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Citations for Thermo Fisher :
1401 - 1450 of 1859 citations for N Acetyl Tizanidine d4 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... WT and YAP1-KO EHTs were snap frozen in liquid nitrogen and stored at −80 °C before thawing them O/N at −20 °C in RNA LATER-ICE (ThermoFisher). RNA was extracted using High Pure RNA Isolation Kit (Roche ...
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bioRxiv - Biophysics 2020Quote: ... Protein solutions were then allowed to fibrillate O/N at 37°C with 900 rpm shaking in a table Eppendorf shaker (Thermo Scientific). After 24 h incubation ...
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bioRxiv - Biophysics 2019Quote: ... and subsequently cloned into CMV-mCherry-Gateway vector or Vivid Colors™ pcDNA™6.2/N-EmGFP-DEST vector (ThermoFisher Scientific) to obtain CMV-mCherry-DBNL or CMV-EmGFP-DBNL ...
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bioRxiv - Cell Biology 2020Quote: ... Germany) followed by staining with Oregon Green 488 goat anti-rabbit IgG (cat. n. O-11038, Molecular Probes, Leiden, The Netherlands). Microglia cells were plated in astrocyte-conditioned high glucose-DMEM medium (cat n ...
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bioRxiv - Cell Biology 2020Quote: ... vector and had a stop codon inserted at residue 90 to create the N-cTnC construct using the Phusion site-directed mutagenesis protocol (Thermo Scientific). This construct was transformed into the BL21(DE3 ...
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bioRxiv - Cell Biology 2020Quote: ... 12×106 Sf9 cells (1ml) were transfected with 5μg of purified bacmid using 15μl of Cellfectin™ (Invitrogen, P/N 58760). After 5h incubation ...
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bioRxiv - Genetics 2021Quote: ... RNA was extracted from control and CRISPR-Cas9 targeted Calu-3 cells (N = 3 biological replicates, with 3 technical replicates per experiment per condition) and prepared using Trizol Reagent (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2021Quote: ... Xenopus tropicalis R651X and a nuclear-localized β galactosidase (n gal) lineage tracer were synthesized in vitro according to β manufacturer’s protocols (mMessage mMachine kit, Ambion). Antisense RNA probes for in situ hybridization (ISH ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Ovipositors of wild and lab-reared flies (n ≤ 10 per species) were mounted onto slides with Permount mounting medium (Fisher Scientific) and coverslips ...
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bioRxiv - Developmental Biology 2021Quote: ... testes were collected from Dnmt3AKO and WT littermate animals at 19dpp and 25dpp (n=2 per genotype and age) and homogenized in Trizol (Life Technologies) using TissueLyser LT (Qiagen) ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples were separated on 4–12% NuPAGE® Bis-Tris gradient gels (1.0 mm x 12 well) (Invitrogen, Cat n°NP0321) using MOPS SDS Running Buffer (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: ... recombinant baculovirus containing the sequence of ΔPRD-dynamin 1 with an N-terminal His-tag was generated by following Bac-to-Bac Baculovirus Expression System (ThermoFisher Scientific). The suspension cultures of Sf9 were maintained in Sf-900 III serum-free media (SFM ...
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bioRxiv - Genetics 2019Quote: ... n = 4) for 24 hours and processed for RNA isolation and reverse transcription with the Cells-to-Ct Kit (Life Technologies), following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... The X-Y resolution of N-SIM was calibrated to 120-150 nm using 20 nm FluoSphere beads (Thermo Scientific, F887) in 580 nm laser ...
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bioRxiv - Bioengineering 2019Quote: ... 1.0 x105 cells were seeded in 12-well plates (n=3/condition/cell-type) and cultured in monolayer in differentiation media (StemPro Osteogenic Media; ThermoFisher Scientific) for 28 days ...
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bioRxiv - Cell Biology 2019Quote: The staining of limiting membrane of the yeast vacuole was achieved with FM™ 4-64 Dye (N-(3-Triethylammoniumpropyl)-4-(6-(4-(Diethylamino) Phenyl) Hexatrienyl) Pyridinium Dibromide) (Invitrogen). Cells were grown to early-log phase in appropriate medium ...
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bioRxiv - Bioengineering 2020Quote: ... Biotinylated mPA was then modified with 10 equivalents of sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate (sulfo-SMCC; Thermo Fisher Scientific) at r.t ...
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bioRxiv - Microbiology 2019Quote: ... Neural maintenance medium base (50% DMEM-F-12 medium-GlutaMAX, 50% Neurobasal-A medium, 1 × N-2 supplement, 1 × B-27 supplement [Life Technologies]) supplemented with 1% DMSO (Sigma ...
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bioRxiv - Synthetic Biology 2020Quote: ... DNA coding for three GFP-split intein fusion fragments (N, X and C) was inserted into the pcDNA3.1+ vector by GeneArt (Thermo Fisher Scientific) and co-transfected in a 1:1:1 ratio using a total of 3 µg DNA and incubated for 48 hrs ...
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bioRxiv - Biochemistry 2019Quote: ... Appropriate correction factors for the individual TMT channels for both peptide N-terminal labelling and lysine side-chain labelling as per the TMT kits used (Thermo Scientific) were configured into the integrated Andromeda search prior to the MaxQuant search.
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bioRxiv - Physiology 2020Quote: ... Samples were then derivatized with 25 µL of N,O-bis (Trimethylsilyl)trifluoroacetamide with Trimethylchlorosilane (BSTFA with 1% TMCS, Thermo Scientific) for 1h at 37°C ...
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bioRxiv - Bioengineering 2019Quote: Codon-optimised gene synthesis of the following N-terminally truncated (ΔN) FAP enzymes was performed by GeneArt (Thermo Fisher; Table S12): CvFAPWT from Chlorella variabilis NC64A11 (Genbank ...
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bioRxiv - Bioengineering 2019Quote: ... and Ana GVs with GvpC removed (AnaΔC) were prepared as previously described.34 Dylight415-Co1 N-hydroxysuccinimide ester (Thermo Fisher Scientific) was reacted with GVs in PBS for 2 hours at 10,000:1 molar ratio ...
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bioRxiv - Molecular Biology 2020Quote: ... was used to generate amplified and biotinylated sense-strand cDNA from the entire expressed genome according to the GeneChip WT PLUS Reagent Kit User Manual (P/N 703174 Rev 1; Affymetrix Inc.). GeneChip HTA Arrays were hybridized for 16 h in a 45 °C oven ...
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bioRxiv - Bioengineering 2020Quote: ... The efficiency of siRNA encapsulation N+:P− ratios was evaluated using a Quant-iT Ribogreen assay kit (ThermoFisher Scientific, Waltham, MA). siNP size was evaluated using dynamic light scattering (DLS ...
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bioRxiv - Microbiology 2021Quote: ... Approximately 2.5 μg of the in vitro synthesized RNA was used to transfect ∼6 ×105 BHK-hACE2-N cells stably expressing the SARS-CoV-2 N and the human ACE2 genes 45 using the MessengerMax lipofection kit (Thermo Scientific) as per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... The coding sequence of human PLIN2 with an N-terminal EGFP tag was provided by John McLauchlan (Targett-Adams et al., 2003) and re-cloned into pCR8 (ThermoFisher Scientific). The human PLIN3 (TIP47 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... from regenerating tails at 14 dpa (n = 3) were obtained on a Leica CM1850 UV cryostat and positioned on the Color Frost Plus microscope slides (Thermo Fisher). Sections were fixed as previously described [35] and stored at −80°C for hematoxylin and eosin staining or in situ hybridization ...
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bioRxiv - Biochemistry 2020Quote: ... and cleavage products were detected using mouse monoclonal BC-3 antibody which detects aggrecan cleavage at the Glu392↓Ala393 bond (Cat n.: MA316888, Life Technologies). Immobilon Chemiluminescent HRP substrate (Cat ...
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bioRxiv - Physiology 2020Quote: All 7 gills from each individual shrimp (5 ≤ N ≤ 8) were dissected under a magnifying lens with micro-scissors and homogenized in TRIzol reagent (Life Technologies, Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... EC-N-Cdh protein purified from wild type and ΔPOMGnT1 HEK293T cells (see above) was incubated with pre-washed Dynabeads™ (Invitrogen) at a ratio of 40 μg of protein per 40 μl of bead suspension o/n at 4 °C ...
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bioRxiv - Bioengineering 2021Quote: ... Three images of three independent experiments (n = 3) of as-made scaffolds as well as those cultured to day 21 were captured using EVOS M5000 (ThermoFisher, Canada), and were quantified using the cell counter plug-in on ImageJ (NIH ...
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bioRxiv - Microbiology 2020Quote: ... A standard curve was produced using serial 10-fold dilutions of in vitro transcribed RNA of N gene driven by the SP6 promoter (ThermoFisher #AM1340). Viral burden was expressed on a log10 scale as viral RNA copies per g of tissue or ml of serum ...
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bioRxiv - Microbiology 2021Quote: ... Approximately 2.5 μg of the in vitro synthesized RNA was used to transfect ∼6 ×105 BHK-hACE2-N cells stably expressing the SARS-CoV-2 N and the human ACE2 genes(Rihn et al., 2021) using the MessengerMax lipofection kit (Thermo Scientific) as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... and contained 5 µl PowerUp SYBR Green Master Mix with UNG (uracil-N glycosylase to prevent carryover contamination; Thermo Fisher Scientific), 1 µM (panC ...
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bioRxiv - Cell Biology 2020Quote: ... and Ctns-/- (n=6) mice were euthanized and the kidneys immediately removed and stored at −80°C in RNA Later (Life Technologies). Tissues were subsequently ground using Precellys 24 (Bertin Technologies ...
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bioRxiv - Biophysics 2021Quote: Human wild type ABCG2 or ABCG2 R184A containing an N-terminal Flag-tag was expressed in HEK293-EBNA (Thermo Fisher Scientific) cells as previously described19 ...
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bioRxiv - Cell Biology 2021Quote: ... The pcDNA5/FRT-PRPF4 plasmid was constructed by cloning the PRPF4 gene fused to an N-terminal FLAG/HA tag into the pcDNA5/FRT plasmid (Thermo Fisher).
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bioRxiv - Animal Behavior and Cognition 2022Quote: Groups of 16 larvae at 5 dpf from all corresponding genotypes (N = 3 per genotype) were collected and stored in RNAlater (Thermo Fisher) until use ...
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bioRxiv - Cell Biology 2022Quote: ... lines were generated in HEK293T cells expressing retroviral packaging plasmids (gift from N. Montserrat) and transfected using Lipofectamine™ 3000 Transfection Reagent (Invitrogen).
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bioRxiv - Immunology 2022Quote: ... we resuspended cells at a concentration of 1000 cells/µL and added 1µl of RNase Inhibitor (Invitrogen, Cat. N.10777-019) before loading the mix on a Chromium Comptroller Instrument (10x Genomics) ...
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bioRxiv - Biochemistry 2022Quote: ... was cloned in frame with a N-terminal GFP tag and intervening short peptide linker (GSGEGRG) into the pcDNA5/FRT/TO bacterial expression vector (Invitrogen; V652020). A C-terminal histidine tag (Hisx6 ...
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bioRxiv - Cancer Biology 2022Quote: ... Pierce premium grade N-hydroxysulfosuccinimide (sulfo-NHS, PG82071) and 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC, 22980) were purchased from Thermo Fisher Scientific.
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bioRxiv - Microbiology 2022Quote: ... The levels of RNA corresponding to the N protein-encoding gene of SARS-CoV-2 were measured using the TaqMan Fast Virus 1-step Master Mix (Thermo Scientific). Each 20-μL reaction mixture contained 5.0 μL of 4× TaqMan Fast Virus 1-Step Master Mix ...
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bioRxiv - Microbiology 2020Quote: ... cells were immunofluorescently labelled for viral N-protein (detected with AlexaFluor488 or AlexaFluor546 conjugates) followed by RNA visualisation using the ViewRNA Cell Plus Kit (Thermo Fisher). The ViewRNA probes implemented targeted IL-6 (VA4-19075 ...
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bioRxiv - Microbiology 2020Quote: ... a codon optimized cDNA sequence for the ORF of SARS-CoV-2 N (NCBI reference sequence number: NC_045512) was cloned into the pEGFP-C1 (by GeneArt-Thermo Fisher Scientific). Once cloned ...
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bioRxiv - Immunology 2021Quote: ... GenBank: QHD43416.1) with an N-terminal IgKappa signal sequence (METDTLLLWVLLLWVPGSTG) and C-terminal TwinStrep- and 3xFLAG-tags (GeneArt, ThermoFisher, Regensburg, Germany) into a pcDNA3.4(+ ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were washed and mounted in media (0.5% N propyl gallate, 70% glycerol, 1M tris pH8.0) containing 4′,6-diamidino-2-phenylindole (DAPI) (Invitrogen, Cat. No. D1306).
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bioRxiv - Neuroscience 2021Quote: 6 week old female wild-type and TREM2-IPD mice (n=3) were decapitated and brains were dissected into freshly prepared dissection medium containing 2X MEM (Gibco, 04195120M), 25mM HEPES ...
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bioRxiv - Cell Biology 2020Quote: ... 100 μg of anti-rabbit IgG was incubated with 8 μg of Alexa Fluor 750 N-Succinimidyl Ester (Thermo Fisher Scientific) in 100 mM NaHCO3 (pH 8.3 ...