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Citations for Thermo Fisher :
1051 - 1100 of 1859 citations for N Acetyl Tizanidine d4 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... N/Tert-1 cells were cultured in keratinocyte serum-free medium (K-SFM) (Invitrogen; catalog no. 37010022) supplemented with bovine pituitary extract ...
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bioRxiv - Cell Biology 2023Quote: A cDNA encoding p38α was subcloned into pcDNA6 with an N-terminal myc tag (Invitrogen, Carlsbad, CA), and a kinase-dead (D168A ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples (n = 3 technical replicates, each condition) were sorted with an Attune NxT flow cytometer (Thermo Fisher) and the DNA content was analyzed for the percent of cells in G1 ...
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bioRxiv - Neuroscience 2023Quote: ... was added to 10 ml of ice-cold N-PER Neuronal Protein Extraction Reagent (# 87792, Thermo Fisher) by vigorous vortexing until the tablet was completely dissolved ...
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bioRxiv - Neuroscience 2023Quote: The resulting peptide mixture was resolved by liquid chromatography (LC) using an EASY n-LC (Thermo Scientific) UHPLC system with buffer A (2% v/v acetonitrile/0.1% v/v formic acid ...
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bioRxiv - Molecular Biology 2023Quote: ... Then recombination step was done (Gateway® LR Clonase® II Enzyme mix, INVITROGEN, cat n°11791020) in the final mammalian cellular expression plasmid pEF-Dest51 (InvitrogenTM) ...
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bioRxiv - Developmental Biology 2023Quote: ... and carried out on an Applied Biosystems Vii 7 RT-PCR system (Thermo Scientific P/N 4453552). Validated gene-specific primers in this study can be found in Table S14 ...
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bioRxiv - Neuroscience 2023Quote: ... the pellet (synaptosomal fraction) was re-suspended in N-PER Neuronal Protein Extraction Reagent (ThermoFisher Scientific; 87792). The homogenate was transferred to an appropriate microcentrifuge tube and incubated on ice for 10 minutes ...
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bioRxiv - Microbiology 2023Quote: ... Vero E6-TMPRSS2 cells were fixed with 4% paraformaldehyde and stained with anti-N (Invitrogen; MA1-7403). The number of fluorescent foci was measured using a Cytation 5 Cell Imaging Multimode Reader (BioTeK).
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bioRxiv - Cancer Biology 2023Quote: N/Tert-1 cells were cultured in keratinocyte serum-free medium (K-SFM) (Invitrogen; catalog no. 37010022) supplemented with bovine pituitary extract ...
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bioRxiv - Bioengineering 2023Quote: Bone explants (n=8) were cultured in medium consisting of α-MEM (41061, GibcoTM, Thermo Fisher Scientific), 10% v/v FBS (HycloneTM ...
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bioRxiv - Biochemistry 2023Quote: ... Digested peptides were eluted through centrifugation and labeled using commercially available TMTsixplex tags (ThermoFisher, P/N 90061). After labeling ...
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bioRxiv - Cancer Biology 2024Quote: METTL3 siRNAs were used to induce transient knockdown in SK-N-BE(2) cells with RNAiMAX (ThermoFisher) reagent ...
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bioRxiv - Cell Biology 2020Quote: ... pH 7.5 in 1X PBS then treated with 400 μg/ml sulfo-N-hydroxysulfosuccinimide-biotin (Thermo Fisher Scientific) prepared in the washing buffer for 40 min on an orbital shaker at 4°C ...
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bioRxiv - Genetics 2021Quote: ... and Tvrm323 mice (n = 5) at one month of age by homogenizing posterior eyecups in TRIzol (Thermo Fisher) using a gentleMACS dissociator (Milteny Biotec) ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells that became adherent during the differentiation process were detached using cell dissociation buffer (Invitrogen, cat n°13151014) and pooled with non-adherent cells ...
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bioRxiv - Synthetic Biology 2019Quote: ... 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl) was purchased from Invitrogen. Calcein dye ...
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bioRxiv - Immunology 2019Quote: Bacterial cultures were incubated O/N in a 5 μM solution of Draq5 Fluorescent Probe Solution (ThermoFisher Scientific). Bacterial suspensions were washed 3 times with PBS before RAW264.7 macrophages were infected.
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bioRxiv - Biochemistry 2020Quote: ... were coated with 5 μg/mL anti-DPEEAE neoepitope antibody (Cat n. PA1-1748A, Life Technologies, Paisley, UK) in carbonate buffer pH 9.6 (16 h ...
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bioRxiv - Microbiology 2021Quote: ... with SARS-CoV-2 N-specific primers (EV Table 1) on a QuantStudio 6 Flex thermocycler (Applied Biosystems). Standard curve was performed in parallel using purified SARS-CoV-2 viral RNA ...
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bioRxiv - Neuroscience 2020Quote: Total RNA was isolated from fly heads (n=15 per condition) using TRIzol reagent (Thermo Fisher Scientific, 15596026) and quantified on NanoDrop 2000c (Thermo Scientific) ...
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bioRxiv - Bioengineering 2021Quote: 500 μg of RNA was extracted from hBMMSC-seeded ADC gels (n = 3) using TRIzol reagent (Invitrogen, USA) as per manufacturer’s instructions following the initial 48 h of mechanical stimulation ...
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bioRxiv - Bioengineering 2021Quote: ... 10 μg of extracted proteins (n = 3) were solubilized in 1 × NuPage® LDS Sample buffer (Invitrogen, USA) and was run in a 15 well 12% Tris-Gly precast gel (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... The first strand was reverse transcribed with primer FR26RV-N using Superscript III enzyme (Life Technologies, Carlsbad, CA), followed by complementary strand synthesis using Sequenase polymerase (Affymetrix ...
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bioRxiv - Biochemistry 2022Quote: ... Individual samples were then labeled with isobaric tags using commercially available TMTsixplex (Thermo Fisher Scientific, P/N 90061) kits ...
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bioRxiv - Bioengineering 2022Quote: ... carrying an additional GS linker in the N-terminus were chemically synthesised (Invitrogen, by Thermo Fisher Scientific UK) and annealed ...
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bioRxiv - Bioengineering 2022Quote: ... carrying an additional GS linker in the N-terminus were chemically synthesised (Invitrogen, by Thermo Fisher Scientific UK) and annealed ...
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bioRxiv - Microbiology 2021Quote: Cell free glycosylation was conducted in S30 buffer with 0.1% n-dodecyl-β-d-maltopyranoside (DDM; Thermo Scientific) and 10 mM MnCl2 (Across Organics) ...
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bioRxiv - Biochemistry 2019Quote: ... 5 mM MgCl2) with 1.5% n-Dodecyl β-D-maltoside (DDM) and 1X Halt Protease Inhibitor (Thermo Scientific) for 30 minutes on ice ...
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bioRxiv - Plant Biology 2020Quote: Venus was fused to either the C or the N terminus of Pit using the Gateway system (Invitrogen). The Pit-Venus ...
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bioRxiv - Genetics 2020Quote: mRNA was collected from groups of 10 whole larvae (n=3–4 replicates per line) using Trizol (ThermoFisher) and reverse transcribed to cDNA using SuperScript III Reverse Transcriptase (ThermoFisher) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Samples of the visceral yolk sac (n=6/group) were immersed in RNA Later solution (Invitrogen, MA, USA) and frozen at −20°C until further processing ...
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bioRxiv - Immunology 2020Quote: ... activated for 30 min on a rotor at RT by addition of Sulfo-N-Hydroxysulfosuccinimide (Thermo Fisher Scientific) and 1-Ethyl-3-(3-dimethylaminopropyl ...
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bioRxiv - Molecular Biology 2020Quote: RT-RCA was performed in 20 µL with Maxima H Minus Reverse Transcriptase (Thermo Fisher, cat n°EP0752) under the following conditions ...
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bioRxiv - Molecular Biology 2020Quote: ... RPFs were generated by treating the diluted lysate with 12.7 U of RNase I (Ambion, cat. n°AM2295) at room temperature for 45 min (as described in Clamer et al. ...
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bioRxiv - Microbiology 2020Quote: ... The extraction solvent consisted of 30 g/L of tri-n-octylphosphine oxide (TOPO, Acros Organics, Geel, Belgium) in mineral oil (Sigma Aldrich ...
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bioRxiv - Plant Biology 2020Quote: ... the CDS of RFP with a N-terminal AvrII restriction site was amplified and cloned into pDONR221 (Invitrogen) generating a pEntry clone ...
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bioRxiv - Synthetic Biology 2019Quote: ... the MS was calibrated using ESI Negative on Calibration Solution (P/N 88324, Thermo Scientific, San Jose, USA).
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bioRxiv - Biochemistry 2021Quote: ... The N-terminal 8xHis-TwinStrep-GFP-tagged NKCC1 constructs were expressed in GripTite HEK293 MSR cells (Thermo Fisher) in 24 well plates ...
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bioRxiv - Microbiology 2021Quote: ... the drugs were diluted from 0.78-25µM in 200µL of MOPS [3-(N-morpholino) propanesulfonic acid] buffered RPMI 1640 (Life Technologies), pH 7 ...
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bioRxiv - Cell Biology 2020Quote: ... Inserts were then subcloned into pCS2+ N’ HA tagged vectors that had been converted into Gateway® (Invitrogen) destination vectors ...
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bioRxiv - Cell Biology 2019Quote: ... was neutralized with reconstitution buffer (2.2% NaHCO3, 0.05 N NaOH, and 200 mM HEPES) and diluted with 5× DMEM (Gibco). MDCK cells (1.5 × 105 cells/ml of collagen gel ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... in six-well feeder-free culture dishes coated with 5 μg/mL vitronectin (VTN-N; Thermo Fisher Scientific). When seeding the cells ...
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bioRxiv - Immunology 2021Quote: ... or for intracellular expression using the pYES2/NTC vector (N-terminal Xpress and C-terminal V5 epitope, Invitrogen). The pYD1 system used the yeast internal Apa1/Ap2 system ...
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bioRxiv - Immunology 2021Quote: ... Tryptic peptides (2μg) were loaded onto a C18 trap (75μm x 2cm; Acclaim PepMap 100, P/N 164946; ThermoFisher) at a flow rate of 2μL/min of solvent A (0.1% formic acid in LC-MS grade H2O) ...
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bioRxiv - Molecular Biology 2021Quote: ... N-terminal amino acid sequencing was performed using the Procise 494HT Edman sequencer (Applied Biosystems, Foster City, CA) with 610A data analysis module ...
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bioRxiv - Neuroscience 2020Quote: ... N-glycopeptides from two enrichment methods were combined and desalted by C18 tips (Thermo Fisher Scientific, Cat #87784) following the manufacturer’s instructions and resuspended in 30 μL 100 mM TEAB buffer ...
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bioRxiv - Biochemistry 2020Quote: ... 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) and N-hydroxysulfosuccinimide (Sulfo-NHS) were obtained from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Genetics 2020Quote: ... Purified N-HIS-FAM19A5 was then digested overnight at 30°C with AcTEV protease (Thermo Fisher Scientific, USA) to remove the His-tag ...
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bioRxiv - Cancer Biology 2022Quote: ... U251(N) and primary GBM cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) high glucose (GibcoTM, ThermoFisher) with 10 % FBS (P30-3031 ...