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Citations for Thermo Fisher :
1551 - 1600 of 1859 citations for N Acetyl Tizanidine d4 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... filled with 100 μl of water and 20 μl of 3 N aqueous sodium acetate and purified by chloroform/phenol (Life Technologies) extractions ...
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bioRxiv - Biophysics 2024Quote: Vero cells (ATCC CCL-81) were cultured in 35 mm confocal dishes (D35C4-20-1-N) with DMEM (Gibco, Carlsbad, CA) supplemented with 5% FBS (Gibco ...
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bioRxiv - Cell Biology 2024Quote: ... HT-1080 cells with TXNRD2-v5 over-expression was lysed with NP-40 buffer with 25 mM N-ethylmaleimide (NEM, 23030, Thermo Fisher) and protease inhibitor (04693116001 ...
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bioRxiv - Genomics 2024Quote: ... along with the N-terminal peptidase domain of human ACE2 was expressed using the Bac-to-Bac® baculovirus system (Invitrogen). The RBD (Residues Arg319-Phe541 ...
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bioRxiv - Microbiology 2024Quote: ... LC analysis was performed using a Kinetex 1.7 µm C8 100 Å, 50 x 2.1 mm column (S/N H17-371701, B/V 5606-0131) on a Vanquish HPLC (Thermo Scientific). The column temperature was set to 40 °C ...
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bioRxiv - Neuroscience 2024Quote: Total RNA was extracted from the ACC (n = 51) and homogenized in 350μL of lysis buffer according to the PureLink™ RNA Mini Kit (Thermo Scientific, product no ...
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bioRxiv - Microbiology 2024Quote: ... N-terminal FLAG-tagged CBP/p300 cDNA was amplified by PCR and was then inserted into pcDNA3.1 (Invitrogen Thermo, Carlsbad, CA).
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bioRxiv - Cancer Biology 2021Quote: Glucose uptake experiments were performed using 2-NBDG (2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose) (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... composed of aliquots of each EV sample from the discovery cohort (n=22 individuals) was desalted with Pierce desalting spin columns (Thermo Fisher Scientific) according to manufacturer’s guidelines ...
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bioRxiv - Cell Biology 2020Quote: ... Transfection was carried out in antibiotic-free culturing media (6ml) using 10μl lipofectamine (Lipofectamine ® 2000Reagent, 1mg/ml, Invitrogen P/N 2887) in 400μl Opti-mem media (Gibco Opti-mem reduced serum media ...
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bioRxiv - Cell Biology 2020Quote: HEK293T cells stably expressing a single copy of N-terminally tagged TTBK2- or GFP-BirA* were generated using Flp-IN T-REx cells (ThermoFisher K6500-01), Positive clones were selected with hygromycin resistance ...
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bioRxiv - Cell Biology 2020Quote: ... labelled with the fluorescent lipid dye BODIPY® FL Maleimide [BODIPY® FL N-(2-Aminoethyl) Maleimide] (Thermo Fisher Scientific; 1:200) and incubated at room temperature in the dark 10 minutes prior to analysis ...
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bioRxiv - Immunology 2021Quote: ... to target the N1 amplicon of the N gene along with TaqPath 1-Step RT-qPCR Master Mix (Applied Biosystems Waltham, MA). For the subgenomic assay ...
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bioRxiv - Genetics 2021Quote: The coding sequence for the pH insensitive Citrine fluorescent protein was inserted in the coding sequence of the BK2L3 protein (Zm00001d034049) immediately after the N-terminal secretory peptide domain and synthesized in the pDONOR221 vector (Life Technologies, USA) (Tian et al. ...
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bioRxiv - Developmental Biology 2021Quote: Protein was extracted from approximately 50mg of placental Jz tissue (n = 7 per genotype/sex, across 9 litters) using RIPA buffer (Thermo Scientific, US) containing cOmplete Mini EDTA-free protease inhibitor cocktail mix (Roche ...
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bioRxiv - Developmental Biology 2022Quote: ... GEMs were broken and the single-strand cDNA was cleaned up with DynaBeads MyOne Silane Beads (Thermo Fisher Scientific; P/N 37002D). cDNA was amplified using the C1000 Touch Thermal cycler with 96-DeepWell Reaction Module ...
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bioRxiv - Neuroscience 2021Quote: ... and for snRNA-seq the final resuspension occurred in N-PBS (PBS, 5% BSA, 5μg/mL actinomycinD and 0.2U/μl RNAse inhibitor (Thermo Fisher Scientific, Cat#N8080119)).
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bioRxiv - Neuroscience 2021Quote: ... Day 14 control and CLN5i neurons were used to isolate total cell lysates using N-PER Neuronal Protein Extraction Reagent (ThermoFisher Scientific, 87792), following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... liquid chromatography was performed with nanoUHPLC Easy nLC 1000 (Thermo Scientific brand, model EASY-nLC 1000, Easy-Spray ColumnPepMap RSLC, P/N ES801) (Thermo Scientific) coupled to a mass spectrometer with Orbitrap technology ...
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bioRxiv - Molecular Biology 2021Quote: ... The HAP1 wild type and CRISPR/Cas9 edited human knockout cell lines obtained from Horizon Genomics GmbH (Vienna, Austria) were grown in Iscove’s Modified Dulbecco’s Medium (IMDM) (Invitrogen, cat n°31980-048). More specifically ...
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bioRxiv - Biochemistry 2020Quote: Global proteomics for all comparative method testing was carried out (n=3 per group) on an LTQ Orbitrap Velos mass spectrometer (Thermo Fisher Scientific) coupled to an Eksigent nanoLC-2D system through a nanoelectrospray LC − MS interface ...
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bioRxiv - Molecular Biology 2019Quote: ... EJC mutant and wild-type sibling embryos (N = 25) were harvested at 21 and/or 27 hpf and lysed in 500 µl Trizol (Thermo Fisher Scientific). RNA was extracted following manufacturer standard procedures.
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bioRxiv - Cell Biology 2019Quote: Human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs) were cultured on Vitronectin (VTN-N) recombinant human protein (ThermoFisher Scientific, A14700). Culture vessels were coated with 200μL/cm2 of Vitronectin that had been diluted to 6μg/ml with PBS and incubated at 37°C for at least 1 hour ...
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bioRxiv - Developmental Biology 2019Quote: ... Total RNA was extracted from pooled embryos from heterozygote intercrosses or AB strain WT (n=20-30) using Trizol (Life Technologies, 15596). cDNA was prepared with SuperScript III/IV Reverse Transcription Kit (Life Technologies ...
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bioRxiv - Genetics 2020Quote: ... Total RNA was extracted from the left side of thorax with gynomorph-like color where siRNA was introduced and from the control region on the right side of the thorax of the same individual (N = 4 each for male and andromorphic female adults) using RNAqueous™-Micro Total RNA Isolation Kit (Thermo Fisher). cDNA was synthesized using SuperScript IV Reverse Transcriptase (Thermo Fisher ...
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bioRxiv - Developmental Biology 2019Quote: ... After tissue attachment, Dulbecco’s modified Eagle’s medium (Biological industries, cat: 01-053-1A) supplemented with N-2 Supplement (100X) (Thermo Fisher Scientific, cat: 17502001) and 1% FBS (Biological industries ...
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bioRxiv - Neuroscience 2020Quote: ... and then 12 µg from each was injected into a 75 μm × 2 cm nanoViper C18 Acclaim PepMap100 precolumn (3 μm particle size, 100 Å pore size; P/N 164705; Thermo Scientific). Peptides were separated at a flow rate of 250 nl/min and eluted from the column over a 5 hours gradient starting with 0.1% FA in ACN (5-30% ACN from 0 to 280min followed by 10min ramping up to 80% ACN ...
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bioRxiv - Bioengineering 2021Quote: ... and Heparin scaffold conditioned media groups (n=5) across 21 days (Days 0, 3, 7, 14, 21) using a RNAqueous™-Micro Total RNA Isolation Kit (ThermoFisher Scientific). RNA was then reverse transcribed to cDNA using a QuantiTect Reverse Transcription Kit (Qiagen ...
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bioRxiv - Plant Biology 2020Quote: ... each slide was incubated overnight at 4°C with 100 μL of rabbit polyclonal anti-β-glucuronidase antibody (N-Terminal, 5420, Molecular Probes, Invitrogen) diluted 1:200 in fresh blocking buffer (3% BSA in 1X PBS) ...
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bioRxiv - Cell Biology 2020Quote: S1R was expressed with an N-terminal 6*His-tag fusion in Sf9 cells using Bac-to-Bac baculoviral expression system (Thermo Fisher Scientific) according to manufacturer’s recommendations ...
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bioRxiv - Biochemistry 2020Quote: pcDNA5-based constructs for the expression of C-terminally His6-PreScission protease cleavage site-2xFlag (Flag)-tagged full-length or N-terminally truncated ASCC3 variants were transfected into HEK293 Flp-In™ T-REx™ cells (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2021Quote: ... U2OS cells were first transfected with PiggyBac Tet-On 3G-(ArrayG/N)16X and PBQM-mwGFP plasmids using the Neon Transfection system (Thermo Fisher Scientific) and the following pulse program (pulse voltage ...
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bioRxiv - Neuroscience 2020Quote: Hippocampi removed from euthanized 8-month-old 5×FAD mice previously bilaterally injected with saline or N-Aβcore were homogenized with 200-250 μL of Pierce I.P Lysis Buffer (ThermoFisher Scientific, # 87788, lot# MJ162614) with 1x Halt Protease and Phosphatase Inhibitor Cocktail (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... and anti-N-WASP (Cell Signalling #4848S; 1:100) followed by Alexa Fluor 488 conjugated secondary antibodies (Invitrogen; 1:1000 in PBS). Actin tails were labeled with Alexa Fluor 488 ...
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bioRxiv - Biochemistry 2021Quote: Formerly N-glycopeptides were separated by nano-LC-MS/MS coupled to an ESI-LTQ-Orbitrap Velos mass spectrometer (Thermo Fisher Scientific). Mass spectrometric analysis was performed with the following settings ...
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bioRxiv - Microbiology 2021Quote: ... sterols dissolved in chloroform were dried under nitrogen gas (N-EVAP, Organomation) followed by high vacuum for at least 1 hour (Savant SpeedVac, Thermo Fisher Scientific). They were then hydrated with 2.5 mM MβCD prepared in serum-free medium ...
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bioRxiv - Microbiology 2021Quote: ... Appropriate correction factors for the individual TMT channels for both lysine side-chain labelling and peptide N-terminal labelling as per the TMT-6plex kits used (Thermo Fisher Scientific) were configured into the database search ...
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bioRxiv - Immunology 2021Quote: ... lungs and kidneys were isolated from perfused animals and incubated over-night (O/N) in RNAlater™ stabilisation solution (Thermo Fisher Scientific) for RNA analysis ...
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bioRxiv - Plant Biology 2021Quote: ... Final constructs were obtained by transferring all candidate genes subsequently into the plant binary destination vector pAlligator2 (N-terminal triple HA fusions=HA3) (Bensmihen et al., 2004) via LR reactions (Thermo Fisher Scientific). Constructs were transformed into Agrobacteria (Rhizobium radiobacter ...
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bioRxiv - Molecular Biology 2020Quote: ... CK2A1 or PRKACA coding sequences were cloned into the destination vector pDEST-pcDNA5-BirA*-FLAG N-term with LR clonase 2 (Thermo Fisher Scientific) using the Gateway system ...
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bioRxiv - Cell Biology 2020Quote: ... was solubilized with 0.1 N NaOH and protein concentration was determined using Pierce™ BCA Protein Assay Kit (Thermo Fisher, Waltham, MA). Cellular cholesterol content data was expressed as mg cholesterol/mg of protein ...
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bioRxiv - Biophysics 2021Quote: ... was cloned into a modified pEZT-BM vector with an N-terminal Flag tag (Morales-Perez et al., 2016) and heterologously expressed in HEK293F cells using the BacMam system (Thermo Fisher Scientific). Bacmids were synthesized using E ...
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bioRxiv - Molecular Biology 2022Quote: ... If required (as indicated for the methods for the respective experiments) hiPSCs were adapted to feeder-free conditions on Vitronectin (VTN-N, Thermo Fisher Scientific) coated plates in mTeSR-plus (StemCell Technologies ...
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bioRxiv - Neuroscience 2022Quote: ... the pellet (synaptosomal fraction) was re-suspended and sonicated in N-PER Neuronal Protein Extraction Reagent (ThermoFisher Scientific, Waltham, MA, USA; 87792). Protein concentration was determined via the BCA protein assay kit (ThermoFisher Scientific ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Telomerase-immortalized human neonatal foreskin keratinocytes (N-TERTs) (21) were cultured in EpiLife medium containing human keratinocyte growth supplement (HKGS) (Thermo Fisher Scientific) and penicillin/streptomycin ...
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bioRxiv - Biophysics 2022Quote: ... and apoA1 were purified using established protocols for MSP44 with the following modifications: A synthetic construct of apoA1 bearing a 3C protease cleavable N-terminal deca-histidine tag (GeneArt/Thermo Fisher Scientific) was cloned into a pET28a vector (Addgene ...
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bioRxiv - Microbiology 2022Quote: ... a plasmid encoding the EGFP-N fusion protein was constructed and transfected into infected HEp-2 cells with Lipo3000 (Thermo Fisher, USA) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2022Quote: ... a pET21a vector including hTS with an N-terminal 6x histidine tag was transformed into BL21 (DE3) cells lacking the rne131 gene (Invitrogen, Carlsbad, CA). After an adaptation period (Bonin & Lee ...
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bioRxiv - Biophysics 2022Quote: The gene encoding N-terminal His-tagged V2HeR3 was cloned into the EcoRI and XbaI site of pPICZB vector (Thermo Fisher Scientific). The recombinant protein was expressed in the Pichia pastoris strain SMD1168H (Philosof et al. ...
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bioRxiv - Biophysics 2022Quote: ... the gene encoding N-terminal His-tagged V2HeR3 was cloned into the EcoRI and XbaI site of pPICZB vector (Thermo Fisher Scientific). For determination of λmax of V2HeR3 WT and mutants ...