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Citations for Thermo Fisher :
9051 - 9100 of 9391 citations for Mumps Virus Nucleoprotein Strain L Zagreb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... and a 300μL aliquot was saved and mixed with buffer (100mM/L Tris-HC1 pH 7.6, 4% SDS, protease inhibitor cocktail EDTA-free 100x Thermo Fisher Scientific, Loughborough, UK) to prepare the crude homogenate ...
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bioRxiv - Cell Biology 2021Quote: ... albicans was prepared as described previously and added to each well at a multiplicity of infection of five in 100 μl of complete RPMI (RPMI 1640 with 2 mM L-glutamine, 10% heat-inactivated fetal bovine serum, and 1% penicillin-streptomycin; ThermoFisher Scientific, Waltham, MA). The plate was incubated at 37°C and 5% CO2 for two hours to allow mammalian cell/fungal interaction ...
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SARS-CoV-2 comprehensive receptor profiling: mechanistic insight to drive new therapeutic strategiesbioRxiv - Cell Biology 2021Quote: ... each pre-incubated at a 2:1 molar ratio with AlexaFluor647 donkey anti-sheep IgG (H+L) (Thermo Fisher, UK, catalogue # A-21448); or 5 μg/mL SARS-CoV-2 S test protein (Peak Proteins ...
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bioRxiv - Neuroscience 2020Quote: PBMCs were transfected with Epi5™ EBNA-1/OriP-based episomal vectors containing five pluripotent factors (Oct4, Sox2, Klf4, L-myc and Lin28) using the Neon® Transfection System (Invitrogen™, A15960) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... The irradiation effect on tendon cells was detected with a live/dead cell viability assay kit (Cat # L-7013, Life Technologies, Carlsbad, CA) performed according to the manufacturer’s protocols ...
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bioRxiv - Neuroscience 2022Quote: ... the secondary antibody used in staining was donkey anti-goat IgG (H+L) highly cross-adsorbed secondary antibody conjugated to Alexa Fluor Plus 488 (ThermoFisher Scientific, A11055;1:500). The RNAscope probes used in our experiments were ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... the culture medium was replaced with RPMI□+ □B27+insulin medium (RPMI 1640, Thermo Fisher; 2 mM L-glutamine, Thermo Fisher; 1×□B27 supplement with insulin, Thermo Fisher) supplemented with 2.5 µM IWP4 (Stemgent ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... We replaced the AK02N medium with RPMI□+□B27 medium (RPMI 1640, Thermo Fisher; 2 mM L-glutamine, Thermo Fisher; 1×□B27 supplement without insulin, Thermo Fisher) supplemented with 100 ng/mL Activin A (R&D ...
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bioRxiv - Molecular Biology 2022Quote: ... the Cas12a RNP complex was then mixed with 1 × 105 cells in Neon R buffer at the desired dosage and electroporated using Neon® Transfection System 10 L Kit (Thermo Fisher Scientific) using the suggested electroporation parameters ...
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bioRxiv - Molecular Biology 2022Quote: ... the Cas12a RNP complex was then mixed with 1 × 105 cells in Neon R buffer at the desired dosage and electroporated using Neon® Transfection System 10 L Kit (Thermo Fisher Scientific) using the suggested electroporation parameters ...
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Construction of a constitutively active type III secretion system for heterologous protein secretionbioRxiv - Synthetic Biology 2022Quote: ... Membranes were subsequently probed with goat anti-rabbit IgG (H+L) conjugated to HRP according to manufacturer’s instructions (Thermo Fisher, 1:1,000 dilution). Bands were detected with SuperSignal West Pico Plus substrate (Thermo Fisher ...
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bioRxiv - Biochemistry 2022Quote: ... The membrane was subsequently incubated with the goat anti-rabbit IgG (H+L) highly cross-adsorbed secondary antibody conjugated to the fluorophore Alexa Fluor plus 555 (diluted 1:5000, Thermo Fisher Scientific, USA) before imaging ...
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bioRxiv - Cell Biology 2022Quote: ... originally obtained from ATCC) were cultured at 37 °C and 5% CO2 in DMEM (high glucose 4.5 g/l; Gibco, Thermo Fisher cat# 10566024) supplemented with 10% FBS (Gibco ...
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bioRxiv - Microbiology 2022Quote: ... and goat anti-mouse IgG (H+L) highly cross-adsorbed secondary antibody (Alexa Fluor® 488; 1:500 dilution (Invitrogen, cat# A-11029)) ...
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bioRxiv - Genomics 2022Quote: ... HEK293FT cells were maintained at 37°C and 5% CO2 in D10 medium (DMEM with high glucose and stabilized L-glutamine (Caisson, no. DML23) supplemented with 10% fetal bovine serum (FBS; Thermo Fisher, no. 16000044)) ...
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bioRxiv - Microbiology 2022Quote: ... Cells were then washed and incubated with 1 µg/ml AlexaFluor647 Donkey Anti-Mouse IgG (H+L) (Thermo Fisher Scientific Corporation, OR, USA) at room temperature for 45 min ...
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bioRxiv - Cell Biology 2022Quote: ... Secondary antibodies used were goat anti-mouse or goat anti-rabbit polyclonal IgG (H+L) HRP conjugates (Thermo Fisher Scientific; #31430 or #32460).
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bioRxiv - Neuroscience 2020Quote: ... and cultured for 7 days in vitro in Neurobasal® medium supplemented with 2% v/v B-27® supplement medium and 1mM L-glutamine (all from Life Technologies) at 37 °C ...
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bioRxiv - Microbiology 2021Quote: ... BMD plates were made by preparing serial 2-fold dilutions of antimicrobial agents at twice the desired final concentration in 100 μL RPMI 1640 media with L-glutamine (Cytiva, Marlborough, MA) prepared with MOPS buffer (Fisher Scientific, Waltham, MA) in clear ...
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bioRxiv - Plant Biology 2021Quote: ... The samples were washed with PBS buffer three times and incubated in secondary antibody solution [1:1000 Alexa Fluor™ 546 goat anti-mouse IgG (H+L) (Thermo Fisher Scientific) for use in experiments with anti-HA and anti-catalase antibodies as the primary antibodies and 1:1000 Alexa Fluor™ 488 goat anti-mouse IgG (H+L ...
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bioRxiv - Plant Biology 2021Quote: ... for use in experiments with anti-HA and anti-catalase antibodies as the primary antibodies and 1:1000 Alexa Fluor™ 488 goat anti-mouse IgG (H+L) (Thermo Fisher Scientific) for use in experiments with the anti-α-tubulin antibody as the primary antibody ...
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bioRxiv - Microbiology 2020Quote: ... skin homogenate or 100 µl of insect homogenate was extracted in a 96-well plate with the MagMAX™ CORE Nucleic Acid Purification Kit (Applied Biosystems, A32700) using protocol A in a KingFisher™ Flex Magnetic Particle Processor (Applied Biosystems ...
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bioRxiv - Microbiology 2020Quote: Vero cells were seeded at 1.2 × 104 cells per well in Opti-PRO™ SFM supplemented with 4 mM L-glutamine and 1X Antibiotic-Antimycotic solution (Gibco/Thermo Fisher Scientific) in black ...
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bioRxiv - Microbiology 2021Quote: ... Secondary antibody (200 μL) was added (1:1,000 dilution of Alexa Fluor™ goat anti-mouse 594 IgG (H+L) (Invitrogen, A11005, Lot 1937185) in 1x PBS ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: The mouse eyes harvested after L-CNV induction at different days along with the eyes from untouched control mice were fixed in 4% PFA (Thermo Fisher Scientific, #43368) for 16 hours at 4°C ...
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bioRxiv - Systems Biology 2022Quote: ... medium completed with 10% FCS and 40 μM in both experimentg/mL gentamicin, diluted every 3rd/4th day 1/5. U2OS cells (ATCC, ref. HTB-96) were grown in DMEM (Gibco, 1g/l glucose) completed with 10% FCS and 40 μM in both experimentg/mL gentamicin ...
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bioRxiv - Cell Biology 2022Quote: ... MDA-MB-231 and MDA-MB-468 cells were cultured in Leibovitz’s L-15 medium (41300-039; Thermo Fisher Scientific,Waltham, MA, USA) supplemented with 10% FBS without CO2 supplementation.
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bioRxiv - Biophysics 2022Quote: ... We revealed the antigens coating with a Donkey anti-Goat IgG (H+L) Cross-Adsorbed Secondary Antibody (Alexa Fluor 647, Invitrogen, ref. A-21447) and analyzed the fluorescence intensity ...
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bioRxiv - Developmental Biology 2022Quote: ... The secondary antibodies used for immunofluorescent staining were Alexa Fluor 488/594-conjugated goat anti-rabbit IgG (H+L) (A-11008/A-11012, Invitrogen, Carlsbad, CA, USA) and goat anti-mouse IgG H&L (Alexa Fluor 488/594 ...
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bioRxiv - Microbiology 2020Quote: ... The peptide samples were loaded (2 µl) onto a C18-reversed phase Acclaim PepMap 100 trap column (2 cm x 75 µm, 3 µm particles; Thermo Fisher Scientific, USA) with 30 µL of buffer A (2% v/v acetonitrile ...
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bioRxiv - Microbiology 2019Quote: ... and then stained with anti-LCMV-WE recombinant NP immunized rabbit serum and HRP-goat anti-rabbit IgG (H+L) DS Grd (Life Technologies, Carlsbad, CA) (41) ...
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bioRxiv - Immunology 2019Quote: ... For each experiment frozen PBMCs were washed and rested overnight in RPMI 1640 pre-supplemented with L-glutamine (Life Technologies Corporation, Carlsbad, USA), 10% fetal calf serum (Gibco ...
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bioRxiv - Cell Biology 2020Quote: ... the cells were incubated for 1 h with an F(ab’)2-Goat anti-Mouse IgG (H+L) cross-adsorbed secondary antibody conjugated with Alexa Fluor 488 (Invitrogen, Thermo Fisher Scientific) diluted in blocking buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... eluted in 10 μl water and its quality was verified by ds-DNA concentration measurement with Qubit ds-DNA High Sensitivity assay (Thermo Fisher Scientific, USA) and BioAnalyzer (Agilent 2100 ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were washed Bond Wash solution for 3 × 3 minutes and then incubated with a goat anti-mouse IgG (H+L) AlexaFluor 488-conjugated secondary antibody (A-1101, ThermoFisher Scientific, Waltham, MA) 1:500 in PBS containing 3% goat serum ...
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bioRxiv - Neuroscience 2020Quote: ... Slides were washed with Bond Wash solution 3× 3 minutes then incubated with a goat anti-rabbit IgG (H+L) AlexaFluor 633-conjugated secondary antibody (A-21070, ThermoFisher Scientific, Waltham, MA) 1:500 in PBS containing 3% goat serum ...
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bioRxiv - Neuroscience 2020Quote: ... Dissociated cells were grown in neurobasal medium supplemented with 5 ml of L-glutamine (200 mM) and B27 supplement (Life Technologies, A3582801, Germany) with an exchange of 50% medium after 6 days in culture.
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bioRxiv - Biochemistry 2020Quote: ... and then incubated for 1 h in the blocking solution containing 0.1 % Tween-20 and AlexaFluor® 680 goat anti-rabbit (H+L) secondary antibody (1: 20, 000; ThermoFisher Scientific, Waltham, Massachusetts) (Table S5) ...
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bioRxiv - Molecular Biology 2021Quote: ... containing varying concentrations of the corresponding crowding agent: 0/5/10/305515% w/v of serine (L-Serine, 99%, ACROS Organics™, AC132660250), 0/5/10/15% w/v of Polyethylene glycol 3350 (PEG ...
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bioRxiv - Genetics 2019Quote: ... primary antibodies in blocking buffer containing 5% milk followed by the secondary antibodies of goat anti-mouse IgG (H+L) HRP (Life Technologies, 1:10000) or goat anti-rabbit IgG (H+L ...
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bioRxiv - Neuroscience 2020Quote: ... the digested neurovascular pellet was re-suspended in 1 mL of Mouse Brain Endothelial Cell Culture Medium (DMEM 1 g/L glucose, Mg+, Ca2+ (Gibco, Waltham, MA, USA), FBS 20 % (Gibco ...
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bioRxiv - Immunology 2021Quote: ... bone marrow was collected from femurs and tibia of BALB/c mice and then seeded into bacteriological Petri dishes at 4 × 106 in 10 ml of complete RPMI 1640 medium supplemented with 2mM L-glutamine (Life Technologies, Gaithersburg, MD), 100U/ml penicillin and 100μg/ml streptomycin ...
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bioRxiv - Immunology 2021Quote: ... cells were then washed and incubated at 37°C for 30 min with 200 nmol/L MitoTracker Green FM probes (Molecular Probes, Eugene, Ore). Samples were acquired with a Navios cytometer (Beckman Coulter).
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bioRxiv - Biochemistry 2020Quote: ... the cells were passaged 3 to 4 times in DMEM based media with same supplements as above but with HEPES based CO2-independent buffering (5958 mg/L) (Thermo Fisher Scientific, 21063045) instead of phenol red.
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bioRxiv - Microbiology 2019Quote: ... and stained with anti-LCMV-WE recombinant NP immunized rabbit serum and HRP-goat anti-rabbit IgG (H+L) DS Grd (lot: 917439A, Life Technologies, Carlsbad, CA) (41) ...
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bioRxiv - Cell Biology 2019Quote: ... To induce differentiation the cells were seeded into 24-well plates at a density of 2.5 x 104 cells/well and then shifted to DMEM with low glucose (1 g/L; Gibco, Life Technologies, cat. # 11885076) with the same FCS concentration and supplements used for BM-derived SSC differentiation.
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bioRxiv - Molecular Biology 2019Quote: Authenticated Mel202 cells were purchased from Public Health England (lot number 13H016) and were cultured in RPMI 1640 with 2mM L-glutamine and 25 mM HEPES (Gibco, Life Technologies, Paisley, UK) supplemented with 10% FCS (Sigma-Aldrich ...
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bioRxiv - Biophysics 2021Quote: HeLa cells were cultured in 75 cm2 Falcon culture flasks with supplemented RPMI Medium 1640 (1X) + L-Glutamine (Gibco® by LifeTechnologies™) containing 10% fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2020Quote: ... Ninety-six well-plates were seeded with either BT-474 or MDA-MB-231 cells at initial confluences ranging from 10% to 80% in Dulbecco’s modified eagle medium (DMEM without glucose, sodium pyruvate, HEPES, L-glutamine and phenol red, Thermo Fisher Scientific, Waltham, MA) one day before imaging experiments began ...
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Structure-guided glyco-engineering of ACE2 for improved potency as soluble SARS-CoV-2 decoy receptorbioRxiv - Biochemistry 2021Quote: ... as previously described.36,61 Cells were cultivated in FreeStyle F17 expression medium supplemented with 0.1% (v/v) Pluronic F-68 and 4 mM L-glutamine (all from Thermo Fisher Scientific, United States) in shaking flasks at 37°C ...