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Citations for Thermo Fisher :
9251 - 9300 of 9427 citations for Vertical Gel Casting Cassettes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... 20 µg of the protein samples were then heat-denatured and subsequently separated on a 4-12% Bis-Tris gel (Invitrogen; Thermo Fisher Scientific). Separated proteins were then transferred to a 0.45 µm nitrocellulose membrane via wet blotting for 3h at 300 mA ...
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bioRxiv - Neuroscience 2023Quote: ... 11 μL of each sample (see Table 1 for concentrations) was loaded onto 4–12% Bis-Tris NuPAGE polyacrylamide gradient gels (Life Technologies; Bedford, MA) for electrophoresis ...
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bioRxiv - Microbiology 2023Quote: ... 5 μL of the IP fraction for α-FLAG immunoblots were loaded on a 4-20% Novex WedgeWell SDS-PAGE gels (Thermo Fisher Scientific). For western blot analysis related to RpoD ...
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bioRxiv - Cancer Biology 2023Quote: ... Equally loaded lysates were resolved by SDS-PAGE using NuPAGE 4-12% Bis-Tris Gel (NP0321BOX, Invitrogen/Thermo Fisher Scientific, Waltham, MA, USA) and transferred to Immobilon-P Transfer Membrane (IPVH00005 ...
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bioRxiv - Cell Biology 2023Quote: ... A total of 15 µg of total protein fraction for each sample was separated by electrophoresis using NuPAGE™ 4-12% Bis-Tris Protein Gel (Thermo Fisher Scientific). Proteins were transferred to a polyvinylidene difluoride membrane using an XCell SureLock Mini-Cell Electrophoresis System (Thermo Fisher Scientific) ...
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bioRxiv - Biophysics 2023Quote: ... Purity of produced viral vectors was routinely checked by silver staining (Pierce, Germany) after gel electrophoresis (Novex™ 4–12% Tris–Glycine, Thermo Fisher Scientific) according to the manufacturer’s instruction ...
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bioRxiv - Biochemistry 2023Quote: ... and ultrapure water (to a final volume of 10 µL) to a 10 or 15 well NativePAGE™ Novex® 4 – 16% gradient Bis-Tris Gel (Life Technologies). Gels were placed in a XCell SureLock™ Mini-Cell Electrophoresis System in the cold room (∼7°C) ...
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bioRxiv - Genetics 2024Quote: ... A portion of labelled and unlabeled lipoprotein samples were analyzed by SDS-PAGE on NuPAGE 3-8% gradient Tris-Acetate gels (Thermo Fisher Scientific, # EA0375) under reducing and nonreducing conditions followed by SilverQuest Silver Staining (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... Western blots were run using 20ug of total protein per well on Bolt 4-12% Bis-Tris Plus gels (ThermoFisher Catalog Number: NW04120BOX). Gels were then transferred to Immobilon-P PVDF membrane (Millipore Catalog Number ...
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bioRxiv - Biophysics 2024Quote: ... protein fractions were mixed with SDS-free loading buffer in a 3:1 ratio and loaded on a Novex™ 4–20% Tris-Glycine Gels using NativeMark™ Unstained Protein Standards (Invitrogen™) (range from 20 to 1236 kDa ...
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bioRxiv - Immunology 2024Quote: ... Myotis myotis) 67 or Bacillus anthracis were loaded to 1-well 4-12% Bis-Tris NuPAGE Novex SDS-PAGE gels (Invitrogen, Thermo Fisher Scientific). Proteins were separated for 45 min at 200 V ...
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bioRxiv - Biochemistry 2024Quote: ... The samples were boiled at 95 °C for 10 min and run on a NuPAGE 4-12% Bis-Tris polyacrylamide gel (Thermo Fisher Cat#NP0322BOX). Bands were visualized by staining the gel with Pierce GelCode blue stain reagent following manufacturer’s protocol.
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bioRxiv - Microbiology 2024Quote: ... proteins were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoreses (SDS-PAGE) and stained using GelCode™ Blue Safe Protein Stain (Thermo Fisher Scientific). The specific activities of diaphorase and NTR were typically in the range of 100–150 and 3–5 μmol/min/mg ...
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bioRxiv - Neuroscience 2024Quote: ... Whole proteins were electrophoresed in a 10% SDS-PAGE gel and then transferred to polyvinylidene fluoride (PVDF) membranes (0.45 μm) using iBlot 2 Dry Blotting System (Thermo Fisher Scientific Systems, USA). After blocking in 5% skim milk ...
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bioRxiv - Molecular Biology 2024Quote: ... the digested vector was treated with phosphatase for a brief 10-minute duration followed by gel purification with a 2% low melting temperature agarose (ThermoFisher Cat No. 16500100) in 1X TAE buffer.
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bioRxiv - Molecular Biology 2024Quote: ... PCR products were visualized on a 2% agarose gel to ensure robust amplification and treated with ExoSAP-IT (Affymetrix, Santa Clara, CA, US) using 1 µL of ExoSAP-IT and 7 µL of PCR product under the following conditions ...
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bioRxiv - Biophysics 2024Quote: ... using NuPAGE™ 4-12% Bis-Tris Protein Gels (Thermo Fischer Scientific, NP0336BOX) in Invitrogen Novex NuPage MES SDS Running Buffer (1X, Thermo Fisher Scientific, NP0002). Primary antibody ...
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bioRxiv - Cancer Biology 2021Quote: Samples were boiled for 5 min prior to loading 7 µg of samples on to 4-15% SDS-PAGE gels alongside a molecular weight ladder (Thermo Fisher Scientific, cat# LC5602) and electrophoresis at 200V for 40 min ...
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bioRxiv - Immunology 2021Quote: ... IgG was isolated from healthy control or AAV patient serum using a Melon™ Gel IgG Spin Purification Kit (Thermo Fisher Scientific, Waltham, MA).
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bioRxiv - Immunology 2021Quote: ... Antibody purity and proper size was verified by Coomassie stained sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) (Thermo Fisher Scientific, Waltham, MA, USA).
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bioRxiv - Microbiology 2021Quote: ... Progression and quality of the chromatographies were assessed by SDS-PAGE silver-stained gels using Pierce™ Silver staining kit (ThermoFisher Scientific Cat# 24612) and standard 15% resolving gel 30:1 Polyacrylamide ...
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bioRxiv - Developmental Biology 2021Quote: ... were loaded on a Bolt™ 12% Bis-Tris Plus Gel (Life Biosciences, cat. no. NW00122BOX) and run at constant 180 V in a Bolt™ MES SDS running buffer (Invitrogen, cat. no. B0002). The proteins were transferred onto a nitrocellulose membrane in transfer buffer (25 mM Tris-HCl pH 8 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Quality and quantity of recovered high molecular weight DNA was assessed by electrophoresis on agarose gel, by spectrophotometry (Nanodrop, Saint-Remy-lés-Chevreuse, France) and with the QuBit fluorometric quantitation system (Life Technologies, Villebon-sur-Yvette, France).
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bioRxiv - Evolutionary Biology 2021Quote: ... The amplicons were separated using the agarose gel-electrophoresis and cloned using TOPO TA Cloning Kit for Sequencing (Thermo Fisher Scientific K.K., Tokyo, Japan) following the manufacture’s protocol ...
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bioRxiv - Neuroscience 2020Quote: ... One μg of total protein per sample was resolved by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) using 4-12% bis-tris gels in MOPS SDS running buffer (NuPAGE® Novex® SDS-PAGE system; Thermo Fisher Scientific) and transferred to polyvinylidene fluoride membranes (iBlot® 2 gel transfer system ...
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bioRxiv - Genomics 2020Quote: ... The DNA quality and concentration were assessed using electrophoresis on 1.0% agarose gels and an ND-2000 spectrophotometer (Thermo Fisher Scientific, Wilmington, DE, USA). The genomic DNA was used for Illumina and ONT library construction and then sequencing ...
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bioRxiv - Biochemistry 2022Quote: ... final expression clones were validated by agarose gel electrophoresis and restriction digest and were used to generate bacmid DNA with the Bac-to-Bac system (Thermo Fisher Scientific, Waltham, MA) using the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2020Quote: ... placing the plate on - 20°C cold gel pack and treated with 0.4 mL/well TRIzol® LS reagent (#10296028,Ambion™, Life Technologies, USA). The TRIzol treated cell lysates from each well were harvested and stored at –80°C until use ...
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bioRxiv - Genomics 2020Quote: ... the PCR products were separated by agarose gel electrophoresis and sequenced from both ends on the Applied Biosystems 3500 XL DNA Analyzer platform (ThermoFisher Scientific, Waltham, MA, USA) following the manufacturer’s guideline.
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bioRxiv - Genomics 2020Quote: ... DNA and RNA quality were evaluated by agarose gel electrophoresis and their quantities determined using a NanoDrop spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA).
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bioRxiv - Cancer Biology 2019Quote: ... IP products or 60 µg input lysates were resolved on 4–12% Tris-Glycine SDS-PAGE gels and immunoblotted with an anti-V5 antibody (Life Technologies R96205, 1:5,000), Bethyl anti-PDE3A antibody (1:1,000) ...
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bioRxiv - Microbiology 2019Quote: ... Samples were mixed with an equal volume of NuPAGE® Tris-glycine native sample buffer (Novex®) and loaded on to NuPAGE™ 3-8% Tris-acetate gel (Thermofisher, USA). Gel blots were run at 150 V for 3 hours ...
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bioRxiv - Genomics 2020Quote: ... DNA quality was assessed by electrophoresis on 3% agarose gels and DNA concentration was determined by dsDNA HS Qubit Assay (Molecular Probes by Life Technologies, No. Q32851). The Center for Genome Research and Biocomputing at Oregon State University generated 94 Wafergen DNA 150bp paired-end libraries using the Biosystems Apollo 324 NGS library prep system ...
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bioRxiv - Biochemistry 2020Quote: Electrophoresis was performed under reducing and denaturing conditions using Invitrogen NuPAGE 12% Bis-Tris precast protein gels with 12 wells (Thermo Fisher Scientific, CA, USA). Non-fermented samples were mixed with NuPAGE™ LDS Sample Buffer (containing glycerol ...
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bioRxiv - Plant Biology 2020Quote: ... The integrity of RNA was tested on a bleach agarose gel (Aranda et al., 2012) and for purity using NanoDrop OneC (Thermo Fisher Scientific, Braunschweig, Germany).
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bioRxiv - Microbiology 2021Quote: ... The presence and size of amplicons were confirmed by gel electrophoresis using a DNA ladder to identify amplicon size (GeneRuler 1kb Plus, Thermo Fisher Scientific, Emeryville, CA). Negative controls ...
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bioRxiv - Neuroscience 2021Quote: ... NuPAGE 4 × loading buffer was added to all lysates and then boiled for 5 min before loading onto a Tris-glycine gel (Thermo Scientific, Waltham, MA, USA) for electrophoresis ...
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bioRxiv - Cancer Biology 2020Quote: One-third of the eluate was then run on a 10-20% Tris-Glycine SDS Page gel and stained with SimplyBlue Commassie stain (Thermo Fisher Scientific, Waltham, MA) for 2 hours ...
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bioRxiv - Evolutionary Biology 2020Quote: ... used as a template in cycle sequencing reactions and run on 4% denaturing polyacrylamide gels in an ABI 377 automated sequencer (Applied Biosystems, Foster City, California) using a Big-Dye Terminator Cycle Sequencing kit (Applied Biosystems ...
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bioRxiv - Microbiology 2020Quote: ... The library fragment sizes were measured using agarose gel electrophoresis and the concentrations using Qubit dsDNA HS Assay Kit (Life Technologies, Carlsbad, California, USA) and NEBNext Library Quant Kit for Illumina (New England BioLabs ...
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bioRxiv - Microbiology 2022Quote: ... A target-sized DNA of the purified library (approximately 380–510 base pairs [bp]) was then excised using E-Gel SizeSelect (ThermoFisher Scientific, Waltham, MA, USA). Sequencing was performed using MiSeq 250 paired-end sequencing at Ryukoku University ...
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bioRxiv - Molecular Biology 2022Quote: ... PCR success was evaluated on a 1 % agarose gel before PCR products were normalised using a SequalPrep Normalisation plate (Thermo Fisher Scientific, MA, USA) following the manufacturer’s instructions with an end concentration of 25 ng per sample (100 μl) ...
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bioRxiv - Molecular Biology 2022Quote: ... 20 µg of protein was mixed with NuPAGE sample reducing agent and NuPAGE LDS sample buffer and run on NuPAGE 3 to 8% Tris-Acetate gel (all Thermo Fisher Scientific, Abingdon, UK). Protein was electrotransferred onto a 0.45 µm polyvinylidene difluoride (PVDF ...
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bioRxiv - Microbiology 2022Quote: ... Quality of mRNA reporters were ensured by sequencing PCR products used for in vitro transcription and running final RNA products on an agarose gel using NorthernMax™-Gly Sample Loading Dye (Thermo Fisher Scientific AM8551) and Invitrogen™ Ambion Millennium Markers RNA Markers Formamide (Thermo Fisher Scientific AM7151).
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bioRxiv - Biophysics 2022Quote: ... and 1 μL α-32P-GTP (Perkin-Elmer) were incubated at 30°C for 10 minutes prior to addition of a 2x stop solution (Invitrogen-Gel Loading buffer II). Assay buffer was 100 mM K-acetate ...
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bioRxiv - Plant Biology 2022Quote: ... pastoris-produced LysM protein solution was tested using a protein polyacrylamide gel followed by CBB and glycoprotein staining with the Pierce Glycoprotein Staining Kit (Thermo Fisher Scientific, California, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... The resulting gels were washed 3 times in distilled deionized H2O for 15 minutes each and visualized by staining overnight with EZ-Run Protein Gel Staining Solution (Thermo Fisher Scientific, Cat# BP36201). Stained protein gel regions were typically excised into 4 gel sections per gel lane ...
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bioRxiv - Cancer Biology 2022Quote: ... and samples were heated at 95°C for 5mins to denature protein and later loaded onto a 4-12% Bis-Tris gel (Thermo Fisher Scientific, NuPAGE, NP0322BOX). Proteins from the gel were transferred to 0.2 μm nitrocellulose membrane (Bio- Rad ...
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bioRxiv - Immunology 2020Quote: ... The cell lysates containing approximately 20 µg of protein were resolved on Novex™ NuPAGE™ 4-12% Bis-Tris Protein Gels (Invitrogen, Waltham, MA). The separated proteins were transferred to nitrocellulose membranes that were subse-quently blocked using 5% non-fat dry milk diluted in TBST (10 mM Tris-HCL ...
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bioRxiv - Cell Biology 2019Quote: ... Matrigel was covalently attached to the polyacrylamide gels and to amino-silane coated glass with the bifunctional cross-linker Sulfo-SANPAH (Thermo Fisher Scientific, Waltham, USA), respectively ...