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Citations for Thermo Fisher :
9351 - 9400 of 10000+ citations for 5 Nitrofluorescein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... The wells were then washed 5 times with phosphate-buffered saline (PBS) + 0.05% Tween 20 and blocked with SEA BLOCK blocking buffer (Thermo Scientific) in the first panning round and 5% milk powder in PBS in the second panning round ...
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bioRxiv - Molecular Biology 2022Quote: ... with FLAG sequence flanking at 5’ end of the primer and cloned in Hind III and Xba I site of pCDNA3.1V5-His (Invitrogen, USA). Clones were sequenced with T7 and BGH primer using ABI BigDyeTerminator V3.1 cycle sequencing kit followed by automated sequencing in ABI 3130 Genetic Analyzer according to manufacturer’s protocol.
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bioRxiv - Microbiology 2019Quote: ... Samples were centrifuged for 5 min at 4,000 x g and suspended in ProLong™ Live Antifade Reagent (ThermoFisher Scientific). For imaging ...
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bioRxiv - Microbiology 2020Quote: ... trapped and washed on a precolumn (C18 PepMap 100, 5µm, 300 µm i.d. x 5 mm, 100 Å, Thermo Scientific) for 3 min at a flow rate of 30 μL/min with 100% buffer A (0.1% formic acid in HPLC grade water) ...
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bioRxiv - Cancer Biology 2020Quote: 1 μg total RNA from healthy and OS samples was used for 5’ and 3’ RACE reactions with the FirstChoice RLM-RACE kit (Ambion) according to manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: MDA-MB-231 cells were plated for 1 to 5 hrs on Oregon Green 488 (OR488) or AF594-conjugated cross-linked gelatin (Invitrogen) in EBSS or CM medium in the presence or absence of rapamycin as previously described 38 ...
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bioRxiv - Cancer Biology 2020Quote: ... Total RNA was isolated from sorted 2-3×10^5 CD34+ cells using the mirVANA miRNA isolation kit (Thermo Fisher) and subsequently processed using the Small RNA Library Prep kit (Norgen Biotek) ...
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bioRxiv - Cancer Biology 2020Quote: ... 95% and 100% 2x) and in two changes of xylene 5 minutes each and finally mounted using xylene-based DPX mounting media (ThermoFisher). Slides were analysed using optical microscopy ...
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bioRxiv - Cancer Biology 2020Quote: Gene-specific knockdown was achieved by reverse-transfection of PCa cell suspensions (total 5×105 cells) with 12.5 nM siRNA in 6 well plates using RNAiMAX transfection reagent (Life Technologies; Thermo Fisher Scientific ...
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bioRxiv - Genomics 2019Quote: ... and synovial sarcoma cell line (HS-SYII) were cultured in 5% CO2 in a humidified atmosphere at 37 °C in Dulbecco’s modified medium (DMEM) (Thermo Fisher) supplemented with 10 % fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2021Quote: ... so that 50 μg of total protein could be mixed with 5% Coomassie brilliant blue G-250 before loading on a 3-12% Bis-Tris BNE gel (Invitrogen). After electrophoresis (2.5 hr ...
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bioRxiv - Cell Biology 2021Quote: ... patens cDNA using a 5’ CACC sequence on the specific primers for oriented cloning into the pENTR-D-TOPO vector (Invitrogen). LR clonase (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... the cells were then washed twice with ice-cold PBS and stained nuclei with Hoechst 33342 (5 µg/mL, Invitrogen) for 10 min ...
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bioRxiv - Cell Biology 2021Quote: Full length human NHSL1d cDNA including exon 1d and excluding exon 5 was generated by cloning overlapping EST clones Flj35425 and DKFZp686P1949 into pENTR3C (Invitrogen). Fragments of NHSL1 (Fig ...
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bioRxiv - Cell Biology 2021Quote: ... cells were washed three times with imaging buffer and incubated in imaging buffer containing EGTA-AM (5 μM, Invitrogen #E1219). After 45 minutes incubation ...
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bioRxiv - Biophysics 2020Quote: ... for 5 min at room temperature and washed twice with phosphate buffered solution (1×, pH 7.4, Gibco, Thermo Fisher Scientific). The microchips were then coated with fibronectin (15 μg/mL ...
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bioRxiv - Biophysics 2020Quote: ... for 5 min at room temperature and washed twice with phosphate buffered solution (1×, pH 7.4, Gibco, Thermo Fisher Scientific). The microchips were then coated with fibronectin (15 μg/mL ...
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bioRxiv - Cell Biology 2020Quote: ... qRT-PCR was carried out in technical duplicates in 384-well plates on a QuantStudio 5 instrument (Thermo Fisher Scientific) using 2x Perfecta ROX,UNG Fast Mix (Quanta Biosciences ...
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bioRxiv - Neuroscience 2020Quote: ... HA-AP2M1 and eGFP were co-transfected at a plasmid ratio of 10:10:1 into neurons at DIV 5 using Lipofectamine 2000 reagent (Invitrogen). At 48 hours post-transfection ...
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bioRxiv - Neuroscience 2020Quote: ... the specimens were washed 2 × 5 min in PBS and mounted in ProLong® Gold antifade reagent (Life Technologies, P36934). Antibodies used for immunohistochemistry and their working concentrations are listed in Table 1.
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bioRxiv - Cell Biology 2020Quote: ... 5-ethynyl-2-deoxyuridine (EdU) labelling performed with Invitrogen Click-iT EdU Alexa Fluor 594 Imaging Kit (Life Technologies, C10339).
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bioRxiv - Biophysics 2020Quote: ... Nile Red (AC415711000), DiI-C18(5) (D3911), carboxylate-modified microspheres (0.1 μm, 350/440 blue fluorescent, F8797) were purchased from Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2021Quote: ... slides were rinsed in PBS for 3 x 5 mins and stained with 1 %g/mL 4,6-diamino-2-phenylindole (DAPI - Molecular Probes) for 3 mins in the dark at RT ...
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bioRxiv - Biophysics 2020Quote: RPE1 (ATCC) cells were incubated at 37°C in 5% CO2 in high glucose Dulbecco’s modified eagle media (DMEM) (Life Technologies), supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Biophysics 2020Quote: ... The gel was run following manufacturer instructions with “light blue” cathode buffer (0.5% NativePAGE™ Cathode Buffer Additive 20x, 5% NativePAGE™ Running Buffer 20x, 94.5% H2O) (Invitrogen) at 150 V followed by Coomassie R-250 staining ...
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bioRxiv - Biophysics 2020Quote: ... Cells were grown in an incubation chamber providing 37 °C and 5% CO2 in growth medium (DMEM, catalog no. 11880-02, Gibco) containing 1× MEM NEAA (catalog no ...
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bioRxiv - Cancer Biology 2021Quote: ... slides were incubated with spectral DAPI for 5 minutes and mounted with ProLong Gold antifade reagent (Life Technologies, cat #P36980). Negative controls included incubation with PBS solution instead of the primary antibody ...
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bioRxiv - Cancer Biology 2021Quote: PC-9 and HCC827 cell lines were cultured at 37C and 5% CO2 in RPMI 1640 GlutaMAX media (Gibco, US) supplemented with 10% fetal bovine serum ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were trypsinized and treated 30 minutes at 37°C with 5 µM CellROX Deep Red reagent (Life technologies, C10422) diluted in medium ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were permeabilised in 0.2% triton for 5 min and incubated with Click-it reaction cocktail (Click-it Alexa Fluor 647 C-10424 Invitrogen) for 30 min ...
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bioRxiv - Cell Biology 2021Quote: ... cells were incubated at 37 °C with 5% CO2 with Alexa Fluor 488–conjugated transferrin (25 μg/mL, ThermoFisher Scientific) for 30 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... and suspension diluted 1/5 by adding FACS staining buffer (SB; 2% heat-inactivated Fetal bovine serum (Life Technologies; 26140079), 25 mM HEPES (Life Technologies ...
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bioRxiv - Cell Biology 2021Quote: ... 5% CO2 in minimal medium containing 1 part DMEM (4.5 g/L D-Glucose, Pyruvate, L-Glutamine; Invitrogen 11971-025): 1 part DMEM/F12 ...
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bioRxiv - Cell Biology 2020Quote: ... HEK 293T cells were seeded at 5×10^6 cells and grown to 70-90% confluence in 10 cm plates in DMEM (Gibco) with 10% (v/v ...
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bioRxiv - Cell Biology 2020Quote: ... slides were washed 3 times in PBST for 5 minutes each and mounted using ProLong Diamond Antifade Mountant (Invitrogen, P3696). Coverslips were carefully placed over the sections ...
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bioRxiv - Cell Biology 2020Quote: ... resuspended in a final volume of 500 μl of 5% FBS in PBS and subsequently filtered into polypropylene FACS tubes (ThermoFisher). For scRNA-Seq experiments ...
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bioRxiv - Cell Biology 2020Quote: ... CD140a-PE (10 μl/106 cells, 558002, BDBioscience, Eysins, Switzerland) and CD140b-APC (5 μl/106 cells, A15719, Thermo Fisher). Right before analysis ...
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bioRxiv - Cell Biology 2020Quote: ... Peptides were loaded onto a trap column trapping cartridge (Acclaim PepMap C18 100Å, 5 mm × 300 μm i.d., #160454, Thermo Scientific) and separated by reversed-phase chromatography on a 50 cm μPAC C18 column (PharmaFluidics ...
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bioRxiv - Cell Biology 2021Quote: ... neurons were cultured at 37 °C with 5% CO2 and maintained in either maintenance media (Neurobasal (Gibco®, 21103-049) supplemented with 33 mM glucose (Corning ...
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bioRxiv - Cell Biology 2021Quote: ... THP-1 was cultured in RPMI-1640 medium supplemented with 10% heat-inactivated FBS and 5% Penstrep (Invitrogen and Himedia) in an incubator fixed at 37°C and equipped with 5% CO2 supply ...
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bioRxiv - Cell Biology 2021Quote: ... 1 µg of plasmid was used to transfect 400,000 cells in each well of a 6-well plate using Lipofectamine 2000 (5 µL; Thermo Fisher) in OptiMEM (400 µL ...
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bioRxiv - Cell Biology 2021Quote: ... samples were boiled for 5 minutes in NuPage LDS sample buffer and run on NuPage Bis-Tris 4-12% protein gels (Invitrogen), and proteins were transferred to PVDF membranes (Immobilon-FL ...
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bioRxiv - Cell Biology 2021Quote: ... The retinal sections were rehydrated with PBS and blocked with PBS containing 5% (v/v) goat serum (Thermo Fisher Scientific) and 0.1% (v/v ...
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bioRxiv - Cell Biology 2020Quote: Parent CHO-K1 and CHO-K1 cells stably expressing extracellular ACP-tagged human insulin receptors were maintained at 37°C with 5% CO2 in HAM’s F-12 medium (Thermo Fisher) supplemented 10% fetal bovine serum (FBS) ...
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bioRxiv - Cancer Biology 2020Quote: ... Labeled probes were denatured by heat for 5 min at 90 °C and then incubated with the blot in ULTRAhyp Ultrasensitive Hybridization Buffer (Invitrogen) overnight at 42 °C ...
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bioRxiv - Plant Biology 2020Quote: ... anthers were stained for a week at room temperature with 1 mM Calcofluor white for imaging of cell walls and 5 μM Syto13 (ThermoFisher) for imaging nuclei ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were cross-linked for 15 minutes at room temperature with 1% formaldehyde and quenched for 5 mins at room temperature with 0.2 M glycine (Thermo Fisher). The cross-linked cells were aliquot (~ 3X106 cells ...
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bioRxiv - Cancer Biology 2021Quote: ... Each sample was measured in technical and biological triplicate on a QuantStudio 5 Real-Time PCR machine (Thermo Fisher Scientific). Manual inspection of melting curves was performed to confirm PCR specificity ...
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bioRxiv - Cell Biology 2021Quote: ... Crypt fractions were centrifuged at 300g for 5 minutes and resuspended with ice-cold basal culture medium (Advanced DMEM/F12 (Gibco) supplemented with 2 mM GlutaMax (Gibco) ...
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bioRxiv - Cell Biology 2022Quote: ... Different concentrations of acrylamide and bis-acrylamide were mixed to produce gels of different rigidity 5 and mixed together with 2% v/v fluorescent carboxylated 200 nm beads (Invitrogen), 0.05% APS (A3678 ...