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Citations for Thermo Fisher :
1101 - 1150 of 1859 citations for N Acetyl Tizanidine d4 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... hiPS cells were maintained in 6-well plates coated with recombinant human vitronectin (VTN-N, Life Technologies, A14700) in Essential 8 medium (Life technologies ...
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bioRxiv - Neuroscience 2022Quote: ... mouse brains and CV-1 fibroblasts were lysed using N-PER Neuronal Protein Extraction Reagent (ThermoFisher catalog # 87792). Samples were resolved by SDSPAGE using 10% acrylamide/bis-acrylamide gels and transferred to 0.2 μm pore size nitrocellulose (Bio-Rad) ...
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bioRxiv - Molecular Biology 2022Quote: ... The tissues were individually homogenized with N-PER™ Neuronal Protein Extraction Reagent (Cat. #: 87792, Thermo Fisher Scientific) containing Halt Protease and Phosphatase Inhibitor Cocktail (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2022Quote: ... Coverslips were subsequently mounted on glass microscopy slides using Fluoromount G (Thermo Fisher, cat n°00-4958-02).
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bioRxiv - Neuroscience 2022Quote: ... Sections were subsequently mounted on glass microscopy slides with Fluoromount G (Thermo Fisher, cat n°00-4958-02).
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bioRxiv - Cell Biology 2022Quote: ... bone slices with a thickness of 0.2mm (BoneSlices.com) were labelled with N-hydroxysuiccinimide ester-activated Rhodamine fluorescent dye (ThermoFisher). Mature osteoclasts were seeded at a density of 100,000 cells/bone slice in a 96-well plate ...
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bioRxiv - Bioengineering 2022Quote: ... in IMDM/F12 medium supplemented with 1% (v/v) N-2 (Thermo Fisher Scientific, catalogue no. 17502-048), 1% (v/v ...
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bioRxiv - Immunology 2024Quote: ... The RIPR N-half was produced in HEK293F cells using the Expi293™ Expression System (Thermo Fisher Scientific) in the presence of 5 μM kifunensine (Abcam) ...
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bioRxiv - Immunology 2024Quote: ... or 48 hours post stimulation in the presence of 2.5μM IMD0354 (n=3) were collected into TRIzol reagent (15596026, ThermoFisher). RNA isolations were performed according to the manufacturers protocol ...
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bioRxiv - Biochemistry 2024Quote: ... Individual samples were then labeled with isobaric tags using commercially available TMTsixplex (Thermo Fisher Scientific, P/N 90061) kits ...
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bioRxiv - Bioengineering 2024Quote: ... 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG, Invitrogen, Cat # N13195) and incubated for 30 minutes in their respective culture conditions ...
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bioRxiv - Neuroscience 2023Quote: ... hiPSCs were maintained in six-well plates coated with recombinant human vitronectin (VTN-N, Thermo Fisher Scientific, A14700) in Essential 8 medium (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... In vitro transcribed RNA of SARS-CoV-2 N-gene was synthesized with MEGAscript T7 (Thermo Fisher Scientific) using PCR product generated from SARS-CoV-2 WA1 cDNA and primers N0.5_F and N3-5_R (Eurofins ...
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bioRxiv - Cell Biology 2023Quote: ... X-biotin-PE [N-((6-(biotinoyl)amino)hexanoyl)-1,2-dihexadecanoylsn-glycero-3-phosphoethanolamine] was obtained from Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... Isopropyl-β-D-thiogalactopyranoside (IPTG) and 3-(N-morpholino) propane sulfonic acid (MOPS) were purchased from Fisher Scientific. MTSES was purchased from Biotium.
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bioRxiv - Microbiology 2022Quote: SK-N-SH cells were first transfected with 20 nM of siRNA against TSG101 using Lipofectamine RNAiMAX (Invitrogen). At 48 h post-transfection of siRNA ...
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bioRxiv - Neuroscience 2022Quote: Whole mouse brains were homogenized with a Dounce tissue grinder in neuronal protein extraction reagent (N-PER) (ThermoFisher) containing protease/phosphatase inhibitors (Cell Signaling #5872) ...
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bioRxiv - Microbiology 2022Quote: ... was synthesized as a custom peptide with N-terminal acetylation and C-terminal amidation (Life Technologies Europe Bv).
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bioRxiv - Molecular Biology 2023Quote: ... Human PKCβII was YFP-tagged at the N-terminus in a pcDNA3 vector using Gateway Cloning (Life Technologies) as described in (6) ...
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bioRxiv - Developmental Biology 2023Quote: ... BICs and differentiated AIC-N hESC clumps was isolated with the TRIzol™ Reagent (Thermo Fisher Scientific, 15596018). The 2×150 bp paired end libraries were sequenced with Illumina HiSeq X Ten instrument ...
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bioRxiv - Bioengineering 2023Quote: Cell culture medium Essential 8 (E8) Flex Medium and recombinant human vitronectin (rhVTN-N) were purchased from Thermofisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: Cloning of full-length N-terminal strepII-tagged hItln1 into the pcDNA4/myc-HisA vector backbone (Life Technologies) was described previously (14) ...
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bioRxiv - Cell Biology 2023Quote: ... HSPCs were incubated with 20µM NBD C6-Ceramide (6-((N-(7-Nitrobenz-2-Oxa-1,3-Diazol-4-yl)amino)hexanoyl)Sphingosine) (Invitrogen) for 30 minutes at 4°C for Golgi staining or Cytopainter (Abcam ...
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bioRxiv - Cell Biology 2023Quote: Primary human parenchymal lung fibroblasts from non-COPD and COPD patients (n=8) were grown in DMEM (Gibco) supplemented with 5% FBS and 2% antibiotics at 37°C and 5% CO2 ...
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bioRxiv - Plant Biology 2023Quote: ... with an N-terminal 6xHis tag followed by SUMO fusion protein using Gibson assembly protocol (Thermo Fisher Scientific). Protein was expressed in E ...
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bioRxiv - Neuroscience 2023Quote: ... cultured with neuronal maturation medium (BrainPhys Basal Medium [Stemcell Technologies], B-27 and N-2 supplements [Thermo Fisher Scientific] ...
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bioRxiv - Immunology 2023Quote: ... After the indicated time cells were lysed in 1% n-Dodecyl-β-D-Maltoside (DDM, Thermo Scientific #89903) in lysis buffer (30 mM Tris pH 7.4 ...
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bioRxiv - Molecular Biology 2024Quote: ... Prior to synthesizing codon-optimized gene fragments for the initial C- and N-terminus cassettes (GeneArt Strings, ThermoFisher), uniform cloning sequences were introduced around the cassette and lox sites to flank the selectable marker region ...
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bioRxiv - Neuroscience 2024Quote: ... or RGS14 (n=3, 1:500, mouse anti-RGS14 #75-170, Neuromab)/goat anti-mouse IgG2a (Life Technologies).
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bioRxiv - Cell Biology 2024Quote: ... HT-1080 cells were lysed by NP-40 buffer with 25 mM N-ethylmaleimide (NEM, 23030, Thermo Fisher) and protease inhibitor (04693116001 ...
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bioRxiv - Developmental Biology 2024Quote: ... Sequentially labeled cells for N-cadherin MO- or MM-containing explant experiments were grown in Neurobasal media (ThermoFisher) supplemented with B27 (1% ...
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bioRxiv - Immunology 2024Quote: ... Cells were then incubated for 30min at 37°C with 150µM of 2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl) amino]-2-deoxy-D-glucose (ThermoFisher).
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bioRxiv - Cancer Biology 2021Quote: ... was validated by STR analysis using AmpFLSTR™ Identifiler™ Plus PCR Amplification Kit (Cat n:4486467, ThermoFisher Scientific) using the provided protocol ...
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bioRxiv - Neuroscience 2021Quote: ... The cDNA encoding the short transcriptional variant of human DA D2 receptor with an N-terminal FLAG tag (SF-D2R-S) was inserted into mammalian expression vector pcDNA 3.1(+) (Invitrogen)62 ...
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bioRxiv - Cell Biology 2020Quote: ... ovaries from randomly cycling female mice (FVB/N, 6 weeks old) were collected and incubated in 0.25% Trypsin/PBS (Invitrogen) (37 °C ...
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bioRxiv - Immunology 2021Quote: ... cells were incubated in 2-(N-(7-nitrobenz-2-oxa-1,3-diaxol-4-yl) amino)-2-deoxyglucose (2-NBDG) (Invitrogen) (20 µM ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... over the course of 28 days and healthy controls (n = 37) and hybridized onto an HU133 Plus 2.0 GeneChip (Affymetrix) according to the manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2021Quote: ... protein was extracted from 3-week mature neurons using neuronal protein extraction reagent (N-PER) (Fisher Scientific, Waltham, MA) and protease inhibitor cocktail (Roche) ...
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bioRxiv - Molecular Biology 2020Quote: ... GFP-N was immunoprecipitated from two independent HEK293 cell pellets using 5μg of anti-GFP antibody (Life Technologies G10362). A total of 2% input material was obtained for size-matched control libraries (SMI ...
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bioRxiv - Neuroscience 2019Quote: ... LUHMES neuroblastoma cells (ATCC Cat# CRL-2927) were maintained DMEM/F12 (ATCC) supplemented with N-2 supplement (1%; Invitrogen) and human recombinant basic fibroblast growth factor (40 ng/ml ...
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bioRxiv - Neuroscience 2019Quote: ... Cortical hemispheres were dissected and homogenized in ice-cold homogenization buffer (N-PER Neuronal Protein Extraction Reagent (ThermoFisher Scientific) with cOmplete Protease Inhibitor Cocktail (Roche) ...
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bioRxiv - Developmental Biology 2019Quote: ... GenBank accession number BC057894.1) with an N-terminal hemagglutinin (HA) tag were reverse transcribed with SuperScript III Reverse Transcriptase (Invitrogen) and PCR-amplified using Phusion high-fidelity DNA polymerase (New England Biolabs ...
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bioRxiv - Bioengineering 2021Quote: Each sample (n=5) was placed in a 2 mL centrifuge tube containing 320 μL of collagenase II (Gibco, Thermo Fisher Scientific Inc. ...
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bioRxiv - Biochemistry 2021Quote: N-terminal GST-tagged MBNL1 (amino acids 1-260)59 was recombinantly expressed using BL21 Star (DE3) cells (Invitrogen) and protein expression induced using 0.5 mM IPTG at an OD600 = 0.6-0.7 for 2 hours at 37°C ...
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bioRxiv - Biochemistry 2020Quote: ... NosRcaΔC and NosRca-IFmut was labelled at the N terminus with the fluorophore Alexa Fluor 405 NHS ester (ThermoFisher) (∼2.2 ...
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bioRxiv - Neuroscience 2021Quote: DRGN and retinal cells were washed with PBS and total RNA (n = 6-9 wells/treatment) was extracted using Trizol reagent according to the manufacturer’s instructions (Invitrogen). Appropriate primers were used to validate each gene of interest using the specific primers (Table 1 ...
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bioRxiv - Neuroscience 2020Quote: ... Alexa Fluor® 555 goat anti rabbit IgG (Cat. N° A21429) was obtained from Life Technologies (Carlsbad, CA, USA), Alexa Fluor® 488 Goat Anti-Mouse IgG (Cat ...
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bioRxiv - Physiology 2021Quote: ... 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG, Thermo Fisher Scientific, USA) was dissolved in saline solution at 5 mg/ml ...
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bioRxiv - Neuroscience 2020Quote: Mouse midbrains (n = 5-6 per group per age) were collected and RNA was isolated using TRIzol Reagent (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... over the course of 28 days and healthy controls (n = 37) and hybridized onto an HU133 Plus 2.0 GeneChip (Affymetrix) according to the manufacturer’s recommendations ...