Labshake search
Citations for Thermo Fisher :
1601 - 1650 of 1859 citations for N Acetyl Tizanidine d4 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2020Quote: ... we introduced a stop codon in the pLRB302 plasmid at residue 640 of the envelope ORF (C640Stop) located at the N’ terminal of the cytosolic tail using the Phusion Site-Directed Mutagenesis Kit (Thermo Fisher Scientific) and the following primers ...
-
bioRxiv - Immunology 2020Quote: ... FcMBL was biotinylated at the N terminus of the Fc protein using an N-terminal amino-oxy reaction and then coupled to streptavidin superparamagnetic beads (1µM MyOne Dynabead (Thermo Fisher Scientific, USA). Samples were screened using 5 µg of the FcMBL beads ...
-
bioRxiv - Microbiology 2021Quote: SARS-CoV-2 RBD WT for SPR binding assays (with N-terminal signal peptide and C-terminal thrombin cleavage site-TwinStrep-8xHis-tag) was expressed in Expi293F (Thermo Fisher Scientific) cells at 37°C and 8% CO2 ...
-
bioRxiv - Plant Biology 2021Quote: ... Immunoblotting of MSD2-mCherry was performed after native-PAGE or SDS-PAGE (12.5% separating gel and 4% stacking gel) with anti-mCherry primary antibody (Invitrogen, cat. n. M11217) diluted to 1:5,000 in Tris-buffered saline with 0.1% (v/v ...
-
bioRxiv - Immunology 2021Quote: ... NC_045512.2 with N-terminal signal peptide and C-terminal thrombin cleavage site-TwinStrep-8xHis-tag) were expressed in Expi293F (Thermo Fisher Scientific) cells at 37°C and 8% CO2 ...
-
bioRxiv - Microbiology 2020Quote: ... Tryptic peptides (2 μg) were loaded onto a C18 trap (75 um x 2 cm; Acclaim PepMap 100, P/N 164946; Thermo Fisher Scientific) at a flow rate of 2 ul/min of solvent A (0.1% FA and 3% acetonitrile in LC-MS grade water) ...
-
bioRxiv - Microbiology 2020Quote: ... at a flow rate of 0.3 μL/min and separated on a C18 analytical column (75 um x 50 cm; PepMap RSLC C18; P/N ES803; Thermo Fisher Scientific).
-
bioRxiv - Plant Biology 2021Quote: The pCR8-ISR1 construct was used to subclone the gene into the pMDC32 (35S cauliflower mosaic virus [CaMV] promoter) or pEarleyGate103 (35S CaMV promoter with an N terminal GFP fusion) vectors using Gateway LR Clonase II (Thermo Fisher Scientific) and then stably expressed into isr1-1 and isr1-2 plants following Agrobacterium tumefaciens (strain GV301)-mediated transformation using the floral dip method (Clough and Bent ...
-
bioRxiv - Neuroscience 2021Quote: ... The barcode sequencing libraries were quantified using a Qubit 2.0 with a Qubit™ dsDNA HS Assay Kit (Invitrogen P/N Q32854) and the quality of the libraries were performed on a 2100 Bioanalyzer from Agilent using an Agilent High Sensitivity DNA kit (Agilent P/N 5067-4626) ...
-
bioRxiv - Biochemistry 2021Quote: ... 0.03% NP40 supplemented with 50,100 or 200 mM KCl) and proteins were eluted by incubation in 1x LDS-sample buffer (Thermofisher, c/n NP0007) at 65°C for 10 minutes ...
-
bioRxiv - Microbiology 2022Quote: SpRecAA488 was made by covalently modifying primary amines (lysines or N-ter) of the protein with Alexa 488-succinidimyl ester (Molecular Probes, ThermoFisher), in presence of an excess of ssDNA (M13 mp18 ...
-
bioRxiv - Microbiology 2022Quote: APEX2 coding sequence (56) with the FLAG epitope at the N-terminus optimized for expression in human cells was synthesized by Invitrogen (GeneArt service). For the transient expression ...
-
bioRxiv - Neuroscience 2022Quote: ... iAstrocyte differentiation was initiated by switching the medium to astrocyte medium (DMEM/F12+GlutaMAX, 1% N-2 supplement, 1% fetal bovine serum, all from Thermo Fisher Scientific) as previously described [81] and continued for at least 60 days ...
-
bioRxiv - Cell Biology 2022Quote: ... transgenes were recombined with the Drosophila Gateway Vector Collection (DGVC) destination vectors expressing the N terminal tag fused target proteins under Actin5C promoter (Invitrogen, Carlsbad, CA). In addition ...
-
bioRxiv - Cell Biology 2019Quote: The pellets (n = 5/time point/group) were incubation at 48-well plate in serum-free DMEM medium 200 μl alamarBlue (Invitrogen, California, USA) 20 μl ...
-
bioRxiv - Genomics 2019Quote: ... and wild type (n = 9) mice were determined using a Countess Automated Cell Counter according to manufacturer’s protocol (Life Technologies, Carlsbad, CA). For the Tug1rescue experiment ...
-
bioRxiv - Molecular Biology 2020Quote: ... linker oligos with a 5’-terminal amine modification were obtained from IDT (Coralville, Iowa) and activated using sulfo-SMCC (Sulfosuccinimidyl-4-(N-maleimidomethyl)cyclohexane-1-carboxylate) reagent (Thermo Fisher, #A39268) according to manufacturer’s instructions ...
-
bioRxiv - Microbiology 2021Quote: ... surface lysines on MS2 capsids were modified with fluorescent dyes (NHS-Atto488 or NHS-Atto633) and the heterobifunctional maleimide-PEG24-N-hydroxysuccinimide ester crosslinker (Thermo Fisher Scientific). Second ...
-
bioRxiv - Systems Biology 2019Quote: ... with 5’-amino modification and were coupled with NHS (N-hydroxysuccinimide)-ester labelled fluorescent dyes (Alexa 488, 594, 647 (Thermo Fisher Scientific) and Cy3B (GE Healthcare) ...
-
bioRxiv - Cancer Biology 2021Quote: ... RNA from each sample was used to generate amplified and biotinylated sense-strand cDNA from the entire expressed genome according to the Sensation Plus FFPE Amplification and WT Labeling Kit (P/N 703089, Rev.4 Thermo Fisher Scientific Inc., Life Technologies). GeneChip ST Arrays (GeneChip Human Gene 2.1 ST Array Plate ...
-
bioRxiv - Bioengineering 2020Quote: ... 30 and 60 min after induction of neutralization of Col 1 and enzymatic gelation (n=2-5 samples/group) of freshly prepared materials with a Multiskan™ GO Microplate Spectrometer (Thermo Scientific). The following solutions were analyzed ...
-
bioRxiv - Bioengineering 2021Quote: ... The primate ovaries were dissected into approximately 60 fragments measuring 1×1×1mm (1 mm3) and transferred into maintenance media (α- MEM, P/N 32561, Gibco, USA) prior to encapsulation (Figure 4) ...
-
bioRxiv - Cell Biology 2021Quote: ... and anti-TRIP4 antibodies were raised in rabbits against affinity purified N-terminally hexahistidine-tagged full-length proteins and purified with the respective antigens coupled to SulfoLink resin (Thermo Fisher Scientific).
-
bioRxiv - Neuroscience 2021Quote: Rats (N = 4) were infused unilaterally with AlexaFluor-594 conjugated cholera toxin B (CTb; 5 μg/μL; Life Technologies, San Diego, CA) into the mOFC (AP +4.1 ...
-
bioRxiv - Cell Biology 2019Quote: ... GM2610 and AICS-0023 clone 20) were maintained under feeder-free condition using Essential 8 (E8) medium and vitronectin (VTN-N) (Thermo Fisher Scientific). In some cloning experiments ...
-
bioRxiv - Biochemistry 2021Quote: ... The N gene plus the upstream T7 promoter region was PCR-amplified from pSG220 using Phusion™ High-Fidelity DNA polymerase (Thermo Scientific) and standard primers ACYCDuetUP1 and DuetDOWN1 ...
-
bioRxiv - Systems Biology 2021Quote: ... The UHPLC was equipped with a trap column (Acclaim PepMap 100, 75 μm x 2 cm, C18, P/N 164535, Thermo Fisher Scientific) and a 50 cm analytical column (Acclaim PepMap 100 ...
-
bioRxiv - Biophysics 2021Quote: ... This step was performed in order to break the existing cadherin junctions and allow the blocking antibodies (anti N-Cadherin antibody: LEAF™ Purified anti-human CD325, #350804, Biolegend, USA; anti E-Cadherin antibody: CD324 #16-3249-82, Invitrogen, USA) to bind to their respective epitopes ...
-
bioRxiv - Molecular Biology 2021Quote: ... Genes were cloned into modified pRSF1-Duet expression vector with a TEV-cleavable N-terminal 14-histidine tag (39) using New England Biolabs and Anza (Thermo Fisher Scientific) enzymes ...
-
bioRxiv - Microbiology 2021Quote: ... diluted 1:1000 in PBS for 15 m at RT and mounted on glass microscope slides (Fisher Scientific, cat n. 1157-2203) using VectaShield Vibrant mounting reagent (2B Scientific).
-
bioRxiv - Immunology 2020Quote: ... blood was collected postmortem via heart puncture on day two after infection (n = 3 mice/treatment) and kept at −20 °C in RNAlater (Thermo Fisher Scientific). RNA was extracted using the Mouse RiboPure Blood RNA Isolation Kit and globin RNA depleted using the GLOBINclear Kit ...
-
bioRxiv - Biochemistry 2021Quote: SARS-CoV-2 RBD proteins for SPR binding assays (residues 328-531 of S protein from GenBank NC_045512.2 with N-terminal signal peptide and C-terminal thrombin cleavage site-TwinStrep-8xHis-tag) were expressed in Expi293F (Thermo Fisher Scientific) cells at 37°C and 8% CO2 ...
-
bioRxiv - Immunology 2021Quote: ... and used to amplify cDNA corresponding to the N and C termini of the spike protein and the plasmid backbone using Platinum SuperFi II DNA Polymerase (Thermofisher Cat# 12368010) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2021Quote: ... and used to amplify cDNA corresponding to the N and C termini of the spike protein and the plasmid backbone using Platinum SuperFi II DNA Polymerase (Thermofisher Cat# 12368010) according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2020Quote: Tumor samples with sufficient high-quality RNA (n=5) were analyzed on the Affymetrix GeneChip Human Genome U133 Plus (v.2.0) Array (Affymetrix, Santa Clara, USA) at the Microarray Department of the University of Amsterdam ...
-
bioRxiv - Cell Biology 2021Quote: ... co-cultures were fixed in 4% PFA for 30 min and immunostained for the anti-N (protocol described above) and with HCS Cell Mask TM Blue Stain (Invitrogen, 1:300). Images were acquired on an LSM 700 confocal microscope (Zeiss ...
-
bioRxiv - Developmental Biology 2022Quote: ... or 6 dpf zebrafish from one linc-mipep-/- heterozygous incross (for single-cell Multiome, n=12 per sample) in ice-cold Neurobasal media supplemented with B-27 (Thermo Fisher Scientific), snap-frozen in a dry ice/methylbutane bath (to preserve nuclear structure) ...
-
bioRxiv - Biochemistry 2022Quote: N-terminal amino acid sequencing for the eOD-GT8 60mer was performed using the Procise Protein Sequencing System (Applied Biosystems, CA, USA). The protein band corresponding to the eOD-GT8 60mer (~50 kDa ...
-
bioRxiv - Cancer Biology 2022Quote: ... cells were washed twice with PBS and overlaid with 200 μL of alkylation buffer (40 mM HEPES, 50 mM NaCl, 1 mM EGTA, protease inhibitors) supplemented with 25 mM N-ethylmaleimide (NEM; Fisher Scientific, AA4052606). Cells were incubated for 10 minutes at room temperature and then 20 μL of 10% CHAPS detergent (Sigma-Aldrich ...
-
bioRxiv - Cell Biology 2022Quote: Banked adipose-derived stem cells (ADSCs) were purchased from CellTex Therapeutics (CellTex, catalog # N/A) and cultured in growth media containing DMEM-F:12 (Gibco, catalog # 11320033) with 10% Fetal Bovine Serum (FBS ...
-
bioRxiv - Molecular Biology 2022Quote: Global proteomics for all comparative method testing was carried out (n=3 per group) on a Fusion Lumos Tribrid mass spectrometer (Thermo Fisher Scientific) coupled to an EASY-nLC 1200 (Thermo Fisher Scientific ...
-
bioRxiv - Plant Biology 2022Quote: ... Subsamples of 1000–2000 μg were carefully weighed in tin capsules to determine the total N percentage (N% as mg (100 mg DW)−1) and the 15N abundance using a FLASH 2000 Organic Elemental Analyzer (Thermo Fisher Scientific) coupled to a Delta V Advantage isotope ratio mass spectrometer (Thermo Fisher Scientific) ...
-
bioRxiv - Biochemistry 2024Quote: ... Dried samples were redissolved with 300 μL of 0.1% TFA in H2O and further fractionated using high-pH reversed-phase peptide fractionation kits (ThermoFisher, P/N 84868) following the manufacturer’s protocol ...
-
bioRxiv - Neuroscience 2022Quote: ... One-step real-time RT-PCR assays targeting SARS-CoV-2 nucleocapsid (N) coding region and subgenomic RNA were run on ABI 7900HT Sequence Detection Systems (Thermo Fisher Scientific), as previously reported25.
-
bioRxiv - Neuroscience 2022Quote: LC-MS/MS analyses of purified and desalted peptides were performed on a Dionex UltiMate 3000 n-RSLC system (Thermo Fisher Scientific), connected to an Orbitrap FusionTM TribridTM mass spectrometer (Thermo Fisher Scientific) ...
-
bioRxiv - Molecular Biology 2022Quote: COCs were collected and analyzed after 24 h of IVM by means of ROS content (n=60; minimum of 20 per group) with CellROX® Green Reagent (CRG) (Thermo Fisher) and MMP (n=60 ...
-
bioRxiv - Molecular Biology 2022Quote: Keratinocytes were transfected with either HOXC13-AS siRNA or control siRNA for 24 hours (n=3 per group) and transcriptomic profiling was performed using human Clariom™ S assays (ThermoFisher Scientific) at the core facility for Bioinformatics and Expression Analysis (BEA ...
-
bioRxiv - Neuroscience 2024Quote: ... Samples were first concentrated over an Acclaim PepMap trap column (5.0 µm C18-coated particles, 0.5 cm * 300 µm ID, ThermoFisher Scientific P/N 160454), then bound onto a PepSep XTREME column (1.5 µm C18-coated particles ...
-
bioRxiv - Bioengineering 2024Quote: ... which in turns intercalates with nucleic acids and emits red fluorescence detectable by microscopy.38 Frozen tissue sections from ATA samples (n=4 per group) were stained with DHE (10µM; D1168, Invitrogen; Carlsbad, CA, USA) in a dark humidified incubator at 37◦C for 20 minutes ...
-
bioRxiv - Biochemistry 2024Quote: ... hamster (Syrian Hamster Isoform X1 – UniProt: A0A1U8BWQ2) and macaque (Macaque – UniProt: F6SVR2) with an N-terminal cMYC-tag were ordered to GeneArt (Thermo Fisher Scientific) and cloned into a phCMV backbone (GeneBank ...