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Citations for Thermo Fisher :
9151 - 9200 of 9427 citations for Vertical Gel Casting Cassettes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2021Quote: ... For all Coomassie stained gels proteins were separated by SDS-PAGE as above and then stained with SimplyBlue™ SafeStain (Thermo Fisher Scientific) as per manufacturer’s standard protocol.
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bioRxiv - Microbiology 2020Quote: ... The quality of extracted DNA was assessed by visualization on a 1% agarose gel with a high molecular mass ladder (Invitrogen, ThermoFisher Scientific, LLC, USA).
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bioRxiv - Pathology 2021Quote: Amplicons were purified by agarose gel electrophoresis and sequenced directly using the BigDye Terminator v3.1 Cycle Sequencing Kit on an ABI Prism R3500 (Applied Biosystems, Foster City, CA.). Twenty six previously published sequences ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Equal protein loads were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and proteins were transferred to nitrocellulose membranes (Thermo Scientific, Dreieich, Germany). The following primary antibodies were used at the indicated dilutions ...
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bioRxiv - Immunology 2020Quote: ... Lysate equivalent to 2×106 cells per well was run on a NuPAGE 4 to 12% Bis-Tris gel (Thermo Fisher Scientific, USA). Western blots were performed on samples and membranes were probed with anti-PTPN22 (clone D6D1H ...
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bioRxiv - Cell Biology 2019Quote: ... Gels were then functionalized overnight at 4°C with 250 μg/mL fibronectin using the photoactivatable crosslinker Sulfo-SANPAH (Thermo Fisher Scientific, 22589). After 24 hours CSE exposure ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were continuously recorded for 10 minutes while full gel relaxation was accomplished by a treatment with 0.05% trypsin-EDTA (Thermo Fisher Scientific, 25300-062).
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bioRxiv - Microbiology 2019Quote: ... 5 or 7 μL of the completed PCR product was then loaded onto 1.5% agarose (Vivantis, Selangor Darul Ehsan, Malaysia) gels prepared in 1X TAE buffer (Invitrogen, Grand Island, NY, USA) and 1:10,000 dilution of GelRed (Biotium ...
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bioRxiv - Microbiology 2019Quote: ... Samples were sonicated and heated for 5 min at 95°C before loading onto a Novex™ WedgeWell™ 4-20% Tris-Glycine Gel (ThermoFisher #XP04200BOX). Proteins were transferred to polyvinylidene difluoride (PVDF ...
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bioRxiv - Molecular Biology 2019Quote: ... were resolved by 4 – 20% Bis-Tris gels (GeneScript) and transferred to PVDF membranes using iBlot® 2 Dry Blotting System (Thermo Fisher Scientific). Blots were probed using previously described primary antibodies ...
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bioRxiv - Epidemiology 2019Quote: ... The amplified PCR products were recovered from agarose gels and cloned into the Zero Blunt TOPO vector (Thermo Fisher Scientific, Carlsbad, CA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2021Quote: ... and run on a NuPAGE™ 3-8% Tris-Acetate Protein Gel with NuPAGE™ Tris-Acetate SDS Running Buffer (ThermoFisher Scientific, LA0041) in a XCell SureLock™ Mini-Cell (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... The samples were then separated on 10% polyacrylamide gel alongside the dual-color Page Ruler Prestained Protein size markers (Thermo Fisher Scientific, Poland) in the Tris-Tricine SDS-PAGE system ...
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bioRxiv - Biochemistry 2021Quote: ... SDS-PAGE Bolt™ 4-12% Bis-Tris Plus Gel and PageRuler™ Unstained Protein Ladder molecular weight standard were bought from Thermo Fisher Scientific ...
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Disruption of the TCA cycle reveals an ATF4-dependent integration of redox and amino acid metabolismbioRxiv - Biochemistry 2021Quote: ... Samples were loaded onto 4-12% Bis-Tris Bolt™ gradient gels and run at 160V constant for 1h in Bolt™ MES 1X running buffer (Thermo Scientific). Dry transfer of the proteins onto a nitrocellulose membrane was done using iBLOT2 (Thermo Scientific ...
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bioRxiv - Microbiology 2021Quote: 5 - 10μg of protein from the cell lysates were run on 4-12% NuPAGE Bis-Tris SDS-PAGE gels (Invitrogen by ThermoFisher Scientific®) and transferred to PVDF membranes (ThermoFisher Scientific® ...
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bioRxiv - Cell Biology 2019Quote: ... then stained with sypro ruby red and a gel slice containing the entire fraction was cut for in-gel trypsin digestion and analysis on a LC-MS/MS apparatus (Thermo scientific Orbitrap Fusion) at the Quebec Genome Center.
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bioRxiv - Cell Biology 2021Quote: ... 10µg of protein was submitted to SDS-PAGE electrophoresis using NuPAGE Novex or Bolt 4-10% Bis-Tris gels (Thermo Fisher Scientific, USA). Proteins were transferred to nitrocellulose membranes using the iBlot Dry Blotting System (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... The denatured protein samples were separated by Novex™ WedgeWell™ 4-20% SDS-PAGE Tris-Glycine gel and transferred to PVDF membrane (iBlot™ 2 Transfer Stacks, Invitrogen) using iBlot 2 Dry Blotting System (Cat ...
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bioRxiv - Microbiology 2020Quote: ... SimplyBlue™ SafeStain and NuPAGE®Novex® 4-12% Bis-Tris Protein Gels (1.0 mm 10 well) were purchased from Life Technologies. Tris (hydroxymetthyl ...
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bioRxiv - Molecular Biology 2020Quote: ... Glycosylation was detected by in-gel periodic acid-Schiff staining using the Pro-Q Emerald 488 glycoprotein staining kit (Invitrogen, Carlsbad, CA, USA) and imaged using a Typhoon FLA 9000 laser bed scanner (GE Healthcare Bio-Sciences ...
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bioRxiv - Microbiology 2020Quote: ... recombinant proteins were purified by affinity chromatography as described previously (27) and loaded on NuPAGE 4-12% BisTris gels (Invitrogen, Carlsbad, CA, USA) to confirm the correct molecular weights and assess their purity.
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bioRxiv - Microbiology 2020Quote: Thirty ml of the wastewater sample were stained overnight with SYBR™ Green I nucleic acid gel stain (Molecular Probes, Life Technologies, USA). Sample was then processed by flow cytometry for sorting using the BD FACSAria™ Fusion cell sorter cytometer (BD Biosciences) ...
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bioRxiv - Microbiology 2020Quote: Thirty ml of the wastewater sample were stained overnight with SYBR™ Green I nucleic acid gel stain (Molecular Probes, Life Technologies, USA). Sample was then processed by flow cytometry for sorting using the BD FACSAria™ Fusion cell sorter cytometer (BD Biosciences) ...
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bioRxiv - Microbiology 2021Quote: ... PCR products were inspected on a 1.5% agarose gel and quantified using the Qubit dsDNA HS Kit (Qubit 2.0, Life Technologies, Foster City, CA, USA). PCR products were normalized to 240 ng each ...
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bioRxiv - Microbiology 2021Quote: ... No amplicon was visible in triplicate DNA control reactions when 4 μl were run on a 1.5% agarose gel visualized with SYBR Safe (ThermoFisher Scientific; cat. no. S33102). PCR products were plate purified (Millipore Multiscreen filter plates ...
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bioRxiv - Microbiology 2021Quote: ... 50 µg of each total lysate (mixture of murine and cryptococcal proteins) from the protein isolation step was run on a 12% NuPAGE™ tris-acetate gel (Thermo Fisher Scientific) two centimeters into the gel prior to Coomassie R250 staining and water de-staining for subsequent proteomic analysis.
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bioRxiv - Molecular Biology 2020Quote: ... The elute was run 2 cm into a 1.5 mm NuPAGE™ Novex™ 4-12% Bis-Tris protein gel (Thermo Fisher Scientific), stained with Coomassie blue and distained with distaining solution (10% acetic acid and 50% methanol) ...
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bioRxiv - Genomics 2021Quote: ... Qualitative and quantitative RNA assessment of all samples was done by gel electrophoresis (1% agarose), a NanoPhotometer® P330 (Implen, Munich/Germany) and a Qubit 2.0 (Invitrogen, Carlsbad, CA/USA).
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bioRxiv - Molecular Biology 2020Quote: ... Fragments migrating above the 5 kb band of the marker were extracted using the Silica Bead DNA gel extraction kit (Thermo Fisher Scientific, K0513) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... The purity of produced viruses was routinely checked by silver staining (Pierce, Germany) after gel electrophoresis (Novex™ 4–12% Tris–Glycine, Thermo Fisher Scientific) according to the manufacturer’s instruction ...
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bioRxiv - Immunology 2022Quote: ... and bands cut out for automated in-gel digestion and identification by Liquid Chromatography with tandem mass spectrometry (LC-MS/MS) using a Q Exactive Plus (Thermo Fisher Scientific, UK) at the University of Aberdeen proteomics facility.
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bioRxiv - Cell Biology 2022Quote: Blue native PAGE (BN-PAGE) was performed as outlined by the NativePAGE™ Novex® Bis-Tris Gel System manual (MAN0000557, Life Technologies). 3.7-9.25 ODs of cells were pelleted at 1,900 rcf for 2 minutes at 4°C ...
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bioRxiv - Biophysics 2022Quote: ... Afterwards the gel premix was prepared by mixing 500 μL Acrylamide stock solution with 250 μL 2% Bisacrylamide (BA, Fisher Scientific, BP1404-250). The premix was diluted in PBS to achieve the different shear moduli according to the table 1 ...
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bioRxiv - Cancer Biology 2022Quote: ... # 1536051 (Affi-Gel-15)) was aliquoted (12.5 µL pure resin per column) and resuspended using HPLC-grade distilled water (Fisher Scientific, Cat. # W5-4). Affi-Gel resin was divided equally into Pierce™ Spin Columns (Fisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... PCR products corresponding to the size of the amplified COI gene were retrieved from the gel and DNA sequencing was performed on both strands using a 3130xl Genetic Analyzer (Biosystems; Thermo Fisher Scientific, USA). The raw sequence data were edited and assembled using Bioedit software ...
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bioRxiv - Cell Biology 2022Quote: 20 μg of total protein normalized by Bradford assay was run in each well of a pre-cast Bis-Tris gel (ThermoFisher Scientific, Catalog #NP0336) in MOPS buffer (ThermoFisher Scientific ...
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bioRxiv - Developmental Biology 2022Quote: ... 5 µg of histone sample was loaded per lane on a BioRad ‘any Kd’ Precast gel (cat. #4569033) alongside 5 µl PageRuler Prestained Protein Ladder (Thermo Scientific cat. #26616), and run 200V for 28 minutes ...
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bioRxiv - Microbiology 2022Quote: Purified virions were heated at 95 °C for 10 min before separation on 4-12% Bis-Tris polyacrylamide gels (Thermo Fisher Scientific NW00125BOX) and transfer to nitrocellulose membranes ...
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bioRxiv - Microbiology 2022Quote: ... the resulting DNA fragments were separated by loading 10 µl of PCR product onto a 1.5% agarose gel stained with ethidium bromide (Invitrogen, Thermo Fisher Scientific, Waltham, USA) in a 1 × Tris/acetate EDTA (TAE ...
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bioRxiv - Plant Biology 2022Quote: ... the integrity of extracted RNA was checked in 2% agarose gel and also estimated spectrophotometrically with Multiskan Sky Microplate Spectrophotometer (Thermo Fisher Scientific, US). SuperScript® III First-Strand Synthesis System (Invitrogen ...
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bioRxiv - Plant Biology 2023Quote: ... We confirmed RNA integrity using 1.2% agarose gels and quantified the amount using a Nanodrop 2000c Spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA). We confirmed the absence of DNA via the lack of conventional PCR amplification of tomato and Botrytis tubulin and actin genes ...
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bioRxiv - Neuroscience 2022Quote: ... the purity and integrity of dsRNA were confirmed through a 1.5 % agarose gel and quantified using NanoDrop™ (Thermo Fisher Scientific, Massachusetts, US). The specificity of the dsRNA for each PPK was validated in silico using BLASTn searches against the R ...
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bioRxiv - Systems Biology 2024Quote: ... All PCR products were confirmed for unique and accurate amplification in agarose gels and purified using GeneJET PCR Purification Kit (Thermo Scientific, Waltham, MA). dsRNA was synthesized with 1 µg of purified PCR products using the T7 RiboMAX Express RNAi System ...
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bioRxiv - Plant Biology 2024Quote: The degradation and contamination of RNA were monitored on 1% agarose gels and the quality and integrity of RNA were assessed using a NanoDrop spectrophotometer (840-317400, Thermo Scientific, DE, USA). A total amount of 1.5 μg RNA per sample was used as input material for the RNA sample preparations ...
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bioRxiv - Microbiology 2024Quote: ... Quality control of amplified products was achieved by running a high-throughput Invitrogen 96-well-E-gel (Thermo Fisher Scientific, Waltham, MA, USA). PCR products from the same samples were pooled in one plate and normalised with the high-throughput Invitrogen SequalPrep 96-well Plate kit ...
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bioRxiv - Microbiology 2024Quote: Proteins were subjected to reduction in 4X reducing sample buffer (8% SDS, 400 mM Dithiothreitol) and resolved on a precast 4%–12% SDS-polyacrylamide gel (Thermo Fisher Scientific, #XP04125BOX). Coomassie staining was performed using Imperial Protein Stain (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2023Quote: ... RNA quality was assessed by electrophoresis in agarose gel and quantified using a QubitTM RNA Broad Range kit and Qubit 2.0 Fluorometer (ThermoFisher Scientific, Waltham, MA, USA).
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bioRxiv - Cell Biology 2023Quote: ... NuPAGE MOPS SDS running buffer was used to run the gels and Prestained PageRuler plus protein ladder 10-250 K (Thermo Scientific, Bremen, Germany) served to estimate protein size ...
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bioRxiv - Microbiology 2023Quote: ... and SDS-PAGE was performed with NuPAGE Novex 3 to 8% Tris-acetate gels in NuPAGE Tris-acetate SDS running buffer (Life Technologies Corp., USA) to resolve proteins >150kDa ...