfection according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2024Quote: ... and 0.3 mg/Ml Geneticin (Gibco 10131-027). A673 was obtained from American Type Culture Collection (ATCC ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were resuspended in a flow-cytometry buffer and DAPI (4′,6-diamidino-2-phenylindole, 0.5 μg/ml; ThermoFisher #D1306) solution to exclude dead cells ...
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bioRxiv - Cancer Biology 2024Quote: ... SNU61 were cultured in RPMI-1640 with 25mM HEPES (Thermo-Fisher Scientific#15630080).
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bioRxiv - Cancer Biology 2024Quote: ... SW1463 were cultured in DMEM with 1mM Na-Pyruvate (Thermo-Fisher Scientific#11360070), SNUC1 ...
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bioRxiv - Cancer Biology 2024Quote: ... 10 µg of each plasmid (gagpol, vsv-g, and transfer plasmid) were combined with 2 ml Opti-MEM I Reduced Serum Medium (Gibco 31985070) and 90 µl MirusBio TransIT-LT1 Transfection Reagent (Mirus MIR2306 ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were incubated for 2 hours with 10uM EdU and stained with LIVE/DEAD™ Fixable Violet Dead Cell Stain Kit (Invitrogen #L34955) before fixation ...
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bioRxiv - Cancer Biology 2024Quote: ... Lipofectamine RNAiMAX was diluted in Opti-MEM reduced serum media (ThermoFisher Scientific, 31985070) with 1.5 µL of Lipofectamine RNAiMAX for every 25 µL of Opti-MEM media ...
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bioRxiv - Cancer Biology 2024Quote: ... cDNA was detected using PowerUp SYBR Green Master Mix for qPCR (ThermoFisher Scientific, A25776). A master mix of 250 µM forward primer ...
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bioRxiv - Cancer Biology 2024Quote: ... 100 U/ml penicillin-streptomycin (Fisher Scientific, #11548876). All cell lines were tested negative for mycoplasma ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell cycle analysis was performed using the Click-iT Plus EdU Alexa Fluor 647 Flow Cytometry Assay Kit (Invitrogen #C10634). Cells were incubated for 2 hours with 10uM EdU and stained with LIVE/DEAD™ Fixable Violet Dead Cell Stain Kit (Invitrogen #L34955 ...
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bioRxiv - Cancer Biology 2024Quote: ... and 100 to 500 ng of RNA was reverse transcribed using the high-capacity cDNA reverse transcription kit (Thermofisher, #4368814). Real time qPCR was done using Fast SYBR Green Master Mix (Applied Biosystems #43-856-12 ...
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bioRxiv - Cancer Biology 2024Quote: ... CD45.2 BM cells were plated at 1×106 cells/ml in a 10 cm plate in 10 ml of complete StemPro-34 SFM (Thermo Fisher #10639011) containing recombinant mouse cytokines (20 ng/ml IL-3 ...
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bioRxiv - Cancer Biology 2024Quote: Total RNA was isolated from all cells using TRIzol reagent (Thermofisher, #15596026) and 100 to 500 ng of RNA was reverse transcribed using the high-capacity cDNA reverse transcription kit (Thermofisher ...
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bioRxiv - Cancer Biology 2024Quote: ... ER Tracker (E34250) were acquired from ThermoFisher and used as indicated in the manufacturers protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... Coverslips were mounted onto glass microscope slides with Anti-fade glass mountant (Thermo Fisher Scientific, 15898391). The cells were imaged with a Zeiss LSM 880 Fast Airyscan confocal microscope with 20X air and a 40X oil objectives ...
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bioRxiv - Cancer Biology 2024Quote: ... or Alexa Fluor 647 goat anti-rabbit (Invitrogen, A21245, 1:300 in 1% BSA/1X PBS). Cells were washed with 1X PBS (3 x 5 min ...
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bioRxiv - Cancer Biology 2024Quote: ... when indicated mitochondria were labeled by incubating live cells with MitoTracker Deep Red (Thermo Fisher Scientific, 12010156) in HBSS medium for 30 min at 37 °C then cells were washed once with 1X PBS ...
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bioRxiv - Cancer Biology 2024Quote: ... supplemented or not with 30 µg/mL of RNaseA and 75 U/mL of RNase T1 (RNase A/T1 mix, Thermo Fisher Scientific EN0551). Cells were fixed and permeabilized with ice cold MeOH for 10 min at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: Square coverslips (Thermo Fisher Scientific, 10474379) were heat-sterilized before cell seeding ...
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bioRxiv - Cancer Biology 2024Quote: ... 200 U/mL RNaseOUT (Invitrogen, 10777019), 2 mM phenylmethanesulfonyl fluoride ...
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bioRxiv - Cancer Biology 2024Quote: ... and 1X Mammary Epithelial Growth Supplement (MEGS, Invitrogen S-015-5). Mycoplasma contamination was frequently assessed by PCR ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were resuspended in 500 μL of FxCycle™PI/RNase Staining Solution (F10797, lot# 2561624, Invitrogen, Oregon) and left to incubate in the dark for 30 minutes.
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bioRxiv - Cancer Biology 2024Quote: ... and all libraries were quantified using Qubit 4.0 (Life Technologies). RNA-seq libraries were sequenced on Illumina NextSeq 550 (Illumina ...
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bioRxiv - Cancer Biology 2024Quote: ... 10% 2-mercaptoethanol (21985023; Life Technologies, Carlsbad, CA, USA), 30% glycerol ...
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bioRxiv - Cancer Biology 2024Quote: ... Quantification of proteins was carryout out with a BCA protein assay (23227; Thermo Fisher Scientific). Absorbance was measured at 562 nm using a SpectraMax i3x microplate reader (Molecular Devices LLC).
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bioRxiv - Cancer Biology 2024Quote: ... containing the guide RNA targeting SNX1 (GGACAACACGGCATTGTCA) or SNX2 (AAACGATTCGAAAAGAAGT) was transfected using Lipofectamine 2000 following the manufacturer’s instructions (Thermo Fisher Scientific). After 48 h ...
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bioRxiv - Cancer Biology 2024Quote: ... Lysates were clarified at 18,000 x g for 15 min and protein concentration was determined by the bicinchoninic acid method (BCA; Thermo Fisher Scientific). Protein samples were separated on ammediol SDS-PAGE [30] and transferred to a PVDF membrane (polyvinylidene difluoride ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA quality was checked through agarose gel electrophoresis using 2X RNA loading dye (R0641; Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... Libraries were quantified using Qubit Flurometer (Thermo Fisher Scientific) and profiled using Fragment Analyzer ...
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bioRxiv - Cancer Biology 2024Quote: ... cDNA was synthesized from 1 μg of total RNA using Maxima First Strand cDNA Synthesis Kit (K1672; Thermo Fisher Scientific). RT-qPCR was performed using Applied Biosystems™ PowerUP™ SYBR™ Green Master Mix (A25778 ...
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bioRxiv - Cancer Biology 2024Quote: ... RNAs were reverse transcribed using the EZ DNAse VILO IV kit (Invitrogen). 1.25 µL of cDNA was pre-amplified for 15-18 cycles in a multiplex reaction using Preamp Master-Mix solution (ref ...
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bioRxiv - Cancer Biology 2024Quote: ... and passed through a Hamilton syringe (14-824-663; Thermo Fisher Scientific) to dissociate proteins attached to chromatin ...
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bioRxiv - Cancer Biology 2024Quote: ... glass coverslips (ThermoFisher Scientific). After 48 h ...
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bioRxiv - Cancer Biology 2024Quote: ... UltraComp eBeads (01-2222; Thermo Fisher Scientific) and BD Horizon™ fixable viability stain 780 (565388 ...
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bioRxiv - Cancer Biology 2024Quote: ... in Prolong Glass Antifade mounting medium (ThermoFisher Scientific). Images were acquired on an LSM Olympus FV1000 spectral SIM confocal microscope (Tokyo ...
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bioRxiv - Cancer Biology 2024Quote: ... RT-qPCR was performed using Applied Biosystems™ PowerUP™ SYBR™ Green Master Mix (A25778; Thermo Fisher Scientific) and specific primers on a Stratagene Mx3005P Instrument (Agilent Technology ...
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bioRxiv - Cancer Biology 2024Quote: ... on the 7500 real-time PCR machine (Applied Biosystems). Data were normalized with the mean expression of household genes (HPRT ...