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Citations for Thermo Fisher :
1251 - 1300 of 1859 citations for N Acetyl Tizanidine d4 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2019Quote: cDNA was synthesized from 1000 ng of RNA extracted from whole VNO of P21 Smad4flox/flox animals (n=3) by using the kit by Invitrogen Super ScriptIII First Strand (Cat# 18080400) ...
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bioRxiv - Cell Biology 2019Quote: ... FM™ 4-64 Dye (N-(3-Triethylammoniumpropyl)-4-(6-(4-(Diethylamino) Phenyl) Hexatrienyl) Pyridinium Dibromide) was purchased from Invitrogen. Rapamycin was purchased from LC Laboratories ...
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bioRxiv - Neuroscience 2019Quote: ... Six of the ganglia were immediately fixed in 4% paraformaldehyde (n = 3 animals) or placed in RNAlater (ThermoFisher, Cat. #AM7021) for RNA extraction.
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bioRxiv - Neuroscience 2019Quote: ... a Tet-inducible EGFP-RBM45 construct was generated by inserting the EGFP-RBM45 (N-terminal EGFP fusion) into the pcDNA4/TO vector (Invitrogen). The sequences of all constructs were confirmed by Sanger DNA sequencing and the sizes of the expressed proteins confirmed by Western blot.
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bioRxiv - Molecular Biology 2019Quote: ... The VTN-N solution was removed prior to sticking the Press-to-Seal Silicone Isolator with Adhesive (Thermo Fisher Scientific) onto the nanosheets to control the density of the cells (see “Cell cultures” in METHODS).
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bioRxiv - Immunology 2021Quote: ... virus-infected cells were detected using an in-house rabbit polyclonal antibody specific for SARS-CoV-2 N/M/E followed by horseradish peroxidase-conjugated goat anti-mouse IgG (Invitrogen). TCID50 was calculated using the Reed-Muench method.
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bioRxiv - Biochemistry 2021Quote: ... A673 (ATCC-CRL-1598) and SK-N-MC (ATCC-HTB-10) cells were grown in Dulbecco’s modified Eagle Medium (DMEM, Thermo Fisher) supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Bioengineering 2021Quote: ... decellularized apple scaffolds (prior to MC3T3-E1 cell seeding) (n=3) were thoroughly washed with phosphate buffered saline (PBS; ThermoFisher) and incubated in 1 mL of 10% (v/v ...
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bioRxiv - Bioengineering 2020Quote: ... A biphasic solvent overlay was employed to capture patchoulol by adding 7 mL of filter-sterilized n-dodecane (Acros Organics) to each culture (10% v/v) ...
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bioRxiv - Bioengineering 2020Quote: Total RNA was extracted from HTM cells ± DEX at 7 d (N = 3 per group and donor) using PureLink RNA Mini Kit (Invitrogen). RNA concentration was determined with a NanoDrop spectrophotometer (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2021Quote: ... full length N-terminally 3xFLAG tagged human KNSTRN ORF was cloned into pTREx-DEST30 vector by serial Gateway recombinations (Invitrogen) according to the manual ...
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bioRxiv - Cell Biology 2021Quote: ... TDP-43 CRISPR KO HeLa cells were lysed for N/C fractionation and SDS-PAGE with the NE-PER kit (ThermoFisher) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Live cells were imaged using an Olympus 40x UPlanFL N Oil NA1.3 objective in imaging medium (Leibovitz’s L-15 medium (Gibco™ 21083027) without phenol red ...
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bioRxiv - Systems Biology 2020Quote: ... the mouse mNCK2 cDNA sequence was cloned via restriction enzymes in frame with a N-terminal 3XFLAG tag in pcDNA3.1(-) (ThermoFisher Scientific) for western blot (WB ...
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bioRxiv - Microbiology 2021Quote: ... Streptomyces hyphae were incubated with 0.5 mg/ml FM 4-64 Dye (N-(3-Triethylammoniumpropyl)24-(6-(4-(Diethylamino) Phenyl) Hexatrienyl) Pyridinium Dibromide) (Molecular Probes) for 15 min in the dark ...
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bioRxiv - Biochemistry 2020Quote: ... were PCR-amplified and cloned into the pDEST15 vector encoding an N-terminus GST tag using the Gateway Cloning Technology (Invitrogen)27 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... the protein with an N-terminal His10 tag was expressed in Sf9 cells following the Bac-to-Bac baculovirus system (Invitrogen). Cells were collected and solubilized to extract protein in the buffer containing 25 mM MES pH 6.0 ...
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bioRxiv - Synthetic Biology 2021Quote: ... These two proteins were labeled at the cysteine using thiol-reactive BODIPY™ FL N-(2-Aminoethyl) Maleimide (Thermo Fisher). The proteins were mixed with 10-fold excess BODIPY™ FL N-(2-Aminoethyl ...
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bioRxiv - Microbiology 2020Quote: ... The plasmids were purified from 2 ml of the O/N culture using the GeneJET Plasmid Miniprep Kit (ThermoFisher Scientific), and sent for Sanger sequencing to DNA Sequencing and Genomics ...
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bioRxiv - Microbiology 2020Quote: PvRBP2b161-1454 and TfR1 were labelled with N-hydroxysuccinimide ester-activated DyLight 488 (DL488) and DyLight 594 (DL594) (Life Technologies) respectively ...
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bioRxiv - Cell Biology 2021Quote: ... M2DCTs with an MyBP-C antibody (N-terminal rabbit polyclonal, 1:1000, kind gift from Samantha Harris) and anti-rabbit AlexaFluor secondary (ThermoFisher). Intercalated disk images were obtained by labeling fixed M2DCTs with an N-cadherin antibody (1:200 ...
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bioRxiv - Cell Biology 2021Quote: ... C-term (aa 166-499)) were produced by PCR from the HA-PRPF31 plasmid and cloned into the pcDNA3.1 N-HA vector (Thermo Fisher). Deletion constructs of PRPF6 (NTD (aa 1-126) ...
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bioRxiv - Biophysics 2022Quote: ... Purified Syb2 (29–96, S61C) was labeled with 6× molar excess BODIPY FL N-(2-aminoethyl)-maleimide (BDPY) (Molecular Probes) and SN25 FL (1–206 ...
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bioRxiv - Biophysics 2022Quote: The SARS-CoV-2 Omicron RBD and the N-terminal peptidase domain of ACE2 were expressed using the Bac-to-Bac baculovirus system (Invitrogen) as previously stated ...
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bioRxiv - Systems Biology 2022Quote: ... Transcriptomics using the isolated mRNA from liver tissues (0, 2, 4, 6, 8 and 10 weeks; n=3 per time point) was performed by Affymetrix GeneChip®Mouse Gene 2.0 ST Arrays (902118) ...
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bioRxiv - Microbiology 2022Quote: ... The blocking buffer was then aspirated and 50 μL of a 1:4000 dilution of rabbit anti-SARS-CoV-2 nucleocapsid (N) antibody (MA536086, Invitrogen) in blocking buffer was added and incubated for 2 h at 37°C ...
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bioRxiv - Microbiology 2022Quote: SARS-CoV-2 Beta RBD for crystallization (residues 328-531 of S protein from GenBank NC_045512.2 with N-terminal signal peptide and C-terminal 8xHis-tag) was expressed in Expi293F (Thermo Fisher Scientific) cells in the presence of 10 µM kifunensine at 37°C and 8% CO2 ...
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bioRxiv - Neuroscience 2022Quote: ... The real-time quantitative PCR (qRT-PCR) was performed with TaqMan Fast Advanced Master mix (ThermoFisher Scientific, catalog n°4444963) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... a 6 x His tag and linker was fused to the N-terminus of the GJA1-20k sequence and cloned into the pET301/CT-DEST vector via Gateway cloning (Invitrogen). The primers used to amplify the sequence are (5’ to 3’ ...
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bioRxiv - Neuroscience 2022Quote: ... was dissolved in 0.9% sterile Saline and pH was adjusted to 7.4 with 20-30 µl 1 N NaOH (#SS266-1, Fisher Scientific) according to Tocris Bioscience’s guidelines ...
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bioRxiv - Developmental Biology 2022Quote: Ear or tail biopsies were lysed overnight (o/n) at 55°C in DirectPCR lysis reagent (BioSite) in the presence of 0.1 mg/mL Proteinase K (Thermo Scientific). Lysis was stopped by heat inactivation (45min ...
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bioRxiv - Neuroscience 2020Quote: ... and equally distributed in wells containing growth medium (DMEM-F12-glutamax [Gibco], 40 μg/mL insulin [Sigma], 1/200 B-27 supplement [Gibco], 1/100 N-2 supplement [Gibco] ...
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bioRxiv - Microbiology 2019Quote: Immunohistochemistry was performed using monoclonal antibodies directed to MV N protein (clone 83KKII, Chemicon [57]) or rabbit polyclonal antibody directed to GFP (Invitrogen). Goat anti-mouse IgG1 or goat anti-rabbit antibody conjugated with biotin was included as secondary antibody ...
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bioRxiv - Immunology 2020Quote: ... and SARS-CoV-2 and the N-terminal peptidase domain of human ACE2 (residues Ser19-Asp615) were expressed using the Bac-to-Bac baculovirus system (Invitrogen) as previously described 18,19,37-39 ...
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bioRxiv - Microbiology 2020Quote: Human IFITM proteins and SARS-CoV-2 viral proteins were cloned into MaMTH N-term tagged Prey and C-term tagged Bait vectors respectively using Gateway cloning technology (ThermoFisher). Correctness of recombined insertions was confirmed by Sanger sequencing (Eurofins) ...
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bioRxiv - Microbiology 2020Quote: Beads were labeled with the following N-hydroxysuccinimide ester (NHS)-activated fluorophores: (i) Alexa Fluor 488 NHS ester (Fisher Scientific) (ii ...
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bioRxiv - Microbiology 2021Quote: ... and the N-terminal peptidase domain of human ACE2 (residues Ser19–Asp615) were expressed using the Bac-to-Bac baculovirus system (Invitrogen) as described previously21 ...
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bioRxiv - Microbiology 2021Quote: ... The SARS-CoV-2 N standards were generated by in-vitro transcription of the generated SARS-CoV-2 N cDNA using the MegaScript T7 Transcription kit (Invitrogen), followed by using the MEGAclear Transcription Clean-Up Kit ...
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bioRxiv - Cancer Biology 2021Quote: ... NPCs were plated on Geltrex in a 6 well plate in StemPro NSC SFM supplemented with 20ng/ml of EGF and bFGF for 2 days before switching to astrocyte differentiation medium consisting of DMEM with 1% N-2 supplement (Gibco), 1% GlutaMAX-I supplement (Gibco ...
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bioRxiv - Immunology 2021Quote: ... IF staining was performed on deparaffinized and rehydrated tissue sections using rabbit anti-SARS-CoV-2-N protein antibody together with AF568-conjugated goat anti-rabbit IgG (Invitrogen). Briefly ...
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bioRxiv - Bioengineering 2021Quote: ... The ovaries were dissected into 2–4 pieces and transferred into maintenance media (α-MEM, P/N 32561, Gibco, USA) and placed in a CO2 incubator prior to encapsulation.
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bioRxiv - Neuroscience 2022Quote: Freshly prepared solubilized proteins were incubated o/n at 4°C with affinity-purified rabbit anti-P2X4 antibodies (Alomone) cross-linked to magnetic beads (Dynabeads, Invitrogen). The flow through was discarded and the beads washed 5 times with wash buffer (CL48 diluted ¼ in PBS and supplemented with complete protease inhibitor cocktail (Roche) ...
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bioRxiv - Neuroscience 2022Quote: ... Detection and relative quantification of the prevalence of individual N-glycans was accomplished using the total ion mapping (TIM) functionality of the Xcalibur software package version 2.0 (ThermoFisher Scientific) as previously described (Aoki et al. ...
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bioRxiv - Microbiology 2022Quote: ... which were subsequently purified and ligated upstream the His-tag into the expression vector pTrcHis2 (Invitrogen™, cat. n° V36520). The recombinant vectors were transformed into the ΔarsC E ...
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bioRxiv - Cell Biology 2019Quote: The pellets (n = 5/time point/group) were rinsed in PBS and lysed in 500 μl TRIzol (Invitrogen, CA, USA) for 10 min ...
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bioRxiv - Microbiology 2019Quote: ... prepared minipreps from 2 ml of O/N cultivation at 37 °C using the GeneJET Plasmid Miniprep Kit (ThermoFisher Scientific), checked for the presence of insert using restriction digest and agarose gel electrophoresis ...
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bioRxiv - Biophysics 2019Quote: ... the HEK-293T cells were transfected with a plasmid encoding full-length LRRK2(I2020T) fused with YFP at the N-terminus using a Lipofectamine 3000 transfection kit (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: The C-terminal fragments of Stu2 used in the pulldown experiments were cloned into a pLIC vectors with a TEV-cleavable N-terminal 6-His tag and transformed into Bl-21 AI cells (Invitrogen). Cells were grown to an OD600 of 0.6 in TB media with 0.1% glucose and were induced by addition of IPTG and arabinose to 200 μM and 0.2% ...
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bioRxiv - Cell Biology 2021Quote: ... WT human PRRT2 (gene ID: 112476) was synthesized as N-terminal HA tag fusion protein (Genewiz) in pcDNA3 vector (Thermofisher) and single point mutants were generated on this matrix by standard molecular biology techniques.
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bioRxiv - Biochemistry 2021Quote: ... Julien Prudent (MRC Mitochondrial Biology Unit, Cambridge, UK) and grown in Dulbecco’s Modified Eagle’s Medium (Life Technologies, cat. n. 31885023) supplemented with 10% FBS (Life Technologies ...