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Citations for Thermo Fisher :
9351 - 9391 of 9391 citations for Mumps Virus Nucleoprotein Strain L Zagreb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... RT-qPCR was performed using 2 μL of 1:5 diluted cDNA in a 20 μL volume reaction with a PowerUp SYBR Green Master Mix (Thermo Fisher Scientific, Waltham, MA, USA), as described previously (30) ...
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bioRxiv - Neuroscience 2024Quote: ... antibodies overnight at 4°C and then incubated with Alexa Fluor 488-donkey anti-rabbit IgG (H + L) (1:200, Life Technologies, Gaithersburg, MD, USA, #A21208) for 1h ...
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bioRxiv - Physiology 2024Quote: Murine NIT-1 β cells (ATCC cat. CRL-2055) were maintained in 25mM high glucose DMEM supplemented with 2 mM L-glutamine (Gibco cat. 25-030-081),10% FBS (R&D cat ...
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bioRxiv - Neuroscience 2024Quote: ... We then washed and incubated slices for one hour with secondary antibodies diluted at a ratio of 1:200 (Alexa Fluor 488 goat anti-rabbit IgG (H+L) (Thermo Fisher Scientific Cat# A-11008); Alexa Fluor 594 goat anti-mouse IgG (H+L ...
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bioRxiv - Cancer Biology 2024Quote: ... with formulations calling for L-WRN conditioned medium instead using 500 pM Gibco™ Wnt Surrogate-Fc Fusion Recombinant Protein (Thermo Fisher Scientific, cat# PHG0402), 500 ng/mL recombinant RSPO1 (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... we transferred 5 μL of blood from the dorsal aorta on a 48-well plate with 150 μL of Avian KO-DMEM (Thermo Fisher Scientific, Waltham, MA, USA) supplemented with growth factors ...
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bioRxiv - Immunology 2024Quote: ... incubated at room temperature for 2 hours with Alexa Fluor® 647-conjugated donkey anti-mouse IgG (H+L) antibodies (1:500, Invitrogen, # A-31571, RRID: AB_162542) and 0.8 µM Hoechst 33342 at room temperature ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and 5 μL of the extract was subjected to LC-MS/MS analysis using API-3000 with an electrospray ion source (Applied Biosystems Instruments, Foster City, CA, USA) and an Agilent 1100 HPLC instrument (Agilent Technologies ...
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bioRxiv - Cancer Biology 2020Quote: ... cell line GRANTA-519 waskept in culture in RPMI 1640 medium supplemented with 10% heat-inactivated FBS 2 mM L-glutamine and 1% penicillin/streptomycin antibiotics (Gibco®, Thermo Fisher Scientific, Carlsbad, CA, USA). For cultivating the lymphoblastoid cell line (LCL ...
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bioRxiv - Genetics 2021Quote: ... slides were washed twice with 1 × PBS containing 0.05% (v/v) Tween-20 and incubated with goat anti-rabbit IgG (H+L) Alexa Flour 568 (Thermo Fisher Scientific, A-11011; 1:500 dilution) and goat anti-mouse IgG (H+L ...
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bioRxiv - Microbiology 2021Quote: ... Samples were injected in randomized order at a volume of 10 μL and eluted using a polar to apolar gradient formed using Ultrapure water (A) and LC/MS grade acetonitrile (Thermo Fisher Scientific, San José, CA, USA) (B) ...
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bioRxiv - Cell Biology 2020Quote: ... sections were washed 2 times for 8 minutes in DPBS containing 0.3% Triton X100 and then incubated for 1 hour in blocking solution containing the secondary antibody (1/200, Invitrogen P10994 Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Acquisitions were performed in 1,000-10,000 m/z range with a 4 s scan time in positive mode The mass spectrometer was also calibrated using singly charged ions produced by a 2 g/L solution of cesium iodide (Acros organics, Thermo Fisher Scientific, Waltham, MA USA) in 2-propanol/water (50/50 v/v) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The mass spectrometer was calibrated using singly charged ions produced by a 2 g/L solution of cesium iodide (Acros organics, Thermo Fisher Scientific, Waltham, MA USA) in 2-propanol/water (50/50 v/v) ...
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bioRxiv - Immunology 2020Quote: ... in basal media (DMEM/F12; 2mM Glutamax; 10mM HEPES; 1x Antibiotic-Antimycotic; 1x N2 supplement; 1x B27 supplement; all ThermoFisher, and 1mM Acetyl-L-cycteine, Sigma) supplemented with EGF (50ng/ml ...
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bioRxiv - Microbiology 2022Quote: ... the cells were incubated with the following secondary antibodies: Alexa Fluor™ 633 Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody (1:500; Thermo Fisher Scientific cat. No. A21052), Alexa Fluor™ 647 Donkey anti-Mouse IgG (H+L ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... dissociation of EBs was performed with collagenase II (200 U/L, Worthington, LS004176 in Hank’s balanced salt solution minus Ca2+/ Mg2+, Gibco, 14175-053 3.5 h, normoxia, 37 °C) (Breckwoldt et al. ...
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bioRxiv - Biochemistry 2024Quote: ... 10 μL of lipid sample was then aspirated and introduced via nano-ESI to an Orbitrap Fusion Lumos mass spectrometer (Thermo Fisher Scientific, San Jose, CA, USA) using an Advion Triversa Nanomate (Advion ...
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bioRxiv - Immunology 2023Quote: ... the sections were washed three times and incubated with the secondary antibodies (goat anti-rat IgG (H+L) Cross Adsorbed secondary antibody Alexa Fluor546 (Invitrogen, cat. no. A-11081, Karlsruhe, Germany)) for 1 hr at RT ...
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bioRxiv - Cell Biology 2023Quote: ... in 2% BSA in PBS for 5 min and in Reaction Cocktail solution (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 25 mM Cooper reactive, Alexa FluorTM 488 Azide ThermoFisher A10266, and 62.38 mM L-Ascorbic Acid) for 30 minutes ...
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bioRxiv - Developmental Biology 2023Quote: ... the embryos were washed three times in PBST and then incubated with Alexa Fluor 555 highly cross-adsorbed conjugated donkey anti-rabbit IgG (H+L) secondary antibody (Thermo Fisher Scientific, Cat. No. A-31572), Alexa Fluor 488 conjugated donkey anti-rat IgG (H+L ...
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bioRxiv - Bioengineering 2023Quote: ... cells were washed three times at 5 minutes each with DPBS before incubation for 1 hour at room temperature in the dark with secondary antibodies in blocking buffer (goat anti-rabbit IgG (H + L) Cross-Adsorbed Secondary Antibody conjugated to Alexa Fluor 488 (ThermoFisher, 1:200 dilution, Cat # A-11008) or goat anti-mouse IgG1 Cross-Adsorbed Secondary Antibody conjugated to Alexa Fluor 488 (ThermoFisher ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were then washed three times with PBS for 15 min and incubated with anti-rabbit IgG (H+L) highly cross-adsorbed Alexa Fluor 594 secondary antibody (Thermo Fisher Scientific, A-21207, 1:2000) for 1 hr in the dark and at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... sections were washed two times for 8 min in DPBS containing 0.3% Triton X100 and then incubated for 1 hr in blocking solution containing the secondary antibody (1/200, Invitrogen P10994 Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody ...
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bioRxiv - Systems Biology 2023Quote: ... Antibodies were detected using the following secondary antibodies for their appropriate hots: Goat anti-Rabbit IgG (H+L) Alexa Fluor™ 488 (Thermo Fisher Scientific Cat. No. A11034), and Donkey anti-Mouse IgG (H+L ...
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bioRxiv - Physiology 2024Quote: ... tissues/organs were washed with DPBS four times on a rocker over the course of an hour and subsequently incubated overnight at 4°C with a goat anti-rabbit Alexa Fluor® 568 IgG (H+L) secondary antibody (Molecular Probes, Life Technologies, Eugene, OR, USA) diluted 1:200 in 10% NSS made up in DPBS and protected from light ...
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bioRxiv - Molecular Biology 2024Quote: ... and the middle part of the muscle were cryo-sectioned following embedding into OCT compound and stained with primary antibodies for SMA (fluorophore-conjugated) and for CD31 followed by fluorophore-conjugated secondary antibody (Invitrogen, donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody ...
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bioRxiv - Molecular Biology 2024Quote: ... in HepG2 medium (Advanced MEM minus L-Glutamine [Gibco] + 10% [v/v] HI-FBS [Gibco] + 1% [v/v] penicillin-streptomycin + 2 mM Glutamax [Gibco] and 0.1% [v/v] amphotericin B). Once the HepG2 or HC-04 cells were grown to the desired concentration ...
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bioRxiv - Genomics 2021Quote: ... Two hundred microliters (200 μL) of the pharyngeal washes were first processed for RNA extraction in the Thermo Scientific™ KingFisher™ Flex Purification System (Thermo Fisher Scientific, Waltham, MA USA), using the IndiMag® Pathogen Kit (QIAGEN® GmbH ...
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bioRxiv - Immunology 2022Quote: ... tissues were minced with a razor blade and subjected to a 30-minute enzymatic digestion at 37 °C in 3 mL of 1:1 DMEM/F12 media (Gibco, Grand Island, NY; 200 mM L-glutamine) with 1X-collagenase/hyaluronidase (10X stock ...
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bioRxiv - Microbiology 2020Quote: ... cells were washed multiple times and incubated with the secondary antibody Alexa Fluor 546 goat anti-rabbit IgG (H+L) (Invitrogen; 1:200 dilution in PBS-BSA-Saponin) in the presence of 1 μM of SiR-DNA for 2 hours in the dark ...
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bioRxiv - Immunology 2020Quote: OT-II 10-day WT or CCR5−/− lymphoblasts were plated in poly-L-lysine-coated coverslips (Nunc Lab-Tek Chamber Slide, Thermo Scientific; 50 μg/ml, overnight, 4°C). After adhesion (1 h ...
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bioRxiv - Cell Biology 2022Quote: ... was used as a primary antibody and a goat anti-mouse IgG (H+L) cross-adsorbed antibody Alexa Fluor™ 647 (Thermo Fisher Scientific, A-21235; 1:500) was used as a secondary antibody ...
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bioRxiv - Cell Biology 2022Quote: ... was used together with transferrin-Alexa Fluor 594 or with donkey anti-goat IgG (H+L) secondary antibody-Alexa 594 (Thermo Fisher, cat. no. A-11058, 1:500).
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bioRxiv - Cancer Biology 2024Quote: ... antibodies were incubated overnight with rocking at 4°C and secondary antibodies (goat anti-rabbit [cat# 31462, Invitrogen, 1:10,000] or goat anti-mouse [cat#31432, Invitrogen, 1:10,000] IgG H&L cross absorbed HRP) were incubated for 2 hours at room temperature ...
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bioRxiv - Biophysics 2023Quote: ... Sections were then washed with PBS for 2×30 minutes and incubated with Abberior STAR580 goat anti-rabbit IgG (Abberior GmbH, Göttingen, Germany) (1:250 dilution) and AlexaFluor-488-conjugated phalloidin (Invitrogen A-12379, 66 μmol/L, 1:500 dilution). The sections were then washed with PBS for 2×15 minutes and covered with number 1.5H high precision coverslips (Marienfeld Superior ...
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bioRxiv - Physiology 2024Quote: ... tissues/organs were washed with DPBS four times on a rocker over the course of an hour and subsequently incubated overnight at 4°C with a goat anti-rabbit Alexa Fluor® 568 IgG (H+L) secondary antibody (Molecular Probes, Life Technologies, Eugene, OR, USA) diluted 1:200 in 10% NSS made up in DPBS and protected from light ...
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bioRxiv - Microbiology 2024Quote: Iron coupons were gently rinsed with phosphate buffer (pH 7.4) and stained with Live/Dead BacLight™ bacterial staining kit (Molecular Probes L-7012, Thermo Fisher Scientific, USA) for 10 min in the dark ...
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Tuning trophoblast invasion in a gelatin hydrogel via soluble cues from the maternal-fetal interfacebioRxiv - Bioengineering 2020Quote: ... 5×5 minute washes in 0.1% Tween 20 solution in PBS were performed and samples were incubated in secondary antibody solution (Thermo Fisher A-21428 Goat Anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody AlexaFluor 555 ...
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bioRxiv - Microbiology 2024Quote: For biofilm visualization with confocal scanning laser microscopy the Fe0 coupons were washed with PBS and then stained with the Live/Dead BacLight™ bacterial staining kit (Molecular Probes L-7012, Thermo Fisher Scientific, USA) for 10 min in the dark ...
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bioRxiv - Microbiology 2024Quote: ... Cells were washed 3 times with PBS and stained with Alexa Fluor 647 conjugated Goat anti-Mouse IgG (H+L) (Thermo Fisher Scientific, Cat. no. A-21236, 1:1000) and Alexa fluor 488-conjugated Goat anti-Rabbit (Thermo Fisher Scientific ...