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Citations for Thermo Fisher :
9301 - 9350 of 10000+ citations for 5 Nitrofluorescein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Cells were cultured at 37 C in 5% CO2 in growth media consisting of high-glucose DMEM (Gibco No. 10566), 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2021Quote: ... sections were washed at least 3-5 times with TBSTX buffer and incubated with the appropriate Alexa fluorescent secondary antibodies (Invitrogen). Sections were washed 3-5 times with TBSTX buffer and then mounted on Superfrost slides (Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were pelleted down at 2,500 rpm for 3 mins and were washed 5 times with ice-cold DPBS (Gibco #14200075). The pellets were finally dissolved in DPBS and were counted by haemocytometer using Trypan Blue stain (Gibco #15250061) ...
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bioRxiv - Cell Biology 2021Quote: ... were triple-plated in 12-well plates at a concentration of 5×104 cells/well and cultured with osteogenic differentiation medium (Gibco; StemPro ® Osteogenesis Differentiation Kit) ...
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bioRxiv - Cell Biology 2021Quote: ... were triple-plated in 12-well plates at a concentration of 5×104 cells/well and cultured with adipogenic differentiation medium (Gibco; StemPro ® Adipogenesis Differentiation Kit) ...
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bioRxiv - Microbiology 2020Quote: Purified RNA of 10 μg was denatured at 95°C for 5 min in gel loading buffer II (#AM8547, Invitrogen) and separated by 6% polyacrylamide gel with 7M urea at 300 V for 2 h ...
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bioRxiv - Microbiology 2020Quote: ... the extracted RNA (5 μL) from each tissue of individual cases was reverse transcribed using the SuperScript IV VILO cDNA synthesis system (Invitrogen). The cDNA generated from each sampled tissue (2 μL ...
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bioRxiv - Systems Biology 2021Quote: ... against Tfap4 or a negative control siPOOL at a final concentration of 5 nM using the Lipofectamine RNAiMax transfection reagent (Invitrogen), according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... denatured at 65°C for 5 min and treated with 10 U RNase-free DNase I (ThermoFisher Scientific, Waltham, USA) in DNase 1 buffer at 37 °C for 40 min ...
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bioRxiv - Microbiology 2020Quote: ... supplemented with 5% foetal calf serum (FCS) and antibiotics (100 units/mL penicillin, 100 mg/mL streptomycin, GIBCO, Life Technologies). KC cells (A Culicoides insect derived cell line ...
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bioRxiv - Microbiology 2020Quote: ... supplemented with 5% foetal calf serum (FCS) and antibiotics (100 units/mL penicillin, 100 mg/mL streptomycin, GIBCO, Life Technologies). KC cells (A Culicoides insect derived cell line ...
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bioRxiv - Microbiology 2020Quote: ... Aag2 cells were grown at 30 °C with 5% CO2 in DMEM high glucose media supplemented with 10% heat-inactivated fetal bovine serum (Gibco), 1% penicillin-streptomycin ...
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bioRxiv - Synthetic Biology 2021Quote: ... Annealing reaction was set up by mixing 1.5 µL of each oligonucleotide (100 fM) with 5 µL T4-DNA ligase buffer (Thermo Scientific) in a total reaction volume of 50 µL ...
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bioRxiv - Systems Biology 2021Quote: ... and samples were centrifuged at 16,000g for 5 minutes at −4°C in a Sorvall Legend Micro 17R microcentrifuge (Thermo Scientific). The supernatant (extract ...
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bioRxiv - Systems Biology 2021Quote: Metabolites were detected and quantified as area under the curve based on retention time and accurate mass (≤ 5 ppm) using the TraceFinder 3.3 (Thermo Scientific) software ...
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bioRxiv - Systems Biology 2021Quote: ... were cultured in a humidified incubator with 5% CO2 at 37°C in R10% medium: RPMI-1640 Medium (with glutamine, no phenol red, Gibco) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Systems Biology 2020Quote: ... The samples were then further post-fixed with 1.5 mM BS(PEG)5 (PEGylated bis(sulfosuccinimidyl)suberate) (Thermo Scientific A35396) in 1× PBS at room temperature for 20 minutes ...
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bioRxiv - Neuroscience 2021Quote: ... were cultured at 37°C in an atmosphere of 5% CO2 in Dulbecco’s modified Eagle’s medium (DMEM; Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2020Quote: ... the cultured cells were diluted 1:500 by adding 20 μL of the cell suspension to a flask containing 10 mL RNased NB and 10 μL 5-EU (100 mM stock solution; Invitrogen), giving a final concentration of 100 μM 5-EU ...
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bioRxiv - Plant Biology 2021Quote: ... the putative target of miR775 was validated by modified 5′RLM-RACE using First Choice RLM-RACE Kit (Ambion,USA) following manufacturer's instructions with minor modifications ...
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bioRxiv - Microbiology 2020Quote: RACE-PCR to determine the transcriptional start site was performed with the 5’ RACE System for Rapid Amplification of cDNA Ends (ThermoFisher) following the manufacturer protocol ...
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bioRxiv - Microbiology 2021Quote: ... amoebae transfected with vector control (pEhEx) were washed and resuspended in M199s medium and labeled with CellTracker green 5-chloromethylfluorescein diacetate (CMFDA; Invitrogen) at 186 ng/ml for 10 minutes at 35°C ...
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bioRxiv - Microbiology 2021Quote: ... Both HEK293T and A549 cell lines were maintained at 37°C and 5% CO2 in high-glucose DMEM (11-965-092, Gibco), supplemented with 10% fetal bovine serum (Genclone ...
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bioRxiv - Neuroscience 2020Quote: ... A burr hole was made to inject the fluorescent muscimol Bodipy(R)-TMR(X) (500 μM in cortex buffer with 5% DMSO, Invitrogen) in the medial part of the posterior thalamic nucleus (POm) ...
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bioRxiv - Neuroscience 2020Quote: ... Invitrogen) were applied for 5 h in antibiotic- and serum-free Opti-MEM I Reduced Serum Medium (Thermo Fisher Scientific) and cells were then grown in respective cell culture media ...
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bioRxiv - Microbiology 2021Quote: ... Transferred RNA was UV-crosslinked and hybridized overnight with 100 ng/mL of 5′ biotinylated DNA probe (Table S3) in ULTRAhyb (Ambion) hybridization buffer at 42°C ...
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bioRxiv - Neuroscience 2020Quote: Culture medium was replaced with Fluo-4 AM (5 μM) or X-Rhod-1 AM (10 μM) (both from Invitrogen) working solution in Ca2+ solution (pH 7.4) ...
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bioRxiv - Genomics 2020Quote: ... velifer embryonic fibroblasts into 6 well plates and transfected with the resulting Cas9-gRNA vector pair (5 μg DNA each, for a total of 10 μg DNA) via electroporation (ThermoFisher Neon Transfection System #MPK10025 ...
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bioRxiv - Microbiology 2021Quote: Host shutoff activity of 1918 H1N1 WT PA-X on host protein synthesis was determined by transiently transfecting HEK293T cells (5 × 104cells/well, 24-well plate format, triplicates) using Lipofectamine 2000 (LPF2000, Invitrogen) with increasing concentrations (0 ...
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bioRxiv - Neuroscience 2020Quote: ... MEF were grown as monolayers at 37°C with 5% CO2 in DMEM supplemented with 10% serum and MEM non-essential amino acids (Gibco), or with 5% delipidated fetal bovine serum and 35 μM oleic acid ...
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bioRxiv - Microbiology 2021Quote: ... RNA Stop Solution (5% phenol, 95% ethanol) was added and total RNA was extracted using TRI reagent (Thermo Fisher Scientific). RNA was treated with TURBO DNase (Thermo Fischer Scientific) ...
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bioRxiv - Cancer Biology 2021Quote: For quantification of target expression, HPAECs (Day 0, Day 5, Day 10 of chip culture) were recovered using TrypLE Express Enzyme (Gibco) at 37°C for 10 minutes ...
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bioRxiv - Immunology 2021Quote: ... The flow-through was centrifuged and resuspended in 5 ml of red blood cell lysis buffer (Invitrogen, USA, 00-4300) for 10 min ...
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bioRxiv - Immunology 2021Quote: ... forward 5’-TAACCGGTATGGGCATACTGAGCTTTCT-3’ (contains the AgeI restriction site) and reverse 5’-TAGGATCCAATCCTGTTCTGGTCGTCG-3’ (contains the BamHI restriction site) and the PCR product was cloned into pCRBlunt (Invitrogen) and sequenced ...
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bioRxiv - Biophysics 2021Quote: ... we wash the blot 3 times with TBST and soak for 5 min in chemiluminescent substrate (Thermo Scientific catalog # 34080).
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bioRxiv - Cell Biology 2020Quote: ... Samples were loaded onto a trapping cartridge (Acclaim PepMap C18 100Å, 5 mm x 300 μm i.d., 160454, Thermo Scientific) in a mobile phase of 2% ACN ...
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bioRxiv - Microbiology 2021Quote: ... using a HA-specific primer (5’-GTCCTTGCGACTG-3’) and a RevertAid first-strand cDNA synthesis kit (Invitrogen, Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... using a HA-specific primer (5’-GTCCTTGCGACTG-3’) and a RevertAid first-strand cDNA synthesis kit (Invitrogen, Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... and human hepatoma Huh7.5 cells83 were maintained at 37°C and 5% CO2 in Dulbecco’s Modified Eagle Medium (Invitrogen, Paisley, UK) supplemented with 10% heat inactivated fetal bovine serum (Sigma ...
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bioRxiv - Microbiology 2020Quote: ... An integrated loading pump was used to load peptides onto a trap column (Acclaim PepMap 100 C18, 5 um particles, 20 mm length, ThermoFisher) at 5 µL/min ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were immediately fixated by adding two times concentrated fixative (5 mM dithiobis(succinimidyl propionate) (DSP) (Thermo Fisher Scientific, USA) and 5 mM succinimidyl 3-(2-pyridyldithio)propionate (SPDP ...
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bioRxiv - Microbiology 2021Quote: ... Samples were concentrated on an Acclaim PepMap 100 (100 μm × 2 cm, nanoViper, C18, 5 μm, 100 Å; Thermo Scientific) trap column and separated on an Acclaim PepMap RSLC (75 μm × 50 cm ...
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bioRxiv - Microbiology 2021Quote: ... Membranes were washed three times for 5 min and incubated with 1:3000 dilution of horseradish peroxidase-conjugated Goat anti-mouse IgG Fc secondary antibody (Invitrogen) for 1 h ...
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bioRxiv - Plant Biology 2021Quote: ... using 5 μgr Diagenode Ab C15410004 H3K9ac antibody and a mix of 25/25 μl of protein-A/G dynabeads (Invitrogen). 5-10 ng of DNA per sample were used for library preparation with Accel-NGS 2S Plus DNA Library Kit (Swift Biosciences ...
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bioRxiv - Plant Biology 2021Quote: ... ISR1 CDS with a 5’ EcoRI restriction site and 3’ BamHI restriction site was amplified using Phusion (Thermo Fisher Scientific) and ISR1 start + EcoRI FOR and ISR1 no stop + BamHI REV primers (Table S1) ...
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bioRxiv - Microbiology 2021Quote: ... siRNA reverse transfection was performed by mixing 1.2 μl siRNA 5 μM with 1 μl lipofectamine RNAiMAX transfection reagent (Invitrogen, ThermoFisher Scientific) to a final volume of 100 μl with Opti-MEM® (Gibco ...
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bioRxiv - Cancer Biology 2019Quote: ... cells were fixed and stained for 4 h in 16% PFA solution containing 5 μg mL−1 Hoechst 33258 nuclear stain (Invitrogen). The plates were then imaged using an Operetta High Content Imaging System (PerkinElmer ...
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bioRxiv - Microbiology 2020Quote: ... and MDCK-NS1-GFP cells were maintained at 37°C and 5% CO2 in Dulbecco’s Modified Eagle Medium (DMEM, Gibco®) supplemented with 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Molecular Biology 2020Quote: ... were grown under standard conditions at 37°C at 5% CO2 in DMEM supplemented with penicillin (100 U/ml) and streptomycin (100 mg/ml) (Gibco). To investigate calcification in the GC1 cells ...
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bioRxiv - Microbiology 2020Quote: ... Cells were then washed 3 × (MBL2 wash buffer) and stained with DAPI (600 nM in MBL2 wash buffer; 5 min, RT; ThermoFisher) before imaging ...