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Citations for Thermo Fisher :
9351 - 9400 of 9427 citations for Vertical Gel Casting Cassettes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Samples were first boiled with 6x Laemmli SDS buffer at 95°C for 5 minutes and then proteins were separated on Bolt 4-12% Bis-Tris gels (Thermo Fisher, NWO4122BOX and NWO4127BOX), transferred to Nitrocellulose membranes using the Mini Trans-Blot Cell (BioRad ...
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bioRxiv - Biophysics 2023Quote: Air-dried grids were placed GrOx-side-up on a glass slide pre-cleaned with 70% EtOH and mounted beneath a coverslip in ProLong™ Gold or ProLong™ Diamond Antifade Mountant gel (Thermo Fisher Scientific).
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bioRxiv - Bioengineering 2023Quote: ... samples were then incubated at 65°C for 15 min and immediately run on NuPAGE Bis-Tris gels (Thermo Fisher Scientific, Waltham, MA, USA) as per the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... and approximately 5.0 kb of the PCR product was excised from the gel and subcloned into the pCR®4-TOPO® vector using the TOPO TA Cloning® Kit (Invitrogen, USA). The nucleotide sequences of the cDNA ends were determined by dideoxy chain termination chemistry using a Thermo Sequenase™ Cycle Sequencing Kit (USB Corporation ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the generated DNA fragment was purified from agarose gel and subcloned into a pCR™Blunt II-TOPO™ vector using Zero Blunt TOPO PCR cloning kit (ThermoFisher Scientific, USA). Afterwards ...
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bioRxiv - Biochemistry 2023Quote: ... The eluted samples were run on 15% SDS-polyacrylamide gel under reducing conditions followed by staining by silver staining solution using Pierce™ Silver Stain Kit (Thermo Fisher Scientific, USA) (Fig ...
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bioRxiv - Cell Biology 2023Quote: ... Each protein extract was boiled in 1x LDS sample buffer with 1:10 TCEP solution reducing agent and then size fractionated via pre-cast SDS-PAGE Bis-Tris 4-12% gels (Thermo Fisher Scientific, Waltham, MA), and transferred onto nitrocellulose membranes with the iBlot-2 system (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2023Quote: ... The quantity and quality of DNA were confirmed by electrophoresis on agarose gels stained with GelRed and visualized under UV light using a NanoDrop 1000 spectrophotometer (Thermo Scientific, Rockford, IL, USA). Nine samples (three from each treatment ...
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bioRxiv - Biochemistry 2023Quote: ... The samples were separated on a 15% denaturing urea-PAGE and visualized via SYBR™ Gold Nucleic Acid Gel Stain (Invitrogen by Thermo Fisher).
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bioRxiv - Cancer Biology 2023Quote: ... Approximately 10–30 µg of total protein was run on a 4–12% Bis-Tris pre-cast NuPage gel (Life Technologies, NP0321 or NP0322) on the Novex gel system and subsequently transferred onto PVDF membrane (Immobilon ...
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bioRxiv - Synthetic Biology 2023Quote: ... 1 % agarose gels were run at 100 V for 20-30 min using 6x DNA Gel Loading Dye and GeneRuler 1 kb DNA Ladder (Thermo Fisher Scientific Inc., U.S.A). Both gel purifications and PCR clean-ups were performed using the NucleoSpin® PCR and Gel Clean Up Kit (Macherey-Nagel ...
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bioRxiv - Plant Biology 2023Quote: ... The quantity and quality of DNA was examined on 1% agarose gel and Quan-iT PicoGreen DsDNA assay kit (Life Technologies, Carlsbad, CA, USA) on a FLUOstar Omega fluorometer (BMG Labtech ...
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bioRxiv - Biochemistry 2023Quote: ... Eluates carrying fluorescently labeled RPA elutes first and were analyzed on a 12% SDS-PAGE gel and imaged using a iBright 1500 imager (Invitrogen Inc.; Figure 2G & H). While all three RPA subunits are observed in the Coomassie stained image ...
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bioRxiv - Cancer Biology 2023Quote: ... Equal amounts of protein (15 to 20 μg for each lane) were separated by SDS-PAGE using Novex WedgeWell 4-20% Tris-Glycine gels (Fisher Scientific, Waltham, MA, USA) and transferred to nitrocellulose membranes (Protran 0.1 μm ...
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bioRxiv - Bioengineering 2023Quote: ... Protein was quantified and 80 μg of each lysate were loaded per lane of a NuPAGE™ 4-12% Bis-Tris Protein Gel (Thermo Fisher Scientific, NP0335PK2). Samples were separated on 200V for 35 min in the mini gel tank (ThermoFisher) ...
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bioRxiv - Plant Biology 2023Quote: ... The quality and concentration of the purified DNA samples were determined by gel electrophoresis and in the Qubit 3.0 Fluorometer (Thermo Fisher Scientific, Waltham, MA, USA). RADseq library was prepared with HindIII and NlaIII restriction enzymes for DNA digestion and sequenced using Illumina NovaSeq 6000 (Illumina ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The reactions were then loaded onto a 1% agarose gel and electrophoresed in an Owl™ D3-14 electrophoresis box (Thermo Scientific, MA, USA) containing 1X TBE buffer for 30 min at 180 V delivered from an Owl™ EC 300 XL power supply (Thermo Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... individual 3rd instar larvae were placed in a square behavior arena (20 cm x 20 cm x 1 cm) layered with a 1% agarose gel (Fisher Scientific, Loughborough, United Kingdom) to facilitate movement tracking ...
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bioRxiv - Cancer Biology 2024Quote: ... Amplified DNA products were separated by agarose gel electrophoresis and visualized by iBright CL1500 Imaging System (Invitrogen, Thermo Fisher Scientific, Inc., Waltham, MA, USA). Amplified regions of CRISPR-C unedited samples and the specific bands derived from the amplification of the deletion scar and circular junction sites were dissected and DNA products purified by NucleoSpin Gel and PCR Clean-up kit (MACHEREY-NAGEL Düren ...
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bioRxiv - Evolutionary Biology 2024Quote: ... DNA quality was checked with standard electrophoresis in 1% agarose gel and the quantity was measured using NanoDropTM and Qubit Assay (Thermo Fisher ScientificTM, Carlsbad, USA).
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bioRxiv - Microbiology 2024Quote: Five - 10mg of protein from the cell lysates were run on 4-12% NuPAGE Bis-Tris SDS-PAGE gels (Invitrogen by ThermoFisher Scientific®) and transferred to PVDF membranes (ThermoFisher Scientific® ...
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bioRxiv - Neuroscience 2024Quote: ... Equal amounts of protein (50 ug for liver, cerebellum, and cerebrum) were run on 4-12% Bis-Tris SDS-polyacrylamide gels (NP0321BOX/Invitrogen by Thermo Fisher Scientific) and separation was achieved via electrophoresis ...
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bioRxiv - Cell Biology 2024Quote: ... Protein samples were denatured at 100 °C for 5 minutes and briefly centrifuged before loading onto a 4-12% Tris-Glycine Novex Wedgewell gel (Fisher Scientific, Cat no. XP04125BOX). SDS-PAGE was performed using standard running buffer in Xcell Surelock Electrophoresis Cell (Invitrogen ...
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bioRxiv - Immunology 2024Quote: ... or 37 µg (for splenocytes) of protein was loaded per well onto NuPAGE 4–12% Bis– Tris Midi Gel (Thermo Fisher Scientific, Cat# WG1403BOX) and separated under NuPAGE MOPS SDS running buffer (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: PCR amplicons for 16S rRNA and toxin genes were analyzed by electrophoresis on 1% agarose gels using 100 bp DNA ladders (Thermo Fisher Scientific, Mumbai, India). Agrose gels were stained with ethidium bromide (10 lg/100 ml ...
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bioRxiv - Microbiology 2024Quote: ... The quality of the extracted DNA was checked using agarose gel (1.5%) electrophoresis and quantified with Quant-iT™ PicoGreen® dsDNA reagent kit (Thermo scientific, MA, USA).
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bioRxiv - Plant Biology 2020Quote: ... The resulting PCR fragment was gel purified and cloned into the gateway entry vector pENTR®/D-TOPO® (Thermo Fisher Scientific, Waltham, MA, USA). The RGO promoter ...
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bioRxiv - Genomics 2020Quote: ... and RNA integrity was estimated by separating 2 μL of each sample on a 2% agarose gel and quantified using a Nanodrop (Thermo Fisher Science, Waltham, MA, USA) and stored at −80⁰C ...
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bioRxiv - Molecular Biology 2019Quote: ... 15 µl of a 1:1 volume ratio of gliadin and eIF4A was loaded into each lane of a Novex NuPAGE 4-12% Bis-Tris gel and electrophoresed in a Bolt® Mini Gel Tank at 165 V for 1 h with MOPS SDS running buffer (Life Technologies, Frederick, MD, USA).
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bioRxiv - Developmental Biology 2019Quote: ... The amplified PCR fragments were gel purified and cloned into the pCR©II-TOPO© vector using the TOPO©TA Cloning© Dual Promoter kit (ThermoFisher Scientific) following the manufacturer’s instructions.
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bioRxiv - Plant Biology 2020Quote: ... The PCR products were analyzed in 1.5% agarose gel to confirm the targeted amplicon size and purified using GeneJET™ PCR Purification Kit (Thermo scientific™, Waltham, Massachusetts, USA) The PCR purified samples were sequenced by Sanger sequencing service provided by GENEWIZ ...
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bioRxiv - Neuroscience 2020Quote: ... or self-cast 10% Bis-Tris gel and electrophoresed at 130 V for 2 h with NuPAGE MOPS SDS running buffer (Thermo Fisher Scientific, Cat# NP0001-02). At the end of electrophoresis ...
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bioRxiv - Cancer Biology 2021Quote: 20 µg of proteins extracted in SDS buffer (1% SDS supplemented with protease and phosphatase inhibitors) were loaded on to a 4-12% gradient SDS-PAGE gel (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA) and transferred to a PVDF membrane (GE Healthcare ...
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bioRxiv - Microbiology 2020Quote: ... Protein (5 μg) samples were resolved in a 10 % SDS-PAGE gel and transferred to an Amersham™ Protran™ NC membrane (Fisher Scientific, Cat#10600005). The membrane was blocked with blocking buffer (5 % skim milk in Tris-Buffered Saline with 0.1% Tween ...
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bioRxiv - Molecular Biology 2022Quote: ... The digested vectors as well as insert fragments were gel excised and ligated in a vector to insert ratio of 1:3 using T4 DNA ligase (Thermo Scientific™, Cat no. # EL0011) as per manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... and RNA integrity was determined by separating 2 μL of each sample on a 2% agarose gel and quantified using a Nanodrop (Thermo Fisher Science, Waltham, MA, USA) and stored at -80°C until further use ...
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bioRxiv - Immunology 2020Quote: ... For each sample 10 μg of total protein lysate was used for protein separation in Precise 4–20% gradient Tris-SDS gels (Bolt, Thermo Fisher Scientific, Landsmeer, The Netherlands). Subsequently ...
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bioRxiv - Microbiology 2022Quote: ... The identity and concentration of the purified protein was examined and quantified using the sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) and the Pierce Bradford Protein Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA).
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bioRxiv - Neuroscience 2022Quote: ... or self-cast 10% Bis-Tris gels and electrophoresed at 130 V for 2 hours with NuPAGE MOPS SDS running buffer (Thermo Fisher Scientific, Cat# NP0001-02). At the end of electrophoresis ...
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bioRxiv - Genetics 2019Quote: ... The amplified PCR products were separated by electrophoresis on a 1% agarose gel along with the GeneRuler 1 kb DNA ladder Plus (Thermo Fisher Scientific, Waltham, MA, U.S.A.) as marker ...
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bioRxiv - Bioengineering 2020Quote: ... although instead of staining the gel the proteins were transferred to a nitrocellulose membrane using an iBlot Dry Blotting System (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA) at 20V for 7 minutes ...
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bioRxiv - Molecular Biology 2021Quote: ... and a gel slice containing RNA of approximately 30 nt was excised and ground by an RNase-free pellet pestle (Fisher Scientific, Cat: 12-141-364). RNA was extracted from the gel slice by adding 500 µl of 300 mM NaAc (pH = 5.5) ...
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bioRxiv - Biochemistry 2020Quote: Thin layer chromatography was performed on aluminum backed silica gel plates (median pore size 60 Å, fluorescent indicator 254 nm, Fisher Scientific, Hampton, NH, US) and visualized by exposure to UV light (365 nm ...
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bioRxiv - Genetics 2021Quote: ... Eveleigh NSW Australia) run for 40 min at 90 V stained with SYBR™ Safe DNA Gel Stain (Thermo Fisher Scientific Inc. Waltham MA USA) and viewed with a GelDoc XR Gel Documentation System (Bio-Rad Laboratories Inc ...
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bioRxiv - Evolutionary Biology 2020Quote: ... DNA was first quality-checked by gel electrophoresis and then quantified with a PicoGreen fluorometer quantitator and Quant-iT™ PicoGreen™ dsDNA Assay Kit (Invitrogen™, ThermoFisher Scientific). The Genomic Sequencing and Analysis Facility (GSAF ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were transferred onto nitrocellulose membranes using either the BioRAD Semi-Dry Blot system (30 minutes in 1xBjerrum buffer + 20 % methanol) for SDS-PAGE gels or the iBlot2® (P0, 7mins, using iBlot® 2NC regular stacks) (Invitrogen, Thermo Fisher Scientific) for pre-casted gels ...
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Fusarium solani species complex genomes reveal bases of compartmentalisation and animal pathogenesisbioRxiv - Genomics 2022Quote: ... The integrity of the isolated RNA was checked using 1.5% agarose gel and quantity was determined using Invitrogen Qubit® 4 Fluorometer (Thermo Fisher Scientific, Waltham, MA, USA) before library preparation and sequencing (Supplementary Table 25).
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bioRxiv - Microbiology 2022Quote: ... Samples were run at 150 V for 1 hr on Novex Bolt™ Bis-Tris Plus precast Gels (4-12%) with 1x MOPS buffer (Life Technologies Australia Pty Ltd) and transferred to a nitrocellulose membrane (Bio-Rad ...
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bioRxiv - Neuroscience 2022Quote: ... The beads in sample buffer were then heated at 70°C for 10 min prior to SDS-PAGE on a 4-12% gradient NuPAGE Bis/Tris gels (ThermoFisher Scientific, Waltham, MA; Cat#NP0322BOX) followed by transfer to nitrocellulose membrane with 10% methanol for 6 hours at constant 150 mA ...
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bioRxiv - Cell Biology 2024Quote: Blue Native-PAGE (BN-Page) was conducted following the protocol from NativePAGE™ Novex® Bis-Tris Gel System kit (Thermo Fisher Scientific, Massachusetts, USA). Samples separated by membrane fractionation were utilized in BN-Page ...