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Citations for Thermo Fisher :
1201 - 1250 of 1859 citations for N Acetyl Tizanidine d4 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2024Quote: Genomic DNA of all ancestral (A) and evolved (N) lines were isolated using Yeast DNA Extraction kit (Thermo Scientific) following manufacturer’s protocol ...
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bioRxiv - Biochemistry 2024Quote: ... or SK-N-BE(2) cells were transfected with the siRNAs using Lipofectamine RNAiMAX Transfection Reagent (ThermoFisher Scientific, #13778030) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: Frozen samples (n=8) were OCT embedded and sectioned at 10μm on a Cryostar NX70 cryostat (Thermo Fisher Scientific). Capture sections were fixed with methanol ...
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bioRxiv - Neuroscience 2023Quote: Human tissue blocks (N = 6) were stored at −80 °C and transferred to the cryostat (CryoStar NX70, Thermo Scientific) on dry ice ...
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bioRxiv - Neuroscience 2023Quote: ... and 1% n-Dodecyl-β-D-maltoside were loaded onto Novex 4-16% Bis/Tris gradient gels (Life Technologies). When assaying stoichiometry of AMPAR-auxiliary subunit complexes ...
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bioRxiv - Molecular Biology 2022Quote: ... A549 stable cell lines expressing various HCoV N-EGFP were maintained in full F-12K culture medium with 1.5μg/mL puromycin (Gibco A1113803). Cells were maintained at 37°C and 5% CO2 in a humidified environment and subcultured twice a week by DPBS washing (Gibco 14190250 ...
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bioRxiv - Molecular Biology 2023Quote: ... The cloned sequence was verified by sequencing and it was transferred to the pcDNA5-FRT-TO-N-GFP Gateway destination vector by LR recombination according to the manufacturer’s protocol (Invitrogen). HeLa Flp-In/T-REx cells ...
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bioRxiv - Microbiology 2023Quote: ... Total organic carbon (TOC) and TN were determined using a C/N analyzer (Flash EA 1112, Thermo Fisher Scientific). Soil texture was determined using the hydrometer method based on Stokes’ law (analyzed by Dr ...
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bioRxiv - Pathology 2023Quote: Primary human lung fibroblasts (n = 6 for both control and IPF, all at passage 5) were harvested using 0.25% Trypsin-EDTA (Gibco) and centrifuged at 500 x g for 5 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were maintained in serum-free neurobasal media supplemented with N-2 and B-27 Plus Supplements (Gibco™), 3 mM Gibco® GlutaMAX™ Supplement ...
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ANGPTL8 R59W variant influences inflammation through modulating NF-κB pathway under TNFα stimulationbioRxiv - Biochemistry 2023Quote: ... and was quantified using Quant-iT™ PicoGreen® dsDNA Assay Kit (Life Technologies, Grand Island,N NY, USA) and Epoch Microplate Spectrophotometer (BioTek Instruments) ...
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bioRxiv - Cell Biology 2023Quote: ... and pcDNA-DEST53 (for N-terminal GFP fusion in mammalian cells, cat#12288015, Thermo Fisher Scientific, Waltham, MA, USA). Modified forms of Ctb (Ctb-ARVA (771-FRVP-774 to 771-ARVA-774) ...
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bioRxiv - Bioengineering 2023Quote: Approximately 2×104 SK-N-BE2 or SH-SY5Y NB cells in DMEM:F12 or DMEM-high glucose medium containing 10% FBS (Gibco), respectively were seeded into a well of 96-well plate overnight ...
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bioRxiv - Cancer Biology 2022Quote: Total RNA was collected from EO771.LMB tumors that were xenografted into B6 and PWD.B6 mice (n = 4 per group) isolated using TRIzol Reagent (ThermoFisher), poly-A purified ...
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bioRxiv - Neuroscience 2023Quote: ... The sections were immunostained by primary antibodies like neuronal nuclear protein (Neu-N) (Invitrogen, California, USA, 1:500 dilution) (Gusel’Nikova et al. ...
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Engineered autocrine signaling eliminates muscle cell FGF2 requirements for cultured meat productionbioRxiv - Bioengineering 2023Quote: ... and seeded onto new 6-well plates at 2,500 cells/cm2 with 1.5 μg/cm2 of truncated recombinant human vitronectin (Vtn-N; ThermoFisher #A14700). After allowing cells to adhere overnight ...
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bioRxiv - Cell Biology 2023Quote: ... and plaque-derived sEVs (n=6) were mixed with NuPAGE lithium dodecyl sulfate sample buffer (NP0007, Thermo Fisher Scientific) at a 4:1 ratio and NuPAGE sample reducing agent (NP0009 ...
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bioRxiv - Neuroscience 2023Quote: Whole mouse brains were homogenized with a Dounce tissue grinder in neuronal protein extraction reagent (N-PER) (Thermo Scientific) containing protease/phosphatase inhibitors (Cell Signaling ...
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bioRxiv - Molecular Biology 2023Quote: ... N-2 media was replaced by B-27 media consisting of B-27 Serum-Free Supplement (Thermo Fisher Scientific), GlutaMAX (Life Technologies) ...
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bioRxiv - Microbiology 2023Quote: ... the sections were incubated with SARS-CoV-2 antibody against the nucleocapsid (N) protein (ThermoFisher MA536086; 1:28,000 dilution) for 15min at room temperature ...
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bioRxiv - Evolutionary Biology 2023Quote: We individually subcloned HRR25 orthologs to pKB11 (pDEST-DHFR F[1,2] C-term NatR) and pDN0605 (pDEST-DHFR F[1,2] N-term NatR) by LR cloning (Invitrogen, 11791), following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... the embryos were incubated O/N with an Alexa Fluor 488 anti-mouse IgG secondary antibody (Life Technologies, A28175) diluted at a final concentration of 1/200 respectively in 1% blocking solution ...
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bioRxiv - Cell Biology 2023Quote: ... and the single strand cDNA was cleaned up with DynaBeads® MyOneTM Silane Beads (Life Technologies P/N 37002D). cDNA was amplified using a Bio-Rad PTC-200 Thermal cycler with 0.2 ml 8-strip nonFlex PCR tubes ...
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bioRxiv - Biochemistry 2023Quote: ... 10x serial dilutions were made in 0.1% formic acid 0.1% n-Dodecyl-beta-Maltoside Detergent (DDM, Thermo Fisher, 89902) in LCMS grade water ...
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bioRxiv - Cell Biology 2024Quote: ... SK-N-SH and T98G were cultured in MEM/EBSS (Cytiva Life Sciences) containing 1 mM sodium pyruvate (Invitrogen), 0.1 mM NEAA (Invitrogen) ...
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bioRxiv - Biophysics 2024Quote: ... A 10 nM fluorescent bead solution was mixed with 250 µM of sulfo-NHS (N-hydroxysulfosuccinimide) (Thermo Scientific, 22980) and 250 µM of EDC (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride ...
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bioRxiv - Developmental Biology 2024Quote: ... Matrix coating was then performed by incubating the wells at room temperature for 30 min on a rocker with a mixture of 40µg/mL rhVitronectin-N (A14700, ThermoFisher) and 10µg/mL rhLaminin521 (A29249 ...
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bioRxiv - Biophysics 2024Quote: ... and α-synuclein) were mixed with an excess of N-hydroxysuccinimide (NHS)-ester-functionalized Alexa 488 dye (Thermo Fisher). The dye-to-protein ratios were optimized to maintain an average degree of labeling (DOL ...
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bioRxiv - Microbiology 2020Quote: ... the sections were sequentially incubated with mouse polyclonal antibody to SARS-CoV-2 N protein (1:500 dilution) and HRP-conjugated goat anti-mouse IgG secondary antibody (1:5000 dilution, Invitrogen). The sections were observed under microscope (Olympus ...
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bioRxiv - Cancer Biology 2021Quote: The Asparagine to Glutamine (N-Q) point mutation was performed using a Phusion SDM kit (Thermo Fisher Scientific, CAT# F541). In this assay ...
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bioRxiv - Cell Biology 2020Quote: ... The Flp-In T-Rex HEK293 cells were transfected with the N-terminal GFP-tagged FAM83F packaged in a pcDNA5-FRT/TO vector along with Flp recombinase pOG44 (Invitrogen) in a ratio of 1 μg ...
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bioRxiv - Developmental Biology 2021Quote: The Emerald Green Fluorescent Protein (EmGFP) -encoding region from Vivid ColorsTM cDNA pcDNATM6.2/N-EmGFP-DEST Vector (Thermo Fisher Scientific) was placed upstream of the coding sequence of PmlVI (human PML transcript variant 5 ...
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bioRxiv - Genetics 2021Quote: ... https://www.lifetein.com) polyclonal antisera raised against the N-terminal peptide of CenH3 [66] and anti-Rabbit-Alexa Fluor 488 (ThermoFisher Scientific). KNL2 and CENP-C were detected in root nuclei as previously described [67] using custom made anti-AtKNL2 [37] or anti-AtCENP-C antibodies (http://www.eurogentec.com/ ...
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bioRxiv - Bioengineering 2020Quote: ... Tipless MLCT-D probes (0.03 N/m spring constant) were functionalized with 10 μm diameter borosilicate glass beads (Thermo Scientific 9010 ...
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bioRxiv - Developmental Biology 2021Quote: Lymph node divested mammary glands (excluding the cervical pair) were dissected from the mice and digested O/N in DMEM/F12 (Gibco) + 10 mM HEPES (Gibco ...
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bioRxiv - Developmental Biology 2021Quote: Total RNA was isolated from the 60 hpa tissues (n=6) from each group using TRIzol reagent (Applied Biosystems, USA). One microgram of total RNA was reverse-transcribed to cDNA using a one-step cDNA Synthesis Kit (Applied Biosystems ...
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bioRxiv - Neuroscience 2022Quote: Genes encoding the full-length sequences of Human PP2A A subunit and PP2A C subunit with N-terminal His tag and non-cleavable HA tag were sub-cloned into the pFastBac-Dual vector (Invitrogen). Sequences of PP2A A and C subunits are shown in Table 1 ...
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bioRxiv - Plant Biology 2022Quote: ... AtSUMO1 was cloned into destination vector pK7WGR2 (N-terminal mRFP tag, (Karimi et al., 2002)) by LR reactions using Gateway technology (Invitrogen). Vector specific primer p35s-F used in plasmid construction are listed in Table S2.
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bioRxiv - Physiology 2022Quote: ... at 1-1.5 x 106 cells per well in William’s medium (catalog n°12551032, Gibco, Thermofisher Scientific, Waltham, MA, USA) supplemented with 5% FBS ...
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bioRxiv - Biochemistry 2022Quote: ... and 0.3 mM ATP were reacted with a 10-fold molar excess of N-(1-pyrene)iodoacetamide (Thermo Fisher Scientific) dissolved in dimethylformamide (DMF ...
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bioRxiv - Molecular Biology 2020Quote: ... of “QTARKSTGGKAPRK” related peptides (1xAcetyl [K10]; 1xGln->pyro-Glu [N-Term]; 1xmethylations [K5]) using Qual Browser (Thermo xcalibur 4.1.50, Thermo Fisher Scientific) with ±15ppm width.
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bioRxiv - Molecular Biology 2020Quote: ... at 200 V for 40 min in NuPAGE 2-(N-morpholino)ethanesulfonic acid (MES) running buffer (cat. no. NP0002, Invitrogen), supplemented with NuPAGE antioxidant (cat ...
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bioRxiv - Molecular Biology 2021Quote: Total RNA was isolated from Hela cells (n = 3) subjected to EBSS-induced autophagy and treatment with 5 μM UNC0638 for 12 hours using TRIzol reagent (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Glucose uptake was measured by the uptake of glucose analogue 2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose (2-NBDG, Life Technologies). Cell surface and intracellular cytokine stainings of splenocytes and blood lymphocytes were performed as described (42) ...
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bioRxiv - Genomics 2020Quote: ... The qPCRs were run of both technical and biological replicates (n=3) using an ABI7500 sequence detection system (Applied Biosystems by Life Technologies ...
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bioRxiv - Genomics 2020Quote: ... a puromycin N-acetyltransferase and the WPRE next to stuffer sequences was ordered as a GeneArt Synthetic Gene (Life Technologies) and PCR-amplified ...
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bioRxiv - Biochemistry 2019Quote: ... pET151/D-TOPO plasmids with the sequences of interest inserted under control of Lac operon and T7 viral promoter with N-terminal hexa-histidine tag and Ampicillin selection marker was obtained from Invitrogen. Sequences were optimized for expression in E ...
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bioRxiv - Molecular Biology 2019Quote: ... The sequence of RVFV IGR and flanking nucleotides of the N and NSs genes were synthesized by GeneArt (Thermo Fisher) for stepwise assembly of the complete minigenome in the pX12ΔT vector ...
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bioRxiv - Neuroscience 2019Quote: ... post-axotomy cultures were loaded with lipophilic dye N-(3-trimethylammoniumpropyl) -4-(6-(4-(diethylamino) phenyl) hexatrienyl)pyridinium dibromide (FM 5–95; Invitrogen) using KCl mediated depolarization as described previously (Taylor et al ...
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bioRxiv - Biophysics 2020Quote: A full-length cDNA encoding the methyltransferase SUV39H1 was amplified by PCR and fused with an N-terminal His6-MBP tag in pET-Duet1 (Invitrogen).