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Citations for Thermo Fisher :
9301 - 9350 of 10000+ citations for 5 NITROFURFURYL ALCOHOL since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... cDNA was synthesized from 5 ng of total RNA using TaqMan® Small RNA Assays RT-primers (Thermo Fisher Scientific) with the High-Capacity cDNA Reverse Transcription Kit ...
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bioRxiv - Cell Biology 2022Quote: ... 1 μg of plasmid was used to transfect 750,000 cells in each well of a 6-well plate using Lipofectamine 2000 (5 μL; Thermo Fisher) in OptiMEM (400 μL ...
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bioRxiv - Immunology 2022Quote: ... HEK293 cells stably transfected with human CXCR4 (HEK293- CXCR4) were used at passage 5 and were cultivated in DMEM medium (Gibco), supplemented with 10% FCS and 1% penicillin/streptomycin (Gibco) ...
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bioRxiv - Immunology 2022Quote: Annealed oligonucleotides containing the sequence of the HCV 5’ppp poly-U/UC region (34) were in vitro transcribed using the MEGAshortscript T7 transcription kit (Ambion) followed by ethanol precipitation ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... XLK-WG cells were cultured as directed by the supplier at 29°C/5% CO2 in RPMI-1640 medium supplemented with 20% fetal bovine serum (FBS; ThermoFisher) and passaged at ∼70% confluence using a 0.25% trypsin EDTA solution in 1X PBS ...
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bioRxiv - Genomics 2022Quote: ... as a monolayer at 37°C in a humidified atmosphere with 5% CO2 using Iscove’s Modified Dulbecco’s Medium (IMDM) (Life Technologies, CA) supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Cancer Biology 2022Quote: ... Cellular membranes were labeled with 5 μg/ml wheat germ agglutinin (WGA) conjugated to Alexa Fluor 488 (Thermo Fisher Scientific) for 10 min at room temperature ...
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bioRxiv - Physiology 2022Quote: ... Coverslips were subsequently washed in quadruplicate (5 min each) with PBS and diluted secondary antibody (1:500 goat anti-rat IgG Alexa Fluor 488 conjugate, Invitrogen) was applied as above ...
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bioRxiv - Cell Biology 2022Quote: ... Infection was performed in the 24-well plates and cells were subsequently selected for 5 days with Geneticin (ThermoFisher Scientific) at 75 μg/mL ...
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bioRxiv - Microbiology 2022Quote: THP-1 monocytes from the Duke Cell Culture Facility were cultured at 37°C in 5% CO2 in RPMI 1650 media (Invitrogen) supplemented with 10% heat-inactivated FBS ...
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bioRxiv - Neuroscience 2022Quote: ... Recordings were made with patch pipettes (3-5 MΩ) containing aCSF and 10 µM alexa-594 (Thermofisher, Waltham, MA, USA) and CSFcNs were targeted under visual guidance using their fluorescence ...
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bioRxiv - Neuroscience 2021Quote: ... We washed the cells 3 times for 5 minutes each with 1X PBS and mounted with ProLong Gold Antifade Mountant with DAPI media (Invitrogen) to preserve the cells for imaging ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were rinsed in Milli-Q water for 3 × 5 minutes and cover slipped with ProLong Gold Antifade (Invitrogen, P36930).
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bioRxiv - Neuroscience 2021Quote: NIH-3T3 cells were seeded on 6-well plates and allowed to grow to 70% confluency at 37°C and 5% CO2 in DMEM (Gibco) supplemented with 10% FBS (Life Technologies) ...
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bioRxiv - Neuroscience 2021Quote: ... Another 4x 15 min wash with PBST was followed by a 5 min incubation with Hoechst 33342 (Thermo Fisher Scientific), a 10 min wash with PBS and a 5 min wash with distilled water ...
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bioRxiv - Neuroscience 2021Quote: ... Brains were then washed 4X in PBS with .5% Triton X-100 (PBS-T) and then placed in blocking solution (PBS-T with 5% normal goat serum (NGS; Invitrogen)) for 90 minutes at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were washed three times with 1X PBS for 5 minutes each and blocked for 30 minutes at RT with Blocking Buffer [0.4% acetylated BSA (Ambion, AM2614) in 4X SSC] in a humidified chamber ...
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bioRxiv - Molecular Biology 2021Quote: ... was added to the cells in half culturing volume (40 uL added) and incubated in the dark for 5 min prior to reading on a Varioskan LUX plate reader (ThermoFisher). Measurements were done on all mAbs and human serum samples from each group in at least duplicate ...
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bioRxiv - Molecular Biology 2020Quote: ... One million live cells were plated in 5 ml complete Neurobasal culture medium (Neurobasal™ Medium (Gibco, cat. no. 21103049), 1x B-27 supplement (Gibco ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then washed three times for 5 mins each and incubated with fluorescently-labeled secondary antibodies (Invitrogen, Carlsbad, CA) in blocking solution for 1 h at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... Tet-on 3’ UTR HP and 5’ UTR HP DG NSCs were brought in suspension by incubating with 0.25% trypsin (Gibco #15090) in Versene (Gibco #15040 ...
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bioRxiv - Molecular Biology 2020Quote: ... and incubated with Super Signal Chemiluminescent Substrate (Thermo 34580) for 5 min with gentle rotation and imaged by iBright FL1000 (Invitrogen).
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bioRxiv - Molecular Biology 2020Quote: ... produced from DKO piglet were prepared and stained at 4°C for 45 minutes with Alexa 488-conjugated Griffonia simplicfolia isolectin IB4 (GS-IB4) (5 μg/mL, Invitrogen). The stained cells were washed twice and then analyzed by flow cytometer (BD Accuri™ C6 Plus) ...
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bioRxiv - Molecular Biology 2020Quote: ... tissue sections were deparaffinized and rehydrated before being digested with 200 μl of 5 μg/ml Proteinase K (Invitrogen, USA) in TNE buffer for 1 h at 37°C ...
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bioRxiv - Molecular Biology 2020Quote: ... 5’TYE563-labeled locked nucleic acid probes (Exiqon) against mouse major satellite sequences (Supplementary Table 5) were precipitated with mouse Cot-1 DNA (Invitrogen) and Salmon Sperm DNA (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples were boiled at 95°C for 5 min before running on a NuPAGE 4-12% Bis-Tris protein gradient mini gel (Invitrogen) at 200 V for 5 min in MOPS running buffer (Invitrogen ...
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bioRxiv - Molecular Biology 2021Quote: ... acetonitrile/water (95/5) with 10 mM ammonium acetate (pH 8.2) (All solvents were LC–MS Optima grade from Fisher Scientific). The total run time was 11 min ...
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bioRxiv - Molecular Biology 2021Quote: HEK-293 cells used in the endogenous UPF1LL knockdown and RNA-seq experiments were received from ATCC (CRL-3216) and maintained at 37°C and 5% CO2 in DMEM with 10% FBS (Gibco) and 1% pen/strep ...
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bioRxiv - Molecular Biology 2021Quote: ... in Minimum essential medium (MEM), glutamine-free based Neuronal growth medium (5% fetal bovine serum, 2% B27, 1% Glutamax (100×, Invitrogen), 2 % 1 mol/L d-glucose ...
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bioRxiv - Molecular Biology 2021Quote: Total RNA was isolated from Hela cells (n = 3) subjected to EBSS-induced autophagy and treatment with 5 μM UNC0638 for 12 hours using TRIzol reagent (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: HEK293T cells were grown at 37 °C and 5 % CO2 in DMEM supplemented with 10% tetracycline-free fetal bovine serum and 1% Penicillin-Streptomycin (Invitrogen). Cells were transfected with the LentiX VSV-G transfection mix (Clontech ...
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bioRxiv - Molecular Biology 2021Quote: ... TK6 and MCL-5 cells (Crespi et al. 1991) were cultured in suspension in 1x RPMI 1640 medium with L-glutamine (Invitrogen) supplemented with 10% horse serum (Invitrogen ...
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bioRxiv - Molecular Biology 2021Quote: ... We labeled the purified RLC with 10 molar excess of 5-((((2-Iodoacetyl)amino)ethyl)amino)naphthalene-1-sulfonic acid (IAEDANS, Invitrogen) or dabcyl C2 maleimide (AnaSpec ...
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bioRxiv - Molecular Biology 2021Quote: ... were used for each luciferase and two reference genes were also quantified with qRT-PCR using an ABI QuantStudio 5 Real-Time PCR System (ThermoFisher). Three technical replicate reactions were performed for each unique primer set for each sample.
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bioRxiv - Molecular Biology 2021Quote: J1 mES cells were grown on glass coverslips and incubated for 1h (37°C, 5% CO2) with EU from the Click-iT RNA Alexa Fluor 594 imaging kit (C10330, Invitrogen). Cells were fixed with 3,7% formaldehyde in PBS 1× for 15min at room temperature and permeabilized with 0.5% Triton X-100 in PBS 1× for 15min at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... For RNA-extraction: Three replicates of 10 (Oil and Nic) or 5 (Phe) pair of eyes were either added to RNAlater (Invitrogen) (Oil ...
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bioRxiv - Molecular Biology 2019Quote: ... 200 ul of sample was taken out and 20 μl of 5 M NaCl and 10 μl of 10 mg/ml proteinase K (Invitrogen) was then added followed by an overnight incubation on 65°C ...
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bioRxiv - Biophysics 2022Quote: ... the cells were resuspended at 5-10×106 cells per ml in HBSS without Ca2+ and Mg2+ (Thermo Fisher Scientific) to ensure their quiescence prior to stimulation by contact with IgG.
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bioRxiv - Biophysics 2022Quote: Isolated carboxysomes were diluted to 5 mg mL-1 and denatured using 4X Bromophenol blue buffer (Fisher Scientific, United States). The samples were heated at 95°C for 10 mins ...
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bioRxiv - Cell Biology 2022Quote: ... 3x105 cells were spun down at 200 g for 5 min and resuspended in 1 mL L-15 media (Gibco) containing 1μL SiR-tubulin (Cytoskeleton Inc. ...
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bioRxiv - Cell Biology 2022Quote: 100,000 BHK cells were plated into a 6-well plate and transfected with 5 nM of siRNA pool (siTOOL) against Uhrf1 or Usp7 by Lipofectamine 3000 (Invitrogen). After 24 or 48 hours ...
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bioRxiv - Cell Biology 2022Quote: ... Lysates were boiled at 95 °C for 5 min and separated by SDS–PAGE electrophoresis using 8-16% Tris-Glycine gels (Invitrogen) at 225 V for 30 min ...
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bioRxiv - Immunology 2022Quote: ... and under a 12-hour day/night photoperiod schedule (Percival Scientific, IA, USA) with 5% dextrose (w/v) and 0.05% para-Aminobenzoic acid (w/v) (Thermo Fisher). Mosquito oocysts were checked at 10-days post-infection ...
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bioRxiv - Immunology 2021Quote: ... 1% PenStrep and 8 μg/mL puromycin (to ensure retention of TMPRSS2) with 5% CO2 in a 37°C incubator (ThermoFisher). Cells were trypsinized using 0.05% trypsin and plated to 40,000 cells/well ...
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bioRxiv - Genomics 2019Quote: ... Soluble material was incubated with 3-5 μg of antibody bound to 50 μl protein A or protein G Dynabeads (Invitrogen) and incubated overnight at 4 °C ...
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bioRxiv - Genomics 2020Quote: ... Cells were washed three times with 0.01% Triton X-100 in PBS for 5 minutes each and then incubated in blocking solution containing corresponding secondary antibodies labeled with Alexa fluorophores (Invitrogen) for 1 hour at room temperature ...
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bioRxiv - Genomics 2020Quote: ... qPCR was performed in duplicates in 384-well optical plates in a QuantStudio 5 Real-Time PCR system (ThermoFisher Scientific) using default settings ...
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bioRxiv - Genomics 2020Quote: ... RNA were converted to cDNA by adding a 5-μl enzyme mix containing 500ng Actinomycin D (A7592, Thermo Fisher Scientific), 0.5μl RNase inhibitor ...
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bioRxiv - Immunology 2021Quote: ... RNA samples were first incubated at 65°C for 5 min in the presence of 0.5 mM dNTPs and 2.5 µM oligo(dT)20 (Life Technologies-Invitrogen). Subsequently ...
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bioRxiv - Genomics 2020Quote: ... and either Alexa-Fluor 594-coupled Wheat Germ Agglutinin (WGA) for WT cells or Alexa-Fluor 647-coupled WGA for Fmr1-KO cells (5 μg/ml; Invitrogen) in D-PBS for 20 and 10 min ...