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Citations for Thermo Fisher :
1151 - 1200 of 1859 citations for N Acetyl Tizanidine d4 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... Tyr-nitration of MSD2 was detected after SDS-PAGE with anti-Nitrotyrosine primary antibody (Invitrogen, cat. n. A-21285) diluted to 1:3,000 in TBST with 3% (w/v ...
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bioRxiv - Cancer Biology 2020Quote: ... (2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG) (100 μM; ThermoFisher Scientific) with Hoechst (1 μg/mL ...
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bioRxiv - Cell Biology 2021Quote: ... and the N-terminus of the construct was attached to a biotinylated DNA-coated paramagnetic bead (2.8 μm diameter, Invitrogen) via a biotin-neutravidin interaction ...
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bioRxiv - Cell Biology 2021Quote: ... and 2-NDBG [2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose] (N13195) were purchased from Invitrogen. Recombinant murine SCF (250-03) ...
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bioRxiv - Biophysics 2020Quote: A1-LCD was fluorescently labeled on the N-terminus using Alexa Fluor® 488 NHS ester fluorescent dye (ThermoFisher) to label the primary amines of proteins ...
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bioRxiv - Microbiology 2022Quote: ... The pellet containing the membranes was resuspended in 20 mM phosphate buffer pH 7.4 with 0,3% n-octyl-poly-oxyethylene (OPOE) detergent (Affymetrix, UK) and incubated at 20°C for 2 h under agitation to separate inner (IM ...
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bioRxiv - Cell Biology 2022Quote: HEK 293T cells were transfected with 1 µg of plasmids containing either FL (wild-type), various deletions (ΔTM, ΔNΔTM, NCC, CC, N) or mutant FLAG-KASH5 constructs with Lipofectamine LTX (Invitrogen) transfection reagent using the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... and N-((6-(biotinoyl)amino)hexanoyl)-1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine (biotin-X DHPE; Molecular Probes, Life Technologies). Planar bilayers were formed from 200-nm liposomes in channels of a PDMS flow-cell using vesicle spreading method (36) ...
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bioRxiv - Microbiology 2022Quote: ... and N-((6-(biotinoyl)amino)hexanoyl)-1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine (biotin-X DHPE; Molecular Probes, Life Technologies). Planar bilayers were formed from 200-nm liposomes in channels of a PDMS flow-cell using vesicle spreading method (36) ...
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bioRxiv - Neuroscience 2022Quote: ... and incubated with primary antibody O/N at 4°C (5% FBS, 0.5% Tx, 0.2% gelatine in PBS; GFP (1:500, ThermoFisher Scientific). Slices were then repeatedly washed with PBS-0.5% Tx ...
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bioRxiv - Biophysics 2022Quote: The human wild-type S1PR5 gene (UniProt ID Q9H228) with an N-terminal 3 × HA epitope (YPYDVPDYA) tag was cloned into pcDNA3.1+ (Invitrogen) at KpnI(5’ ...
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bioRxiv - Plant Biology 2022Quote: ... fused with an N-terminal 6×His-tag and cloned into expression vector pPIC9 (Thermo Fisher Scientific, California, USA). Correctness of the resulting constructs was confirmed by DNA sequencing prior to introduction into Pichia pastoris strain GS115 (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... Taqman gene expression assays for N2 and N3 regions of the SARS-CoV-2 N gene were made (ThermoFisher) following CDC specifications (Division of Viral Diseases ...
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bioRxiv - Immunology 2021Quote: ... of M13KO7 helper phage and grown o/n at 37°C in fresh 2xYT medium supplemented with Amp100 and 75μg/mL of kanamycin (Kan75, Gibco) to select for bacteria containing a phagemid and infected with a helper phage ...
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bioRxiv - Molecular Biology 2020Quote: ... The peptides were separated on an in-house made C18 column (20 cm length, 75 μm inner diameter, 1.9 μm particle size) by an easy n-LC 1200 (ThermoFisher) and directly injected in a QExactive-HF or in case of TMT samples into a Fusion Lumos mass-spectrometer (ThermoFisher ...
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bioRxiv - Microbiology 2021Quote: ... N gene reverse primer (5’-GAGGAACGAGAAGAGGCTTG-3’) and probe (5’-FAM-ACTTCCTCAAGGAACAACATTGCCA-QSY-3’) using Taqman mastermix (Thermo Fisher). The thermal cycling steps were ...
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bioRxiv - Microbiology 2022Quote: ... offline high pH reverse-phase fractionation was performed using a Hypersil Gold column (Thermo Scientific, P/N 25002-202130). The mobile phase A was water adjusted with ammonium hydroxide to pH 10 and B was 10 mM ammonium bicarbonate in 80% Acetonitrile (ACN ...
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bioRxiv - Genomics 2022Quote: ... An N-terminal FLAG tag and a nuclear localization signal (NLS) along with the M.CvIPI ORF were then cloned N-terminal to Lamin B1 into pENTR (Invitrogen) using Gibson cloning (New England Biolabs) ...
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bioRxiv - Systems Biology 2022Quote: ... streptavadin magnetic beads were aliqoted and washed in 0.1 N NaOH followed by two washes in 1× SSC buffer (Invitrogen). Hybridized RNA and probes were diluted to the original bead volume using a 1 × SSC and 20% formamide solution and incubated at room temperature for 3 minutes prior to bead capture ...
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bioRxiv - Cancer Biology 2022Quote: ... 2 million SK-N-BE(2)-FLUC cells were stained immediately before injection with CellTrace™ Far Red (Invitrogen) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... The gel was then transferred to Hybond N+ (Amersham) nylon membrane and blotted with HRP-Conjugated Streptavidin (Thermo Scientific) with a dilution factor 1:2000 ...
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bioRxiv - Bioengineering 2020Quote: ... Proliferation was assessed using a Click-iT EdU Alexa Fluor 594 Imaging Kit (ThermoFisher; n=3/group/time point). EdU was added to media at a concentration of 10 µM at days 2 and 6 of culture and incubated for 24 hours before gels were fixed at days 3 and 7 ...
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bioRxiv - Genomics 2019Quote: ... two similar arrays were used for targeted genotyping within the study population: the UK BiLEVE Axiom Array (n=49,950) by Affymetrix and the UK Biobank Axiom Array (n=438,427) ...
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bioRxiv - Neuroscience 2019Quote: ... Cells were obtained from embryonic cortices (n=10-12/cell preparation) dissected in HBSS (Life Technologies, Carlsbad, CA, USA) from timed pregnant Sprague Dawley rats (Harlan Laboratories ...
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bioRxiv - Physiology 2019Quote: ... 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG, Thermo Fisher Scientific, USA) was dissolved in PBS at 5 mg/ml ...
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bioRxiv - Genetics 2021Quote: ... We dissected the facial primordia (n = 5 from each breed) and stored the tissue in RNAlater (Thermo Fisher Scientific) at −80°C ...
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bioRxiv - Biochemistry 2021Quote: ... the Lambda DNA in TE from Quant-iT ™ PicoGreen® dsDNA Assay Kit (ThermoFisher Scientific, cat. n. P11496) was used ...
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bioRxiv - Immunology 2020Quote: ... Glucose uptake assay was performed by incubating cells with 50 µM 2-NBDG (2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose) (Invitrogen) for 90 min at 37°C prior to cell staining ...
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bioRxiv - Immunology 2020Quote: The validation cohort included a collection of selected G1 and G3 ccRCC tissues (n = 14 each) and corresponding normal kidney tissue (n = 14) microarray data (“Rostock cohort”, Array GeneChip HG U133 Plus 2.0, Affymetrix) (Maruschke et al. ...
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bioRxiv - Neuroscience 2019Quote: ... The antigen peptide with N-terminus Cysteine (NH2-CGGGGAAPGPPRHG-COOH) was coupled with keyhole limpet hemocyanin (Thermo fisher 77600). The antigen was injected into rabbit and antisera were collected after primary and several booster injections ...
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bioRxiv - Genetics 2020Quote: ... Total RNA was isolated from ontogenetic and regenerating scales (n=3 fish per group, RU) by using Trizol (Invitrogen) for RNA-sequencing (RNA-seq ...
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bioRxiv - Physiology 2020Quote: ... muscles were stained for 3 minutes with 10μM 4-(4-diethylaminostyrl)-N-methylpyridinium iodide (4-Di-2ASP, Molecular Probes) to allow imaging muscle with an upright epifluorescence microscope (Leica DMR ...
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bioRxiv - Biochemistry 2021Quote: ... The construct with a N-terminal 8xHis-TwinStrep-GFP-tag were expressed in GripTite HEK293 MSR cells (Thermo Fisher) in black 96 well plates with transparent bottoms ...
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bioRxiv - Biochemistry 2021Quote: ... H3.3 was cloned into pLEX_305-C-dTAG and p53 as well as RelA were cloned into pLEX_305-N-dTAG using Gateway LR clonase II Enzyme mix kit (Invitrogen). The sequences of the primers are listed below.
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bioRxiv - Cell Biology 2021Quote: ... or 5 µM BODIPY™ FL C5-Ceramide (N-(4,4-Difluoro-5,7-Dimethyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Pentanoyl)Sphingosine) (Invitrogen, D3521) together with 5 µM delipidated BSA (as a delivery carrier ...
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bioRxiv - Cell Biology 2021Quote: ... WT and SIRT1 KO mESCs cultured in serum-free ESGRO medium or serum-containing M10 medium were incubated with 5 µM BODIPY™ FL C5-Sphingomyelin (N-(4,4-Difluoro-5,7-Dimethyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Pentanoyl)Sphingosyl Phosphocholine) (Invitrogen, D3522) or 5 µM BODIPY™ FL C5-Ceramide (N-(4,4-Difluoro-5,7-Dimethyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Pentanoyl)Sphingosine ...
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bioRxiv - Immunology 2021Quote: ... Copies of SARS-CoV-2 N were measured by qRT-PCR TaqMan Fast Virus 1-step assay (Applied Biosystems). SARS-CoV-2 specific primers and probes from the 2019-nCoV RUO Assay kit (Integrated DNA Technologies ...
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bioRxiv - Genetics 2021Quote: ... The samples were either frozen (n = 6) or stored in the RNAlater reagent (Thermo Fisher Scientific, Waltham, MA, USA) (n = 4).
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bioRxiv - Immunology 2021Quote: ... Molecular detection of SARS-CoV-2 was performed with TaqMan™ Gene Expression Assay (Cat. N. 4331182, ThermoFisher®) with specific primers/probes previously described40 ...
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bioRxiv - Genetics 2020Quote: Total RNA was isolated from gonadal adipose tissue (eWAT; n = 9) samples using the Ambion RiboPure (Thermo Fisher Scientific). RNA was quantified in a Nanodrop ND-1000 spectrophotometer and RNA purity and integrity was checked by using a Bioanalyzer-2100 equipment (Agilent Technologies ...
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bioRxiv - Biochemistry 2022Quote: ... we resuspended the frozen nuclei fractions in 300 μL of N-PER™ Neuronal Protein Extraction Reagent (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2022Quote: ... 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl) (16:0 NBD) were purchased from Invitrogen. Phosphate-buffered saline (PBS ...
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bioRxiv - Cell Biology 2022Quote: Large-scale production of bio-inactive human N-SLIT2ΔD2 was performed by transfecting HEK293F cells with human N-SLIT2ΔD2 cDNA (1 ug/mL) using PEI reagent (Polyethyleneimine, linear, M.W. 25,000, Thermo Fisher Scientific) (19) ...
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bioRxiv - Genetics 2022Quote: Our gene interaction-perturbation pipeline applied the discovery cohort (n =2,167) composed of 19 independent datasets from the same chip platform (Affymetrix Human Genome U133 Plus 2.0 Array ...
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bioRxiv - Immunology 2022Quote: ... and excess N-ethylmaleimide was removed by filtration with Zeba spin columns with a 7kDa cut-off (Thermo Fisher). Sulfenic acid residues were selectively reduced with 200 mM sodium arsenite and newly released free thiols were labeled by adding 200 mM 1mM biotin–maleimide ...
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bioRxiv - Immunology 2022Quote: ... cells were resuspended in glucose-free media containing 150 μM 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG Thermofisher) and incubated for 45 minutes at 37°C.
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bioRxiv - Cancer Biology 2024Quote: Frozen samples (n=8) were OCT embedded and sectioned at 10μm on a Cryostar NX70 cryostat (Thermo Fisher Scientific). Capture sections were fixed with methanol ...
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bioRxiv - Cell Biology 2023Quote: ... pcDNA5/FRT/TO N-YFP vector was double digested by restrictive enzymes of KpnI and NotI (Thermo Fisher Scientific). Wild type Cdc20 was amplified by PCR and inserted into the expression vector according to standard procedures ...
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bioRxiv - Biochemistry 2024Quote: ... or SK-N-BE(2) cells were transfected with the siRNAs using Lipofectamine RNAiMAX Transfection Reagent (ThermoFisher Scientific, #13778030) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... n=6) before being perfused intravenously with fluorescent Texas Red 40 kDa Dextran (3 mg/kg; D1829, Fisher Scientific) as described in previous studies (25,26 ...