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Citations for Thermo Fisher :
1501 - 1550 of 1859 citations for N Acetyl Tizanidine d4 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... Cnn or TACC CDS were recombined with a destination pRNA vector containing a GFP CDS at either the N- or C-terminus using Gateway technology (ThermoFisher Scientific).
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bioRxiv - Neuroscience 2024Quote: PVN tissue biopsies were collected from fresh-frozen brain tissue and protein extracted using Neuronal Protein Extraction Reagent (N-PERTM, Thermo Scientific) for determination of CRH protein levels by Western Blot ...
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bioRxiv - Neuroscience 2024Quote: Determination of sIL-6R was performed in the FN of NL and 21 dpi of aged WT and GFAP-IL6Tg (n=3-6/experimental group) using a precoated mouse ELISA kit (EMIL6RA, Fisher Scientific) and following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... zebrafish larvae from visually similar developmental stages were collected in groups of 16 individuals from all corresponding genotypes (N = 4-5 per genotype) and stored in RNAlater (Thermo Fisher) until use ...
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bioRxiv - Neuroscience 2024Quote: ... Neuronal differentiation was induced by culturing NGN2-NPC in neural differentiation medium (DMEM/F-12 + Glutamax, Gibco®; 1x N-2 supplement, Gibco® ...
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bioRxiv - Neuroscience 2024Quote: ... The cell culture medium was removed and cells were suspended with 1 mL ice-cold PBS and then treated with 1 mL sulfo-N-hydroxysuccinimide-SS-biotin (Thermo Scientific) (1 mg/mL in PBS ...
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bioRxiv - Cell Biology 2024Quote: ... hansenii cells were concentrated 10x in 200 μL YPD containing 40 μM FM4-64 dye (N-(3-Triethylammoniumpropyl)-4-(6-(4-(Diethylamino) Phenyl) Hexatrienyl) Pyridinium Dibromide) dye (Invitrogen™) to label endosomes for 8 minutes ...
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bioRxiv - Developmental Biology 2024Quote: HEK293T cells were transferred with C-terminal HA-tagged Kayak and N-terminal GFP-tagged Jun-epi or Kayak using Lipofectamine 2000 (11668030, Thermo Fisher). The GFP-trap agarose beads (ABIN509397 ...
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bioRxiv - Developmental Biology 2024Quote: ... five 3rd instar larval brains were dissected and homogenized in 30 μl lysis buffer (N-Per lysis buffer (Thermo Fisher, 87792) containing 0.1 mM DTT and 1:100 diluted proteinase inhibitor cocktail (Sigma ...
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bioRxiv - Developmental Biology 2024Quote: ... blots were incubated with primary antibodies (HA-Cell Signaling: #2367; Hsp90-Thermo Fisher: MA1-10372; N-cadherin-Thermo Fisher: 33-3900) overnight at 4°C ...
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bioRxiv - Immunology 2024Quote: Plasmodium falciparum 3D7 CSP was used as a reference to generate an N-terminal immunogen conjugated to KLH (Thermo Fisher Scientific). Murine studies were performed under approved protocol 22-0007 ...
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bioRxiv - Cancer Biology 2024Quote: ... Glutathione determinations were normalized to the protein content of the insoluble pellet from the SSA extracts 2.5% SDS in 0.1 N bicarbonate using the BCA Protein Assay Kit (Thermo Scientific, Rockford, IL).
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bioRxiv - Cancer Biology 2023Quote: ... Abcam, USA) (Anti-E-cad/N-cad/Vimentin; 1:200, Cell Signalling, USA) (Rhodamine phalloidin dye, 6.6 µM, ThermoFisher Scientific, USA) at room temperature for 1 hour ...
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bioRxiv - Cell Biology 2023Quote: ... MEFs were transfected with the isolated N-TAP progranulin plasmid (courtesy of the UF CTRND) using Lipofectamine LTX Reagent with PLUS Reagent (Invitrogen 15338030) according to its included protocol ...
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bioRxiv - Cancer Biology 2023Quote: Small RNA (<200 bp) was isolated from fibroids and matched myometrium (n= 5) using mirVana miRNA isolation kit (Thermo Fisher Scientific) according to manufacturer’s protocol ...
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bioRxiv - Physiology 2023Quote: ... Immunoreactivity was detected using the ECL Western blot detection reagent LumiGLO (KPL) and exposed to Super RX-N films (Fujifilm) or revealed with iBright™ Imaging System (ThermoFisher). Protein expression was normalized to α-actinin or Gadph ...
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bioRxiv - Developmental Biology 2023Quote: ... and connective tissue were scraped away from the biopsy before digesting O/N at 4°C in HBSS without Ca2+ and Mg2+ (Thermo Fisher) containing dispase at a final concentration of 500 caseinolytic units/mL (Corning ...
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bioRxiv - Molecular Biology 2023Quote: ... N-terminally truncated PPR56 coding sequences were amplified with classic PCR approach using Phusion High-Fidelity DNA Polymerase (Thermo Fisher Scientific) as described [23] to retain 14 native amino acids upstream of the most N-terminal completely retained PPR (Fig ...
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bioRxiv - Plant Biology 2023Quote: ... and AtILR1 open reading frame sequences deleted of the 25-N-terminal signal peptide-encoding codons were amplified using Phusion Taq Polymerase (Thermo Fisher) prior to cloning into pETM11 plasmid in the BL21 (DE3 ...
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bioRxiv - Neuroscience 2023Quote: ... centrifuged at 300g for 5 minutes at 4°C and homogenized in cold N-PER Neuronal Protein Extraction Reagent (Thermo Fisher) or cold RIPA lysis buffer (Thermo Fisher ...
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bioRxiv - Cell Biology 2023Quote: ... All the IHO1 truncations were expressed with the 3C HRV cleavable N-terminal MBP tag in suspension culture of High Five™ Cells (Invitrogen) in Sf-900™ III SFM medium ...
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bioRxiv - Bioengineering 2023Quote: ... The levels of RNA corresponding to the N protein-encoding gene of SARS-CoV-2 were measured using the TaqMan Fast Virus 1-step Master Mix (Thermo Scientific). Each 20-μL reaction mixture contained 5.0 μL of 4× TaqMan Fast Virus 1-Step Master Mix ...
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bioRxiv - Developmental Biology 2023Quote: Stem cells were grown on culture plates coated with 5micro μg ml-1 recombinant human Vitronectin (rhVTN-N, Life Technologies, #A14700). HESCs were grown in mTeSR1 (StemCell Technologies ...
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bioRxiv - Microbiology 2023Quote: ... prior to a one-hour incubation with SARS-CoV-2 N protein antibody (clone 1C7C7) at a 1:300 dilution and a AF594-conjugated Goat-anti-mouse secondary (ThermoFisher, A11005). Slides were counterstained with DAPI and scanned on an Akoya Polaris Vectra imaging system ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 2-(N-[7-nitrobenz-2-oxa-1, 3-diazol-4-yl] amino)-2-deoxyglucose (2-NBDG) (Molecular Probes-Invitrogen, CA, USA) was used to assess glucose uptake in Caco2 cells ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 2-(N-[7-nitrobenz-2-oxa-1, 3-diazol-4-yl] amino)-2-deoxyglucose (2-NBDG) (Molecular Probes-Invitrogen, CA, USA) was used to assess glucose uptake in Caco2 cells ...
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bioRxiv - Cell Biology 2023Quote: ... The PSA-NCAM+ cells were re-plated at a density of 50 × 103 cells per cm2 to the plate coated with vitronectin (VTN-N, 5 μg/mL; ThermoFisher Scientific) and laminin (rhLaminin-521 ...
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bioRxiv - Neuroscience 2023Quote: The forebrain sections were incubated with Blue NeuroTrace Fluorescent Nissl Stains (1:100 dilution by PBS, N-21479, Thermo Fisher Scientific) at room temperature for 2-3 hours ...
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bioRxiv - Plant Biology 2023Quote: ... OLV-FL or one of the truncated OLV versions were shuttled simultaneously with PYE into the destination vector pBiFC-2in1-NN (Grefen and Blatt, 2012) (N-terminal nYFP and cYFP fusions) via multisite LR reaction (Gateway, Thermo Fisher). Hereby pBiFC-2in1-NN:OLV-FL-PYE and pBiFC-2in1-NN:PYE-OLV-FL (additionally all truncated OLV versions were cloned into pBiFC-2in1-NN combined with PYE as described for OLV-FL ...
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bioRxiv - Plant Biology 2023Quote: ... For N-terminal fusions the pDONR entry clones with PYE and OLV sequences were used for Gateway LR reaction (Gateway, Thermofisher, Scientific) to generate the destination vectors based on pH7WGY2 (N-terminal YFP ...
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bioRxiv - Biochemistry 2023Quote: ... Dried lysed cellular lysate was digested using a solution of 5 nanogram/microliter LCMS grade trypsin (Pierce) in 0.1% n-Dodecyl-beta-Maltoside Detergent (DDM, Thermo Fisher, 89902) and 50mM TEAB ...
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bioRxiv - Physiology 2023Quote: ... the tissue was stained with NeuroTrace™ green fluorescent Nissl stain (1:200 in PBS, Life Technologies, catalog No. N-21480) for 20 min at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... then reverse transcribed using random primers with a N-terminal amine group and Revert-aid H-minus reverse transcriptase (Thermo Fisher). On the second day ...
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bioRxiv - Cell Biology 2024Quote: ... Glucose was traced using 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG, Invitrogen, ThermoFisher, cat. #N13195) combined with 0,57 mg/mL 40kDa tetramethyl-rhodamine Dextran (Invitrogen ...
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bioRxiv - Biochemistry 2023Quote: ... Dried cell lysate was digested using a solution of 5 nanogram/microliter LCMS grade trypsin (Pierce) in 0.1% n-Dodecyl-beta-Maltoside Detergent (DDM, Thermo Fisher, 89902) and 50mM TEAB ...
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bioRxiv - Biophysics 2023Quote: Plasmids encoding and expressing M with an N-terminal 6xHis tag and with and without SUMO tag fusions were designed in VectorNTI software (ThermoFisher Scientific) and synthesized de novo by GENEWIZ/Azenta Gene Synthesis service ...
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bioRxiv - Biochemistry 2023Quote: ... coli Methionine Sulfoxide Reductase A and B containing N-terminal His tags downstream of a T7 promoter were synthesized (Invitrogen GeneArt) and transformed into BL21(DE3 ...
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bioRxiv - Immunology 2023Quote: ... as well as between stratified patient plasma samples (n=7) over three time points using an IL-23 Human ELISA Kit (BMS2023-3, ThermoFisher, Canada), as per manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: From Expi293 cells: Constructs in pCMV10 vector with N terminal 3xFLAG tag were transfected into Expi293F cells (A14527; Thermo Fisher Scientific) with PEI (Polyscience ...
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bioRxiv - Immunology 2023Quote: COS-7 cells were cultured for 12 h on glass coverslips in a 24-well plate and then co-transfected with pcDNA3.1-NOX2-N-Clover and EROS-C-mRuby2 using Lipofectamine 3000 (Thermo Fisher Scientific) for 24 h at 37°C ...
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bioRxiv - Bioengineering 2023Quote: ... and 474/238), or fetal bovine serum (FBS, n = 542/415, 384/184, and 459/308) using a live/dead kit (Thermofisher, #L3224). Cell suspensions were centrifuged ...
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bioRxiv - Neuroscience 2023Quote: ... 1X N-2 (Thermo Fisher Scientific, 17502-048], 1X B-27 [Thermo Fisher Scientific, 17504-044], 1mM L-Glutamine [Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... stained with Ghost-510 and 2-(N-Nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose (2-NBDG; 60 µM) (Thermo Fisher Scientific) (30 min at 37 C) ...
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bioRxiv - Microbiology 2023Quote: ... the membrane was incubated O/N at 4°C with gentle agitation with 1:2000 goat anti-human complement C3 antibody (Thermo Scientific) diluted in 1X TBS/0,5% Tween/3% BSA ...
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bioRxiv - Microbiology 2023Quote: ... N (75 fmol) and L (25 fmol) proteins and the T7 RNA polymerase (50 fmol) using lipofectamine 3000 (Invitrogen™ L3000150) for 3 h at 37°C ...
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bioRxiv - Microbiology 2024Quote: ... M and Enn protein constructs composed of their N-terminal 100 amino acids were purified only on Ni2+-NTA resin (Thermofisher Scientific) with the size exclusion chromatography step omitted ...
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bioRxiv - Evolutionary Biology 2024Quote: ... purified proteins were chemically labelled at the N-terminus with TFP-AlexaFluor-488 and/or AlexaFluor-568 NHS ester (ThermoFisher Scientific) following the procedure provided by the manufacturer.
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bioRxiv - Developmental Biology 2024Quote: ... the exoA coding sequence was fused with a C-terminal mCherry tag followed by the N-terminal half of GFP sequence and inserted into the pIB-V5 vector (Thermo Scientific). Plasmid SOD2-cGFP was previously published 43 ...
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bioRxiv - Developmental Biology 2024Quote: Retroviral particles for the generation of DN-KASH and Delta-PP lines were generated in HEK293T cells expressing retroviral packaging plasmids (gift from N. Montserrat) and transfected using Lipofectamine™ 3000 Transfection Reagent (Invitrogen). The viral titer collected 2-days post transfection was used to infect Rex1-GFP mESCs overnight ...
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bioRxiv - Biochemistry 2024Quote: INIP and the TRR complex were labeled on N-termini using AF488 dye (Alexa Fluor 488 carboxylic acid succinimidyl ester, Thermo Fisher). Before labeling ...