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Citations for Thermo Fisher :
8901 - 8950 of 9427 citations for Vertical Gel Casting Cassettes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... Virion samples were heated 72°C for 10 minutes and 15 μl virion lysate was loaded into each well of a NuPAGE™ 4 to 12% Bis-Tris gels (ThermoFisher NP0329BOX). For cell lysates ...
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bioRxiv - Microbiology 2022Quote: ... The RNA samples were then separated by 12% Urea-PAGE followed by gel extraction and subsequent reverse transcription using Superscript III reverse transcriptase (Invitrogen #18080–044). The cDNA was PCR amplified and sequenced on Illumina Hi-Seq 4000 ...
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bioRxiv - Microbiology 2022Quote: ... and wet transfer (Tris-Glycine-20% Methanol) on 0.2 μm Nitrocellulose membrane (Amersham™ Protran™) were performed using the Mini Gel Tank (Life technologies). Membranes were subsequently subject to blocking in 5% (v/v ...
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bioRxiv - Microbiology 2022Quote: ... the integrity of the RNA was evaluated on a 1% agarose gel and RNA concentration was determined by NanDrop2000 (Thermo Fisher Scientific). To verify the absence of residual gDNA we performed a PCR on the RNA samples ...
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bioRxiv - Microbiology 2022Quote: ... and 150 mM NaCl and 10 µL of 2X loading dye and then ran on a NuPAGE™ 4-12% Bis-Tris Gel (Invitrogen, #NP0335) and stained with InstantBlue® (Abcam ...
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bioRxiv - Microbiology 2022Quote: ... 4-12% or 12% NuPAGE® Bis-Tris mini gel in a XCell SureLock® Mini-Cell filled with either 1x MES or 1x MOPs SDS running buffer (Invitrogen, USA) was used and run at 140 V for 90 min ...
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bioRxiv - Neuroscience 2023Quote: ... 10 colonies of each npc1 heterozygous mutant were amplified and double checked by 1% agarose gel electrophoresis and Sanger sequencing with 3730xl DNA Analyzer (Thermo Fisher Scientific). Finally ...
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bioRxiv - Physiology 2023Quote: ... followed by separation for 45 min at 200 V in MOPS buffer in pre-cast 4 – 12% gradient Bis-Tris precast gels (Nupage, Thermo Fisher Scientific). This was followed by transferring onto nitrocellulose membranes (0.45-μm pore size ...
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bioRxiv - Synthetic Biology 2022Quote: ... in 1X TAE) were stained with biotium GelRed® Nucleic Acid Gel Stain and bands were imaged using an iBright imaging system (Thermo Fisher). The 1 kb plus DNA ladder (NEB ...
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bioRxiv - Neuroscience 2022Quote: ... from each sample were run on 4-12% SDS gels and transferred to nitrocellulose membranes with the iBlot® dry blotting system (Life Technologies) for 7 min ...
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bioRxiv - Molecular Biology 2022Quote: ... The eluted samples were heated at 95°C for 10 minutes with 1x reducing sample buffer and loaded into a 4%-20% tris-glycine gel (Thermo Fisher Scientific) at 30 µL/well ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were run in a 15% denaturing acrylamide gel and deamination activity was detected by fluorescence using iBright CL1500 imaging system (Thermo Fisher Scientific).
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bioRxiv - Molecular Biology 2022Quote: ... The gel extracted insert was then ligated to the gel extracted backbone from the RFP+TORNADO Broccoli digest in a 20 μL T7 ligation reaction (Thermo Fisher Scientific) at room temperature for 30 minutes ...
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bioRxiv - Molecular Biology 2022Quote: ... was then ligated to the gel extracted backbone from the RFP+TORNADO Broccoli digest in a separate 20 μL T7 ligation reaction (Thermo Fisher Scientific) at room temperature for 30 minutes (12 total reactions) ...
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bioRxiv - Immunology 2022Quote: ... and 3-10μg of total protein cell lysates was run on 4 to 12% NuPAGE Bis-Tris SDS-PAGE gels (Invitrogen by ThermoFisher Scientific) and transferred to polyvinylidene difluoride membranes (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... the PCR products were gel purified and analyzed by Sanger sequencing (City of Hope Integrative Genomics Core, Applied Biosystems 3730 DNA Analyzer), which was used for TIDE analysis (Brinkman et al ...
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bioRxiv - Cell Biology 2022Quote: ... were boiled for 5 min and subjected to SDS-PAGE using NuPAGE™ 4-12% Bis-Tris Gels (Invitrogen; Thermo Fisher Scientific) at 150V for 120 min and transferred to 0.45 μm PVDF membranes (Sigma ...
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bioRxiv - Cell Biology 2022Quote: ... were boiled for 5 min and subjected to SDS-PAGE using NuPAGE™ 4-12% Bis-Tris Gels (Invitrogen; Thermo Fisher Scientific) at 150V for 120 min and transferred to 0.45 μm PVDF membranes (Sigma ...
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bioRxiv - Cancer Biology 2024Quote: ... The resolved samples were run on either NuPAGE 3–8% Tris-acetate or NuPAGE 12% Bis-Tris protein gels (Thermo Fisher Scientific) with the appropriate running buffer ...
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bioRxiv - Cancer Biology 2024Quote: ... lysates were extracted with Laemelli sample buffer for 10 minutes at 75°C and lysates were run on 4% to 12% Bis-Tris gels (Invitrogen; Thermo Fisher Scientific). Proteins were transferred to nitrocellulose membranes (Prometheus ...
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bioRxiv - Molecular Biology 2024Quote: ... with GTS running buffer (Tris 25 mM, Glycine 190 mM, SDS 0.1%) or 4-12% Bis-Tris precast NuPAGE gels (Thermo Fisher Scientific NP0322BOX) with MOPS running buffer (Thermo Scientific NP0001) ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA quality and concentration were determined by MOPS-formaldehyde denaturing agarose gel electrophoresis and Qubit RNA BR Assay Kit (Thermo Fisher Scientific).
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bioRxiv - Genomics 2023Quote: ... We assessed total RNA sample quality on a 1% agarose gel and quantified the RNA in each sample with a Qubit 2.0 High Sensitivity kit (ThermoFisher Scientific, Waltham, MA). We then pooled developmental stages (embryos and juveniles ...
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bioRxiv - Cell Biology 2024Quote: We prepared polyacrylamide gels with a stiffness of 18kPa according to a previously established protocol and functionalized them with Sulpho-SANPAH (Thermo Fisher Scientific)72 ...
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bioRxiv - Biophysics 2024Quote: ... Each sample was prepared with a final concentration of 0.1 mg/mL CaMKIIα and 8x SYPRO® Orange Protein Gel Stain (Life Technologies, #S6650) in MES buffer (20 mM MES ...
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bioRxiv - Cell Biology 2024Quote: ... DNA fragments from the beads and input samples were purified using PureLink™ Quick Gel Extraction and PCR Purification combo kit (Thermo Scientific) after decrosslink ...
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bioRxiv - Cell Biology 2024Quote: ... IP samples were run on a 4-12% Bis-Tris gel and washed three times with water before staining with Gelcode Blue Stain Reagent (Thermo Fisher 24590) overnight rocking ...
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bioRxiv - Molecular Biology 2024Quote: ... boiled at 80 °C for 10 min and proteins separated using precast NuPAGE 4–12 % Bis-Tris gels (Thermo Fisher Scientific, NP0322PK2) at 80 V for ca ...
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bioRxiv - Molecular Biology 2024Quote: ... The purity of all newly synthesized RNAs was detected using UREA-polyacrylamide gel electrophoresis (PAGE) which was stained with SYBR™ Gold (Invitrogen, S11494). The gels were finally visualized on a ChemiDoc MP imaging system (Bio-Rad).
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bioRxiv - Plant Biology 2024Quote: ... PCR products were run on 1% or 2% TAE-agarose gels with 1000 bp or 50 bp GeneRuler (ThermoFisher Scientific Inc., Germany) depending on product size ...
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bioRxiv - Plant Biology 2024Quote: ... 100 ng of total proteins in ‘day 25’ were separated on a 10% (w/v) Bis-Tris gel (Invitrogen, Waltham, MA, USA) run for 1 h at 190 V in Novex Nupage (Invitrogen ...
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bioRxiv - Physiology 2024Quote: ... 10-15 μg of placental homogenates or 5-10 μg of TPM were loaded onto a NuPAGE Bis-Tris Gels (Thermo Fisher Scientific), and electrophoresis was performed using MOPS-SDS running buffer at a constant 100 V for 1 h ...
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bioRxiv - Neuroscience 2024Quote: ... and 40 μg of protein lysates were resolved on precast 3-8% gels in Tris-acetate buffer and then transferred to nitrocellulose membranes (all from Thermo Fisher Scientific). Membranes were blocked with 5% (w/v ...
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bioRxiv - Genetics 2024Quote: ... Proteins were separated in denaturing conditions using Invitrogen Novex™ WedgeWell™ 8-16% gradient Tris–glycine acrylamide gels (Thermo Fisher Scientific) and transferred using a wet electroblotting system (Bio-Rad) ...
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bioRxiv - Immunology 2024Quote: ... 0.5% Coomassie G-250 was added to each soluble fraction immediately prior to loading samples on native PAGE 4 to 16% bis-tris mini gels (Thermo Fisher Scientific). Electrophoresis was performed at 4°C for 30 min in dark blue cathode at 150 V (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... 20-50 mg of total protein were loaded per lane on a Novex™ 4-20% Tris-Glycine gradient gel (Invitrogen; XP04202BOX) and electrophoretically transferred to a nitrocellulose membrane (Bio-Rad ...
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bioRxiv - Cancer Biology 2023Quote: ... protein lysates were loaded onto a precast 4-12% gradient SDS-PAGE gel from the NuPAGE SDS-PAGE system (Invitrogen, Carlsbad, CA). The gel was run at 200 V for 40 min. ...
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bioRxiv - Microbiology 2023Quote: ... from the six macaques immunized with the highest dose of DENVLP were pooled together and purified using Melon Gel IgG Spin Purification Kit and NAb Spin column (ThermoFisher #45206, #89961) followed by buffer exchange to PBS via Slide-A-Lyzer dialysis cassettes (ThermoFisher #66012 ...
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bioRxiv - Genetics 2023Quote: A pooled equimolar mixture of the ChIP-ISO synthetic oligonucleotide library was PCR-amplified and purified via agarose gel purification (Thermo Scientific K0691). The EMSA protocol was adapted from Garcia et al ...
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bioRxiv - Cell Biology 2023Quote: ... were boiled for 5 min and subjected to SDS-PAGE using NuPAGE™ 4-12% Bis-Tris Gels (Invitrogen; Thermo Fisher Scientific) at 120V for 80 min and transferred to 0.45 µm PVDF membranes (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... see the “PAA elastic gel premix” section) of the projected rigidity G’ = 50 kPa(75) with the addition of the 5% streptavidin-acrylamide (ThermoFisher, Cat# S21379) were ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were run on 4-12% Bis-Tris polyacrylamide gels (Novex, NP0322) in NuPage® SDS MOPS running buffer (ThermoFisher Scientific, NP0001). Proteins were then electro-transferred onto polyvinylidene fluoride (PVDF ...
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bioRxiv - Developmental Biology 2023Quote: ... and equal amounts of proteins (∼20 µg) were resolved on NuPAGE 4–12% Bis-Tris gels (Novex® by Invitrogen™ - WG1401BX10) under reducing conditions ...
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bioRxiv - Cell Biology 2023Quote: ... were boiled for 5 min and subjected to SDS-PAGE using NuPAGE™ 4-12% Bis-Tris Gels (Invitrogen; Thermo Fisher Scientific) at 120V for 80 min and transferred to 0.45 µm PVDF membranes (Sigma ...
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bioRxiv - Biochemistry 2023Quote: ... The quenched samples were analyzed by SDS-PAGE (12% Bis-Tris gel in SDS running buffer) and stained with SYPRO Ruby (ThermoFisher Scientific: #S12000) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: Detergent-solubilized brain and dorsal root ganglion (DRG) tissue lysates were electrophoresed through 4% to 20% precast polyacrylamide gradient gel (Invitrogen, Waltham, MA), transferred to activated PVDF (Immobilon-P ...
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bioRxiv - Immunology 2023Quote: ... Isolated bovine CD14+ Mo and boPBMCs excluding CD14+ Mo were solubilised in Laemmli sample buffer with or without DTT and separated on 12 % Bis-Tris Gels (Thermo Fisher Scientific) using MES (immunogen ...
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bioRxiv - Microbiology 2023Quote: ... The protein was separated by a 5–20% polyacrylamide gradient gel (ATTO, Tokyo, Japan) and transferred to a polyvinylidene difluoride (PVDF) membrane using iBlot 2 Dry Blotting System (Thermo Fisher Scientific). The membrane was then incubated in 5% skim milk (FUJIFILM Wako ...
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bioRxiv - Microbiology 2022Quote: ... and 3 μg of cell lysate or ∼20 μL of supernatant was separated on a precast 12% or 4-12% Bis-Tris polyacrylamide gel before being transferred to a polyvinylidene fluoride (PVDF) membrane using an iBlot2 dry blotting system (Thermo Fisher Scientific). The blot was then probed with anti-SARS-CoV-2-spike RBD (GTX135385 ...
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bioRxiv - Microbiology 2023Quote: ... Blood was collected by terminal cardiac puncture immediately after CO2 asphyxiation and transferred to serum gel blood tubes (Thermo Fisher Scientific, MA). After a minimum of 20 minutes at room temperature ...