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Citations for Thermo Fisher :
8951 - 9000 of 9427 citations for Vertical Gel Casting Cassettes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: Equal amounts of lysate were denatured for 10 minutes at 80°C and loaded on a 4 to 12% polyacrylamide Bis-Tris gel (Thermo Scientific #NW04120BOX). The gel was run at 120V for approximately 80 minutes in MOPS buffer ...
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bioRxiv - Genomics 2023Quote: ... DNA fragments between 125-300 bp (size extendable up to 700 bp) were recovered by 2% E-gel (Invitrogen, cat. no. G402002) and purified using Zymo Gel DNA recovery kit (Zymo Research ...
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bioRxiv - Genomics 2023Quote: ... The DNA integrity of the pool was controlled on 1% agarose gel and quantified on Nanodrop and Qubit using the Qubit™ dsDNA HS Assay Kit (Invitrogen™).
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bioRxiv - Cell Biology 2023Quote: ... Each gel was then transferred to a 0.45 µm PVDF membrane using 10% methanol-supplemented 1X NuPAGE Transfer Buffer (Thermo Fisher Scientific, #NP0006) for 1 hour at 30 volts ...
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bioRxiv - Immunology 2023Quote: ... 63 mM Tris-HCl pH 6.8) and ran on a pre-cast Novex Tris-Glycine Mini Protein gel (Thermo Fisher Scientific, XP04125BOX) at 70 V for 12 min ...
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bioRxiv - Cell Biology 2023Quote: ... Protein samples were subjected to SDS-PAGE on a 10% polyacrylamide gel followed by Western blotting using an iBlot 2 Dry Blotting System (Thermo Fisher Scientific). Rabbit polyclonal anti-β-catenin antibody ...
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bioRxiv - Neuroscience 2023Quote: Samples (input (eluted in 2× SDS buffer and thawed 15 minutes at 75°C) and IP proteins) were run in NuPAGE 4%–12% Bis-Tris Mini Protein Gel (10 wells) (ThermoFisher Scientific, NP0321BOX) at the voltage of 100V for 2.5 hours in a NuPAGE MOPS SDS Running buffer (ThermoFisher ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were then loaded into NuPAGE Novex 4-12 % Bis-Tris Protein Gels in MOPs or MES SDS running buffer (both Thermo Fisher Scientific) and SDS-PAGE was conducted by the application of 200 V for 50 minutes or 120 V for 1.5 hours ...
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bioRxiv - Neuroscience 2023Quote: ... SDS-PAGE was conducted by running samples on NuPAGE Novex 4-12 % Bis-Tris Protein Gels in MOPs or MES SDS running buffer (both Thermo Fisher Scientific) at 120 V for 1.5 hours ...
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bioRxiv - Genomics 2023Quote: ... DNA concentration and quality were examined by electrophoresis on a 2% (w/v) agarose gel and a Qubit 3.0 fluorometer (Thermo Scientific, Wilmington, USA), respectively.
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bioRxiv - Molecular Biology 2023Quote: ... samples were purified and digested using a 12% separating gel before being loaded onto the Ultimate 3000 nano UHPLC system (ThermoFisher Scientific, USA). 1 μg of samples were loaded (mobile phase ...
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bioRxiv - Microbiology 2023Quote: ... aeruginosa and Salmonella organisms were prepared and separated by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE) as described (18) with a Novex X-Cell Surelock minicell system (Invitrogen, Carlsbad, CA). Tris-bis-polyacrylamide gels (12.5% ...
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bioRxiv - Microbiology 2023Quote: ... Purity and integrity of all proteins were checked and protein concentrations estimated using quantitative densitometry on GelCode Blue-stained protein gels (Thermo Fisher Scientific) containing bovine serum albumin (BSA ...
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bioRxiv - Molecular Biology 2023Quote: ... proteins were transferred to a nitrocellulose membrane (iBlot 2 Transfer Stacks, mini) in an iBlot 2 Gel Transfer Device (Invitrogen; Carlsbad, CA). Immunodetection was carried out using commercially available primary antibodies against PCCA (1:500 ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were resolved by SDS-PAGE using a 4–15% gradient gel and analyzed by Western blot using the following primary antibodies: mouse anti-ZO1 (Invitrogen, 33–9100) and rabbit anti-Cx34.1 3A4 (Fred Hutch Antibody Technology Facility ...
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bioRxiv - Physiology 2023Quote: ... 40 µg of total protein were run on a SDS-polyacrylamide gel and transferred onto nitrocellulose membranes (GE-Healthcare, Fisher Scientific Italia) After blocking in 5% (w/v ...
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bioRxiv - Cancer Biology 2023Quote: ... Polyadenylation and integrity were assessed by gel electrophoresis in denaturing conditions and RNA was quantified with a Qbit fluorometer (Thermo Fisher Scientific). Purified RNA was resuspended in water at 1-10 μg·mL-1 and stored at -80°C until used.
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bioRxiv - Cell Biology 2023Quote: The eluates from heavy- and light-labeled samples were mixed immediately before loading onto a NuPAGE 4-12% Bis-Tris protein gel (Thermo Fisher Scientific). The gel was stained and visualized using Imperial protein staining reagent (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: pGEX6P1 plasmid DNA was linearized by BamHI and NotI restriction enzymes and further purified with the use of a NucleoSpin Gel and PCR Clean-up kit (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... for in-gel trypsin digestion and MS-seq analysis using a LTQ Orbitrap XL Hybrid Ion Trap-Orbitrap mass spectrometer (Thermo Fisher Scientific). Data were processed using Proteome Discoverer v.1.4 (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2023Quote: ... DAPI and Phalloidin staining was performed by incubating gels for 30 minutes in 2 μg/mL DAPI (Thermo Fisher Scientific, Cat# D1306) and Phalloidin-iFluor 647 (Abcam ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples containing 25 µg of protein were resolved on 8-16% Tris-Glycine or 4-12% Bis-Tris gels (Thermo Fisher Scientific) at 150V for 45 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples of extracts were mixed with sample buffer and DTT and denatured at 70 °C for 10 min before being subjected to SDS-PAGE (60 µg protein/sample lane on NuPAGE 12% Bis-Tris Gel) for ∼4 minutes at 200V 218 mA (∼44 W) in NuPAGE™ MES SDS Running Buffer (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... to be separated on 8% SDS-polyacrylamide gel electrophoresis (PAGE) or subjected to reverse transcriptase-polymerase chain reaction (RT-PCR) using Superscript IV (Invitrogen Life Technologies), to create cDNA and then DNA to amplify for sequencing.
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bioRxiv - Molecular Biology 2023Quote: ... 5-10 µg of total protein was heated at 70°C for 10 min and then resolved on a 4–12% NuPAGE gel (Thermo Fisher Scientific) by SDS-PAGE ...
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bioRxiv - Microbiology 2023Quote: ... Western blotting was performed on 8% SDS-PAGE gels and transferred to polyvinyldifluoride (PVDF) membranes using the iBlot 2 (Thermo Fisher Scientific). Membranes were blocked and probed with purified polyclonal goat antibodies to LiaX diluted 1:3000 and horseradish peroxidase-conjugated mouse anti-goat antibody diluted 1:3000 ...
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bioRxiv - Biochemistry 2022Quote: ... To remove detergents and other buffer components the samples were loaded onto a NuPAGE® 4-12% Bis-Tris Gel 1.0 mm (Life Technologies Corporation). The gel was run for approximately 25 minutes at 200 V ...
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bioRxiv - Bioengineering 2022Quote: ... Proteins were separated by 8% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto polyvinylidene fluoride (PVDF) membrane (Thermo Fisher Scientific). The membranes were blocked in 5% nonfat milk (RPI International ...
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bioRxiv - Genomics 2023Quote: ... lysates and immunoprecipitates were separated on a Bolt 10% Bis-Tris Plus gel and transferred onto a PVDF membrane (both Thermo Fisher Scientific). To determine total EPHB4 amounts and EPHB4 phosphorylation status the following antibodies were used ...
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bioRxiv - Cancer Biology 2022Quote: ... Pre-cast Bolt™ and NuPAGE™ 4-12% Bis-Tris protein gels and 4X LDS sample buffer were purchased from ThermoFisher. Pierce™ BCA Protein Assay Kit was purchased from ThermoFisher ...
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bioRxiv - Cancer Biology 2023Quote: ... Purified PCR products were digested with BlpI and BstXI and the digested fragment was purified on a 20% polyacrylamide Tris–borate–EDTA (TBE) gel (Thermo Fisher Scientific). Digested fragments were purified by isopropanol precipitation ...
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bioRxiv - Cancer Biology 2022Quote: ... boiled for 5min at 100°C and loaded on Novex 4-12% Bis-Tris acrylamide pre-cast gels (#NP0321; Thermo Fisher Scientific) at 170V for about 1h ...
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bioRxiv - Cancer Biology 2022Quote: ... A total of 25-30 μg of total protein lysate per lane were separated on a 4–12% Bis-Tris gel (Thermofisher; Cat# NP0050). Proteins were blotted onto PVDF membranes using iBlot (program 3 for 8 min ...
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bioRxiv - Genomics 2023Quote: All PCR products were assessed by gel electrophoresis and quantified using the Qubit™ dsDNA HS Assay kit (Cat. #Q32854; Thermo-Fisher Scientific). Primers were synthesized by Integrated DNA Technologies® and diluted to 10uM in 0.1x TE buffer ...
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Disruption of the Aspergillus fumigatus RNA interference machinery alters the conidial transcriptomebioRxiv - Microbiology 2023Quote: ... PCR products were loaded onto a 1% agarose gel to check for the correct size and purified with the GeneJET PCR Purification kit (Thermo Fisher Scientific). The products were then used as template for in vitro dsRNA transcription using the MEGAscript RNAi Kit (Invitrogen ...
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bioRxiv - Cancer Biology 2022Quote: ... The resulting peptides were extracted from the gel fragments and analyzed with an Orbitrap Fusion Lumos Tribrid quadrupole-ion trap-Orbitrap mass spectrometer (Thermo Fisher Scientific) combined with an UltiMate 3000 nanoLC system (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2023Quote: ... Digitonin solubilized mitochondria were loaded 3-12% Bis-Tris Invitrogen™ Novex™ NativePAGE™ 3-12% acrylamide gel (1mm) (Invitrogen, BN2011BX10) using the anode running buffer (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: Gel zymography was conducted to measure the enzymatic activity of MMPs using Novex™ 10% Zymogram Plus (Gelatin) protein gels (ZY00105BOX, ThermoFisher Scientific) which contains 10% gelatin ...
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bioRxiv - Biochemistry 2023Quote: ... The samples were separated on a 15% denaturing urea-PAGE and visualized via SYBR™ Gold Nucleic Acid Gel Stain (Invitrogen by Thermo Fisher).
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bioRxiv - Genetics 2023Quote: ... Samples were separated on an 8 12% Bis-Tris SDS-PAGE gel and transferred to a nitrocellulose membrane using the iBlot/iBind system (Thermo Fisher Scientific). The presence of mitochondrial and cytoplasmic ribosomes in gradient fractions was estimated by incubating resulting membranes with anti-MRPL11 monoclonal antibody (D68F2 ...
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bioRxiv - Biophysics 2023Quote: ... in the presence of different concentrations of uranyl acetate (0–1000 µM) were analyzed with blue native polyacrylamide gel electrophoresis (BN- PAGE) using the Invitrogen system (ThermoFisher Scientific, USA). Thus ...
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bioRxiv - Cancer Biology 2023Quote: ... One-third of the eluate was then run on a 10–20% Tris-glycine SDS–PAGE gel and stained with SimplyBlue Coomassie stain (Thermo Fisher Scientific) for 2 h ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were lysed in RIPA lysis buffer and separated on a 4-12% SDS-PAGE gel with Sample Buffer (Life Technologies, NP0007) and the reducing reagent (Life Technologies ...
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bioRxiv - Molecular Biology 2023Quote: ... Amplicons were resolved on 7.6% TBE-acrylamide gels (PanReac Applichem, A1672) at 210V for 20 min and stained with 1:10000 SBYR Safe in H2O (Life Technologies, S33102) for 3 min ...
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bioRxiv - Immunology 2023Quote: ... A total of 20 µg protein was loaded per sample onto a lane of NuPAGE 4-12% Bis-Tris Gel (Thermo Fisher Scientific) in MOPS buffer (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2023Quote: ... All samples were heated at 99 °C for 5 min and separated using Novex Precast 4–20% gels (Invitrogen, Waltham, MA, #WXP42020BOX) in SDS-PAGE ...
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bioRxiv - Immunology 2023Quote: ... to amplify immunoglobulin heavy and light chain variable regions and yielding variable regions were confirmed by using 96 well E-gel agarose electrophoresis (Thermo Fisher Scientific). Purified PCR amplicons were then sequenced with forward and reverse primers and contigs of the amplified immunoglobulin sequences were made by the Duke automated sequence analysis pipeline (Duke ASAP) ...
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bioRxiv - Cancer Biology 2023Quote: ... A total of 25-30 μg of total protein lysate per lane were separated on 4–12% Bis-Tris gels (Thermofisher; Cat# NP0050). Proteins were blotted onto PVDF membranes using iBlot (program 3 for 8 min ...
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bioRxiv - Microbiology 2023Quote: ... The fragments were purified (NucleoSpin Gel and PCR Clean-up Columns, Macherey Nagel) and ligated with T4 DNA ligase (EL0014, Thermo Fisher Scientific). E ...
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bioRxiv - Cell Biology 2023Quote: ... 50μg of protein per sample was separated on 4-20% or 10-20% gradient Novex Tris-glycine polyacrylamide gels (Thermo Fisher Scientific) transferred onto polyvinylidene fluoride membranes (Bio-Rad Laboratories) ...