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Citations for Thermo Fisher :
1301 - 1350 of 1859 citations for N Acetyl Tizanidine d4 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: ... the insert from the entry clone was transferred to the destination clone Vivid Colors pcDNA 6.2/N-EmGFP-DEST (Life Technologies) via a Gateway LR Clonase reaction to produce the following plasmids ...
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bioRxiv - Microbiology 2020Quote: ... and posteriorly cleaved with BamHI/PstI restriction enzymes and inserted in-frame to an N-terminal polyhistidine tag in the expression vector pRSETA (Invitrogen), previously digested with the same enzymes ...
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bioRxiv - Microbiology 2020Quote: ... in vitro transcription of the ligated cDNA (along with a N-gene containing cDNA) using an mMachine T7 transcription kit (Ambion), and subsequent electroporation into BHK-R cells ...
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bioRxiv - Microbiology 2020Quote: ... The reaction was stopped by addition of 0.5 N HCl and absorbance at 450 nm was measured using a Muktiskan FC microplate photometer (Thermo Scientific).
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bioRxiv - Biochemistry 2021Quote: ... The resulting PCR product (N linker::CeTurboID::c-Myc::mNeongreen::3XFLAG) was cloned into position 2 of a Multisite Gateway pDONR vector (ThermoFisher).
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bioRxiv - Immunology 2021Quote: ... The SARS-CoV-2 N standards were generated by in-vitro transcription of the generated SARS-CoV-2 N cDNA using the MegaScript T7 Transcription kit (Invitrogen), followed by using the MEGAclear Transcription Clean-Up Kit ...
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bioRxiv - Neuroscience 2019Quote: ... 150 ng of total RNA extracted from the brainstem of Vglut2:Ndufs4cKO (n=4) and Vglut2:Ndufs4cCT mice (n=4) was amplified and biotin-labeled using the Illumina TotalPrep RNA Amplification kit (Ambion). 750 ng of the labeled cRNA was hybridized to MouseRef-8 v2 expression beadchips (Illumina ...
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bioRxiv - Evolutionary Biology 2019Quote: ... We subsequently placed two frozen individuals from each family and treatment group (n = 9 families * 2 treatments (+/− supplemental thiamine) * 2 individuals = 36) into 10 volumes of RNAlater-ICE (Invitrogen) pre-chilled to −80 °C and allowed the samples to reach −20 °C overnight ...
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bioRxiv - Microbiology 2021Quote: ... and cDNAs were synthesized using SARS-CoV-2 nucleocapsid (N) reverse primer N660R (5’-AGCAAGAGCAGCATCACCGCCATTGCCAGC-3’) and M-MLV reverse transcriptase (Invitrogen). Then ...
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bioRxiv - Immunology 2021Quote: Codon-optimized sequences for the N or the spike S protein of SARS-CoV-2 were cloned into the pVAX1 expression plasmid (ThermoFisher) optimized for DNA vaccinations referred to as pVAX1-SARS2-N and pVAX1-SARS2-S 81 ...
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bioRxiv - Cell Biology 2021Quote: ... and recombined with an RPS5A::XVE promoter and an N-terminal GFP in a pB7m34GW backbone (Karimi et al., 2007) via an LR reaction (Invitrogen) and verified by restriction digest.
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bioRxiv - Physiology 2020Quote: ... and incubated with 100 mM 6-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (6-NBDG) (Life Technologies) in 10 nM Tris/HEPES buffer containing 150 mM KCl or 150 mM NaCl for 30 minutes at 37 °C ...
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bioRxiv - Molecular Biology 2020Quote: Plasmid encoding the N-terminally His-tagged Msm HelD protein was prepared by the GeneArt® Plasmid Construction Service (Thermofisher). Gene construct for HelD expression was designed by codon-optimized back translation of gene MSMEG_2174 from Msm (strain ATCC 700084 / mc2 155 ...
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bioRxiv - Molecular Biology 2019Quote: ... 6X N-terminally Myc-tagged cDNAs encoding Ku70 or Ku80 WT and mutants were cloned into pcDNA/FRT/TO (Invitrogen) and then transfected along with Flp recombinase expressing pOG44 plasmid using Lipofectamine transfection reagent (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... was crosslinked to the PAAm gels using N-sulfosuccinimidyl-6-(4′-azido-2′-nitrophenylamino)hexanoate (sulfo-SANPAH, from Life Technologies). 30 μL of 1mg/mL sulfo-SANPAH dissolved in milliQ H2O was added to PAAm gels which were irradiated under a 365nm UV lamp (ABM ...
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bioRxiv - Biochemistry 2021Quote: DNA sequences encoding N-terminally GFP-fused and truncated FAM98B constructs were cloned into the pcDNA5/FRT/TO/nHASt vector (Invitrogen). The resulting FAM98B truncations contained an N-terminal HA-(StrepII)2-GFP tag ...
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bioRxiv - Immunology 2021Quote: ... cells were incubated in glucose-free media containing 5 μg/ml 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG, Thermo Fisher) and 2.5% FBS at 37 °C ...
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bioRxiv - Cell Biology 2021Quote: ... total RNA from testes (n = 4 males) was extracted by using the commercial RNAqueous®-Micro kit (Ambion, Austin, USA), according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... both human primary myogenic progenitors isolated from female donors (n=2-5 per experiment) and commercialised human primary skeletal muscle progenitors (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... PC12 cells were also transiently transfected with KOR fused to FLAG on its n terminus using Lipofectamine 2000 transfection kit (ThermoFisher). Transfected cells were imaged two-three days after transfection ...
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bioRxiv - Cell Biology 2019Quote: ... Stably transfected HEK293 cells expressing N-terminally FLAG-tagged MOR or KOR were cultured in the presence of 250 μg/ml Geneticin (Gibco). For transient DNA expression ...
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bioRxiv - Biophysics 2021Quote: ... norvegicus with a HA-tag added to the C-terminal end representing the N-terminal domain (Met 1 to Val 469) of sAC (57) was cloned into pcDNA5/FRT (Invitrogen).
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bioRxiv - Biophysics 2020Quote: The 2019-nCoV receptor-binding domain (RBD) and the N-terminal peptidase domain of human ACE2 were expressed using the Bac-to-Bac baculovirus system (Invitrogen). The 2019-nCoV RBD (residues Arg319-Phe541 ...
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bioRxiv - Immunology 2020Quote: Cells were transduced with matrix-oxGFP lentivirus encoding oxGFP13 with N-terminally fused COX4L mitochondrial matrix targeting sequence and selected with 10μg/mL blasticidin (Thermo Fisher). For experimental use ...
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bioRxiv - Microbiology 2020Quote: ... These two proteins were labeled at the cysteine using thiol-reactive BODIPY™ FL N-(2-Aminoethyl) Maleimide (Thermo Fisher). The proteins were mixed with 10-fold excess BODIPY™ FL N-(2-Aminoethyl ...
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bioRxiv - Microbiology 2020Quote: ... One-step RT-qPCR was performed with primers targeting the SARS-CoV-2 N region (BEI) using the EXPRESS One-Step Superscript qRT-PCR Kit (ThermoFisher) on an Applied Biosystems QuantStudio3 instrument ...
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bioRxiv - Developmental Biology 2021Quote: ... qRT-PCR was performed using 2x ChamQ Universal SYBR qPCR Master Mix (Vazyme, L/N TE342F9) with QuantStudio3 machine (Thermofisher). The cycle procedure was at 50.0°C for 2 min ...
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bioRxiv - Molecular Biology 2021Quote: ... and HL-60 (ATCC, CCL-240) cells were grown in RPMI 1640 medium (Invitrogen, Life technologies, cat n°61870-044). Human A-431 (epidermoid carcinoma ...
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bioRxiv - Molecular Biology 2021Quote: ... and HL-60 (ATCC, CCL-240) cells were grown in RPMI 1640 medium (Invitrogen, Life technologies, cat n°61870-044). Human A-431 (epidermoid carcinoma ...
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bioRxiv - Immunology 2021Quote: The COVID-19 receptor-binding domain (RBD) and the N-terminal peptidase domain of human ACE2 were expressed using HEK293F cells (Invitrogen). The COVID-19 RBD (residues Arg319-Phe541 ...
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bioRxiv - Microbiology 2021Quote: ... the DNA sequence encoding the Nmono (monomeric N mutant K170A/R185A) was cloned by in vitro recombination (Gateway technology; Invitrogen) from pDONR207 into the yeast two-hybrid vector pPC97-GW for expression in fusion downstream of the GAL4 DNA-binding domain (GAL4-BD) ...
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bioRxiv - Physiology 2020Quote: ... 1 mM fluorescent glucose analog 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG, Life Technologies) was diluted in Live Cell Imaging Solution (Invitrogen ...
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bioRxiv - Biochemistry 2022Quote: The T7 gp5.9 gene from phage T7 was produced as a synthetic gene construct with an N-terminal 3C-cleavable histidine tag (GeneArt, Invitrogen). This was subcloned into the pACEBAC1 (MultiBac ...
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bioRxiv - Cell Biology 2022Quote: ... at 180V on precast either NuPAGE Novex 4-12% Bis-Tris Gels in 3-(N-morpholino)propanesulfonic acid (MOPS) buffer (Invitrogen), or NuPAGE™ 3-8% Tris-Acetate gels in NuPAGE™ Tris-Acetate SDS Running Buffer (Invitrogen ...
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bioRxiv - Immunology 2022Quote: ... The Hc gene with a N-terminal gp67 signal peptide and Lc gene with a N-terminal honeybee melittin signal peptide were subcloned into pFastBac Dual vector (Invitrogen) containing two multiple cloning sites ...
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bioRxiv - Physiology 2022Quote: ... and clinical CRS-isolate strain P11006 (obtained from Drs. N. Cohen and L. Chandler, Philadelphia VA Medical Center) (72) were grown in LB media (Gibco). For anti-bacterial assays ...
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bioRxiv - Neuroscience 2023Quote: Cultured neurons were incubated for 1 min with fluorescent dye N-(3-triethylammonium-propyl)-4-(6-(4-diethylamino)phenyl)-hexatrienyl)pyridinium dibromide (FM4-64; 4 µM; Invitrogen) and loaded by stimulation (10 Hz ...
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bioRxiv - Neuroscience 2022Quote: ... Supernatants were removed and spiked with 50 mM N-ethylmalamide before being added to 50 μL of magnetic protein G Dynabeads (Invitrogen) pre-conjugated with 10 μg mouse HA antibody (in house ...
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bioRxiv - Neuroscience 2022Quote: ... 20 three months old transgenic TgN3R182C150 mice were immunized initially either with 25 µg of aggregated NOTCH3 EGF1-5 protein (n=11, vaccinated) plus adjuvant (Imject Alum Adjuvant, ThermoFisher) or PBS (n=9 ...
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bioRxiv - Neuroscience 2022Quote: Total RNA was extracted from hiPSC-derived cortical neurons at DIV8 (Q5, Q6, n=3 each group) using the miRVana miRNA isolation kit (#AM1560, Ambion, Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... Maturation medium I was DMEM/F12 supplemented with 100 U/ml Penicillin-Streptomycin and 1% N-2 Supplement (Gibco, 17502048). On day 10 ...
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bioRxiv - Plant Biology 2022Quote: ... Total leaf N concentration was determined with an Organic Elemental Analyzer (Flash EA 112, Thermo Fisher Scientific Inc., MA, USA). Ash concentration was determined gravimetrically after combustion of duplicated samples for at least 4 h at 500 °C ...
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bioRxiv - Cancer Biology 2022Quote: ... The identified N-glycopeptides were quantified using the ‘Report Ion Quantifier’ available as a node in Proteome Discoverer v2.2 (Thermo Scientific) and the reporter ion intensities from the MS/MS scans were extracted from the QuantSpectra table (22) ...
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bioRxiv - Genetics 2024Quote: ... Wild type N/TERT-2G cell line was cultured using EpiLife medium with 60 µM calcium (Gibco™, cat. #MEPI500CA), supplemented with human keratinocyte growth supplement (HKGS ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNA was synthetized from 2ug of total RNA using MMLV-Reverse transcriptase and d(N)6 random primers in a 20ul reaction following the manufacturer’s protocol (ThermoFisher Scientific). RT-qPCR analyses were performed in triplicate using 2.5 μl of cDNA ...
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bioRxiv - Microbiology 2024Quote: ... at physiological pH before being spun down and resuspended in PBS with the addition of FM 1-43 Dye (N-(3-Triethylammoniumpropyl)-4-(4-(Dibutylamino) Styryl) Pyridinium Dibromide (Cat. no. T3163, Invitrogen). Images were taken on a Zeiss X10 light microscope and cell area was measured using CellProfiler 4.2.1 (33,34).
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bioRxiv - Cell Biology 2024Quote: ... and pCRSIPR-Lenti-V2 by electroporation using the NEON system (Thermo Fisher, buffer N, 4 pulses, 10 ms, 1230 V). After electroporation ...
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bioRxiv - Neuroscience 2024Quote: ... NPC were plated on poly- L-ornithine/laminin-coated flasks at 50’000 cells/cm2 in2µg/ml laminin-supplemented neural expansion medium (DMEM/F-12 + Glutamax, Gibco®; 1x N-2 supplement, Gibco® ...
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bioRxiv - Microbiology 2024Quote: O/N cultures were diluted 10000-fold in BHI and 150 μl inoculated into a round bottom 96-well plate (Nunc) to initiate biofilm formation in microtiter plates ...
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bioRxiv - Microbiology 2024Quote: ... HEK293FT (293T cells optimized for VLP production, gift from N. Manel) and OMK cells were cultivated in media DMEM (Thermofisher) containing 10% fetal bovine serum (FBS ...