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Citations for Thermo Fisher :
9001 - 9050 of 9427 citations for Vertical Gel Casting Cassettes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Proteins were separated by the sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) at 150V for 2h using NuPAGE 2-(N-morpholino)ethanesulfonic acid SDS running buffer (NP0002, Thermo Fisher Scientific), NuPAGE Antioxidant (NP0005 ...
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bioRxiv - Cancer Biology 2023Quote: ... protein lysates were loaded onto a precast 4-12% gradient SDS-PAGE gel from the NuPAGE SDS-PAGE system (Invitrogen, Carlsbad, CA). The gel was run at 200 V for 40 min. ...
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bioRxiv - Physiology 2023Quote: ... The gel was then electroblotted onto a nitrocellulose membrane using iBlot 2 dry blotting system (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA). Non-specific sites on the membrane were blocked with 5 % milk powder in 1X TBST [10 mM Tris (pH 7.5) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Pooled samples of protein extracts (80 μg) were separated using electrophoresis on 4– 12% NuPage Bis-Tris gels with MOPS buffer (Invitrogen, Carlsbad, CA) and then transferred to Immobilon-P membranes (Millipore ...
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bioRxiv - Cancer Biology 2023Quote: ... A total of 25 µg for each sample was loaded on a NuPAGE 4-12% Bis-Tris gel with 1X NuPAGE MOPS Running buffer (Life Technologies, Invitrogen) and separated for 1.5 h at 120V ...
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bioRxiv - Plant Biology 2023Quote: ... Quantitative RT-PCR was performed in 1 μL of one-fifth diluted cDNA in 35-μL reactions containing the SYBR™ Green I Nucleic Acid Gel Stain (1:20,000 final dilution; Thermo Fisher Scientific), Taq DNA polymerase (1.75 units ...
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bioRxiv - Plant Biology 2023Quote: ... The quality and quantity of isolated RNA samples were assessed by performing 1% agarose gel electrophoresis and NanoDrop 2000C (Thermo Fisher, USA). The high-quality RNA samples were outsourced for RNA sequencing ...
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bioRxiv - Cell Biology 2023Quote: ... or 10μg EV proteins were separated by sodium dodecylsulfate-polyacrylamide gel electrophoresis (10-12% polyacrylamide, SureCast™ Acrylamide Solution, ThermoFisher Scientific, MA) and transferred onto PVDF membranes ...
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bioRxiv - Cell Biology 2023Quote: ... 40 µg of total protein were run on an SDS-polyacrylamide gel and transferred onto nitrocellulose membranes (GE-Healthcare, Fisher Scientific Italia) After blocking in 5% (w/v ...
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bioRxiv - Neuroscience 2023Quote: ... Material from 25 000 to 50 000 nuclei were loaded on NuPAGE™ 4 to 12% Bis-Tris Mini gels (ThermoFisher, #NP0322BOX), aiming for equal loading in each well ...
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bioRxiv - Immunology 2023Quote: ... Cell lysates were separated by gel electrophoresis using a 4%-12% gradient cell (Thermo Cat# NW04120BOX) or 12% gel (Thermo Cat# NW00127BOX) and then transferred to a 0.2 μm pore-size nitrocellulose membrane (Thermo Fisher Cat# LC2000). The membranes were blocked using 10% nonfat dry milk solution (Fisher Scientific Cat# 50-488-786 ...
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CD56/NCAM mediates cell migration of human NK cells by promoting integrin-mediated adhesion turnoverbioRxiv - Cell Biology 2023Quote: ... 5 × 105 cell equivalents per well were loaded into a NuPAGE 4-12% Bis-Tris density gradient gel (Thermo Fisher Scientific; NP0432) and ran at a constant 150V for 80 minutes ...
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bioRxiv - Microbiology 2023Quote: ... from the six macaques immunized with the highest dose of DENVLP were pooled together and purified using Melon Gel IgG Spin Purification Kit and NAb Spin column (ThermoFisher #45206, #89961) followed by buffer exchange to PBS via Slide-A-Lyzer dialysis cassettes (ThermoFisher #66012 ...
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bioRxiv - Neuroscience 2023Quote: ... 20 µg of the protein samples were then heat-denatured and subsequently separated on a 4-12% Bis-Tris gel (Invitrogen; Thermo Fisher Scientific). Separated proteins were then transferred to a 0.45 µm nitrocellulose membrane via wet blotting for 3h at 300 mA ...
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bioRxiv - Molecular Biology 2023Quote: ... at 70°C for 10 min and then electrophoretically separated by SDS-PAGE in 4-12% Bis-Tris precast gels in MOPS buffer (Invitrogen/Thermo-Fisher). Gels were transferred to PVDF membranes using the iBlot2 semi-dry transfer device (Invitrogen/Thermo-Fisher ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The samples were first heated for 5 min at 95°C and then centrifuged at 11,000xg in microfuge before loading them on 4-12% pre-cast mini-or midi-gels (Thermo-Fisher Scientific/Life Technologies). The running buffer contained MOPS [3-(N-morpholino ...
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bioRxiv - Neuroscience 2023Quote: SDS-PAGE was conducted according to Laemmli (Laemmli, 1970) using 5-20% gradient gels in a Hoefer™ Mighty Small System (Fisher Scientific). For each sample a total of 10 µg protein was loaded and separated at a voltage of 12 mA per gel and a constant temperature of 4°C ...
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bioRxiv - Microbiology 2023Quote: ... Bradford assay was used to determine the total protein concentration in each sample so that an equal amount of total proteins were loaded on each well of a 4-20% Novex WedgeWell SDS-PAGE gels (Thermo Fisher Scientific). Resolved proteins were transferred to PVDF membranes using the Trans-Blot Turbo Transfer System (BioRad) ...
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bioRxiv - Genetics 2023Quote: A pooled equimolar mixture of the ChIP-ISO synthetic oligonucleotide library was PCR-amplified and purified via agarose gel purification (Thermo Scientific K0691). The EMSA protocol was adapted from Garcia et al ...
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bioRxiv - Microbiology 2023Quote: ... and their purity validated by sodium dodecyl sulfate -polyacrylamide gel electrophoresis (SDS-PAGE) followed by Western blot (WesternBreeze Chromogenic Immunodetection System, Thermo Fisher Scientific) using antibodies against His6xtag.
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bioRxiv - Genomics 2023Quote: ... and 20 μL of the mix was loaded onto a well on a 2% Agarose E-gel (Thermo Fisher Scientific, Cat # G401002). Next ...
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bioRxiv - Cell Biology 2023Quote: ... were boiled for 5 min and subjected to SDS-PAGE using NuPAGE™ 4-12% Bis-Tris Gels (Invitrogen; Thermo Fisher Scientific) at 120V for 80 min and transferred to 0.45 µm PVDF membranes (Sigma ...
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bioRxiv - Cancer Biology 2023Quote: ... Reduced samples (by addition of 2-mercaptoethanol) were heated at 95°C for 5 minutes then loaded onto a 4-12% tris-glycine gradient gel (Thermo Fisher, XP04122BOX) and ran at 125 volts for 1-1.5 hours ...
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bioRxiv - Cell Biology 2023Quote: ... Samples denatured by 3 min boiling in Laemmli buffer were separated by electrophoresis on 4-12% bis-acrylamide gel (Thermo Fisher Scientific), then transferred to 0.45 µm nitrocellulose membranes (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... see the “PAA elastic gel premix” section) of the projected rigidity G’ = 50 kPa(75) with the addition of the 5% streptavidin-acrylamide (ThermoFisher, Cat# S21379) were ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were run on 4-12% Bis-Tris polyacrylamide gels (Novex, NP0322) in NuPage® SDS MOPS running buffer (ThermoFisher Scientific, NP0001). Proteins were then electro-transferred onto polyvinylidene fluoride (PVDF ...
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bioRxiv - Cell Biology 2023Quote: ... were boiled for 5 min and subjected to SDS-PAGE using NuPAGE™ 4-12% Bis-Tris Gels (Invitrogen; Thermo Fisher Scientific) at 120V for 80 min and transferred to 0.45 µm PVDF membranes (Sigma ...
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bioRxiv - Developmental Biology 2023Quote: ... and equal amounts of proteins (∼20 µg) were resolved on NuPAGE 4–12% Bis-Tris gels (Novex® by Invitrogen™ - WG1401BX10) under reducing conditions ...
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bioRxiv - Cancer Biology 2023Quote: ... Equal amounts of protein lysates (10 μg/lane) were fractionated on a NuPAGE Novex 4%–12% Bis-Tris Protein Gel (Thermo Fisher Scientific) and transferred onto a PVDF membrane (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2023Quote: ... was added to the ribosome samples followed by separation on 8% (w/v) Bolt Bis-Tris gel in MES buffer (Thermo Fisher Scientific), followed by transfer onto a 0.45 μm PVDF Immobilon-P membrane (Bio-Chapter 691Rad) ...
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bioRxiv - Molecular Biology 2023Quote: ... and DNA integrity was confirmed using a 2% agarose gel stained with GelRed ® and DNA purity was checked with Nanodrop 6000 (Thermo Fisher). The gDNA generated was sent to Novogene ...
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bioRxiv - Molecular Biology 2023Quote: ... A total of 20 µg of protein lysate was loaded onto 4- 12% Bis-Tris Plus Mini protein gels (ThermoFisher, Cat # NW04122BOX) and transferred onto nitrocellulose membranes (Bio-Rad ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were then centrifuged at 14000 g for 10 min and loaded onto a NuPAGE Novex 4-12% Bis-Tris gel (Novex Life Technologies, WC1020) and run at 120 V for 90 minutes ...
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bioRxiv - Synthetic Biology 2024Quote: ... RNA integrity was verified with an agarose gel and 260/280 and 230/280 ratios were measured with a nanodrop 1000 (Thermo Fisher Scientific). RNAseq was performed by BGI Tech Solutions (Hong Kong ...
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bioRxiv - Immunology 2024Quote: ... 67 or Bacillus anthracis were loaded to 1-well 4-12% Bis-Tris NuPAGE Novex SDS-PAGE gels (Invitrogen, Thermo Fisher Scientific). Proteins were separated for 45 min at 200 V ...
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bioRxiv - Biochemistry 2024Quote: ... the samples were transferred into fresh tubes and 50 µg of each sample was subjected to native gel electrophoresis to reveal the various proteasome complexes (30S, 26S, 20S) using a NativePAGE™ 3 to 12% Bis-Tris gel (Invitrogen, BN1001BOX) in XCell™ SureLock™ Mini-Cell system (Invitrogen) ...
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bioRxiv - Neuroscience 2024Quote: ... The purity of viruses was routinely checked by silver staining (Pierce, Germany) after gel electrophoresis (NovexTM 4-12% Tris-Glycine, Thermo Fisher Scientific).
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Entorhinal cortex vulnerability to human APP expression promotes hyperexcitability and tau pathologybioRxiv - Neuroscience 2024Quote: To confirm protein enrichment on the pulldown samples the protein were eluted from the beads as mentioned above and 1/3rd of the eluted protein was resolved on a 4–12% Bris-Tris gel (Invitrogen: cat# NW04125box) and transferred onto transferred onto iBlot 2 Transfer Stack containing nitrocellulose membrane using the BOLT transfer system ...
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bioRxiv - Microbiology 2024Quote: Five - 10mg of protein from the cell lysates were run on 4-12% NuPAGE Bis-Tris SDS-PAGE gels (Invitrogen by ThermoFisher Scientific®) and transferred to PVDF membranes (ThermoFisher Scientific® ...
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bioRxiv - Cancer Biology 2024Quote: ... Equal amounts of protein (30 μg) were resolved on 12% SDS– polyacrylamide gel electrophoresis and transferred to 0.45 um pores PVDF membranes (Thermo Fisher Scientific, 88518). The membranes were blocked in 5% nonfat dry milk in TBST [50 mM tris ...
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bioRxiv - Plant Biology 2024Quote: ... immunoprecipitated proteins were collected on SDS–PAGE gels and the samples were analyzed using High Resolution LC/MSMS spectrometer (Q Exactive, Thermo Scientific, USA) in NICEM (National Instrumentation Center for Environmental Management ...
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bioRxiv - Bioengineering 2024Quote: ... Proteins were separated by dodecyl-sulfate polyacrylamide gel electrophoresis and transferred to Amersham™ Protran® nitrocellulose membranes or PVDF membranes (Invitrogen). The membranes were blocked with 5% non-fat dry milk-TBST (10 mM Tris-HCL [pH 8.0] ...
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bioRxiv - Bioengineering 2024Quote: ... 1 % RGD and 5 % RGD gels were formed to volumes of 500 μL in black 96-well MicroWell plates (Nunc, Roskilde, Denmark), and incubated for 24 hours ...
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bioRxiv - Neuroscience 2024Quote: ... The purity of produced viruses was routinely checked by silver staining (Pierce, Germany) after gel electrophoresis (NovexTM 4–12% Tris-Glycine, Thermo Fisher Scientific) according to the manufacturer’s instructions viral stocks were kept at −80°C until the injection day.
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bioRxiv - Neuroscience 2024Quote: ... Proteins present in supernatant were solubilized with Laemmli sample buffer and 40 µl of each sample were resolved by NuPAGE 4-12% Bis-Tris gel (MES buffer, Thermo Scientific, WG1401) and transferred onto nitrocellulose membranes ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were separated on a 3 – 12% polyacrylamide gradient gel (Serva, #43250) alongside a molecular weight marker (NativeMark, Thermo Fisher Scientific, #LC0725). After electrophoresis ...
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bioRxiv - Neuroscience 2024Quote: ... Brain extracts containing 60 ug of protein were loaded and separated on 6-10% denaturing polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to polyvinylidene difluoride (PVDF) membrane (Cat#88518; Thermo Fisher Scientific). The membrane was blocked with 5% milk in Tris-buffered saline with 0.1% Tween (TBST ...
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bioRxiv - Cancer Biology 2024Quote: ... and 10-30 μg of proteins were loaded and resolved on either a 10% or 4-12% Bolt Bis-Tris gel (Thermo Fisher Scientific), transferred to a nitrocellulose membrane (Bio-Rad) ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were heated at 100°C for 10 minutes and loaded on a NuPAGE 4%–12% Bis-Tris gel (Thermo Fisher Scientific). Afterward ...
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bioRxiv - Genomics 2024Quote: The quality-compliant DNA was further tested for purity and integrity using 0.8% agarose gel electrophoresis and a Qubit dsDNA HS Assay Kit on a Qubit 3.0 Fluorometer (Life Technologies, Carlsbad, CA, USA). RNA contamination was identified using a 1.5% agarose gel ...