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Citations for Thermo Fisher :
9401 - 9450 of 10000+ citations for 5 Nitrofluorescein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... The cells were cultured in a humidified incubator at 37C in 5% CO2 and 95% O2 using a high glucose DMEM (ThermoFisher) containing 10% fetal bovine serum (Life Technology ...
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bioRxiv - Cell Biology 2022Quote: ... Each sample was centrifuged at 200 × g for 5 min and pellets were incubated with 40 nM DiOC6 (D273, Invitrogen) for 15 min at 37°C and 5% CO2 to assess mitochondrial membrane potential ...
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bioRxiv - Biophysics 2022Quote: ... Matthias Mack (Signoret et al., 2000)MC-5 was fluorescently labeled using DyLight 650 NHS ester coupling kit (Thermo Fisher) with a dye:protein coupling ratio of 1.57:1.
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bioRxiv - Neuroscience 2022Quote: ... at a thickness of 300μM as before93,94 and placed in prewarmed aCSF with 5% CO2 and 95% O2 for 30m before transferring to Neurobasal Medium (ThermoFisher). They were treated with vehicle or 500μM glutamate for 3h at 37°C ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were grown at 5% CO2 and 37 °C in DMEM high glucose media supplemented with 10% heat inactivated fetal bovine serum (FBS) (Gibco), penicillin/streptomycin (Gibco) ...
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bioRxiv - Bioengineering 2022Quote: ... cells were washed 3 x 5 min with PBS before adding secondary antibody (1:200 Alexafluor-488 goat anti-mouse, Invitrogen) and rhodamine phalloidin (1:100 ...
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bioRxiv - Molecular Biology 2022Quote: ... NC) or plasmid with HAX1 gene with tag on 3’end or 5’end of the gene (LipofectamineTM2000, ThermoFisher Scientific). Cells were detached and seeded on 100mm plates to grow single colonies (selection ...
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bioRxiv - Immunology 2022Quote: Two-millimeter tissue sections were excised 5 cm from the distal end of the small intestine (ileum) and stored in RNAlater (ThermoFisher) overnight at 4°C ...
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bioRxiv - Physiology 2022Quote: ... 50 μg of proteins of tissue lysates were boiled for 5 min at 95°C in NuPAGE LDS sample buffer containing 1x reducing agent (Invitrogen). Lysates were separated by SDS–PAGE using the XCell SureLock Electrophoresis Cell on NuPAGE Novex 4%–12% Bis-Tris Protein Gels (Invitrogen) ...
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bioRxiv - Bioengineering 2022Quote: ... DAPI was used as a counterstain at 300nM in 1xPBS for 5 minutes at room temperature and then mounted in mounting medium ProLong Diamond Antifade (ThermoFisher). Imaging was done at room temperature on a Nikon Eclipse Ti microscope with an UltraView VoX imaging system (PerkinElmer ...
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bioRxiv - Biochemistry 2022Quote: ... 5 μm particle size) and an analytical column (PepMap C18, 500 x 0.075 mm, 2 μm particle size (Thermo Scientific), coupled to a Q Exactive HF-X Orbitrap (with HCD ...
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bioRxiv - Biochemistry 2022Quote: ... HDLM-2 and K562 cells were obtained from the AstraZeneca cell bank (Alderley Park, UK) and maintained at 37°C in 5% CO2 and grown in DMEM or RPMI-40 (Gibco) with 10% fetal bovine serum (ThermoFisher) ...
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bioRxiv - Cell Biology 2022Quote: ... stably expressing GFP-Tubulin obtained from Franck Perez lab were grown at 37°C and 5% CO2 in DMEM/F12 (31331028, Gibco) supplemented with 10% fetal bovine serum (10270106 ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant was decanted and incubated with a 5 mL 50% slurry of NiNTA (Thermofisher Scientific, Saint-Laurant, QC, Canada) resin for one hour with constant inversion ...
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bioRxiv - Bioengineering 2022Quote: ... 721.221 cell lines were kept in a humidified incubator at 37°C with 5% CO2 and cultured in complete RPMI1640 medium supplemented with 2 mM GlutaMAX (Invitrogen), 10% FBS (Sigma),10 mM HEPES pH 8.0 (Thermo Fisher) ...
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bioRxiv - Cancer Biology 2022Quote: ... All cell line were maintained at 37°C and 5% CO2 in the presence of 100 unit/ml penicillin and 100 μg/ml streptomycin (Gibco) and were routinely tested for mycoplasma using MycoAlert mycoplasma detection kit (Lonza) ...
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bioRxiv - Biochemistry 2022Quote: ... the gel was rinsed in Milli-Q water for 5 minutes and stained for 20 minutes with 1x SYBR gold nucleic acid stain (Invitrogen) in 1x TBE ...
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bioRxiv - Neuroscience 2022Quote: ... were cultured at 37°C and 5% CO2 in high glucose Dulbecco’s Modified Eagle Medium with GlutaMAX™ Supplement (DMEM; Gibco) supplemented with 1 mM sodium pyruvate (Gibco) ...
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bioRxiv - Immunology 2022Quote: ... were pulsed for 1 hr with 10μg/ml influenza virus NP(366-374) peptide and were subsequently labeled for 10 min at 37°C with 5 μM CFSE (Invitrogen) (CFSEhigh ...
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bioRxiv - Cancer Biology 2022Quote: ... All cells were maintained at 37°C and 5% CO2 in high humidity and cultured in high glucose DMEM (Gibco) supplemented with 10% (vol/vol ...
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bioRxiv - Molecular Biology 2022Quote: ... were used at a final concentration of 5 nM and mixed with 1.3 µl of Lipofectamine RNAiMAX (Thermo Fisher Scientific). The transfection mix was pre-spotted onto 24-well plates for 30 min at RT prior the addition of 7.0x104 A549 cells per well ...
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bioRxiv - Molecular Biology 2022Quote: ... Paraffin-embedded human tissues and hind paws were cut into 5 μm sections with the Microtome HM355S (Thermo Fisher Scientific) and transferred onto microscope slides.
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bioRxiv - Neuroscience 2022Quote: ... Coverslips were rinsed 3 times for 5 minute intervals with PBS and mounted on microscope slides (Fisher Scientific, 22-178277) using ProLong Glass Antifade Mountant (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... The protein complex was purified by a Superdex 200 16/60 column in a buffer containing 5 mM HEPES pH 7.5 (Life Technologies) and 150 mM NaCl (Quality Biological ...
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bioRxiv - Immunology 2022Quote: ... 0.1 mM EDTA) for 5 minutes at room temperature and then neutralized with B cell media (RPMI 1640 with L-glutamine (Gibco) supplemented with 15% fetal bovine serum (Corning) ...
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bioRxiv - Immunology 2022Quote: ROS production was assessed after 2.5 h of stimulation as previously described (59) with 5 μM of the CellROX™ green reagent (Thermofisher). Cells were acquired on an Attune NxT.
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bioRxiv - Neuroscience 2022Quote: Using a subcloned and sequenced PCR fragment (162 bp – Table S1) of the mouse 5-HT2AR cDNA (Zero Blunt TOPO PCR Cloning kit, Invitrogen), high specific-activity RNA probes were produced from a linearized plasmid (HindIII ...
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bioRxiv - Pathology 2022Quote: ... for 5 min at 95 °C before they were loaded in Bolt™ 4–12 % Bis-Tris gels (Thermo Fisher). After separation ...
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bioRxiv - Physiology 2022Quote: Mouse NIH3T3 cells were transfected with pExpress-LoxBsr-FLAG-2xNLS-EGFP or pExpress-LoxBsr-FLAG-mGCNA then later selected for resistance to Blasticidin (5 µg/ml, catalog no. A11139-03, Gibco). Resistant clones were then screened for the expression of FLAG-2xNLS-EGFP or FLAG-mGCNA by immunoblotting of FLAG (see later in “Western Blotting” part) ...
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bioRxiv - Biochemistry 2022Quote: ... A total of 405 WT peptides were identified (Table 4) on the Fusion Lumos OrbiTrap mass spectrometer (mass tolerance <5 ppm) using Proteome Discoverer 2.2.0 software (Thermo Fisher Scientific).
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bioRxiv - Biochemistry 2022Quote: ... to final concentration 0.5 mg/ml protein and 6x dye and heated from 10 to 70 °C with fluorescence reading every 0.1 °C (4 to 5 sec) in a QuantStudioTM 7 Flex Real-Time PCR System (Life Technologies). Protein thermal melting curve were generated using the Protein Thermal Shift TM software ...
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bioRxiv - Developmental Biology 2022Quote: ... 5’L1-3’L2 orientation were inserted via recombination into a pCS2+ Gateway-converted vector (Custom vector conversion kit; Invitrogen). Details of the Gateway plasmid are available upon request ...
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bioRxiv - Bioengineering 2022Quote: ... Samples were boiled at 95°C for 5 min and loaded on SDS-PAGE (NuPAGE 4-12% Bis-Tris, Invitrogen). Efficiency of ligand coupling to the Ad capsid (% total hexon protein coupled ...
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bioRxiv - Developmental Biology 2022Quote: ... F 5’-GAACTGTCCAGATGCCCTTCCAGTT-3’ and R 5’-GCATCTGGACAGTTCTGGGAAGCCCG-3’) was cloned by PCR and ligated into the pEF6/V5-His TOPO plasmid vector (Invitrogen). FBLN7-V5-His vector was transfected into CHO cells (FBLN7-CHO ...
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bioRxiv - Immunology 2022Quote: ... Supernatants were collected with remaining adherent cells collected by 5 min incubation with Cell Dissociation Buffer (Gibco, Thermo Fisher Scientific) at 37°C and 5% CO2 ...
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bioRxiv - Immunology 2022Quote: ... Supernatants were collected with remaining adherent cells collected by 5 min incubation with Cell Dissociation Buffer (Gibco, Thermo Fisher Scientific) at 37°C and 5% CO2 ...
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bioRxiv - Microbiology 2022Quote: ... hiPSC-CMs were quickly washed with DPBS and incubated with SARS-CoV-2 at 5 multiplicity of infection (MOI) diluted in DMEM only (Gibco) for 1 h at 37 C ...
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bioRxiv - Microbiology 2022Quote: ... An equivalent of 0.25 midgut and 5 salivary glands were resolved in a NuPAGE™ 10% Bis-Tris Protein Gel (Invitrogen) under reducing conditions and transferred to a PVDF membrane Invitrogen™ Power Blotter Select Transfer Stacks ...
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bioRxiv - Cell Biology 2022Quote: Human embryonic kidney 293T (HEK 293T) cells (ATCC CRL-3216) were cultured at 37 °C and 5% CO2 in DMEM (Gibco) supplemented with 4.5 g/L glucose ...
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bioRxiv - Cell Biology 2022Quote: K-562 Chronic Myelogenous Leukemia cells (ATCC, CCL-243) were cultured at 37 °C and 5% CO2 in IMDM (Cat# 12440053, Gibco) supplemented with 10 % fetal calf serum (FCS ...
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bioRxiv - Immunology 2022Quote: ... complementary DNA (cDNA) was generated from extracted mouse plasma RNA using primer YB383 5’-TTTTTTTTTTTTTTTTTTTTTTTTRAAGCAC-3’ and enzyme Superscript III (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... Those at the early headfold to early somite stages were selected and cultured by the roller culture method under 5% CO2 at 37°C in 50-ml tubes containing DMEM or FluoroBrite DMEM (A1896701, ThermoFisher) supplemented with 75% rat serum ...
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bioRxiv - Cell Biology 2022Quote: ... and cells were passaged every 4-5 days using 0.5 mM EDTA (pH 8.0) (Thermo Fisher Scientific, Waltham, MA, USA).
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bioRxiv - Cell Biology 2022Quote: ... The membranes were again washed 3× in PBS for 5 min each and mounted onto slides using ProLong Gold Antifade (Invitrogen). Images of stained mTECs were obtained using an SP8 (Leica Microsystems ...
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bioRxiv - Biochemistry 2022Quote: ... 550 pmol of protein was then added to a tube containing 1.1 nmol of 5’Biotin(GGTTAG)2 DNA (Thermo Fisher) in a volume of 100 µL dilution buffer (20 mM HEPES-KOH pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... CRL-3216) were maintained at 37°C under 5% CO2 in Dulbecco’s modified Eagle’s medium (Gibco; cat. no. 12800-017) supplemented with 10% FBS (Gibco ...
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bioRxiv - Cancer Biology 2022Quote: ... Coverslips were then washed 3x for 5 mins with 1xPBS before mounting onto glass slides using ProLong Diamond anti-fade mountant (Invitrogen) and left to dry for 24hrs before imaging ...
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bioRxiv - Biochemistry 2022Quote: ... Protein samples were heated at 95°C for 5 minutes before loading and separated onto SDS-PAGE cassette (Thermo Fisher Scientific Bolt™ 4-12% Bis-Tris Plus Gels ...
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bioRxiv - Cancer Biology 2022Quote: ... and 0.06% Bromophenol Blue) at 98°C for 5 minutes and separated on 12% sodium dodecyl sulfate (SDS)-polyacrylamide gels (Invitrogen). Proteins were transferred to nitrocellulose membranes (Millipore ...
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bioRxiv - Microbiology 2022Quote: ... The inoculum was removed and cells washed 2× with PBS and 500 μL culture media (high glucose DMEM + 5% FBS (Gibco) + 1% antibiotic-antimycotic ...