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Citations for Thermo Fisher :
9151 - 9200 of 9391 citations for Mumps Virus Nucleoprotein Strain L Zagreb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... CEMx174 cells in R10 (RPMI 1640 medium supplemented with 10% FBS, 1% antibiotic-antimycotic [Thermo Fisher Scientific, Waltham, MA] and 1% L-glutamine [Thermo Fisher Scientific]) in the presence of phorbol myristate acetate (PMA ...
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bioRxiv - Cancer Biology 2023Quote: ... ON-TARGETplus siRNA at a final concentration of 50 nM per plate was incubated with Dharmafect 1 at 100 nmol/L (GE Dharmacon #T-2001-03) for 20 minutes at room temperature in reduced serum Opti-MEM Medium (Thermo Fisher Scientific #31985070). siRNA+Dharmafect mixture was added to cells in Opti-MEM for 16-24 h ...
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bioRxiv - Immunology 2023Quote: ... and BMDMs (bone marrow derived macrophages) obtained by differentiation in vitro in RPMI 1640 medium with L-glutamine (Gibco Thermo Fisher Scientific 21875034) supplemented with 10% heat-inactivated fetal bovine serum (Sigma F0392 ...
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bioRxiv - Immunology 2023Quote: ... and BMDMs (bone marrow derived macrophages) obtained by differentiation in vitro in RPMI 1640 medium with L-glutamine (Gibco Thermo Fisher Scientific 21875034) supplemented with 10% heat-inactivated fetal bovine serum (Sigma F0392 ...
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bioRxiv - Cancer Biology 2022Quote: ... while the human pancreatic cancer cell line AsPC-1 (passage 11) was cultured in RPMI-1640 medium (L-glutamine supplement; Gibco, Thermo Fisher Scientific), both supplemented with 10 % (v/v ...
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bioRxiv - Microbiology 2023Quote: ... and 0.5 µl of both forward and reverse primers (5 µM) were run on the QuantStudio™ 3 Real-Time PCR System (Thermo Fisher Scientific). The primers used in this study were ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... Sections were then washed in PBS three times and incubated overnight at 4°C in goat anti-rabbit IgG (H+L) Alexa Fluor 647 secondary antibody (Invitrogen #A11036, Waltham, MA) diluted to 1:1000 in blocking ...
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bioRxiv - Immunology 2023Quote: ... 100 µL of L15C300 was added in a microtube for each tick before preparation on Fisherbrand™ Superfrost™ Plus microscope slides (Fisher Scientific) using a Epredia™ Cytospin™ 4 Cytocentrifuge (Thermo Scientific).
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bioRxiv - Cancer Biology 2023Quote: ... which was detected by the secondary antibody against a mouse IgG (H+L) F(ab’)2 fragment conjugated to AlexaFluor 647 (Molecular Probes, Thermo Fisher Scientific).
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bioRxiv - Microbiology 2023Quote: ... was used as primary antibody (dilution 1:300) and Alexa Fluor 568 labeled donkey anti-mouse IgG (H+L) (Invitrogen, cat no. A10037) as secondary antibody (dilution 1:1000).
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bioRxiv - Genomics 2023Quote: ... Aquarium ASW was supplemented three times per week with one hour of gentle bubbling of compressed carbon dioxide and dosing with 100 mL each of 20 g L-1 potassium nitrate (ThermoFisher, Waltham, MA, USA), 1.5 g L-1 potassium dihydrogen phosphate (ThermoFisher ...
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bioRxiv - Immunology 2023Quote: ... Gene expression was determined with ORA qPCR Green L mix (QPD0105, HighQu) utilizing the QuantStudio™ 5 Real-Time PCR System (Thermo Fisher Scientific) and analyzed using the delta-delta Ct method ...
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bioRxiv - Immunology 2023Quote: ... Sections were then washed 3x with PBS containing 0.05% Tween 20 and incubated with Alexa Fluorophore conjugated goat anti-rabbit IgG (H+L) secondary antibody Alexa Fluor 488 (Thermo Fisher, A-11034) and goat anti-mouse Alexa Fluor Plus 647 (Thermo Fisher ...
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bioRxiv - Immunology 2023Quote: ... cells were subsequently stained with a FITC-conjugated secondary goat anti-mouse IgG (H+L) polyclonal antibody (Invitrogen, cat# 62-6511; 1:200) or PE-conjugated F(ab’)2 goat anti-mouse IgG (H+l ...
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bioRxiv - Cell Biology 2023Quote: End1s were transfected with either an ON-TARGETplus non-targeting siRNA pool (Horizon Discovery D-001810-10-05) or an ON-TARGETplus YAP1-targeting siRNA SMARTpool (Horizon Discovery L-012200-00-0005) using Lipofectamine 3000 (Thermo Fisher Scientific L3000008), per manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... 120 µL acetonitrile was added to each supernatant sample before addition of 80 µL (200 µg) of TMT 10 plex (Thermo Scientific, cat #90110) reconstituted in acetonitrile ...
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bioRxiv - Genetics 2023Quote: ... D-001810-10) or siCHD7 (Dharmacon, L-025947-01) was diluted to 1.2 mL Opti-MEM reduced-serum medium (Thermo Fisher Scientific, 31985-070). In a separate tube ...
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bioRxiv - Molecular Biology 2022Quote: ... Secondary antibody mix was made up with donkey anti-rabbit (H+L) highly cross-adsorbed Alexa FluorTM 647 (Thermo Scientific, cat. #A-31573) diluted at 1:400 and Alexa FluorTM 594 Phalloidin (Thermo Scientific ...
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bioRxiv - Microbiology 2023Quote: ... and the pellet was resuspended in 5 mL complete RPMI (RPMI 1640 supplemented with L-glutamine, 20 mM final concentration HEPES (Gibco, Cat # 15630-080), 10% v/v final concentration heat-inactivated fetal bovine serum (FBS) ...
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bioRxiv - Cancer Biology 2023Quote: ... Sections were then incubated with the following horse radish peroxidase (HRP)-conjugated secondary antibodies for 1 hour at room temperature: goat anti-mouse IgG (H+L) (Invitrogen, 626520, 1:2000) and goat anti-rabbit IgG (H+L ...
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bioRxiv - Cell Biology 2023Quote: ... The fragments were then ligated into the vector using T4ligase (Enzymatics, # L6030-LC-L) and transformed into Max Efficiency Stbl2 Competent Cells (Thermo Fisher Scientific, #10268019). The following constructs were obtained using exactly this method:
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bioRxiv - Cancer Biology 2023Quote: ... Cells were washed 3 times in 0.25% Triton X-100 in PBS (PBS-T) and secondary antibodies: Goat anti-Rabbit IgG H+L -Alexa FluorTM 594 (Invitrogen #A11037, 1:400) or Goat anti-Mouse IgG (H+L ...
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bioRxiv - Biochemistry 2023Quote: ... Every well contained a 25 µL solution of 0.4 mg/mL of enzyme (with or without 2 mM of ADP-ribose) and SyproOrange (ThermoFisher; at 1:5000 dilution) in 50 mM HEPES pH 7.5 ...
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bioRxiv - Microbiology 2023Quote: ... we added 150 μL bacterial cultures to 350 μL of the extraction buffer (100 % Methanol + 0.14 % formic acid) in non-sterile round bottom 96-well plates (Thermo Fisher Scientific, Waltham, USA). We stored the fixed samples with a final concentration of 70 % methanol and 0.1 % formic acid at -80 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were washed with PBS followed by incubation with goat anti-rabbit IgG (H+L) Alexa fluor-594 secondary antibody (A-11012 from Thermo Scientific (Rockford, IL) was used for EphA2 ...
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bioRxiv - Biochemistry 2023Quote: Kinetic measurements of TrpBwt and its mutants were performed by monitoring 5-fluoro-L-Trp formation in a plate reader (Thermo Scientific Varioskan Lux) over 20 min at 290 nm using ΔE290 = 1.89 mM−1·cm−1 ...
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bioRxiv - Microbiology 2023Quote: ... 5 μL of the IP fraction for α-FLAG immunoblots were loaded on a 4-20% Novex WedgeWell SDS-PAGE gels (Thermo Fisher Scientific). Resolved proteins were transferred to polyvinylidene fluoride (PVDF ...
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bioRxiv - Cell Biology 2023Quote: ... cross-adsorbed Alexa Fluor 568 (A-11004) combined with goat anti-rabbit IgG (H+L) cross-adsorbed Alexa Fluor 647 (A-21244; all from Thermo Fisher, 1:400).
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bioRxiv - Cell Biology 2023Quote: ... We seeded 150 μL of zoospores suspended in Bonner’s salts into a single well of a 6-well glass bottom plate (Fisher Scientific, Cat. No. NC0452316) and allowed the cells to settle for 10 min before gradually applying confinement by decreasing the pressure from -3 kPa to -10 kPa.
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bioRxiv - Molecular Biology 2023Quote: ... larvae were euthanised in buffered MS222 (0.25 mg L-1; Ramlochansingh et al., 2014) and stored in RNAlater (Life Technologies, Carlsbad, CA, USA) at 4°C for 24 h ...
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bioRxiv - Immunology 2023Quote: Ex vivo isolated and rested CD8+ T cells or transduced T cells from splenocytes were incubated with R10 (RPMI 1640 + 10% FBS + 1% penicillin/streptomycin + L-glutamine + 20 U/ml of IL-2; Gibco, Thermo Fisher Scientific) alone or R10 containing PMA/ionomycin for two hours ...
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bioRxiv - Microbiology 2023Quote: ... 5 μL of the IP fraction for α-FLAG immunoblots were loaded on a 4-20% Novex WedgeWell SDS-PAGE gels (Thermo Fisher Scientific). For western blot analysis related to RpoD ...
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bioRxiv - Cancer Biology 2023Quote: ... media and subjected to mechanical homogenization using a plastic pestle as described previously.(26, 31, 32, 35, 36) This NSC media comprised DMEM/F12 with L-Glutamine (Gibco®, 11320-033), supplemented with 1% B27 (Gibco® ...
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bioRxiv - Cancer Biology 2023Quote: ... Secondary antibodies used for immunofluorescence were Alexa Fluor™ 488 Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody (ThermoFisher # A-11029) and Alexa Fluor™ 568 Goat anti-Rabbit IgG (H+L ...
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bioRxiv - Cancer Biology 2023Quote: ... The cells were cultured in RPMI 1640 media (ATCC 30-2001) containing 4500 mg/L glucose supplemented with 10% fetal bovine serum (FBS) and 1% antibiotic-antimycotic solution (Fisher Scientific, Boston, MA) at 37 °C in a humidified atmosphere with 5% CO2 ...
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bioRxiv - Cancer Biology 2023Quote: ... after which Matrigel would be set and organoid media (Gibco Advanced DMEM/F12, Thermo Fisher 12634010; 25% L-WRN Conditioned Medium, ATCC CRL-3276; 12mM HEPES, Thermo Fisher 15630080; 1% GlutaMax, Thermo Fisher 35050079 ...
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bioRxiv - Molecular Biology 2023Quote: ... the proteins were transferred to a nitrocellulose membrane at 20 V in the presence of transfer buffer composed of Bolt™ Transfer Buffer (20X) 50 ml/L (Thermo Fisher, BT0006), 100 ml/L methanol and water ...
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Enhancing gene transfer to renal tubules and podocytes by context-dependent selection of AAV capsidsbioRxiv - Cell Biology 2023Quote: ... 10-100 ng of tissue DNA or 1 μL of blood or urine DNA sample was mixed with Power SYBR Green PCR Master Mix (43-676-59, Fisher Scientific, Hampton, NH) and 25 pmol primers in a 25 μL reaction volume and subjected to qPCR using Rotor-Gene Q (Qiagen ...
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bioRxiv - Cell Biology 2023Quote: ... qPCRs were performed using cDNA (1:8 dilution) and the ORA™ SEE qPCR Green ROX L kit (highQu) on a PikoReal 96 machine (Thermo Fisher Scientific). GraphPad Prism was used for graphics/statistics ...
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bioRxiv - Cell Biology 2023Quote: ... 0.5 μL of probe mix (stock 25 μM) was mixed with 2 μL of salmon-sperm DNA (10 mg/ml; Life Technologies, 15632-011) and 2 μL yeast transfer RNA (10mg/ml ...
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bioRxiv - Microbiology 2023Quote: ... supplemented with heat-inactivated 10 % fetal bovine serum (FBS) and 2 mM L-glutamine (all purchased from Thermo Fisher Scientific, Waltham, MA, USA) at 37 °C in 5 % CO₂ atmosphere.
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bioRxiv - Cell Biology 2023Quote: ... The multi-color organelle suspension was mounted on poly-L-lysine-coated coverslips (Matsunami, C1110) with ProLong Gold Antifade Mountant (Thermo Fisher Scientific, P36934). For the experiment investigating mitochondria–ER contact sites ...
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bioRxiv - Cell Biology 2023Quote: ... 2 µL were injected onto a PepMap RSLC EASY-Spray column (C18, 2 µm, 100 Å, 75 µm x 15 cm, Thermo Fisher Scientific). Separation occurred at 300 nL min-1 with a flow gradient from 2-35% mobile Phase B (2% H2O ...
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bioRxiv - Physiology 2024Quote: ... 3T3L1 adipocytes were trypsin detached five days post-differentiation and reverse transfected with 50 pmols of Dharmacon SmartPool siSCR (#D-001810-10-05) or siMPC1 (#L-040908-01-0005) using Lipofectamine® RNAiMAX reagent (#13778; ThermoFisher, MA, USA) following published protocols (Kilroy et al. ...
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bioRxiv - Immunology 2024Quote: ... both cell types were resuspended in an assay medium (cDMEMlow-Glu) which contained low-glucose (1 g/L) DMEM (cat. no. 31885023, Gibco, Thermo Fisher Scientific), 10% h.i ...
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bioRxiv - Cell Biology 2024Quote: ... 100 ng/µl for Nup153-EGFP and 30 ng/μl for sfGFP-NPM-NLS-L using ultrapure water (Thermo Fisher Scientific Barnstead Smart2Pure), and an aliquot (0.3 μl ...
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Autophagy suppression in DNA damaged cells occurs through a newly identified p53-proteasome-LC3 axisbioRxiv - Cell Biology 2024Quote: ... Cell monolayers were then incubated in the presence of secondary antibody (goat anti-rabbit IgG (H+L) Alexa Fluor 488 conjugate (#A11008, Thermo Fisher Scientific, USA), or ...
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bioRxiv - Cell Biology 2024Quote: 3x106 AAVS or PS1 KO HeLa cells were plated per dish in 60 mm dishes and transfected with 10 nM of control siRNA (Dharmacon, D-001810-10-05) or TBC1D5 siRNA (Dharmacon, L-020775-01-0005) using Lipofectamine RNAiMAX Transfection Reagent (Thermo Fisher Scientific, 13778100). Cells were then treated with DMSO or 1 μM XXI for 24 h ...
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bioRxiv - Bioengineering 2024Quote: ... Samples were then rinsed with 1X PBS and incubated with Alexa Fluor-conjugated secondary antibodies: 488 Phalloidin and 594 Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody (ThermoFisher Scientific, 1:1000). All samples were counterstained with 4′,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Cancer Biology 2024Quote: ... Cat No.DSHB-S1-810-(DSHB)-E7 anti beta tubulin supernatant 1ml).Proteins were detected using Goat Anti-Mouse IgG (H+L) peroxidase Conjugates (1;2500, Thermo scientific, Cat No. 31430) and Goat Anti-Rabbit IgG-HRP (1;1000 ...