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Citations for Thermo Fisher :
9201 - 9250 of 9427 citations for Vertical Gel Casting Cassettes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... An equivalent of 2×107 parasites whole protein lysate was loaded on a NuPAGE 4-12% Bis-Tris protein gel (Invitrogen, cat log # NP0321BOX) followed by transfer onto a PVDF membrane (Thermo Scientific ...
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bioRxiv - Microbiology 2024Quote: ... The extracted DNA was electrophoresed on a 1% agarose gel and checked with a NanoDrop 2000 UV-vis spectrophotometer (Thermo Scientific, Wilmington, USA). PCR reactions were performed as described in a previous paper (Shen et al. ...
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bioRxiv - Neuroscience 2024Quote: ... A total of 30 μg of protein fraction for each sample was separated by electrophoresis using NuPAGETM 4-12% Bis-Tris Protein Gel (ThermoFisher Scientific, Waltham, MA). Proteins were transferred to a polyvinylidene difluoride (PVDF ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell lysates (20 μg) were analyzed via electrophoresis using 4%-12% sodium dodecyl sulphate-polyacrylamide gradient gels (Thermo Fisher Scientific, Waltham, MA, USA). Pre-stained protein ladders (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... lysates were extracted with Laemelli sample buffer for 10 minutes at 75°C and lysates were run on 4% to 12% Bis-Tris gels (Invitrogen; Thermo Fisher Scientific). Proteins were transferred to nitrocellulose membranes (Prometheus ...
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bioRxiv - Neuroscience 2024Quote: ... for each sample were loaded onto 10% polyacrylamide gels and separated by electrophoresis and transferred overnight to nitrocellulose membranes (Thermo Fisher, Stockholm, Sweden). The membranes were immunoblotted with primary antibodies against actin (1:30,000 ...
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bioRxiv - Immunology 2024Quote: ... Purity of both mouse and human was confirmed by running purified THP on a 4-12% Bis-Tris polyacrylamide gel and staining with SimplyBlue™ SafeStain (Thermo Fisher Scientific). THP was visualized as a single band at ∼ 85 kDa (Supp ...
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bioRxiv - Genomics 2024Quote: ... 100 ng of each probe set was then analyzed on a 12.5% Urea-PAGE gel stained with SYBR-Gold (Thermo Fisher Scientific, Waltham, MA).
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bioRxiv - Cancer Biology 2024Quote: ... Lysates were boiled in LDS loading buffer with 0.1M DTT and run on 4-12% precast Bis-Tris gels (NP0336BOX, Thermo Fisher Scientific, Waltham, MA). Proteins were transferred using an iBlot2 transfer device (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... Gel slices of RNA fragments with sizes between 26-34 nt were excised from a 15% polyacrylamide TBE-Urea gel (Thermo Fisher Scientific, EC68855BOX). Eluted RNA was treated with T4 PNK and preadenylated linker was ligated to the 3’ end using T4 RNA ligase 2 truncated KQ (NEB ...
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bioRxiv - Immunology 2022Quote: ... Fractions were pooled based on A280 signals and SDS-PAGE analysis on 4-20% Mini-PROTEAN® TGX™ protein gels stained with GelCode™ Blue Stain Reagent (ThermoFisher). Prior to immunizations or freezing ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were heated to 95 °C for 5 min prior to SDS-PAGE gel electrophoresis (NuPAGE MES SDS running buffer, Thermo Fisher Scientific, #NP0002). Proteins were transferred to a nitrocellulose membrane (#1704158 ...
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bioRxiv - Molecular Biology 2023Quote: ... 2019 and from callus tissues using RNeasy Plant Mini Kit (Qiagen).The quality and concentration of extracted RNA were checked with 1 % agarose gel electrophoresis and estimated with Multiskan Sky Microplate Spectrophotometer (Thermo Fisher Scientific, USA), respectively ...
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bioRxiv - Neuroscience 2023Quote: ... A total of 30 μg protein was separated by SDS-PAGE at 200 V for 45 min using a 4%–12% precast polyacrylamide gel (Novex; Invitrogen, Carlsbad, CA, USA), then transferred to polyvinylidene fluoride membranes (Millipore ...
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bioRxiv - Synthetic Biology 2023Quote: ... The first-round PCR product was purified and eluted into 20 µL of ddH2O using GeneJET Gel Extraction Kit (Thermo Fisher Scientific #K0691). The second-round PCR was then reamplified in 20 µL volume ...
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bioRxiv - Biochemistry 2022Quote: All NativePAGE analyses were conducted in an Invitrogen XCell SureLock using NativePAGE™ 4 to 16% bis-tris mini protein gels and NativeMark Unstained Protein Standard from Fisher Scientific (USA) with 1x running buffer made from 10x Tris/Glycine Buffer from Bio-Rad Laboratories ...
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bioRxiv - Molecular Biology 2022Quote: ... treated with 100 μl stain mix (PBS, 1mg/ml RNase, 1/1000 SYBR™ Green I Nucleic Acid Gel Stain, Thermo Fisher Scientific) at 37°C for 30 min ...
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bioRxiv - Cell Biology 2023Quote: ... Samples (10 µg) were then added to each well and protein complexes were separated on 3-12% NativePAGE gels (Thermo Fisher Scientific, BN1003BOX). Proteins were transferred to PVDF membranes and probed with specific antibodies ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were separated by sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to polyvinylidene fluoride (PVDF) membranes (EMD Millipore; Thermo Fisher Scientific). PVDF membrane was treated with primary and secondary antibodies ...
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bioRxiv - Microbiology 2023Quote: ... Samples were analyzed in 15 well 1.0 mm Bolt 4-12% Bis-Tris Plus mini protein gels (Thermo Fisher Scientific, Waltham, MA, USA) and stained with Coomassie blue G-250.
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bioRxiv - Immunology 2023Quote: The IgG antibodies were extracted from a pool of 100 healthy human serum samples using the Melon™ Gel IgG Spin Purification Kit (Thermo Fisher Scientific), with a tandem purification (one after another ...
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bioRxiv - Molecular Biology 2023Quote: ... IMR90 EVs and concentrated CM from various cell lines were denatured at 70°C for 10 minutes and run on NuPAGE® Novex® 4-12% Bis-Tris gel system (Invitrogen, NP0321BOX). Gels were electroblotted onto the nitrocellulose membrane (Amersham Pharmacia Biotech) ...
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bioRxiv - Physiology 2023Quote: ... Proteins were denatured at 95°C for 5 minutes and run on a Bolt™ 4-12% Bis-Tris polyacrylamide gel (Thermo Scientific #NW04125BOX). Proteins were transferred to a nitrocellulose membrane (Invitrogen #PB3310 ...
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bioRxiv - Bioengineering 2023Quote: ... The resulting PCR products were gel purified and quantified with Nanodrop and Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific, cat. no. Q32851). Pooled guide libraries were sequenced on Illumina NextSeq ...
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bioRxiv - Physiology 2023Quote: ... hTERT-HM cells were suspended in a collagen gel matrix (Cellmatrix collagen type I-A, Fisher Scientific, prepared with MEM, NaOH and HEPES) then plated in 12-well dishes and grown in hTERT-HM medium ...
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bioRxiv - Cell Biology 2023Quote: ... 4-12% NuPAGETM Bis-Tris precast polyacrylamide protein gels were used with 1x NuPAGETM MES SDS Running Buffer (Thermo Fisher Scientific, MA, USA). The samples were prepared with NuPAGETM LDS Sample Buffer (4X ...
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bioRxiv - Cancer Biology 2023Quote: ... Gel lanes between 10-300 kDa were excised into five-six pieces and subjected to in-gel digestion using sequencing-grade trypsin (Thermo Scientific, Cat# 90057), as previously described 65 ...
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bioRxiv - Cancer Biology 2023Quote: ... PCR products were diluted 1:10 before loading on a 1% agarose gel alongside GeneRuler DNA Ladder Mix (Thermo Scientific, Cat. No. SM0331).
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bioRxiv - Microbiology 2023Quote: ... Samples were then run on SDS-PAGE on a 4-12 % NuPAGE® Bis-Tris mini gel in a XCell SureLock® Mini-Cell filled with MES SDS running buffer (Invitrogen, USA) until samples just entered the gel ...
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bioRxiv - Molecular Biology 2023Quote: ... Amplified DNA fragments were resolved by electrophoresis in 1% agarose gels alongside GeneRuler 1 kb Plus DNA Ladder (Thermo Fisher Scientific, cat# SM1331). CRISPR/Cas9-induced mutations in AS-NMD-regulated genes were characterized with primers designed to amplify either short (∼0.5 kb ...
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bioRxiv - Biochemistry 2023Quote: ... of centrifugation (12,000 rpm at 4 °C for 10 min)-cleared whole-cell lysate were loaded per lane in a commercial 3-8% 1.5-mm x 15-well Tris-Acetate SDS gel (Thermo Fisher Scientific, Catalog # EA03785BOX) with 1 x NuPAGE Tris-Acetate SDS Running Buffer (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2022Quote: ... a 1/200 dilution of cells were transferred to fresh media containing 1X SYBR Green I Nucleic Acid Gel Stain (Invitrogen, now Theremo Fisher Scientific, Loughborough,UK) and FACS-counted (FACScan ...
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bioRxiv - Bioengineering 2023Quote: ... For each sample 2 μg of solubilized silk protein was loaded into a NuPAGE™ 7% Tris-Acetate gel (EA03585BOX, Invitrogen, Waltham, MA) under reducing conditions ...
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bioRxiv - Microbiology 2022Quote: ... The amplicons were extracted and purified from the gel using the Zymo gel extraction kit according to the manufacturer’s instructions and quantified using the Quant-iT dsDNA Assay Kit (Thermo Fisher Scientific, MA, USA). Finally ...
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bioRxiv - Neuroscience 2023Quote: ... and 0.005 % BPB) and incubated 5 min at 100 ºC followed by electrophoresis on Novex™ 4-20% Tris-Glycine gradient gels (Invitrogen, WXP42020BOX; WXP42026BOX). Proteins were transferred onto 0.2 µm nitrocellulose membranes (Pall Laboratory ...
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bioRxiv - Cell Biology 2023Quote: 25µg of total cell extracts or of purified nucleoli extracts were run of an 4%-20% gradient acrylamide gel (Novex™ WedgeWell™, Tris-Glycine, Invitrogen #XP04200BOX) and transferred on nitrocellulose membrane via semi-dry transfer ...
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bioRxiv - Cell Biology 2023Quote: ... the samples were loaded into 12% Tris-Glycine Precast Gels (Pierce, 25247) and stained with Page Blue Protein Staining (Thermo Fisher Scientific, 24620). The band corresponding to Keratin 8:GST (66-81 kDa ...
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bioRxiv - Cancer Biology 2023Quote: ... 30 μg of total protein were loaded on RunBlue 4-12% Bis-Tris gels (GeneScript: M00656) supplemented with 4x NuPage LDS Sample buffer (Thermo Fisher Scientific: NP0007) and 10 mM DTT (Sigma-Aldrich ...
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bioRxiv - Immunology 2022Quote: ... The molecular weight and the supercoiled/open coiled ratio of the pDNA were analyzed by running 200 ng of plasmid DNA on each lane on a 1% agarose TBE gel stained with SYBR-Safe DNA (Thermo Fisher Scientific,USA) for 2 h at 45 V ...
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bioRxiv - Evolutionary Biology 2023Quote: ... DNA quality and concentration were assessed by electrophoresis on a 2% agarose gel and a Qubit 4.0 Fluorometer (Thermo Scientific, Waltham, MA, USA). DNA sequencing libraries were prepared following standard MGI sequencing library protocol by the Beijing Genomics Institute (BGI ...
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bioRxiv - Genetics 2023Quote: ... Separated proteins were transferred onto a nitrocellulose membrane using an iBlot™2 Gel transfer device (cat. IB21001, Thermo Fisher Scientific, IL, USA). Non-specific protein binding to the membranes was blocked with 5% non-fat dry milk (cat ...
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bioRxiv - Synthetic Biology 2023Quote: ... the amplicons were separated in a 1% agarose gel after electrophoresis at 140 V and identified by size based on gene ruler Plus DNA Ladder (Thermo Fisher Scientific, Lithuania). Amplicons were purified using Favorprep™ GEL/PCR Purification Kit (Favorgen ...
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bioRxiv - Molecular Biology 2023Quote: ... The resulting peptides were extracted from the gel pieces and analyzed using an Orbitrap Fusion Lumos mass spectrometer (Thermo Fisher Scientific, Waltham, MA) in combination with an UltiMate 3000 RSLC nanoflow HPLC (Thermo Fisher Scientific) ...
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bioRxiv - Plant Biology 2023Quote: ... Equal amount of protein samples were electrophoresed on 10 % NuPAGE® Bis-Tris Gels in 1X MES [2-(N-morpholino) ethane sulfonic acid] SDS buffer (Invitrogen, Waltham, MA) and transferred to a nitrocellulose membrane (BioRad ...
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bioRxiv - Evolutionary Biology 2023Quote: ... 5 μl of product from 35 rounds of PCR were analyzed for each time-point in by 2% agarose gel electrophoresis (Top Vision, Thermo Scientific, Waltham, MA). Each result is representative of a minimum of two independent replicates.
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bioRxiv - Biochemistry 2023Quote: ... 15 μgs of centrifugation (12,000 rpm at 4 °C for 10 min)-cleared whole cell lysate were loaded per lane in a commercial 3-8% 1.5-mm x 15- well Tris-Acetate SDS gel (Thermo Fisher Scientific, Catalog # EA03785BOX), electrophoresing with 1 x NuPAGE Tris-Acetate SDS Running Buffer (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2023Quote: A hydrogel sample of 5 μm FFPE mouse liver was stained through SYPRO Ruby protein gel stain (Thermo Fisher Scientific, the United States) for 30 minutes ...
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bioRxiv - Plant Biology 2023Quote: ... total proteins from pollen samples were separated on a 10% SDS-PAGE gel containing the PageRuler™ Prestained Protein Ladder as a molecular weight marker (Thermo Fisher Scientific). After in-gel digestion with trypsin (Promega) ...
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bioRxiv - Microbiology 2023Quote: ... 5 μL of the IP fraction for α-FLAG immunoblots were loaded on a 4-20% Novex WedgeWell SDS-PAGE gels (Thermo Fisher Scientific). Resolved proteins were transferred to polyvinylidene fluoride (PVDF ...
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bioRxiv - Biochemistry 2023Quote: ... The resulting supernatant containing soluble CCTX2 was then filtered through a 0.22 μm PES membrane filter and loaded onto a 5 mL Tricorn column packed with immobilized D-galactose gel (Pierce™, Thermo Scientific™) previously equilibrated with 6 CV loading buffer (20 mM Na-HEPES ...