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Citations for Thermo Fisher :
9051 - 9100 of 9427 citations for Vertical Gel Casting Cassettes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... Proteins were separated by dodecyl-sulfate polyacrylamide gel electrophoresis and transferred to Amersham™ Protran® nitrocellulose membranes or PVDF membranes (Invitrogen). The membranes were blocked with 5% non-fat dry milk-TBST (10 mM Tris-HCL [pH 8.0] ...
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bioRxiv - Bioengineering 2024Quote: ... 1 % RGD and 5 % RGD gels were formed to volumes of 500 μL in black 96-well MicroWell plates (Nunc, Roskilde, Denmark), and incubated for 24 hours ...
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bioRxiv - Molecular Biology 2024Quote: ... The protein purity was assessed using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) (Laemmli, 1970) and staining (PageBlueTM solution from Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2024Quote: ... Samples were separated on a 3 – 12% polyacrylamide gradient gel (Serva, #43250) alongside a molecular weight marker (NativeMark, Thermo Fisher Scientific, #LC0725). After electrophoresis ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins present in supernatant were solubilized with Laemmli sample buffer and 40 µl of each sample were resolved by NuPAGE 4-12% Bis-Tris gel (MES buffer, Thermo Scientific, WG1401) and transferred onto nitrocellulose membranes ...
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bioRxiv - Neuroscience 2024Quote: ... The purity of produced viruses was routinely checked by silver staining (Pierce, Germany) after gel electrophoresis (NovexTM 4–12% Tris-Glycine, Thermo Fisher Scientific) according to the manufacturer’s instructions viral stocks were kept at −80°C until the injection day.
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bioRxiv - Genomics 2024Quote: The quality-compliant DNA was further tested for purity and integrity using 0.8% agarose gel electrophoresis and a Qubit dsDNA HS Assay Kit on a Qubit 3.0 Fluorometer (Life Technologies, Carlsbad, CA, USA). RNA contamination was identified using a 1.5% agarose gel ...
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bioRxiv - Neuroscience 2024Quote: ... Brain extracts containing 60 ug of protein were loaded and separated on 6-10% denaturing polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to polyvinylidene difluoride (PVDF) membrane (Cat#88518; Thermo Fisher Scientific). The membrane was blocked with 5% milk in Tris-buffered saline with 0.1% Tween (TBST ...
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bioRxiv - Bioengineering 2024Quote: ... Samples were then boiled at 95°C for 5 min and electrophoresed using a NuPAGE Bis-Tris Gel (4-12%) with NuPAGE SDS Running Buffer (Thermo Fisher Scientific) for 2 hr at 130 V ...
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bioRxiv - Bioengineering 2024Quote: ... Protein lysates were then electrophoresed on a NuPAGE Bis-Tris Protein Gel (4-12%) using NuPAGE SDS Running Buffer (Thermo Fisher Scientific) for 2 hr at 130 V before transfer to a nitrocellulose membrane in Towbin Buffer (20 mM Tris-HCl pH 8.3 ...
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bioRxiv - Biochemistry 2024Quote: ... Reaction products were separated on 0.7% agarose gel in TA buffer (40 mM Tris-acetate pH 8) at 4 °C and visualized by Sybr Gold staining (Thermo Fisher Scientific).
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bioRxiv - Biochemistry 2024Quote: Protein samples (20 mg of total protein per lane) were separated by SDS-PAGE using precast 10% NuPAGE Bis-Tris midi gels (Thermo Fisher Scientific). Protein was electroblotted onto PVDF (polyvinylidene fluoride ...
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bioRxiv - Plant Biology 2024Quote: ... Fifteen ul of supernatant were run on Bio-Rad 4-20% Mini-PROTEAN TGX gels with PageRuler Plus prestained protein ladder (Thermo Fisher Scientific). Proteins were transferred to PVDF difluoride membranes in Trans-Blot Turbo transfer buffer in a Trans-Blot Turbo transfer system (Bio-Rad ...
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bioRxiv - Neuroscience 2024Quote: ... denatured at 95°C for 5 min and run on 3–8% NuPAGE Tris-acetate gels in NuPAGE Tris-acetate SDS Running Buffer (20x) (Thermo Fisher Scientific) at 4°C ...
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bioRxiv - Biochemistry 2024Quote: ... 20 μg of total protein (from whole cell extracts or isolated lysosomes) was separated with a 4%–12% Bis-Tris Plus Gels (Invitrogen, Thermo Scientific) and then transferred onto PVDF (polyvinylidene difluoride ...
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bioRxiv - Biochemistry 2024Quote: ... Samples were incubated for 10 min at 95 °C and were immediately loaded in 4%–12% Bis-Tris Plus Gels (Invitrogen, Thermo Scientific) and ran at constant 120 V (see below).
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bioRxiv - Microbiology 2024Quote: ... Substrate and self-cleaved products were separated by PAGE in 10% denaturing gels and detected by Sybr Gold staining (Thermo Fisher Scientific). In both cases ...
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bioRxiv - Microbiology 2024Quote: ... the uncleaved and cleaved transcripts obtained at different times were separated by PAGE in 10% denaturing gels and detected by Sybr Gold staining (Thermo Fisher Scientific). For post-transcriptional cleavage analysis ...
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bioRxiv - Developmental Biology 2024Quote: For measuring in-gel activity the protocol was slightly modified by using 200 ml of Native PAGE anode buffer (Thermo Fisher Scientific) supplemented with 0.022 g Coomassie Brilliant Blue G-250 instead of the regular light blue cathode buffer.
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bioRxiv - Microbiology 2024Quote: ... The resulting PCR products were visualised by 1.5% agarose gel electrophoresis and subsequently purified using the ExoSAP™ PCR Product Cleanup Kit (Applied Biosystems, USA). Sanger sequencing of the 16S rRNA gene was performed at BMR Genomics Company ...
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bioRxiv - Microbiology 2024Quote: ... was estimated based on the intensity of the LPS-corresponding band from the individual samples that were ran on a polyacrylamide gel stained using silver stain kit (Thermo Fisher Scientific) and alcaine blue (Millipore Sigma ...
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bioRxiv - Molecular Biology 2024Quote: ... protein samples were resolved on a stain-free 12 % SDS-PAGE gel together with a pre-stained Page Ruler protein ladder (Thermo Fisher Scientific). SDS-PAGE gels were equilibrated for 10 min in transfer buffer (190 mM Glycine / 20 mM Tris-Base / 20 % Ethanol) ...
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bioRxiv - Molecular Biology 2024Quote: ... An additional size-fractionation step was performed for all EV and NVEP fractions using a Novex TBE PAGE gel (6%, Invitrogen, Cat# EC6265BOX) with a size range of 150 – 400 bp for cell line-derived cells and EVs ...
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bioRxiv - Neuroscience 2024Quote: ... and equal amounts of protein lysates (∼20 μg per lane) were resolved on 4–12% Bis-Tris SDS–PAGE gels and then transferred to polyvinylidene difluoride membranes (Invitrogen, Carlsbad, CA). After blocking with 5% skimmed milk ...
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bioRxiv - Immunology 2024Quote: ... the mAbs following expression were purified and subjected to SDS-PAGE analysis using a NuPAGE 4-12% Bis-Tris gel (Thermo Scientific Inc) under reducing (lane 1 ...
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bioRxiv - Molecular Biology 2024Quote: ... 25 – 50 ug of overall protein containing sample buffer and reducing buffer was loaded onto a 3 – 8% tris-acetate gel (Thermo Fisher Scientific) using tris acetate running buffer (Life Technologies) ...
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bioRxiv - Plant Biology 2024Quote: ... The amplicons obtained from this PCR reaction were gel-isolated and inserted into pHEE401E (64) using BsaI and T4 ligase (Thermo Fisher Scientific). The pHEE401E vectors containing gRNAs against AGO4 and AGO6 were transformed into the Agrobacterium tumefaciens strain GV3101 ...
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bioRxiv - Plant Biology 2020Quote: ... Cleared cell lysate was denatured with SDS buffer and separated by SDS–PAGE with Novex 4–12% Tris-glycine polyacrylamide gel (Invitrogen, Life Technologies, CA). Western blot analysis was performed using anti-mCherry antibody (16D7 ...
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bioRxiv - Microbiology 2020Quote: Deglycosylated and native samples containing GP cleavage products were analyzed by SDS-PAGE using 10% Tricine protein gels (Thermo Fisher Scientific, Waltham, MA). Samples were transferred onto 0.2 μm pore size Protran nitrocellulose membranes (Sigma-Aldrich ...
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bioRxiv - Immunology 2021Quote: ... Mice were genotyped by PCR using forward primers 5’-ctgagcagagacccactgaaag-3’ and reverse primers 5’- ggatctggcttctgagtttgtgta-3’ and amplicons were ran in 6% TBE gels (Life Technologies, Carlsbad, CA).
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bioRxiv - Microbiology 2021Quote: ... electrophoresed proteins were transferred from an acrylamide gel onto a nitrocellulose membrane using the iBlot 2 dry blotting system and transfer stack (ThermoFisher, #IB21001 and #IB23001). After transfer ...
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bioRxiv - Microbiology 2021Quote: ... The resulting cDNA was either amplified by PCR and detected by gel electrophoresis or quantified by qRT-PCR using the PowerUp SYBR green qPCR kit (Applied Biosystems, catalog # A25918).
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bioRxiv - Molecular Biology 2021Quote: ... Samples from in vitro translation reactions of hRLuc and 3xFLAG-HBB were run on 4-12% Bis-Tris NuPAGE gels (Invitrogen, Cat. No. WG1203BOX). Samples of SMG6-3xFLAG in vitro translation reactions were run on 3-8% Tris-Acetate NuPAGE gel (Invitrogen ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples to test nuclear and cytoplasmic markers and eIF2α phosphorylation were run on 4-12% Bis-Tris NuPAGE gels (Invitrogen, Cat. No. WG1401BOX). Samples from in vitro translation reactions of hRLuc and 3xFLAG-HBB were run on 4-12% Bis-Tris NuPAGE gels (Invitrogen ...
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bioRxiv - Developmental Biology 2021Quote: IP samples were boiled at 70°C for 10 minutes and separated on a 4-12% gradient Bis-Tris gel (Thermo Fisher Scientific, #NP0321) in 1x MOPS (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: To control efficiency of the enzymatic treatment 5 μg of the enzyme-treated products or controls were run on a NuPage™ 4-12 % Bis-Tris gel (Invitrogen, Carlsbad, USA) at 100 V for 60 minutes using the EI9001-XCELL II Mini Cell (Novex ...
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bioRxiv - Systems Biology 2022Quote: ... 20 ug of total protein from each lysate was loaded and separated on a NuPAGE 4%–12% Bis-Tris Gel (Invitrogen cat. no. NP0335BOX). Subsequently ...
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bioRxiv - Biochemistry 2022Quote: ... Extent of cleavage was determined by and SDS-PAGE analysis on 4-20% Mini-PROTEAN® TGX™ protein gels stained with GelCode™ Blue Stain Reagent (ThermoFisher). TEV digestion of CrossMAb-ACE2 fusions ...
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bioRxiv - Biochemistry 2022Quote: ... Extent of cleavage was determined by and SDS-PAGE analysis on 4-20% Mini-PROTEAN® TGX™ protein gels stained with GelCode™ Blue Stain Reagent (ThermoFisher).
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bioRxiv - Neuroscience 2021Quote: ... The concentrated RNA (10-15 µg per sample) was then size fractionated by electrophoresis using 15% polyacrylamide-urea gels (ThermoFisher Scientific, catalog # EC68852BOX) run at 200 volts in TBE (89 mM Tris-borate ...
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bioRxiv - Molecular Biology 2020Quote: Western blot analysis was carried out using standard procedures for SDS-PAGE electrophoresis on 4-12% NuPage gels in MOPS buffer (Invitrogen, Carlsbad, CA, USA), then transferred to nitrocellulose membranes (Whatman ...
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bioRxiv - Molecular Biology 2020Quote: ... placing the plate on - 20°C cold gel pack and treated with 0.4 mL/well TRIzol® LS reagent (#10296028,Ambion™, Life Technologies, USA). The TRIzol treated cell lysates from each well were harvested and stored at –80°C until use ...
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bioRxiv - Genetics 2020Quote: Proteins eluted from the beads were separated by SDS/PAGE electrophoresis on a 10% polyacrylamide gel and stained with Colloidal blue silver (ThermoFisher Scientific, USA; 70). SDS-PAGE gel lanes were cut into 2-mm slices and subjected to in-gel reduction with dithiothreitol ...
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bioRxiv - Neuroscience 2019Quote: ... Total protein was transferred to a 0.45 μm pore nitrocellulose membrane at 20 V for 7 minutes using iBlot Gel Transfer Nitrocellulose Stacks (NR13046-01, Invitrogen, Waltham, MA, USA) and an iBlot machine (Invitrogen ...
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bioRxiv - Developmental Biology 2020Quote: ... Purity of eluted gRNA product was determined by formalin gel electrophoresis and gRNA was quantified on a Nanodrop 2000c (ThermoFisher Scientific, Waltham, MA). Following quantification ...
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bioRxiv - Molecular Biology 2019Quote: ... Amplified fragments were purified using a Wizard SV Gel and PCR Clean-Up System and subcloned into pCRII using a TOPO TA Cloning™ Kit Dual Promoter (Thermo Fisher, #450640). The following primers were used.
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bioRxiv - Plant Biology 2019Quote: ... The protein solution was adjusted to equal concentration and separated on a NuPAGE™ 4-12% Bis-Tris Protein Gel (Thermo Fisher Scientific). Each sample was loaded twice on the gel allowing separation of the gel in two parts with identical samples ...
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bioRxiv - Cancer Biology 2020Quote: ... 13.7-20 µg of total protein per sample was resolved under reducing conditions on Novex NuPAGE gels (4-12% bis-tris protein gels) using LDS sample buffer and MES running buffer according to the manufacturers protocol (Life Technologies, Carlsbad, USA). Gels were stained using the Silver Staining Plus Kit and documented on the ChemiDoc XRS+ system (Bio-Rad Laboratories Inc. ...
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bioRxiv - Genetics 2019Quote: ... SDS-Page separation was completed by running 2 μg of total protein on a NuPAGE™ 3-8% Tris-Acetate gel (Thermo Fisher Scientific). Next ...
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bioRxiv - Microbiology 2021Quote: ... incubated for 5 min at 95 °C and 30 μg of protein loaded onto Bolt™ 4-12% Bis-Tris Plus precast gels (Thermo Fisher Scientific). Electrophoresis was performed at 180 V constant in 2-morpholin-4-ylethanesulfonic acid (MES)-SDS running buffer (50 mM MES ...