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Citations for Thermo Fisher :
9401 - 9450 of 10000+ citations for 5 NITROFURFURYL ALCOHOL since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2019Quote: ... 5% [v/v] glycerol) was concentrated to 9 mg/mL (measured by UV absorbance in a NanoDrop spectrophotometer (Thermo Scientific) using the calculated molecular weight and estimated extinction coefficient ...
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bioRxiv - Molecular Biology 2019Quote: ... and the liver samples were taken and immediately submerged in 5 mL of RNA later® solution (Ambion, Courtabeuf, France) to preserve the integrity of RNA ...
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bioRxiv - Systems Biology 2019Quote: ... Cells were cultured in a humidified cell incubator at 37 °C with 5% CO2 conditions and split with TrypLE Express (Gibco, Invitrogen) every 2-3 days.
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Therapy-induced lipid uptake and remodeling underpin ferroptosis hypersensitivity in prostate cancerbioRxiv - Cancer Biology 2020Quote: Lipid peroxidation was measured by live ratiometric fluorescence microscopy of ATT pre-treated cells after incubation in 65 µl/well of serum-free RPMI media (0.2% lipid-free BSA) supplemented with 5 µM with BODIPY 581/591 C11 (Thermo Fisher) for 60 min at 37°C ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were washed three times with PBS for 5 min and incubated during 1 hour at RT with AlexaFluor (Invitrogen) secondary antibodies ...
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bioRxiv - Biophysics 2019Quote: 20-nm fluorescent dark red beads samples (Fig. 2d, Supplementary Fig. 5) were prepared using a 5.10−7 dilution of the initial solution (F8783, Thermo Fisher). We performed the dilution in PBS + 5% glucose to match the index of the dSTORM imaging buffer ...
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bioRxiv - Immunology 2019Quote: ... at 3:1 beads:cell ratio in T cell media (AIMV supplemented with 5% FBS, 10mM HEPES, 2mM GlutaMAX, 100 U/mL penicillin, and 100ug/mL streptomycin (Gibco)) ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were cultured in RPMI 1640 medium containing 10% fetal bovine serum and 2 mM L-glutamine at 37 °C in 5% CO2 and passaged at 70–90% confluency with 0.05% TrypLE Express (all from ThermoFisher Scientific).
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bioRxiv - Cell Biology 2019Quote: ... Protein expression was performed using a 2800 ml baffled shaker flask (Fisherbrand, BBV2800) as follows: The 5 ml of starter culture was added to 500 ml of MagicMedia (K6815, Invitrogen), supplemented with 50 µg/ml kanamycin sulfate and incubated as above for 24 h at 30 °C (for FGF2 or TGFβ3 ...
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bioRxiv - Biochemistry 2019Quote: ... Coverslips were washed three times for 5 min with PBS and incubated with 2 μg/mL goat anti-mouse Alexa Fluor 555 secondary antibody (Invitrogen,), in the dark for 1 h at 37 °C ...
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bioRxiv - Molecular Biology 2019Quote: ... Labeled DNA (500 ng) was precipitated during 30 min incubation at −20°C after adding 5 μl of mouse Cot-1 DNA (Invitrogen), 0.5 volume of 7.5 M ammonium acetate and 2.5 volumes of cold 100% ethanol ...
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bioRxiv - Neuroscience 2019Quote: ... The cultures were recovered in serum-containing media (Neurobasal media supplemented with 10% horse serum, 5% fetal bovine serum, and 2 mM glutamine [Gibco]) for 4 h and maintained in serum-free media (Neurobasal media supplemented with B-27 (1:50 diluted) ...
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bioRxiv - Genetics 2020Quote: ... For morphological analyses 105 cells were resuspended in 200 ml PBS and spun (5 min, 400 rpm) in a Cytospin 4 (ThermoFisher), before staining with modified Wright’s Stain on a Hematek (Bayer Health Care) ...
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bioRxiv - Developmental Biology 2019Quote: ... and mir152 specific primers with NCode miRNA First-Strand cDNA Synthesis qPCR Universal Primer in a fast mode SybrGreen reaction at the QuantStudio 5 (Thermofisher). We used RNU6B and RNU44 as endogenous controls ...
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bioRxiv - Molecular Biology 2019Quote: Huh-7 (human hepatoma) cells were maintained at 37 °C and 5% CO2 in Dulbecco’s Modified Eagle Medium (DMEM, Life Technologies) supplemented with 10% (vol/vol ...
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bioRxiv - Immunology 2019Quote: ... the blood system of the whole body was labelled by injecting 100 µl of WGA-Alexa Fluor 555 (5 mg/ml, Invitrogen) with 10 µl Heparin (100 units/ml ...
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bioRxiv - Biochemistry 2019Quote: ... The supernatant was analyzed by reversed-phase HPLC using a Hypersil GOLD aQ column (5 μm particle size, 150 × 4.6 mm, flow rate 0.5 ml at 52°C, Thermo Fisher Scientific) with a mobile phase of H2O-0.05% trifluoroacetic acid and a 25% acetonitrile gradient over 130 min ...
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bioRxiv - Systems Biology 2019Quote: ... 25 mM Tris-HCl pH 7.4, 150 mM NaCl, 1 mM EDTA, 1% NP-40 and 5% glycerol; Thermo Fisher) containing 1x protease inhibitor cocktail III (Calbiochem) ...
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bioRxiv - Cancer Biology 2020Quote: ... maintained in Dulbecco’s modified Eagle’s Medium (DMEM) with 10% FBS at 37°C and 5% CO2) were transiently transfected using Lipofectamine 2000 (Life Technologies). Retroviral particles were obtained and concentrated by centrifugation of the conditioned media (6000 × g overnight at +4°C) ...
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bioRxiv - Clinical Trials 2019Quote: ... and MDCK-SIAT cells (WHO CC London) were maintained at 37°C with 5% CO2 in Dulbecco’s modified Eagle’s Medium (DMEM) (Gibco-Life technologies) supplemented with 10% foetal bovine serum ...
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bioRxiv - Neuroscience 2019Quote: ... A sequence including the zinc finger binding sequence (5’-GTCATCCTCATC-3’) (Gross et al., 2013; Perez et al., 2008) upstream of the hsyn1 promoter was synthesized (ThermoFisher) and inserted using the MluI and EcoRI restriction sites to generate pAAV-ZFBS-syn-PSD95.FingR-dNES-CaMPARI2_F391W_L398V-CCR5TC ...
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bioRxiv - Plant Biology 2019Quote: ... Every qRT-PCR reaction was carried out in a 10μL volume containing: 5 μL SsoAdvanced Universal SYBR Green Supermix (Applied Biosystems), 1 uL template ...
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bioRxiv - Zoology 2020Quote: ... This extended COI fragment was amplified using the dgLCO1490 (5’-GGT CAA CAA ATC ATA AAG AYA TYG G-3’) and COI-R1 (5’-TGT TGR GGG AAA AAR GTT AAA TT-3’) degenerate primers (synthesized by Invitrogen) from Meyer et al ...
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bioRxiv - Immunology 2020Quote: ... Samples were diluted 1:5 in TE buffer and 6% were mixed with TaqMan Universal PCR Master Mix (Applied Biosystems). The sample mix and TaqMan assays were loaded separately into the wells of 96.96 Gene expression Dynamic Arrays (Fluidigm ...
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bioRxiv - Immunology 2019Quote: ... Migrated LCs were harvested after 48 hours culture of epidermal fragments in full culture media (RPMI, Gibco, UK, 5%FBS, Invitrogen, UK ...
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bioRxiv - Plant Biology 2019Quote: ... The 5’ RACE adapter (Supplementary Table S2) was then ligated to the 5’ ends of the + or –TAP treated RNA with T4 RNA ligase (Ambion). For this ...
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bioRxiv - Molecular Biology 2020Quote: ... 5 µl of 10X TURBO™ DNase Buffer and 1 µl of TURBO™ DNase (Art. No. AM2238, Invitrogen™) were added to the RNA and incubated at 37 °C for 30 minutes with gentle shaking ...
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bioRxiv - Cell Biology 2019Quote: ... COS-7 and U2OS cells were grown at 37°C with 5% CO2 in complete DMEM (Thermo Fisher Scientific, USA) containing 10% FBS and 1% L-glutamine ...
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bioRxiv - Cancer Biology 2019Quote: ... ssDNA (M13 primer ssDNA-M13 primer 5’-TGT-AAA-ACG-ACG-GCC-AGT-3’, paraformaldehyde (PFA) were purchased from ThermoFisher. PicoGreen variants were synthesized in house at ThermoFisher ...
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bioRxiv - Microbiology 2019Quote: ... burnetii total RNA extracted from LCVs (3dpi) infecting Vero (carA and metK) or THP-1 (cvpD) cells using a 5’ RACE System kit (Invitrogen) according to manufacturer protocols and with gene-specific primers (see S9 Fig) ...
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bioRxiv - Immunology 2019Quote: Annealed oligonucleotides containing the sequence of the HCV 5’ppp poly-U/UC region (Table 1) (20) were in vitro transcribed using the Megashortscript T7 transcription kit (Ambion) followed by ethanol precipitation ...
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bioRxiv - Cell Biology 2019Quote: ... using 0.5 mM IPTG in 2xYT broth 5 hrs at 37°C and purified under native condition over Ni-NTA Agarose (Invitrogen). Polyclonal antiserum was generated by immunization of a guinea pig (Covance ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... Fragment analysis was performed on an ABI-3730 × l sequencer using 0.5 μL PCR product combined with 15 μL HiDi Formamide and 0.15 μL LIZ 500 internal size standard (Applied Biosystems). Allele sizes were called using GeneMarker (SoftGenetics LLC ...
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bioRxiv - Plant Biology 2019Quote: ... cDNA was synthesized from 5 µg RNA using Super Script® II Reverse Transcriptase in a 20 µl system (Invitrogen). Quantitative RT-PCR was performed on a CFX384 Touch™ Real-Time PCR Detection System using SYBR Green dye (iQ™ SYBR® Green Supermix ...
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bioRxiv - Genetics 2020Quote: ... and 2 μg of plasmid encoding tethered NHA-protein using 5 μl of Lipofectamine 2000 and OPTI-MEM media (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Genetics 2021Quote: ... 100 µL of the cell suspension were then placed in a cytofunnel and spun at 1200 rpm for 5 min with high acceleration using a cytocentrifuge (Shandon Cytospin 4; ThermoFisher). For FISH ...
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bioRxiv - Cell Biology 2019Quote: ... samples were washed thrice with PBS having 0.3% Triton X-100 (PBTX) and blocked using 5% Fetal Bovine Serum (ThermoFisher Scientific) in PBTX for 2 hrs at RT ...
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bioRxiv - Cell Biology 2021Quote: ... Ten percent of the remaining lysate was reserved for input for surface levels and the rest was diluted 5-fold in lysis buffer and mixed with 50 ul of streptavidin-agarose (high-capacity, ThermoFisher). After rotation at 4°C for 2h ...
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bioRxiv - Plant Biology 2020Quote: ... The CACC at the 5’ end of the forward primer allowed directional cloning into pENTR/D-TOPO (ThermoFisher Scientific, USA) and subsequent Gateway cloning into the pSC110 expression vector ...
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bioRxiv - Plant Biology 2020Quote: ... The sections were then exposed to gold conjugated protein A of 5 nm in diameter (Zymed, now part of the Invitrogen). Sections from uninfected cassava leaves were used as additional controls in the immunolabelling experiments ...
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bioRxiv - Microbiology 2021Quote: ... cells were stimulated by infection with SeV or transfection with 5 µg/mL of poly I:C using lipofectamine 2000 (Life Technologies). RNA was extracted using the RNeasy kit (QIAGEN ...
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bioRxiv - Microbiology 2021Quote: ... cells were seeded in 6-well plates and 24 h later cells were stimulated by infection with SeV or transfection with 5 µg/mL of poly I:C using lipofectamine 2000 (Life Technologies). After stimulation ...
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bioRxiv - Microbiology 2021Quote: ... Each qPCR reaction was performed in triplicates on an ABI QuantStudio 5 PCR system (Applied Biosystems, Foster City, CA, USA). The DNA enteric viruses (AdV and crAssphage ...
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bioRxiv - Microbiology 2021Quote: ... Samples were heated to 50°C for 5 minutes before being loaded on 4-12% Novex WedgeWell Tris-Glycine gels (ThermoFisher).
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bioRxiv - Microbiology 2021Quote: ... Samples were heated to 50°C for 5 minutes before being loaded on 12% Novex WedgeWell Tris-Glycine gels (ThermoFisher). Protein bands were stained with an InstantBlue Coomassie solution (Abcam ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and TZM-bl (NIH AIDS Reagent Resource: 8129) and any derivatives produced in this study were cultivated at 37°C and 5% C02 in DMEM (Gibco) complemented with 10% fetal bovine serum (Hyclone ...
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bioRxiv - Neuroscience 2021Quote: ... neurons facing down, onto dishes containing the feeder layer of glia and 5 ml of culturing medium (94% Neurobasal, 2% Glutamax (Invitrogen), 2% B27 ...
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bioRxiv - Systems Biology 2020Quote: ... sonicated for 5 min and infused at 10 µL/min into a Thermo Q Exactive Plus spectrometer (Thermo Fisher Scientific) equipped with the HESI II ion source for shotgun lipidomics ...
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bioRxiv - Microbiology 2021Quote: Approximately 200 ng of extracted vRNA was reverse transcribed using a universal 3′ primer (5′-AGGGCTCTTCGGCCAGCRAAAGCAGG) and Superscript III reverse transcriptase (RT) (Invitrogen). The RT product was diluted approximately 10,000-fold and used as a template for quantitative PCR (qPCR) ...
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bioRxiv - Microbiology 2021Quote: Plasmodium falciparum blood stage parasites were maintained at 2% hematocrit in 1640 RPMI-HEPES supplemented with 5% AlbuMAX II (GIBCO) and 0.25% gentamycin ...