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Citations for Thermo Fisher :
1351 - 1400 of 1859 citations for N Acetyl Tizanidine d4 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Proteins were resolved on precast NuPAGE Novex 4-12% Bis-Tris Gels in 3-(N-morpholino)propanesulfonic acid (MOPS) buffer (Invitrogen). Samples were transferred to 0.45 µm nitrocellulose membrane (0.9 A ...
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bioRxiv - Cell Biology 2024Quote: ... at 4°C o/n and then with 40 μL of pre-blocked (1 mg/ml BSA) Dynabeads protein A (ThermoFisher). Beads were sequentially washed with Wash buffer 1 (20 mM Tris HCl pH 8.1 ...
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bioRxiv - Developmental Biology 2023Quote: ... Membranes were blocked for 2hrs at RT in 5% milk powder and then incubated overnight (o/n) at 4°C with primary rabbit anti-GFP antibody (1:1,000; Molecular Probes). Membranes were washed 3x for 10min in 1X TBST ...
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bioRxiv - Neuroscience 2023Quote: ... A 6xHis-myc tag or GFP was attached to the N-terminus and the construct was cloned into the pSecTag2A vector (Invitrogen) in frame with the N-terminal IgK signal sequence ...
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bioRxiv - Bioengineering 2024Quote: WT IL-10 and Fab-IL-10 constructs were fluorescently labeled using Alexa Fluor 647 N-hydroxysuccinimide (NHS) ester (Invitrogen), and unreacted dye was removed with a Zeba Spin Desalting Column (7K MWCO ...
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bioRxiv - Biochemistry 2024Quote: ... Labeling of WT and p.R196H actin at cysteine–374 using N–(1–pyrene)iodoacetamide (Thermo Fisher Scientific, Waltham, MA, USA) followed established protocols (55).
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bioRxiv - Neuroscience 2023Quote: ... and incubated in culture media (66% BME, 25% Hanks, 5% FBS, 1% N-2, 1% penicillin, streptomycin and glutamine; all from Invitrogen) and 0.66% D-(+)-Glucose (Sigma ...
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bioRxiv - Molecular Biology 2023Quote: ... Experiments were carried out using 8 μL of SETDB1-3TD (residues 196-403, N-terminal His tag) and 20X concentration Sy-pro Orange dye (5000X DMSO stock, ThermoFisher) and 2 uL of ligand for a final concentration of 20 μM of protein and 200 μM of ligand with 1.1% of DMSO ...
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bioRxiv - Neuroscience 2022Quote: ... total proteins from RVLM-containing tissue punches were extracted using N-PER™ Neuronal Protein Extraction Reagent (Thermo Fisher Scientific) following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Sequences of full-length Dprs and DIPs with an N-terminal V5 tag were cloned into the pMT/BiP/V5-His A vector (Invitrogen) for expression in S2 Drosophila cells for PLC cleavage assay or into the pAcGP67 baculovirus transfer vector (BD Biosciences ...
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bioRxiv - Molecular Biology 2022Quote: ... zipper tag via a 5XGGS linker region was co-transfected with N-terminally His-tagged human Leptin in suspension HEK293 FreeStyle cells (ThermoFisher) in the presence of 20 μM kifunensine (Dextra ...
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bioRxiv - Neuroscience 2023Quote: ... and the QOLG-1 wild-type parent line (n=1) were karyotyped using a commercial service that provides information on chromosomal changes >1Mb resolution (KaryoStat Assay, ThermoFisher). Briefly ...
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bioRxiv - Molecular Biology 2023Quote: ... and once with 1X PBS for 5 min each before blocking with 100 mM N-succinimidyl (acetylthio) acetate (NHS-acetate, ThermoFisher) in NHS-acetate buffer (0.1M NaP ...
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bioRxiv - Immunology 2023Quote: ... which was followed by an overnight incubation at 4°C with N-hydroxysuccinimide ester derivatives of AF647 (Thermo Fisher Scientific) present in two-fold molar excess.
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bioRxiv - Neuroscience 2023Quote: RNA was extracted from dissected hippocampal brain tissue (n = 3 females/genotype and age) preserved in RNAlater (AM7020, Thermo Scientific) using RNeasy Lipid Tissue Mini Kit (74804 ...
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bioRxiv - Pathology 2023Quote: ... LMD samples (n=5 for each sample structure) were pooled by adding 60 μl of acetonitrile (10055454, Thermo Fisher Scientific) to each tube and spun down ...
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bioRxiv - Cell Biology 2023Quote: ... Domes were polymerised by incubation for 15 minutes at 37°C and Advanced DMEM media supplemented with 1x N-2 (Gibco), 1x B-27 (Gibco) ...
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bioRxiv - Physiology 2023Quote: ... and clinical CRS-isolates (Drs. N. Cohen and L. Chandler, Philadelphia VA Medical Center [56]) were grown in Luria broth (Gibco). For anti-bacterial assays ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... Secondary antibodies were: goat anti-rat Alexa Fluor 647 (1:500, for anti-N-Cadherin, Life Technologies, cat. no. A21247), goat anti-rabbit Alexa Fluor 488 (1:500 ...
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bioRxiv - Cell Biology 2023Quote: Full-length cDNA sequence of Hs WDR23 Isoforms 1 and 2 were cloned into pcDNA 6.2/N-EmGFP/TOPO (Thermo Fisher), as previously described [19] ...
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bioRxiv - Molecular Biology 2023Quote: ... and from the liver graft of living donors (N=4) were obtained at the time of liver transplantation and stored frozen in RNAlater solution (Invitrogen). ABCB4 pathogenic mutations were documented in all PFIC3 by genetic analysis ...
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bioRxiv - Immunology 2023Quote: ... and stained for SARS-CoV-2 N protein using Biotin-labelled-CR3009 antibody produced in-house together with a Streptavidin-Alexa488 (S32354, Invitrogen) and cellular DNA using DAPI (10236276001 ...
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bioRxiv - Neuroscience 2022Quote: ... the medium was changed to neural induction media (1:1 mixture of DMEM/F-12 GlutaMAX: Neurobasal media supplemented with 0.5x N-2 (Life Technologies), 0.5x B-27 (Life Technologies) ...
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bioRxiv - Immunology 2022Quote: ... sheared gDNA of each sample was split in two duplicates and samples were incubated o/n at 65°C after mixing with an equal volume hybridization buffer (1 ml composition: 667 µl 20x SSPE (Gibco); 267 µl 50x Denhardt’s solution (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2022Quote: ... The cells were treated with either DMSO or 5 μM GolgiTracker ((BODIPY™ FL C5-Ceramide (N-(4,4-Difluoro-5,7-Dimethyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Pentanoyl)Sphingosine)) (Invitrogen. Cat# D3521) for 30 minutes at 37°C ...
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bioRxiv - Bioengineering 2022Quote: ... formalin-fixed fibrotic capsule tissue samples (n=2 per group) and subcutaneous tissue samples (n=2) were placed in a solution of 2 mg/ml FITC (Invitrogen) in DMEM/F12 media (Gibco) ...
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bioRxiv - Cell Biology 2022Quote: Cellular glucose uptake was measured by culturing cells in glucose free DMEM for 2 hours before adding 100µM of the fluorescent glucose analogue 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl) Amino)-2-Deoxyglucose (2-NBDG; ThermoFisher, N13195). The analogue was incubated with MCF7s for 30 minutes and MSCs for 60 minutes at 37°C ...
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bioRxiv - Biophysics 2023Quote: ... Cy3B and AF647 free dyes were prepared by hydrolyzing the corresponding NHS ( N-hydroxysuccimidyl) esters (PA63101, Cytiva, and A200006, Invitrogen) in 0.1 M sodium bicarbonate overnight ...
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bioRxiv - Genetics 2023Quote: ... Quantitative real time PCR (n = 7–8 per genotype) was performed on an ABI 7300 Sequence Detection system (Applied Biosystems) using the PowerUp SYBR Green Master Mix Kit (Applied Biosystems).
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bioRxiv - Cell Biology 2023Quote: Generation of replication-defective recombinant adenovirus expressing N-SREBPs under CMV promoter was performed using ViraPower Adenoviral Expression System (#K4930-00, Invitrogen) as described earlier (54) ...
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bioRxiv - Bioengineering 2023Quote: ... They were cultured on 100-mm Petri dishes coated with human VTN-N truncated recombinant protein (Gibco, Thermo Fisher Scientific). The hiPSCs obtained from Thermo Fisher Scientific were maintained in Essential 8 Medium (Gibco ...
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bioRxiv - Cell Biology 2022Quote: ... HEK293 cells stably expressing N-terminally FLAG-tagged DOR or MOR were cultured in the presence of 250 μg/ml Geneticin (Gibco). For transient DNA expression ...
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bioRxiv - Bioengineering 2023Quote: ... They were cultured on 100-mm Petri dishes coated with human VTN-N truncated recombinant protein (Gibco, Thermo Fisher Scientific). The hiPSCs obtained from Thermo Fisher Scientific were maintained in Essential 8 Medium (Gibco ...
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bioRxiv - Neuroscience 2023Quote: ... 30 mg of tissue per sample was homogenized in N-PER Neuronal Protein Extraction Reagent (Thermo Fisher Scientific, Cat. # 87792) with protease and phosphatase inhibitors (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... stained with N-(3-triethylammonium propyl)-4-(4-(dibutyl amino) styryl) pyridinium dibromide (FM 1-43FX, Invitrogen, Cat.-No. F35355) at 5.6 µg ml-1 ...
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bioRxiv - Cancer Biology 2023Quote: ... 0.1M Tris-base in DEPC H2O) and 1X PBS for 5 min each before being blocked using 100 mM N-succinimidyl acetate (NHS-acetate, ThermoFisher) in NHS-acetate buffer (0.1M NaP ...
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bioRxiv - Immunology 2023Quote: Following euthanasia, adult zebrafish heads (15 wpf, N=3) were fixed in a solution of 4% methanol-free formaldehyde (Thermofisher) in 60 mM HEPES buffer (pH 7.4 ...
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bioRxiv - Biophysics 2023Quote: ... incubated in 10 mg/mL sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate (sulfo-SMCC, Thermo Fisher Scientific, Waltham, MA, USA) at room temperature for 30 min ...
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bioRxiv - Molecular Biology 2023Quote: ... as already described [17] and reseeded onto 0.2 mm bone slices labelled with N-hydroxysuccinimide ester-activated rhodamine fluorescent dye (ThermoFisher Scientific), at a cell density of 1x105 cells/bone slice (Boneslices.com) ...
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bioRxiv - Plant Biology 2023Quote: ... For C-terminal fusions the CDS of PYE and OLV versions were amplified from cDNA of Fe deficient Arabidopsis WT roots with primer pairs PYE_B1 fw/PYEns_B2 rev and OLV_B1 fw/OLVns_B2 rev carrying B1 and B2 attachment sites without stop codon or from existing pDONR with N-terminal fusion (Supplemental Table S1) and transferred via Gateway cloning into pDONR207 (Invitrogen) according to the manual (Gateway ...
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bioRxiv - Molecular Biology 2023Quote: ... The flow-through was dialysed O/N at 4°C in a SnakeSkin™ Dialysis Tubing (3.5K MWCO, Thermo Scientific) against dialysis buffer (50 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Microbiology 2023Quote: ... N (75 fmol) and L (25 fmol) proteins and the T7 RNA polymerase (50 fmol) using lipofectamine 3000 (Invitrogen™) for 3 h at 37°C ...
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bioRxiv - Bioengineering 2023Quote: Blood plasma extracted from each group (n=5) was analyzed using the Inflammation 20-Plex Human ProcartaPlex™ Panel (Invitrogen). Briefly ...
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bioRxiv - Developmental Biology 2024Quote: The PROX1 cDNA was subcloned into pFASTBAC1 to express PROX1 with an N-terminal FLAG- tag in Sf9 cells by the baculovirus expression system (Invitrogen). The PROX1-FLAG protein was then purified on M2 agarose (Sigma) ...
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bioRxiv - Biochemistry 2024Quote: ... was serial diluted using a solution containing 0.1% FA in LCMS grade water (Pierce 85170) and 0.1% n-Dodecyl-beta-Maltoside Detergent (DDM, Thermo Fisher, 89902). A total of 10 microliters of each dilution ...
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bioRxiv - Plant Biology 2024Quote: ... The percentage of C and N were quantified using the FLASH 2000 Organic Elemental Analyzer (Thermo Fisher Scientific Villebon, France) and the 15N enrichment using the Delta V Advantage isotope ratio mass spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: BRSK1 and 2 were cloned into pDest vectors (to express N-terminal Flag or HA tagged proteins) using the Gateway LR Clonase II system (Invitrogen) as per the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... n=15 full larvae) or extracted from hCMEC/D3 cells seeded in 12 well/plates using a standard TRIzol (Invitrogen) method ...
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bioRxiv - Biochemistry 2024Quote: ... The DNA synthesis of calpL fused with TEV-cleavable His6-StrepII-His6 tag at the N- or C-terminal and cloning into pBAD/HisA vector (Invitrogen) was ordered from Twist Bioscience ...
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bioRxiv - Cell Biology 2024Quote: ... WT and YAP1-KO EHTs were snap frozen in liquid nitrogen and stored at −80 °C before thawing them O/N at −20 °C in RNA LATER-ICE (ThermoFisher). RNA was extracted using High Pure RNA Isolation Kit (Roche ...