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Citations for Thermo Fisher :
151 - 200 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... cells were digested with TrypLE (Gibco, 12604013) and the tissues were removed by passing through a 70 µm cell strainer (Falcon ...
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bioRxiv - Evolutionary Biology 2024Quote: ... cells were co-transfected using Lipofectamine 3000 Transfection Reagent (Invitrogen) with plasmids expressing wildtype (WT ...
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Appropriate glycemic management protects the germline but not uterine environment in type 1 diabetesbioRxiv - Developmental Biology 2024Quote: ... Mounting was performed using ProLong Gold Antifade (Thermo Fisher Scientific) mounting media and the samples were stored at RT for 24 h and at 4 □C thereafter until imaging ...
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bioRxiv - Developmental Biology 2024Quote: The following siRNAs were purchased from Ambion: 5’ - GCUUGCCCGAGUUCU ACUAtt - 3’ (sense strand ...
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bioRxiv - Developmental Biology 2024Quote: ... or Alexa Fluor 647 goat anti-mouse IgG (Invitrogen A21235, 1:200). Counterstaining was then conducted using DAPI (Thermo Fisher Scientific 62248 ...
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bioRxiv - Developmental Biology 2024Quote: ... followed by mounting with Immu-Mount (Fisher Scientific, 9990402).
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Appropriate glycemic management protects the germline but not uterine environment in type 1 diabetesbioRxiv - Developmental Biology 2024Quote: ... using the RETCH MM 400 Mixer Mill (Fisher Scientific) for 4×15 s at 25 Hz ...
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bioRxiv - Developmental Biology 2024Quote: ... Sections were stained for BrdU (B35128, ThermoFisher), and the detection of EdU was performed with Click-iT assay (Invitrogen ...
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Appropriate glycemic management protects the germline but not uterine environment in type 1 diabetesbioRxiv - Developmental Biology 2024Quote: ... RNA quality was assessed using the Qubit RNA IQ assay kit (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2024Quote: Total RNA from whole cell lysate or specific subcellular fractions were extracted using TRIzol reagent (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... The cell culture medium contained αMEM (Gibco, 12571063) supplemented with 20% FBS (Biowest ...
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bioRxiv - Developmental Biology 2024Quote: ... sections were incubated for 2 h at room temperature with fluorescently conjugated secondary antibodies: Alexa Fluor 488 goat anti-rabbit IgG (Invitrogen A11008, 1:200) or Alexa Fluor 647 goat anti-mouse IgG (Invitrogen A21235 ...
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bioRxiv - Developmental Biology 2024Quote: ... the PBS was replaced with pre-warmed (43°C) 40% wHyb (2x SSC, Invitrogen; 40% deionized formamide ...
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bioRxiv - Developmental Biology 2024Quote: ... 100 U/ml penicillin and 100 µg/ml streptomycin (Gibco, 15140122). After 7 days ...
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bioRxiv - Developmental Biology 2024Quote: ... All the 3D reconstructions and sections were analyzed using AVIZO 9.4.0 (Thermo Fisher Scientific).
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Appropriate glycemic management protects the germline but not uterine environment in type 1 diabetesbioRxiv - Developmental Biology 2024Quote: ... the Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific) was used according to the manufacturer’s instructions ...
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Appropriate glycemic management protects the germline but not uterine environment in type 1 diabetesbioRxiv - Developmental Biology 2024Quote: ... The RNA was eluted in 30-50 µl Nuclease free water and quantified with Nanodrop 1000 spectrophotometer (ThermoFisher Scientific). RNA quality was assessed using the Qubit RNA IQ assay kit (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2024Quote: ... B27 Serum-Free Supplement (Gibco, 17504-044), 2 mM L-Glutamine (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2024Quote: ... Reverse transcribed using the TaqMan Reverse Transcription Reagent (Applied Biosystems). Quantitative PCR (qPCR ...
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bioRxiv - Developmental Biology 2024Quote: ... and Neurobasal medium (Gibco, 21103049) supplemented with N2 (homemade) ...
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bioRxiv - Developmental Biology 2024Quote: ... Concentration and purity (A260:A280) were determined by Qubit and Nanodrop (Thermo Fisher). DNA size range was determined using TapeStation (Agilent).
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bioRxiv - Developmental Biology 2024Quote: mESCs were plated on gelatin-coated plates at a final density of 1 × 104 cells/cm2 in N2B27 medium (1:1 mix of DMEM/F12 (Gibco, 21331020) and Neurobasal medium (Gibco ...
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bioRxiv - Developmental Biology 2024Quote: ... followed by Alexa Fluor 488–conjugated goat anti-rabbit antibody (1:500; A11070, Invitrogen, USA). Nuclei were stained with DAPI (40 ...
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bioRxiv - Developmental Biology 2024Quote: ... Whole genome-amplified DNA was quantified using the Qubit dsDNA Broad Range Assay kit (Invitrogen). Size distribution of amplicons was determined using the TapeStation Genomic DNA Screen Tape assay (Agilent) ...
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bioRxiv - Developmental Biology 2024Quote: ... Alexa Fluor 594 goat anti-rabbit IgG (H+L) (Invitrogen, A-11012, 1:2,000), and Alexa Fluor 594 goat anti-rat IgG (H+L ...
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bioRxiv - Developmental Biology 2024Quote: ... and 65µL of lysis buffer (Ambion RNAqueous-Micro Kit, ThermoFisher Scientific, cat # AM1931) was pipetted into the tube cap ...
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bioRxiv - Developmental Biology 2024Quote: ... and 65µL of lysis buffer (Ambion RNAqueous-Micro Kit ...
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bioRxiv - Developmental Biology 2024Quote: ... Alexa Fluor 594 goat anti-mouse IgG (H+L) (Invitrogen, A-11032, 1:2,000), Alexa Fluor 594 goat anti-rabbit IgG (H+L ...
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bioRxiv - Developmental Biology 2024Quote: ... the concentration of the HRT was adjusted to 100 ng/µl as measured with Nanodrop (Thermo Scientific). Guide RNAs were purchased from IDT either as sgRNA or as crRNA and resuspended in IDT Duplex buffer to a concentration of 100 µM or 200 µM ...
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Developmental toxicity of pre-production plastic pellets affects a large swathe of invertebrate taxabioRxiv - Developmental Biology 2024Quote: ... The secondary antibodies Alexa 488-conjugated goat anti-mouse and Alexa-568-conjugated goat anti-rabbit (Molecular Probes, Waltham, MA, USA) were diluted 1:400 and 1:1000 ...
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bioRxiv - Developmental Biology 2024Quote: ... using FAST SYBR Green master mix (Thermo Fisher), in triplicate wells ...
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bioRxiv - Developmental Biology 2024Quote: ... This PCR product was used as a transcription template to generate in vitro synthesized double stranded RNA using the MEGAscript RNAi Kit (Ambion). Purified double-stranded RNA (100 ng/μl ...
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bioRxiv - Developmental Biology 2024Quote: ... Alexa Fluor 568 goat anti-rabbit IgG (H+L) (Invitrogen, A-11016, 1:2,000), Alexa Fluor 594 goat anti-mouse IgG (H+L ...
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bioRxiv - Developmental Biology 2024Quote: ... Alexa Fluor 568 goat anti-mouse IgG (H+L) (Invitrogen, A-11031, 1:2,000), Alexa Fluor 568 goat anti-rabbit IgG (H+L ...
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bioRxiv - Developmental Biology 2024Quote: ... elegans RNA using SuperScript One-Step RT-PCR with Platinum Taq (Invitrogen). This PCR product was used as a transcription template to generate in vitro synthesized double stranded RNA using the MEGAscript RNAi Kit (Ambion) ...
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bioRxiv - Developmental Biology 2024Quote: ... Alexa Fluor 488 goat anti-rabbit IgG (H+L) (Invitrogen, A-11034, 1:2,000), Alexa Fluor 568 goat anti-mouse IgG (H+L ...
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bioRxiv - Developmental Biology 2024Quote: ... 100 μl of Dynabeads protein G (Thermo Fisher Scientific, 10765583) per sample were washed in ice-cold blocking solution (PBS containing 0.5% BSA ...
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bioRxiv - Evolutionary Biology 2024Quote: ... the left and right fore-wing buds were removed and transferred into 20 μl Trizol (Invitrogen). This procedure was repeated until a pool of either five (for i4E and i4L ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 1 uL 10 mM dNTPs (Thermo Scientific, USA, product #R0191), 1 uL of the genomic DNA extracted using a Wizard Genomic DNA Purification Kit according to the manufacturer’s instructions (A1120 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... DNA concentration was evaluated with Picogreen and Victor X2 fluorometry (Life Technologies, Carlsbad, CA), and an Agilent 4200 Tapestation (Agilent Technologies ...
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bioRxiv - Developmental Biology 2024Quote: ... DNA was prepared from individual animals and AccuprimeTM Pfx SuperMix (Invitrogen) was used to amplify target sites ...
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bioRxiv - Developmental Biology 2024Quote: ... RNA was extracted using Trizol (Ambion), and cDNA was made from RNA using qScript cDNA SuperMix (QuantaBio) ...
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bioRxiv - Developmental Biology 2024Quote: ... Linearized plasmid was run off with SP6 RNA polymerase using the mMESSAGE mMACHINE SP6 Transcription kit (Ambion). ptgs1 RNA was injected together with emx2 MO at one-cell stage at concentration 825 pg for rescue study.
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bioRxiv - Developmental Biology 2024Quote: ... and Alexa Fluor 594 goat anti-rat IgG (H+L) (Invitrogen, A-11007, 1:2,000).
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bioRxiv - Developmental Biology 2024Quote: ... Secondary antibodies used include Alexa Fluor 488 goat anti-mouse IgG (H+L) (Invitrogen, A-11029, 1:2,000), Alexa Fluor 488 goat anti-rabbit IgG (H+L ...
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bioRxiv - Developmental Biology 2024Quote: ... and Alexa Fluor 594 goat anti–rabbit (1:500; Life technologies A11037). For anti–6xHis-tag staining ...
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bioRxiv - Developmental Biology 2024Quote: ... Ultimately the best solution was found to be DiD’ (Life Technologies), a lipophilic ...
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bioRxiv - Developmental Biology 2024Quote: ... on vitronection (Thermo Fisher #15134499) coated plates ...
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bioRxiv - Developmental Biology 2024Quote: ... 1μg/mL anti-Ki-67 (Invitrogen; PA5-19462), 7μg/mL anti-Ac-alpha-Tubulin (K40) ...
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bioRxiv - Developmental Biology 2024Quote: ... mounted with ProLong Glass Antifade (Thermo Fisher; P36980), and allowed to cure overnight at RT in the dark ...