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Citations for Thermo Fisher :
8901 - 8950 of 9391 citations for Mumps Virus Nucleoprotein Strain L Zagreb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... M and L with three independent plants for each of the 1205A and 1205B lines using the TRIzol kit (Invitrogen, Carlsbad, CA) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... SCD ko pools and clones were cultured in DMEM/F-12 with 10% FBS and 0.8 g/L AlbuMAX II lipid-rich BSA (Thermo Fisher Scientific, # 11021029). After infection ...
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bioRxiv - Bioengineering 2023Quote: ... PCs were seeded into 6-well plates coated with collagen IV (100 μg/mL) and were cultured in DMEM (1 g/L glucose, Gibco, cat# 11885084) supplemented with 10% FBS (Sigma-Aldrich ...
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bioRxiv - Microbiology 2024Quote: ... The cells were washed 3 times with 1X PBS and incubated with goat anti-mouse IgG (H&L)-Alexa Fluor 488 (1:1000 dilution, Thermo Fisher Scientific) at 37°C for 2h ...
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bioRxiv - Immunology 2023Quote: ... and MC38-Ova (obtained from AstraZeneca) murine colon adenocarcinoma cells were cultured in DMEM and supplemented with 2mM L-glutamine (Thermo Fisher Scientific), 10% FBS (F9665 ...
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bioRxiv - Developmental Biology 2023Quote: ... goat anti-rat IgG (H+L) cross-adsorbed secondary antibody conjugated with Alexa Fluor 488 and 647 (A-11006 and A-21247, respectively; Thermo Fisher Scientific), and goat anti-mouse IgG (H+L ...
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bioRxiv - Cell Biology 2024Quote: ... 8 µl (25%) sample was taken as input and the rest of the samples incubated with 50 µl amylose resin (Thermo Fisher Scientific) which had been washed in buffer before incubating for 90 minutes at 4ºC in a rotating mixer ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 5 mM DTT, 50 mM HEPES, 3 mM MgCl2, 2 mM PMSF, 1 Pierce™ protease inhibitor mini tablet/2 L; Thermo Scientific). The lysate was sonicated then incubated with 500 units/1 L culture Benzonase Nuclease (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2024Quote: ... Ovaries were carefully removed from three female zebrafish and placed in a 100-mm Petri dish containing 60% Leibovitz L-15 medium (Invitrogen, Waltham, MA). Fat tissues and ligaments surrounding the ovaries were stripped off using a BD Microlance 26G needle (BD ...
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bioRxiv - Developmental Biology 2024Quote: Protein synthesis was assessed using the Click-iT® L-homopropargylglycine (HPG) Alexa Fluor 488 Protein Synthesis Assay Kit (Molecular Probes, USA), which measures newly synthesized proteins through quantification of the fluorescent HPG signal ...
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bioRxiv - Developmental Biology 2024Quote: ... Individual follicles were obtained by breaking down the ovaries into small pieces with a 26 G needle and incubating them in Dissection Media containing L-15 (Thermo Scientific, #11415064) with 1% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2024Quote: ... Fragments were end-repaired with Enzymatics low concentration end repair mix (Y9140-LC-L) and A-tailed with Recombinant Taq (Life Technologies, 100021276). Y-Adapters were pre-annealed by incubating a MiSeq Common Adapter with Adapters (A01-A10 ...
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bioRxiv - Immunology 2024Quote: ... 1:400 and for 1 hour at room temperature with the quaternary antibody: F(ab)’2-goat anti-rabbit IgG (H+L) A488 (Invitrogen, A-11070) 1:1000 ...
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bioRxiv - Microbiology 2024Quote: ... probed with anti-GFPmut2 primary antibody (1:1000 dilution, rabbit pAb, custom Pocono RF&L) with anti-rabbit conjugated to HRP (1:1000 dilution, Invitrogen, Cat #A16104) or streptavidin-HRP (1:1000 dilution ...
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bioRxiv - Biochemistry 2024Quote: The samples were analyzed by injecting 5 μl of the extracted solutions into a Dionex Ultimate 3000 ultra-HPLC system connected to a photodiode array detector (Thermo Fisher Scientific), with a reverse-phase column (ZORBAX Eclipse Plus C18 ...
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bioRxiv - Biophysics 2024Quote: Cells were cultured in either RPMI with L-glutamine (FisherScientific, cat# 12004997)—for suspension cells—or DMEM with high glucose (ThermoFisher, cat# 11965092)—for adherent cells—supplemented with 10% Fetal Bovine Serum (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2024Quote: ... and 800 μL of a viral solution comprising concentrated lentiviral particles and 8 μg/mL polybrene was added to the cells (Thermo Fisher Scientific). Cells were then spinfected at 1048 × g for 90 min at 30 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... PtenpeΔ/Δ and PtenpeΔ/Δ;L-gp130peKI/KI mice was done as previously described80 and magnetic cell separation (MagniSort technology, Thermo Fisher) was performed for EpCAM positive fraction using anti-mouse CD326 (EpCAM ...
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bioRxiv - Cell Biology 2024Quote: ... Each siRNA construct was transfected at 220 pmol/l precomplexed and incubated in the transfection reagent in Opti-MEM (Gibco, ThermoFisher Scientific) for 4 h ...
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bioRxiv - Biophysics 2024Quote: ... Fungal conidia were cultivated at 30°C for one week on the agar media prepared with 65 g/L YPD (Catalogue # DF0428175, Thermo Fisher Scientific) and pre-autoclaved ...
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bioRxiv - Neuroscience 2024Quote: ... The cDNA products were diluted 1:1 and 2 µL was analyzed by qPCR using custom primer sets and PowerUp SYBR Green Master Mix (10 µL total reaction, Applied Biosystems # A25742). RT-qPCRs were performed on a Quantstudio3 from Applied Biosystems ...
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bioRxiv - Neuroscience 2023Quote: ... was expressed in Expi293 cell line (3X106 cells/ml, 200 ml in 1 L flask) using ExpiFectamine™ 293 Transfection Kit (Gibco, A14524). After 48 hours ...
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bioRxiv - Cancer Biology 2023Quote: ... in serum-free culture medium (Advanced DMEM/F-12 supplemented with 0.6% glucose, 2 mM L-glutamine (ThermoFisher Scientific, Waltham, MA, USA), 5 mM HEPES ...
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bioRxiv - Biochemistry 2024Quote: HEK293T cells used for transient transfection for thermostabilization experiments were cultured in DMEM + GlutaMAX™-I with 4.5 g/L D-Glucose and Pyruvate (Gibco 31966-021), supplemented with 10 % (v/v ...
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bioRxiv - Microbiology 2023Quote: ... and pC-L (1 μg) that supply the trans-acting factors LCMV NP and L 70 using 10 μL of Lipofectamine 2000 (Thermo Fisher Scientific) and incubated at 37°C and 5% CO2 ...
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bioRxiv - Cell Biology 2023Quote: ... NEFL-/- 293T cells expressing NF-L-myc-6xHis were starved for 48 hrs of glucose: glucose-free DMEM (Thermo Fisher Scientific, 11966025) with 1 mM sodium pyruvate (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... From day 20 on cortical neurons were cultured on polyornithine (PO)/ laminin (L)/fibronectin (FN) coated wells and kept in neuronal differentiation media (Neurobasal media (#21103-049, Thermo Fisher Scientific), 1% Pen/Strep (#15140-122 ...
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bioRxiv - Neuroscience 2022Quote: ... Membranes were then washed and probed with horseradish-peroxidase-conjugated donkey anti-mouse or anti-rabbit IgG (H+L) cross-adsorbed secondary antibody (SA1-100, Invitrogen; 31438, Invitrogen) for 1h at RT ...
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bioRxiv - Cell Biology 2023Quote: The following secondary antibodies were used for IF: Donkey anti-goat IgG (H+L) Alexa Fluor 488 (1:200; ThermoFisher; A-11055); Goat anti-mouse Atto647N (1:200 Sigma) ...
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bioRxiv - Molecular Biology 2023Quote: ... were obtained from the IGBMC cell culture facility and cultured in DMEM (4.5 g/L glucose) supplemented with 10% fetal calf serum (Dutscher, S1810) and 100 U/mL penicillin 100 μg/mL streptomycin (Invitrogen, 15140-130). E14 mouse embryonic stem cells (mESCs ...
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bioRxiv - Molecular Biology 2023Quote: The resulting peptides (3 μL) were analysed by tandem mass spectrometry using a Q Exactive mass spectrometer equipped with an Easy nLC1000 system (Thermo Fisher Scientific). The peptides were separated using emitter columns (15 cm length x 100 μm ID x 360 μm OD x 15 μm orifice tip ...
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bioRxiv - Immunology 2023Quote: ... 2 μL of diluted cDNA (1:50) was used as template for qPCR reaction using PowerUp SYBR Green Master Mix (Applied Biosystems, A25742). For internal control ...
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bioRxiv - Microbiology 2023Quote: ... We used A549 growth media for seeding cells for viral passages and A549 viral media (DMEM, high glucose, with L-glutamine, without Na pyruvate (Invitrogen 11965-092), with 1% Pen+Strep ...
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bioRxiv - Cell Biology 2023Quote: ... Images were collected using a Talos L 120C TEM microscope at 80 kV with Velox software and a 4k × 4K Ceta CMOS Camera (Thermo Fisher Scientific).
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bioRxiv - Immunology 2023Quote: ... 20 µL) and analysed using an Orbitrap Fusion Lumos Tribrid Mass Spectrometer connected to a nano-LC Easy nLC 1200 (ThermoFisher Scientific, Singapore). The peptides were separated at a flow rate of 300 nL/min on C18 pre-column (Acclaim PepMap™ 100 ...
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bioRxiv - Immunology 2023Quote: ... followed by staining with 1:500 goat anti-rabbit IgG (H+L) secondary antibody tagged with Alexa Fluor 647 (Invitrogen, A-21244) + 1:50 SiglecF-Alexa Fluor 488 (BD ...
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bioRxiv - Immunology 2023Quote: ... and B16F10-Ova (obtained from AstraZeneca) murine melanoma cells were cultured in RPMI supplemented with 2mM L-glutamine (#21875034 Thermo Fisher Scientific), 10% FBS (#F9665 Sigma-Aldrich) ...
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bioRxiv - Plant Biology 2023Quote: ... Samples were resuspended in 600 µL of 0.1% TFA and then split into two aliquots such that two high pH fractionation columns (Pierce/ThermoFisher, Pistcataway, NY, USA) were loaded at maximum capacity (100 µg protein) ...
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bioRxiv - Cancer Biology 2023Quote: Cell viability was tested using the ”LIVE/DEAD Viability/Cytotoxicity Kit for mammalian cells” that contained Ethidium Homodimer-1 and Calcein-AM stain (ThermoFisher ; L-3224) dyes ...
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bioRxiv - Developmental Biology 2023Quote: ... E14 and E16 chick embryos in chilled L-15 mediumand subsequently incubated in 1 mM solution of 2-NBDG (N13195, Thermo Fisher Scientific) in L-15 medium at room temperature for 1 hour ...
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bioRxiv - Cancer Biology 2023Quote: ... Splenocytes were activated by seeding at a density of 2×106 cells in T cell media (advanced DMEM supplemented with 10% FBS, 2mM L-glutamine (Gibco, NY, USA), 1% PS ...
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bioRxiv - Bioengineering 2023Quote: CHO-suspension cells were plated at a concentration of 7.5x10^4 cells/mL in Freestyle CHO Expression medium supplemented with 8 mM L-glutamine (Thermo Fisher Scientific, MA) in 125-mL disposable polycarbonate Erlenmeyer flasks with vented caps (Fisher Scientific ...
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bioRxiv - Genetics 2023Quote: ... Secondary antibodies used were goat anti-mouse or goat anti-rabbit polyclonal IgG (H+L) HRP conjugates (ThermoFisher Scientific; #31430 or #32460) (1:5000 - 10,000).
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bioRxiv - Molecular Biology 2023Quote: ... for 1 h at room temperature, followed by two washes with complete culture medium (60% L-15 in ddH2O, supplemented with 1% Antibiotic-Antimycotic (Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2023Quote: ... Coverslips were rinsed 3 times with 1X PBS for 15 min and incubated with secondary antibodies goat anti-chicken IgY (1:2K, H+L, Alexa Fluor 647, Invitrogen, Cat# A21449) and goat anti-rabbit IgG (1:2K ...
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bioRxiv - Molecular Biology 2023Quote: ... D-001810-10-05) or Zmiz1 siRNA (Horizon, L-007034-00-0005) for 48 hours hours using Lipofectamine 3000 (Thermo Fisher, L3000015). The final concentration of siRNA solution was 200 nM ...
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bioRxiv - Immunology 2023Quote: ... the cells were washed twice with PBS + 1% BSA and stained with 10 μg/mL Alexa Fluor™ 647 Goat anti-Human IgG (H+L) Cross-Adsorbed Secondary Antibody (ThermoFisher Scientific) diluted in PBS with 1% BSA ...
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bioRxiv - Cell Biology 2023Quote: ... slides were washed in PBS and incubated with goat anti-rabbit immunoglobulin G (IgG) (H + L) secondary antibody conjugated with Alexa Fluor 594 (Invitrogen, 1:200). Slides from eWAT were incubated with anti-F4/80 (ab300421 ...
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bioRxiv - Biochemistry 2022Quote: ... secondary antibodies were diluted in blocking buffer as follows: Alexa Fluor 488-Goat anti-Mouse IgG (H+L) secondary antibody (1:1000; Life Technologies; A11029) an Alexa Fluor 568-Goat anti-Rabbit IgG (H+L ...
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bioRxiv - Biochemistry 2022Quote: ... 20 μl) and analysed using an Orbitrap Fusion Lumos Tribrid Mass Spectrometer connected to a nano-LC Easy nLC 1200 (ThermoFisher Scientific, Singapore). The peptides were separated at a flow rate of 300 nL/minute on C18 pre-column (Acclaim PepMap™ 100 ...