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Citations for Thermo Fisher :
9301 - 9350 of 9427 citations for Vertical Gel Casting Cassettes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... full-length VZV gB was resolved on Native PAGE gels and the single protein band that migrated to 690kDa was excised from the gel to be processed for mass spectrometry using an Orbitrap Fusion Mass Spectrometer (Thermo Scientific, San Jose, CA). Gel samples were diced into 1 mm cubes ...
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bioRxiv - Molecular Biology 2020Quote: ... The quality and concentration of the extracted DNA were assessed using 1% agarose gel electrophoresis with comparison to known quantities of standard λ phage DNA (Invitrogen, Carlsbad, CA, USA).
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bioRxiv - Molecular Biology 2020Quote: ... unit of cells in a 1X Laemmli/8 M Urea/ 2.5 % β-mercaptoethanol sample buffer and incubated for 10 minutes at 70 °C prior to SDS-PAGE using 4-12 % Bis-Tris gels (Invitrogen NuPAGE®, Thermo Scientific) in a MOPS buffer (Invitrogen NuPAGE® ...
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bioRxiv - Microbiology 2021Quote: ... Samples were separated by SDS-PAGE (NuPAGE™ 4-12% Bis-Tris Protein Gels, Invitrogen and then transferred to a PVDF transfer membrane (Thermo Scientific, 0.2 μM) following standard procedures [64 ...
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bioRxiv - Microbiology 2021Quote: ... Samples were then run on 4%–12% Bis Tris gels and transferred onto nitrocellulose or PVDF membranes using an iBlot or semidry (Life Technologies and Biorad, respectively).
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bioRxiv - Molecular Biology 2022Quote: ... Three out of 12.5 μl of the in-gel digested sample were loaded onto a fused silica trap column (Acclaim PepMap 100, 75 μmx2cm, Thermo Fisher Scientific, Waltham, MA). After washing for 5 minutes at 5 μl/min with 0.1% TFA ...
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bioRxiv - Microbiology 2022Quote: ... Equal amounts of protein samples were separated using 4-12% NuPAGE™ Bis-Tris gel in 1× NuPAGE™ MES buffer or MOPS buffer (Thermo Fisher Scientific). Proteins were transferred from the gel using the iBlot® 2 transfer stacks (Life Technologies ...
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bioRxiv - Genomics 2022Quote: ... DNA quality was checked by electrophoresis on 1% agarose gels and DNA concentration was measured using a Qubit® dsDNA HS Assay Kit in Qubit® 2.0 Fluorometer (Life Technologies, CA, USA).
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bioRxiv - Molecular Biology 2022Quote: ... The DNA quality was checked and concentration was determined by electrophoresis in a 1% (w/v) agarose gel stained with ethidium bromide and using Gene Ruler 1 kb DNA Ladder (Thermo Scientific, Waltham, MA, USA) as standard.
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bioRxiv - Pathology 2019Quote: ... The quality of DNA was checked using agarose gel (1%) electrophoresis and quantified with Nanodrop™ and UV-Vis Spectrophotometer (Thermo Fisher Scientific, USA).
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bioRxiv - Biophysics 2019Quote: ... Both the biotinylated and intracellular fractions were normalized to total protein and loaded on a 4-12% Bis-Tris pre-cast gel (Thermo Fisher Scientific, Invitrogen, Carlsbad, CA USA) and run at 200 V for 30 min ...
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bioRxiv - Microbiology 2019Quote: ... The quality of DNA (average molecular size) was controlled on 1% (w/v) agarose gels stained with 1× SYBR® Safe ethidium bromide (Invitrogen, Grand Island, NY) and its quantity was measured using the dsDNA HS Assay kit for Qubit fluorometer (Invitrogen).
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bioRxiv - Plant Biology 2019Quote: ... 50 mg mL−1 Serva Blue G) and applied to the precast native 3-12% Bis-Tris polyacrylamide gel (Invitrogen, Life Technologies Corporation, Carlsbad, CA). Blue Native gel electrophoresis was run according to Rantala et al.52 ...
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bioRxiv - Bioengineering 2019Quote: ... Then a fluorescence image of the gel was acquired and the intensity of the RNA band was quantified against an RNA ladder (low Range Riboruler, Thermo Fisher Scientific, No. SM1831) with ImageJ (Fig S4c).
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bioRxiv - Bioengineering 2019Quote: ... Equivalent amounts of lysates (20 μg total protein per lane) were loaded and separated by 10% SDS-PAGE (Invitrogen Bolt Bis-Tris Plus gel). Then ...
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bioRxiv - Pathology 2020Quote: ... The DNA samples extracted from fungal mycelium were visualized on 1% agarose gels stained with SYBR Green under ultraviolet light and quantified using spectrophotometry with a NanoDrop 2000 (Thermo Scientific, Pittsburgh, PA, USA).
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were transferred onto nitrocellulose membranes using either the BioRAD Semi-Dry Blot system (30 minutes in 1xBjerrum buffer + 20 % methanol) for SDS-PAGE gels or the iBlot2® (P0, 7mins, using iBlot® 2NC regular stacks) (Invitrogen, Thermo Fisher Scientific) for pre-casted gels ...
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bioRxiv - Physiology 2021Quote: ... the purity and integrity of dsRNA were confirmed by running a 1.5 % agarose gel and quantified using NanoDrop™ (Thermo Fisher Scientific, Massachusetts, US).
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bioRxiv - Microbiology 2020Quote: ... Peptides were extracted from the gels and analysed by LC-MS/MS on an Orbitrap-Fusion™ mass spectrometer (Thermo Fisher, Hemel Hempstead, UK) equipped with an UltiMate™ 3000 RSLCnano System using an Acclaim PepMap C18 column (2 µm ...
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bioRxiv - Microbiology 2020Quote: ... The quality of extracted DNA was assessed by visualization on a 1% agarose gel with a high molecular mass ladder (Invitrogen, ThermoFisher Scientific, LLC, USA).
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bioRxiv - Genomics 2019Quote: ... DNA quality was assessed by electrophoresis on 3% agarose gels and DNA concentration was determined by dsDNA HS Qubit Assay (Molecular Probes by Life Technologies, No. Q32851). The Center for Genome Research and Biocomputing at Oregon State University generated 94 Wafergen DNA 150bp paired-end libraries using the Biosystems Apollo 324 NGS library prep system ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The amplicons were separated using the agarose gel-electrophoresis and cloned using TOPO TA Cloning Kit for Sequencing (Thermo Fisher Scientific K.K., Tokyo, Japan) following the manufacture’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... PCR products were purified using the GeneJET Gel Extraction Kit (ThermoFisherScientific, Vilnius, Lithuania) and quantified using a fluorometric RiboGreen kit (Qubit Assay Kit, Invitrogen, Life Technologies Corporation, Oregon, USA) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: Samples were resolved in NuPAGE™Novex™ polyacrylamide gels and proteins were transferred to nitrocellulose membranes with the iBlot system (all from Thermo Fisher Scientific). Indicated primary antibodies were incubated overnight at 4°C diluted in Tris-buffered saline containing 0.1% Tween-20 and 5% BSA ...
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bioRxiv - Plant Biology 2021Quote: ... fragments were purified and used as templates for in vitro transcription. The products of RNA transcripts were confirmed by gel electrophoresis (Supplementary Fig. S1) and quantified by NanoDrop (Thermo Fisher Scientific, Waltham, USA). We equally mixed 3 fragments and used for SIGS assay on polyethylene tape ...
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bioRxiv - Cell Biology 2020Quote: ... n-dodecyl-β-D-maltoside and assessed for OxPhos complex content using a Native PAGE Novex Bis-Tris Gel system (Invitrogen, Carlsbad, CA, USA). The separated proteins were transferred to polyvinylidene fluoride membranes ...
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bioRxiv - Plant Biology 2020Quote: ... for 1 hour and the TCEP gel was then removed by passing the sample through a plastic column fitted with a porous disc (Thermo Scientific, Cat number 89898). NEM was then added to a final concentration 20 mM followed by incubation at room temperature in the dark for 2 hours ...
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bioRxiv - Microbiology 2021Quote: ... Samples were then run on 4%–12% Bis Tris gels and transferred onto nitrocellulose or PVDF membranes using an iBlot or semidry (Life Technologies and Biorad, respectively).
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bioRxiv - Evolutionary Biology 2020Quote: ... DNA was first quality-checked by gel electrophoresis and then quantified with a PicoGreen fluorometer quantitator and Quant-iT™ PicoGreen™ dsDNA Assay Kit (Invitrogen™, ThermoFisher Scientific). The Genomic Sequencing and Analysis Facility (GSAF ...
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Genome Assembly of the Roundjaw Bonefish (Albula glossodonta), a Vulnerable Circumtropical SportfishbioRxiv - Genomics 2021Quote: ... We examined the quality of DNA extractions visually using gel electrophoresis and by quantifying isolated DNA using a Qubit fluorometer (Life Technologies, Carlsbad, California, USA) [46].
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bioRxiv - Genetics 2019Quote: ... Samples were then boiled at 90C for 5 minutes and centrifuged before being loaded into a 4-12% Bis-Tris protein gel (Thermo Fisher Scientific, cat #NP0322) and run with MOPS running buffer (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2021Quote: ... Three μl of each library was run on a 1% agarose gel with GeneRuler 1 kb Plus DNA Ladder (ThermoFisher Scientific; Cat. No. SM1331) followed by quantification via Qubit™ dsDNA BR Assay Kit (Invitrogen ...
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bioRxiv - Neuroscience 2022Quote: ... Supernatant protein (20 μg) was loaded in a 10%-Tris-Glycine gels and transferred to a nitrocellulose membrane (Cat#88018 Life technologies, Carlsbad, CA, USA). Blots were blocked in 5% ...
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bioRxiv - Bioengineering 2022Quote: ... and the equivalent of 10 μg of chlorophyll was loaded per lane on a Tris-Glycine based SDS-gel (Mini-Protean®TGX, Bio-Rad or Novex™ 8%, Thermo Fisher) and transferred onto a PVDF membrane using the Trans-Blot®Turbo™ Transfer System (Bio-Rad) ...
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bioRxiv - Cell Biology 2022Quote: ... protein lysate of 1 x 107 cells was loaded on a 4-12% tris-glycine gel (Novex™WedgeWell™, XP04122BOX, Thermo Fisher Scientific) and run in tris-glycine buffer (25 mM Tris ...
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bioRxiv - Genomics 2022Quote: ... The integrity and concentrations of the synthesized dsRNA were determined by 1.5% agarose/Tris-acetate-EDTA gel and Nanodrop (Thermo Scientific Nanodrop 2000, Waltham, MA). RNA interference was done by feeding newly emerged males 10 ng/μL dsRNAapoLp or 10 ng/μL dsRNAGFP dissolved in 30% honey water for 2 days ...
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bioRxiv - Genetics 2022Quote: ... The electrophoresed DNA is then transferred from the gel to a nitrocellulose membrane using a Owl HEP Series Semidry Electroblotting System (ThermoFisher Scientific, catalogue #HEP-1). The transferred membrane is then immersed in denaturing solution (1.6% w/v NaOH pellets in ddH2O ...
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bioRxiv - Microbiology 2022Quote: ... 2 mM EDTA pH 8) agarose gel for 75 m at 80V using GeneRuler 100 bp plus DNA ladder (Thermofisher Scientific, Waltham, MA, USA).
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bioRxiv - Microbiology 2022Quote: The protein concentrations within the fractionated sample loaded onto the gel was normalized by total protein content using the bicinchoninic acid (BCA) assay (Thermo Scientific Pierce, Waltham, MA). Proteins were separated by 12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) ...
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bioRxiv - Neuroscience 2022Quote: The samples were first diluted to concentrations ∼50 pM before being loaded into a 200 μL gel-loading tip (Life Technologies, Carlsbad, CA, USA) attached to the inlet port of a microfluidic channel (25 μm in height ...
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bioRxiv - Microbiology 2022Quote: ... Products were visualized on 1% agarose gels and purified using a MagJET NGS Cleanup and Size Selection Kit (Thermo Fisher Scientific, Waltham, MA, USA). The resulting purified libraries were finally quantified using a Quantus fluorometer (Promega ...
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bioRxiv - Molecular Biology 2024Quote: ... 20 μg of total protein lysate was resolved by electrophoresis on Bis-Tris 4-12% NuPAGE polyacrylamide gels using the Novex Mini Cell system (Invitrogen/Thermo Fisher Scientific, Waltham, MA). The proteins were then transferred to a PVDF membrane ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were harvested by scraping in cold PBS and prepared for 1-D gel analysis using Click-IT L-azidohomoalanine protein labeling reagents (Life Technologies C10102, C10276, B10185) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... The gel was prepared by adding 5 µL of the PCR probes and 2 µL of the ladder (Thermo Fisher Scientific, Waltham, MA, USA) and running the reaction at 90 V for 2 hours and 45 minutes ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The reactions were then loaded onto a 1% agarose gel and electrophoresed in an Owl™ D3-14 electrophoresis box (Thermo Scientific, MA, USA) containing 1X TBE buffer for 30 min at 180 V delivered from an Owl™ EC 300 XL power supply (Thermo Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were mixed with lithium dodecyl sulfate loading buffer and heated before fractionation on either NuPAGE™ Bis-Tris 8% Midi Gel (Invitrogen™, Cat. #WG1003BOX) or SurePAGE™ Bis-Tris 8% mini gel (GenScript ...
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bioRxiv - Immunology 2024Quote: ... PCR products were loaded on a 2% agarose gel in 1X TAE buffer containing 0.01% MilliporeSigma™ GelRed™ Nucleic Acid Stain (Fisher Scientific, catalog # SCT123) with electrophoresis performed at 200V for 30 minutes for the 3’ loxP sequence reaction and 40 minutes of the 5’ loxP sequence reaction ...
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bioRxiv - Immunology 2024Quote: ... Samples were then heated for 10 min at 95 °C and loaded onto a precast NuPAGE™ 4-12 % Bis-Tris polyacrylamide gel in NuPAGE™ MES SDS running buffer (Thermo Fisher Scientific). Electrophoresis was performed at 200 V for 45 min and gels were stained overnight with Quick Coomassie stain (Protein Ark) ...
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bioRxiv - Biophysics 2024Quote: ... Samples containing 20 µg of protein were separated on SDS-polyacrylamide gels and transferred to PVDF or NC membranes (Thermo Fisher, Waltham, MA, USA). Membranes were blocked in 5% bovine serum albumin in TBST for 1 hour before staining with primary antibodies overnight at 4°C ...
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bioRxiv - Bioengineering 2023Quote: ... in 2.5 μl NuPAGE® LDS Sample Buffer (4×) and 1 μl NuPAGE® Sample Reducing Agent (10×)] were loaded in Bolt 4-12% Bis-Tris Plus Gels (Thermo Fisher Scientific, USA). Samples were denatured at 95°C for 3 mins ...