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Citations for Thermo Fisher :
9101 - 9150 of 9427 citations for Vertical Gel Casting Cassettes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... Beads were immediately placed immediately onto a magnetic rack and the entire sample was immediately loaded into 10% Bis-Tris polyacrylamide gels (Invitrogen, ThermoFisher catalog #NP0303BOX) and transferred to PVDF membranes (Immobilon-P ...
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bioRxiv - Bioengineering 2021Quote: ... biocompatibility of the different gel formulations or tissue construct types was assessed using a standard LIVE/DEAD cell viability/cytotoxicity kit (Thermo Fisher, Waltham, MA) according to the manufacturer’s recommendation ...
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bioRxiv - Bioengineering 2020Quote: Nanobody (2 mg) was incubated at room temperature overnight in Pierce™ immobilized TCEP disulfide reducing gel (7.5 v/v %) (ThermoFisher Scientific, MA, USA) to selectively reduce C-terminal cysteine following a previously established protocol 31 ...
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bioRxiv - Biochemistry 2020Quote: ... Samples were run on 6% TBE gel in 1 x TBE (150 V, 90 min, 4°C) stained with SYBR Gold (Thermo Fisher Scientific. S11494) and InstantBlue Protein Stain (Expedeon).
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bioRxiv - Cell Biology 2021Quote: ... or an in-house quality control (QC) protein sample was added per well of a 12% polyacrylamide gel along with 8 μl PageRuler Standard (Thermo Fisher, Cat #26616). Samples were prepared such that 2 identical blots were run at the same time ...
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bioRxiv - Cell Biology 2021Quote: ... At the end with SYBR green (solution 1:10000 in PBS 1X, SYBRTM green I Nucleic Acid Gel Stain, INVITROGEN, Eugene, Oregon, USA) DNA was stained ...
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bioRxiv - Neuroscience 2020Quote: ... Separation of the sequences was performed on the ABI 3730xl DNA Analyzer (injection time: 5 sec, run duration: 40 min, separation gel: POP7, Applied Biosystems, Waltham, USA).
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bioRxiv - Biochemistry 2021Quote: Protein samples were heated for 5 min at 95 °C and separated by electrophoresis in NuPAGE™ 4-12 % gels (ThermoFisher, Cat# NP0329BOX). RNA samples were heated for 2.5 min at 95 °C and separated by electrophoresis in denaturing 9 % acrylamide (19:1 ...
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GFI1 cooperates with IKAROS/IKZF1 to activate gene expression in T-cell acute lymphoblastic leukemiabioRxiv - Biochemistry 2021Quote: ... Whole lanes of SDS-PAGE gel were cut out and subjected to LTQ Orbitrap Velos Pro ion-trap mass spectrometry (ThermoFisher Scientific, Waltham, MA). Peptides were detected ...
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bioRxiv - Cell Biology 2021Quote: ... Depletion of the targeted DNA region was confirmed by isolating the amplified DNA of the PCR product using the PureLink Quick Gel Extraction Kit (Thermo Fisher Scientific, K210012) and Sanger sequencing (Macrogen ...
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bioRxiv - Microbiology 2021Quote: ... was electrophoresed on a NuPAGE™ 4 to 12% gel and then transferred to a nitrocellulose membrane by the iBlot2 system (Thermo Fisher Scientific) then blocked in 5% nonfat dry milk-TBST for 1 h ...
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bioRxiv - Microbiology 2021Quote: ... The gel was stained for 30 min in 0.5× TBE buffer containing 1× SYBR Gold nucleic acid staining solution (Life Technologies, Grand Island, NY), and then ...
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bioRxiv - Microbiology 2021Quote: Resulting gel lanes containing a mixture of fungal and mammalian proteins were excised and fractioned into five sections prior to in-gel protein digestion and were analyzed on an Orbitrap Fusion™ Tribrid™ (Thermo Scientific) mass spectrometer at the Herbert Irving Comprehensive Cancer Center (Columbia University Medical Center ...
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bioRxiv - Microbiology 2021Quote: BN-PAGE was performed according to the user manual of the Native PAGE Novex Bis-Tris Gel System (Thermo Fisher Scientific, Waltham, MA). For BN-PAGE of sodium cholate treated Dimer ...
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bioRxiv - Developmental Biology 2021Quote: ... then denatured at 70°C for 10 minutes and loaded into 12-well NuPAGE Novex 4-12% Bis-Tris precast gels (Thermo Fisher Scientific – NP0322BOX). The pre-stained Novex Sharp protein standard (Invitrogen – LC5800 ...
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bioRxiv - Molecular Biology 2020Quote: ... Fifty micrograms of total denatured protein were then separated by SDS-PAGE using precast NuPAGE 4-12% Bis-Tris protein gels (Invitrogen, Waltham, MA, USA) followed by electrotransfer onto polyvinylidene difluoride (PVDF ...
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bioRxiv - Microbiology 2020Quote: ... The PCR products were run on 1% agarose gels (1Tris-acetate EDTA [TAE] buffer) and analyzed using molecular imaging software (Thermo Fisher Scientific, Poland).
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bioRxiv - Microbiology 2020Quote: ... DNA quantity and quality were assessed in 1% agarose gel and using a Nanodrop 2000c spectrophotometer (Thermo Fisher Scientific Inc., Wilmington, DE, USA) set for determining absorbance at the following wavelengths ...
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bioRxiv - Cell Biology 2021Quote: Samples were run in NuPAGE Bis-Tris gels (4%-12% [Invitrogen NP0321] for small volumes of samples, 10% [Invitrogen NP0315] for larger amounts) in 1x MOPS SDS running buffer (Invitrogen NP0001 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Proteins were separated by SDS-PAGE using hand cast gradient gels (4-12%) and transferred to nitrocellulose membrane (20V for 10mins) using the iBlot® blotting system (Thermo Scientific, UK). Membranes were blocked with 5% (w/v ...
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bioRxiv - Biophysics 2020Quote: ... 20 μL of protein solution was incubated with 4 μL of LDS sample buffer and incubated at 100°C for 5 mins before 10 μL was loaded on a 4-12% bis-tris gel (NuPAGE™, Thermo Fisher Scientific). The gel was either stained with Coomassie blue or transferred to a 0.22 μm polyvinylidene fluoride (PVDF ...
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bioRxiv - Cancer Biology 2021Quote: ... the input DNA quality was verified using gel electrophoresis and the quantity was measured using the Picogreen assay (Thermo Scientific, Waltham, MA, USA). 2ng of DNA was prepared in 30ul of buffer and used for library construction using the Nextera DNA Flex Library Kit (Illumina ...
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bioRxiv - Genomics 2021Quote: ... and the quantity of Tn5 was measured by Bradford assay and visualized on NuPAGE Novex 4-12% Bis-Tris gel (Thermo Fisher Scientific, NP0321) followed by Coomassie blue staining.
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bioRxiv - Cell Biology 2020Quote: ... were incubated at 70°C for 10 min and separated on a Novex™ NuPAGE™ 4-12 % Bis-Tris Mini Protein Gel (Art. No. NP0321, Invitrogen™) in 1x Novex™ NuPAGE™ MOPS SDS Running Buffer (Art ...
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bioRxiv - Physiology 2022Quote: ... Both the digested PCR product and the linearized pUltra-hot vector were gel-purified and ligated with T4 DNA ligase (Thermo Fisher Scientific, EL0011) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were separated on a 10% SDS-PAGE gel with equivalent amounts of protein loaded in each lane (Thermo-Fisher Scientific, Waltham, MA). After transfer to nitrocellulose ...
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bioRxiv - Cell Biology 2022Quote: ... 10 μL of sample (which is the equivalent of 10 embryos) was loaded into each lane of a 3-8% Tris-Acetate pre-cast SDS-PAGE gel (Invitrogen, Thermo Fisher Scientific) and afterwards transferred from the gel onto a nitrocellulose membrane (BIO-RAD ...
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bioRxiv - Biochemistry 2022Quote: Western blot: Pancreatic and islet lysates were run on a 1 mm 4-12% Bis-Tris gel (Novex Life Technologies; Thermo Fisher; #NP0321) with MES SDS running buffer (Invitrogen ...
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bioRxiv - Biochemistry 2022Quote: Western blot: Pancreatic and islet lysates were run on a 1 mm 4-12% Bis-Tris gel (Novex Life Technologies; Thermo Fisher; #NP0321) with MES SDS running buffer (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: ... 20-50 μg of protein lysate of each sample was loaded and separated on 4-12% Bis-Tris gels (Thermo Fisher or GenScript) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... and transferred using electrophoresis to nitrocellulose membranes by using the iBlot 2 Gel Transfer Device (Thermo Scientific, iBlot 2 NC Regular Stacks, IB23001, Invitrogen, Walham, MA). Membranes were blocked for 1 h at RT in 5% (w/v ...
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bioRxiv - Biophysics 2022Quote: 50 µg purified virion sample were heated to 100 °C for 15 min and characterized by 4 to 12% NuPAGE™ Bis-Tris gel (Invitrogen, Carlsbad, CA). The protein band was stained by One-Step Blue Protein Gel Stain (Biotium ...
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bioRxiv - Molecular Biology 2022Quote: ... The DNA quality was evaluated with electrophoresis in a 1% (w/v) agarose gel and DNA concentrations were evaluated using Qubit (Thermo Fisher Scientific, USA). An equal amount of DNA from each sample was used for library preparation with the NEBNext DNA Ultra2 Library Preparation Kit (New England Biolabs) ...
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bioRxiv - Cell Biology 2022Quote: ... The samples were heated at 70 °C for 10 minutes before they were loaded on a NuPAGE™ 12% gradient Bis-Tris Protein Gel (ThermoFisher Scientific, UK) and allowed to run at a constant current of 125 V ...
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bioRxiv - Microbiology 2020Quote: ... and prokaryotic cells were stained with SYBR® Green I Nucleic Acid Gel Stain (9x in final concentration, Thermo Fisher Scientific, Darmstadt, Germany) following a protocol of Giebel ...
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bioRxiv - Plant Biology 2021Quote: ... DNA quality was checked by agarose gel electrophoresis and quantified with a NanoDrop spectrophotometer ND-1000 version (Thermo Fisher Scientific, Wilmington, DE, USA). For GBS library construction ...
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bioRxiv - Plant Biology 2021Quote: ... The PCR reaction was then run on a 1% agarose TAE gel with a 1 kb plus DNA ladder (Invitrogen; Carlsbad, CA, USA), excised and gel extracted using the Monarch DNA Gel Extraction Kit following the manufacturers’ instructions (New England BioLabs ...
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bioRxiv - Plant Biology 2021Quote: ... The PCR reaction was then run on a 1% agarose TAE gel with a 1 kb plus DNA ladder (Invitrogen; Carlsbad, CA, USA). All primers are listed in Supplemental Data Set 2.
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bioRxiv - Microbiology 2020Quote: ... The PCR products were run on 1% agarose gels (1Tris-acetate EDTA [TAE] buffer) and analyzed using molecular imaging software (Thermo Fisher Scientific, Poland).
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bioRxiv - Cell Biology 2021Quote: ... 15μg of protein were ran on a Novex™ Bolt 4-12% bis-tris mini gel with NuPAGE™ 1x MES SDS running buffer (ThermoFisher Scientific). 5μl of PageRuler™ Plus Prestained Protein Ladder (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... DNA concentration and purity were determined by agarose gel electrophoresis and fluorometrically using RiboGreen (Qubit Assay Kit, Invitrogen, Life Technologies Corporation, Oregon, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: All samples were heated for 5 min at 100°C and loaded onto a precast NuPAGE 12% Bis-Tris Gel (Life Technologies; Carlsbad, CA). The gels were electrophoresed at 180V for 60 min and proteins were transferred to PVDF membranes (Life Technologies ...
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bioRxiv - Immunology 2022Quote: ... The quality and concentration of DNA were determined by 1.0% agarose gel electrophoresis and a Nano Drop® ND-2000 spectrophotometer (Thermo Scientific Inc., USA) and kept at −80 ℃ prior to further use ...
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bioRxiv - Cell Biology 2022Quote: ... The polymerized collagen gel was then labeled with 50 μg.ml-1 Alexa Fluor 594-conjugated-NHS ester dye (A20004, Thermo Fisher, Waltham, MA, USA) in pH = 8.7 NaHCO3 buffer for 1 h at 37 °C ...
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bioRxiv - Plant Biology 2019Quote: ... All PCR-amplified fragments were analyzed by taking 10 μL of reaction and run on an electrophoresis gel of 1.5% agarose (Life Technologies, Carlsbad, CA, USA) and 1x TBE (50 mM Tris-HCl pH 8 ...
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bioRxiv - Molecular Biology 2019Quote: ... RNA samples were heated at 80°C for 2 min and 40-50 mg of RNA were loaded onto 15% TBE-Urea polyacrylamide gels (Invitrogen; Waltham, MA, USA) in 1x TBE (Ambion ...
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bioRxiv - Molecular Biology 2020Quote: ... The reaction was incubated 2h at 37°C and then loaded on a 15% acrylamide/8M urea precast gel (Invitrogen, cat n° EC6885BOX). The ligated RNA was purified through gel extraction ...
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bioRxiv - Neuroscience 2020Quote: ... Total protein lysates (15-50 µg) were subjected to SDS-PAGE using NuPAGE 4-12% Bis-Tris Midi Protein Gels (Invitrogen, Carlbad, MA, USA) and subsequently transferred to Polyvinylidene difluoride (PVDF ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and visually evaluated by gel electrophoresis on a 1.2% agarose gel (GelRed nucleic acid stain, Lambda DNA/HindIII Marker and 1kb DNA marker (Invitrogen; Thermo Fisher Scientific, Germany)).
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bioRxiv - Pathology 2021Quote: ... The DIG-labeled TiLV probes for each segment was obtained after purifying amplified product by NucleoSpin™ Gel PCR Clean-up Kit (Fisher Scientific, US) according to the manufacturer’s protocol ...