Labshake search
Citations for Thermo Fisher :
151 - 200 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Physiology 2024Quote: ... RNA quantity was assessed with Nanodrop spectrophotometry (Thermo Fisher Scientific, Wilmington, DE). Standard reverse transcriptase was performed using High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems by Life Technologies ...
-
bioRxiv - Physiology 2024Quote: ... Standard reverse transcriptase was performed using High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems by Life Technologies, Foster City, CA). Transcript mRNA levels were determined by qRT-PCR on the ABI Prism 7900HT Real Time System (Applied Biosystems ...
-
bioRxiv - Physiology 2024Quote: ... designed using Primer Express Software Version 3.0 (Applied Biosystems), for the genes measured by SYBR green are provided in Supplementary Table 2 ...
-
bioRxiv - Physiology 2024Quote: ... Transcript mRNA levels were determined by qRT-PCR on the ABI Prism 7900HT Real Time System (Applied Biosystems, Carlsbad, CA), using SYBR green (Qiagen ...
-
bioRxiv - Physiology 2024Quote: ... Standard reverse transcriptase was performed using High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems by Life Technologies, Foster City, CA). Transcript mRNA levels were determined by qRT-PCR on the ABI Prism 7900HT Real Time System (Applied Biosystems ...
-
bioRxiv - Physiology 2024Quote: ... phoSTOP phosphatase inhibitors and cOmplete protease inhibitors (ThermoFisher). Sample protein concentration was determined using a BCA kit (ThermoFisher/Pierce) ...
-
bioRxiv - Physiology 2024Quote: ... Samples were mounted using Fluoromount-G™ (Invitrogen, Thermo Fisher) and imaged with a Leica SPE confocal microscope (Leica Microsystems ...
-
bioRxiv - Physiology 2024Quote: ... Samples were mounted using Fluoromount-G™ (Invitrogen, Thermo Fisher) and imaged with a Leica SPE confocal microscope (Leica Microsystems ...
-
bioRxiv - Physiology 2024Quote: ... Cell lysates were collected and protein samples semi-purified by size-exclusion filtration using centrifugal concentrators with a polyethersulfone membrane (ThermoFisher Scientific, Waltham, MA). Specifically ...
-
bioRxiv - Physiology 2024Quote: Mice received a single intraperitoneal injection of 30 μg anti-CD3 antibody (Thermo Fisher Scientific) diluted to 200 μL with physiological saline ...
-
bioRxiv - Physiology 2024Quote: ... Digested tissues were immediately transferred to 10 μM BODIPY-FL-iodoacetamide (Thermo Fisher Scientific) in Dulbecco’s Phosphate-Buffered Saline (DPBS ...
-
bioRxiv - Physiology 2024Quote: ... Secondary antibodies from Invitrogen (Alexa Fluor 647 goat anti-rabbit ...
-
bioRxiv - Physiology 2024Quote: qPCR: Total RNA was isolated from cultured or ex vivo tissues using TRIzol (Invitrogen, MA, USA) or RNeasy Mini Kit (Qiagen ...
-
bioRxiv - Physiology 2024Quote: ... The pooled supernatants were dried up by Savant SpeedVac SPD120 (Thermo Fisher Scientific, MA, USA) and redissolved in DMSO ...
-
bioRxiv - Neuroscience 2024Quote: ... AAVs were applied at a flow-rate of 3 mL/min to a self-packed 1 mL column (POROSTM HQ 50 µm strong anion exchange resin, Thermo Fisher Scientific), which was equilibrated in buffer A ...
-
bioRxiv - Neuroscience 2024Quote: ... in a StepOnePlusTM Real-Time PCR System (Applied Biosystems). Relative expression of gene transcripts was assessed using the 2-Δ ΔCt method ...
-
bioRxiv - Neuroscience 2024Quote: ... the coverslips were incubated with secondary antibodies (α-rabbit IgG Alexa-conjugated 488 Invitrogen A11029 ...
-
bioRxiv - Neuroscience 2024Quote: ... and grown in Neurobasal plus medium (Invitrogen) supplemented with 2% B27 plus (Invitrogen) ...
-
bioRxiv - Neuroscience 2024Quote: ... Cells were maintained in DMEM (Gibco) supplemented with 10% FBS (Invitrogen ...
-
bioRxiv - Neuroscience 2024Quote: ... coverslips were mounted with Fluoromount (ThermoFisher Scientific).
-
bioRxiv - Neuroscience 2024Quote: ... A total of 1 μg of extracted mRNA was used to synthetize cDNA using SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher). Pcdh9 specific sequences were amplified from cDNA with SYBR Green PCR Master Mix (Applied Biosystems ...
-
bioRxiv - Neuroscience 2024Quote: ... 2 µg of total RNA was used for reverse transcription using the high Capacity cDNA Reverse Transcription Kit (Applied Biosystems) following the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2024Quote: ... 1% L-glutamine (Invitrogen), 1% penicillin/streptomycin (Invitrogen ...
-
bioRxiv - Neuroscience 2024Quote: ... qPCR was performed with PowerUpTM SYBRTM Green Master Mix (Applied Biosystems) in a StepOnePlusTM Real-Time PCR System (Applied Biosystems) ...
-
bioRxiv - Neuroscience 2024Quote: ... 100 U/ml penicillin (Gibco) and 100 μg/ml streptomycin (Gibco) ...
-
bioRxiv - Neuroscience 2024Quote: ... All chambers and tools were cleaned with RNase Away (Thermo Scientific) and 70% ethanol ...
-
bioRxiv - Neuroscience 2024Quote: ... pH 7.4 (Gibco). The brain was quickly extracted from the cranium ...
-
bioRxiv - Neuroscience 2024Quote: ... Enhanced Green fluorescent protein (GFP) was expressed from the pGREEN LANTERN™-1 vector (Life Technologies). The mCherry plasmid was obtained from Addgene (#84329 ...
-
bioRxiv - Neuroscience 2024Quote: ... and 100 μg/ml streptomycin (Gibco), incubated at 37 °C in a 5% CO2 atmosphere ...
-
bioRxiv - Neuroscience 2024Quote: ... 100U/ml penicillin (Gibco), 100 mg/ml streptomycin (Gibco ...
-
bioRxiv - Neuroscience 2024Quote: ... 100 mg/ml streptomycin (Gibco) and plated on poly-L-lysine-coated glass coverslips ...
-
bioRxiv - Neuroscience 2024Quote: ... 100 μL of 200mg/ml 5-ethynyl-2’-deoxyuridine (EdU; E10187, ThermoFisher Scientific) was administered daily for 2 weeks by intra perennial injections into 4 week ...
-
bioRxiv - Physiology 2024Quote: ... 2 µl cDNA was amplified with TaqMan™ qPCR (38 cycles, 3 technical replicates of 3 biological replicates) using master mix (Thermo Fisher) and the following TaqMan assays ...
-
bioRxiv - Physiology 2024Quote: ... ΔCT values were calculated with mean sample CT normalized to mean CT values of the endogenous housekeeping gene HPRT (Thermo Fisher, #Mm03024075_m1, VIC) detected via Multiplex qPCR.
-
bioRxiv - Physiology 2024Quote: The gene expression in transduced mFB was investigated by qPCR using TaqMan assays (Thermo Fisher). For this ...
-
bioRxiv - Physiology 2024Quote: ... slides were covered aqueous (Aqua Polymount, Thermo Fisher) and imaged using confocal microscopy (Eclipse Ti ...
-
bioRxiv - Physiology 2024Quote: ... 250 ng RNA was transcribed into cDNA using the SuperScriptVILO Kit (Thermo Fisher). Then ...
-
bioRxiv - Neuroscience 2024Quote: ... DRG neurons or HEK293 cells were incubated with 5 μM Fura-2 AM and 0.2% Pluronic (Thermo Fisher Scientific) (prepared from a 200 mg/ml stock solution in DMSO ...
-
bioRxiv - Neuroscience 2024Quote: ... 2 μl of Lipofectamine 2000 was mixed with the DNA in 100 μl of OptiMem (Thermo Fisher Scientific), a reduced serum media ...
-
bioRxiv - Neuroscience 2024Quote: ... The explants were dissociated into a single-cell suspension using 0.25%Trypsin (ThermoFisher Scientific SKU #15400054) and then dissociated by trituration after washes ...
-
bioRxiv - Neuroscience 2024Quote: ... incubating brain sections with Click-iT Plus reaction cocktail (made following the manual on the Click-iT Plus EdU Imaging Kits, C10637, ThermoFisher Scientific) for 30 minutes protected from light at room temperature ...
-
bioRxiv - Neuroscience 2024Quote: ... A 2 × 2 cm piece of Parafilm M (Fisher Scientific) was carefully placed onto the tissue so that the gene panel mix was evenly spread across the tissue and no bubbles were introduced between the tissue and the film ...
-
bioRxiv - Neuroscience 2024Quote: ... with Neurobasal medium (ThermoFisher Scientific SKU #21103049) containing B-27 supplement (ThermoFisher Scientific SKU #17504044 ...
-
bioRxiv - Neuroscience 2024Quote: ... Neurog2 (ThermoFisher Scientific; PA5-78556, 1:1000), β Catenin (BD Biosciences ...
-
bioRxiv - Physiology 2024Quote: ... 25 mM HEPES (Gibco), and 100 U/mL penicillin/streptomycin (Gibco) ...
-
bioRxiv - Physiology 2024Quote: ... 2 mM GlutaMAX (Gibco), 25 mM HEPES (Gibco) ...
-
bioRxiv - Physiology 2024Quote: ... 10 mM HEPES (Gibco) and 0.25 μg/mL amphoterecin B at 37°C with 5% CO2 for 12 - 48 hours.
-
bioRxiv - Physiology 2024Quote: ... and 100 U/mL penicillin/streptomycin (Gibco). Detailed human islet donor information is listed in Supplementary Table 3.
-
bioRxiv - Physiology 2024Quote: ... 100 U/mL penicillin/streptomycin (Gibco), 1 mM sodium pyruvate (Cellgro) ...
-
bioRxiv - Physiology 2024Quote: Mouse pancreata or human and mouse islet sections were then washed with PBS for 5 minutes and permeabilized and blocked with 1% normal donkey serum and 0.15% Triton X-100 (Fisher Scientific) in PBS for 1 hour ...