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Citations for Thermo Fisher :
9201 - 9250 of 10000+ citations for 5 Nitrofluorescein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... COS-7 and U2OS cells were grown at 37°C with 5% CO2 in complete DMEM (Thermo Fisher Scientific, USA) containing 10% FBS and 1% L-glutamine ...
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bioRxiv - Cancer Biology 2019Quote: ... ssDNA (M13 primer ssDNA-M13 primer 5’-TGT-AAA-ACG-ACG-GCC-AGT-3’, paraformaldehyde (PFA) were purchased from ThermoFisher. PicoGreen variants were synthesized in house at ThermoFisher ...
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bioRxiv - Microbiology 2019Quote: ... burnetii total RNA extracted from LCVs (3dpi) infecting Vero (carA and metK) or THP-1 (cvpD) cells using a 5’ RACE System kit (Invitrogen) according to manufacturer protocols and with gene-specific primers (see S9 Fig) ...
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bioRxiv - Immunology 2019Quote: Annealed oligonucleotides containing the sequence of the HCV 5’ppp poly-U/UC region (Table 1) (20) were in vitro transcribed using the Megashortscript T7 transcription kit (Ambion) followed by ethanol precipitation ...
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bioRxiv - Cell Biology 2019Quote: ... using 0.5 mM IPTG in 2xYT broth 5 hrs at 37°C and purified under native condition over Ni-NTA Agarose (Invitrogen). Polyclonal antiserum was generated by immunization of a guinea pig (Covance ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... Fragment analysis was performed on an ABI-3730 × l sequencer using 0.5 μL PCR product combined with 15 μL HiDi Formamide and 0.15 μL LIZ 500 internal size standard (Applied Biosystems). Allele sizes were called using GeneMarker (SoftGenetics LLC ...
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bioRxiv - Plant Biology 2019Quote: ... cDNA was synthesized from 5 µg RNA using Super Script® II Reverse Transcriptase in a 20 µl system (Invitrogen). Quantitative RT-PCR was performed on a CFX384 Touch™ Real-Time PCR Detection System using SYBR Green dye (iQ™ SYBR® Green Supermix ...
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bioRxiv - Genetics 2020Quote: ... and 2 μg of plasmid encoding tethered NHA-protein using 5 μl of Lipofectamine 2000 and OPTI-MEM media (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Genetics 2021Quote: ... 100 µL of the cell suspension were then placed in a cytofunnel and spun at 1200 rpm for 5 min with high acceleration using a cytocentrifuge (Shandon Cytospin 4; ThermoFisher). For FISH ...
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bioRxiv - Cell Biology 2019Quote: ... samples were washed thrice with PBS having 0.3% Triton X-100 (PBTX) and blocked using 5% Fetal Bovine Serum (ThermoFisher Scientific) in PBTX for 2 hrs at RT ...
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bioRxiv - Cell Biology 2021Quote: ... Ten percent of the remaining lysate was reserved for input for surface levels and the rest was diluted 5-fold in lysis buffer and mixed with 50 ul of streptavidin-agarose (high-capacity, ThermoFisher). After rotation at 4°C for 2h ...
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bioRxiv - Plant Biology 2020Quote: ... The CACC at the 5’ end of the forward primer allowed directional cloning into pENTR/D-TOPO (ThermoFisher Scientific, USA) and subsequent Gateway cloning into the pSC110 expression vector ...
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bioRxiv - Plant Biology 2020Quote: ... The sections were then exposed to gold conjugated protein A of 5 nm in diameter (Zymed, now part of the Invitrogen). Sections from uninfected cassava leaves were used as additional controls in the immunolabelling experiments ...
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bioRxiv - Microbiology 2021Quote: ... cells were stimulated by infection with SeV or transfection with 5 µg/mL of poly I:C using lipofectamine 2000 (Life Technologies). RNA was extracted using the RNeasy kit (QIAGEN ...
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bioRxiv - Microbiology 2021Quote: ... cells were seeded in 6-well plates and 24 h later cells were stimulated by infection with SeV or transfection with 5 µg/mL of poly I:C using lipofectamine 2000 (Life Technologies). After stimulation ...
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bioRxiv - Microbiology 2021Quote: ... Each qPCR reaction was performed in triplicates on an ABI QuantStudio 5 PCR system (Applied Biosystems, Foster City, CA, USA). The DNA enteric viruses (AdV and crAssphage ...
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bioRxiv - Microbiology 2021Quote: ... Samples were heated to 50°C for 5 minutes before being loaded on 4-12% Novex WedgeWell Tris-Glycine gels (ThermoFisher).
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bioRxiv - Microbiology 2021Quote: ... Samples were heated to 50°C for 5 minutes before being loaded on 12% Novex WedgeWell Tris-Glycine gels (ThermoFisher). Protein bands were stained with an InstantBlue Coomassie solution (Abcam ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and TZM-bl (NIH AIDS Reagent Resource: 8129) and any derivatives produced in this study were cultivated at 37°C and 5% C02 in DMEM (Gibco) complemented with 10% fetal bovine serum (Hyclone ...
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bioRxiv - Neuroscience 2021Quote: ... neurons facing down, onto dishes containing the feeder layer of glia and 5 ml of culturing medium (94% Neurobasal, 2% Glutamax (Invitrogen), 2% B27 ...
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bioRxiv - Systems Biology 2020Quote: ... sonicated for 5 min and infused at 10 µL/min into a Thermo Q Exactive Plus spectrometer (Thermo Fisher Scientific) equipped with the HESI II ion source for shotgun lipidomics ...
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bioRxiv - Microbiology 2021Quote: Approximately 200 ng of extracted vRNA was reverse transcribed using a universal 3′ primer (5′-AGGGCTCTTCGGCCAGCRAAAGCAGG) and Superscript III reverse transcriptase (RT) (Invitrogen). The RT product was diluted approximately 10,000-fold and used as a template for quantitative PCR (qPCR) ...
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bioRxiv - Microbiology 2021Quote: Plasmodium falciparum blood stage parasites were maintained at 2% hematocrit in 1640 RPMI-HEPES supplemented with 5% AlbuMAX II (GIBCO) and 0.25% gentamycin ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The second round PCR reaction included 5 uL of 1X Platinum SuperFi Library Amplification Master Mix (Thermo Fisher, Waltham, Massachusetts), 5 uL of the first round PCR product ...
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bioRxiv - Neuroscience 2020Quote: ... at a density of 300 cells/mm2 and incubated at 37 °C and 5 % CO2 in a humid atmosphere in DMEM (Invitrogen) supplemented with 10% FBS ...
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bioRxiv - Neuroscience 2020Quote: ... red blood cells (RBCs) were lysed for 5 min at room temperature using ammonium-chloride-potassium (ACK) buffer (Life Technologies). After centrifugation ...
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bioRxiv - Neuroscience 2020Quote: ... HEK293T cells and HeLa cells were cultured at 37°C in 5% CO2 in DMEM (Biological Industries) supplemented with 10% (v/v) fetal bovine serum (FBS, Gibco) and 1% penicillin-streptomycin (Gibco).
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bioRxiv - Neuroscience 2020Quote: ... Cultures were kept at 37 °C and 5 % CO2 in equilibrated maintenance medium containing 96 % Neurobasal medium (Gibco, Cat.# 21103049), 2 % B-27 plus Supplement (Gibco ...
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bioRxiv - Cell Biology 2021Quote: ... The beads were then washed with 1mL of ice-cold lysis buffer four times before elution of bound material via a 5 min incubation at 98 °C with 30 μL of 2x LDS buffer (Invitrogen). About 25 μL of eluate was collected by centrifugation ...
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bioRxiv - Bioengineering 2021Quote: ... 10 µg of each sample was boiled at 95°C for 5 min and loaded onto the 4-12% Bis-Tris Gel (NP0336, ThermoFisher). Gels were run for 1 hour and 15 min at 120 V in with NuPage MES Buffer (NP0002 ...
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bioRxiv - Immunology 2020Quote: ... and before adoptive transfer thawed and rested overnight at 37°C and 5% CO2 in RPMIc (RPMI 1640 (Gibco; 31870074) with 5% human serum (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2020Quote: ... siRNAs and Gapmers or shRNAs were introduced to primary hippocampal neurons (5–8 days in vitro (DIV)) using Lipofectamine RNAiMAX or Lipofectamine 2000 (Invitrogen) according to manufacturer’s guidelines ...
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bioRxiv - Cancer Biology 2021Quote: ... for the last 30 min of the drug treatment in L-methionine-depleted medium containing 5% dialyzed fetal calf serum (FCS; Gibco). Harvested cells were washed with PBS ...
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bioRxiv - Bioengineering 2022Quote: ... using vacuum filtration (Berg and Turner, 1990) and resuspended into 5% RMB (RMB, including 9.13 g/L Na2HPO4 (Fisher Scientific), 4.87 g/L NaH2PO4·H2O (Amresco) ...
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bioRxiv - Bioengineering 2021Quote: ... Samples were analyzed using an Agilent 1100 HPLC system equipped with a MAbPac SEC-1 analytical column (4.0 × 300 mm, 5 µm, Thermo Scientific). The conditions were as follows ...
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bioRxiv - Bioengineering 2021Quote: ... The reaction was monitored using an Agilent 1100 HPLC system equipped with a MAbPac HIC-Butyl column (4.6 × 100 mm, 5 µm, Thermo Scientific). Elution conditions were as follows ...
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bioRxiv - Bioengineering 2021Quote: ... and exposed to 25 µM DRAQ5 Fluorescent Probe for 5 min at room temperature under agitation (#62254, Thermo Scientific™).
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bioRxiv - Biophysics 2021Quote: Cells were magnetically labelled thanks to an incubation of 2h with a solution of iron oxide nanoparticles at [Fe] = 4 mM and supplemented with 5 mM citrate in RPMI medium (Gibco). The viability and the proliferation of cells after magnetic labelling were assessed by Alamar Blue showing no impact of the magnetic labelling right after the labelling or after one day (Supplementary Figure 1).
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bioRxiv - Biochemistry 2020Quote: ... 5 DNA and protein concentrations were measured using a NanoDrop ND-2000 UV-visible spectrophotometer (Thermo Fisher Scientific, Nottingham, UK).
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bioRxiv - Biochemistry 2020Quote: ... After transfer and blocking of polyvinylidene fluoride (PVDF) membranes (Immobilon) in 5% BSA TBS Tween-20 buffer (Thermo Fisher Scientific), primary antibody directed against FADS2 (PA5-87765 ...
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bioRxiv - Biochemistry 2020Quote: ... was added to the cells and incubated in the dark for 5-10 minutes prior to reading on a Varioskan LUX plate reader (ThermoFisher). Measurements were done in duplicate and relative luciferase units (RLU ...
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bioRxiv - Bioengineering 2020Quote: ... The cells were incubated at 37°C in an atmosphere of 5% CO2 in air in Low-glucose Dulbecco’s Modified Eagle’s Medium (DMEM, Gibco, Invitrogen, Karlsruhe, Germany) supplemented with 10% heat inactivated Fetal Bovine Serum (FBS ...
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bioRxiv - Bioengineering 2020Quote: ... Separation was performed on an Aquasil C18 column (250 cm x 3 mm, 5 µm particle size; Thermo Fisher Scinetific) at a flow rate of 1 ml/min ...
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bioRxiv - Bioengineering 2021Quote: ... Droplets of 5 μL cell-Matrigel mixture were manually deposited onto a high performance #1.5 glass bottom 6-well plate (Fisher Scientific). Data acquisition was performed using a confocal microscope (Nikon A1R HD25 ...
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bioRxiv - Bioengineering 2021Quote: ... the tissues were washed with PBS (3X, 5 minute interval in the shaker) and goat anti-mouse Alexa 594 (A11005, Invitrogen) 1:200 dilutions was added for 3 hours in RT ...
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bioRxiv - Bioengineering 2021Quote: ... A similar procedure was used for monolayer cell culture (the cells were rinsed with PBS (3x 5 min) and detached using trypsin (Gibco)).
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bioRxiv - Bioengineering 2020Quote: ... The cells were passaged at a density of 1:2 to 1:8 after reaching ~80% confluency by detaching with 5 mM EDTA in PBS (Invitrogen 15575-038 diluted in sterile 1X PBS ...
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bioRxiv - Bioengineering 2020Quote: ... Primary fibroblast passages 2–6 were achieved in culture in 37°C in a 5% CO2 incubator with DMEM High Glucose (Gibco) with 10% FBS to 80% confluence in 100 mm Petri dishes ...
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bioRxiv - Bioengineering 2020Quote: ... Mabs were transduced by nLacZ lentiviral particles and cultured on Falcon dishes at 37°C with 5% CO2 in DMEM GlutaMAX (Gibco) supplemented with heat-inactivated 10% fetal bovine serum (FBS) ...
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bioRxiv - Bioengineering 2020Quote: ... Oligonucleotide-loaded sender cells were then stained with 5 µg/mL streptavidin-conjugated Alexa Fluor 647 (Thermo Fisher Scientific S32357) for 30 minutes on ice ...