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Citations for Thermo Fisher :
8951 - 9000 of 9391 citations for Mumps Virus Nucleoprotein Strain L Zagreb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... coated 6-well plates or 18-mm round coverslips in Minimum Essential Media (MEM) with L-glutamine (Thermo Fisher Scientific, cat# 11095080), 0.6% glucose (Fisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... secondary antibodies were diluted in blocking buffer as follows: Alexa Fluor 488-Goat anti-Mouse IgG (H+L) secondary antibody (1:1000; Life Technologies; A11029) an Alexa Fluor 568-Goat anti-Rabbit IgG (H+L ...
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bioRxiv - Biochemistry 2022Quote: ... 20 μl) and analysed using an Orbitrap Fusion Lumos Tribrid Mass Spectrometer connected to a nano-LC Easy nLC 1200 (ThermoFisher Scientific, Singapore). The peptides were separated at a flow rate of 300 nL/minute on C18 pre-column (Acclaim PepMap™ 100 ...
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bioRxiv - Biochemistry 2022Quote: ... Reactions were incubated for 1 hour in blocking buffer containing goat αrabbit IgG (H+L) secondary antibody Alexa Fluor 568 conjugate (Thermo Fisher Scientific). Again ...
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bioRxiv - Cancer Biology 2023Quote: ... SMARTpool ZMIZ1 (L-007034-00)and control siRNA (D-001810-10, Dharmacon) were transfected with Lipofectamine RNAiMAX Transfection Reagent (Thermo Fisher Scientific) following the manufacturer’s protocol.
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bioRxiv - Cell Biology 2022Quote: ... human FLOT2 (ON-TARGETplus Human FLOT2 siRNA SMART POOL, L-003666-01-0010) (Horizon Discovery Ltd.), non-targeting control siRNA (#4390843, 40 nM) (Thermo Fisher Scientific). In brief ...
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bioRxiv - Cell Biology 2023Quote: HL-60 human neutrophil-like cells were obtained from O D Weiner laboratory (University of California San Francisco) and cultured in RPMI 1640 medium with L-glutamine and 25 mM HEPES (ThermoFisher Scientific; 22400089), supplemented with 15% heat-inactivated fetal bovine serum (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... 5 μl of extracted RNA samples were determined in one-step RT-qPCR using AgPath-ID one-step RT-PCR (Applied Biosystems, UK) with the following cycle conditions ...
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bioRxiv - Cell Biology 2023Quote: ... ON-TARGETplus Mouse Dlg1 SiRNAs (SMARTpool, L-042037-00-0005 were used for Unc5B gene deletion. Transfection was performed using lipofectamine RNAi max (Invitrogen, 13778-075) according to the manufacturer’s instruction with siRNA at a final concentration of 25pmol in OptiMem for 8h ...
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bioRxiv - Cell Biology 2023Quote: ... the medium was changed to Dulbecco’s Modified Eagle Medium (4.5 g/L glucose, no glutamine, no sodium pyruvate, no phenol red) (Thermo Fisher Scientific, Cat. #31053028) supplemented with 30 mM HEPES (Sigma Aldrich ...
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bioRxiv - Developmental Biology 2023Quote: ... were mechanically lysed in [100 μl/sample + 10 μl extra] lysis buffer (100 mM NaCl, 50 mM Tris, 4 mM EDTA, 1% NP40, 1 μl/ml SUPERaseIN (Thermo Fisher #AM2694), and 1x cOmplete Protease Inhibitor (Roche #11836153001) ...
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bioRxiv - Immunology 2023Quote: ... PBMCs were cultured in RPMI-1640 media supplemented with antibiotics (100 U/mL penicillin, 0.1 mg/mL streptomycin) and glutamine (2 mmol/L) purchased from Life Technologies (Waltham, USA), β2-mercaptoethanol (5×10−5 M ...
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bioRxiv - Cancer Biology 2022Quote: ... while the human pancreatic cancer cell line AsPC-1 (passage 11) was cultured in RPMI-1640 medium (L-glutamine supplement; Gibco, Thermo Fisher Scientific), both supplemented with 10 % (v/v ...
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bioRxiv - Biochemistry 2023Quote: ... and 1 µl was analyzed on a LC-MS/MS setup (UltiMate 3000 RSLCnano HPLC / QExactive HF-X mass spectrometer, both Thermo Scientific, Germany) as described (62) ...
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bioRxiv - Developmental Biology 2023Quote: ... Alexa Fluor 488 conjugated donkey anti-rat IgG (H+L) Highly Cross-Adsorbed secondary antibody (Thermo Fisher Scientific, Cat. No. A-21208), and Alexa
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bioRxiv - Cell Biology 2022Quote: ... Approximately 600 μl of the lower phase was collected into a fresh 1.5 ml tube and vacuum dried on Speedvac (Thermo Scientific. Cat# SPD140DDA) before storage at −80°C.
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bioRxiv - Physiology 2023Quote: ... the coverslips were flipped onto 60-mm dishes containing a glial cell layer in Neurobasal medium supplemented with L-glutamine (GIBCO, #25030-024) and NeuroCult SM1 Neuronal Supplement (StemCell Technologies ...
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bioRxiv - Genomics 2023Quote: Adults were relaxed by incubating for 3–16 hours at 4 °C in about 400 mL ASW with approximately 5 g of 99% L-menthol crystals (Thermo Scientific A10474) (3 adults per 400 mL volume) ...
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bioRxiv - Microbiology 2023Quote: ... ATCC TIB-202) or primary human monocytes (PHMs) were propagated in RPMI 1640 with L-glutamine and 25 mM HEPES buffer (Invitrogen, Carlsbad, CA), supplemented with 10% fetal bovine serum ...
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bioRxiv - Genomics 2023Quote: ... cells were back-diluted to OD600 of 0.3 in SCD -Met with 500 µM IAA and 50 µM L-Homopropargylglycine (HPG) (Thermo Fisher Scientific #C10186). Nascent peptide labeling was conducted for 2 hours at 30°C ...
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bioRxiv - Plant Biology 2023Quote: Click-iT detection of EdU on sections attached to poly-L-lysine coated coverslips was performed using the Click-iT cell proliferation kit (#C10340; Thermo Fisher Scientific), according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were washed three times with PBS-T and incubated with Goat Anti-Mouse IgG (H+L) HRP Conjugate (Thermo Fisher Scientific), Goat Anti-Rabbit IgG (H+L ...
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bioRxiv - Microbiology 2023Quote: ... Cells were streaked out from freezer stocks onto LB agar plates (Lennox, 20 g/l, Fisher Scientific, solidified with 1.5% (w/v) agar ...
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bioRxiv - Developmental Biology 2023Quote: ... with 10% FCS (Biosera, #FB-1280/500) and 2% glutamate/pyruvate (100mM sodium pyruvate, Invitrogen #11360-039; 200mM L-glutamine, Invitrogen, #25030-024). Slides were imaged for brightfield ...
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bioRxiv - Cell Biology 2023Quote: ... The samples were then cooled and digested with 5 μL RNase A/T1 mix (2 mg/mL of RNase A and 5,000 U/mL of RNase T1, Thermo Fisher, cat#EN0551) overnight at 37 ℃ ...
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bioRxiv - Neuroscience 2023Quote: ... then washed with PBST and incubated with goat anti-mouse IgG (H+L) Alexa Fluor 594 (1:500 dilution by the blocking solution, A-11032, Thermo Fisher Scientific) at room temperature for at least 2 hours ...
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bioRxiv - Neuroscience 2023Quote: Primary hippocampal CA1 to CA3 neurons were isolated from day 1 rat pups of mixed gender and plated on poly-l-ornithine–coated coverslips as previously described35 in minimum essential medium (Thermo Fisher 51200038) supplemented with 0.5% glucose ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were washed with 0.1% Tween in PBS and incubated with donkey anti-rabbit IgG H&L Alexa Fluor™ 568 secondary antibody (1:1000, Invitrogen, A10042) and 1 μM Hoechst prior to imaging ...
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bioRxiv - Cell Biology 2023Quote: ... in the presence of Brilliant Stain Buffer Plus (10 μl/sample) (cat# 563795, Becton Dickinson, Mountain View, Ca, USA) and CellBlox Blocking Buffer (5 μl/sample) (cat# C001T02F01, Thermo Fisher Scientific). Cells were washed again in FACS buffer and intracellularly stained to detect transcription factor expression using the True-Nuclear Transcription Factor Buffer Set (cat# 424401 ...
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bioRxiv - Synthetic Biology 2024Quote: ... were washed three times in cold flow buffer and then the secondary antibody (Goat anti-Human IgG H+L Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 488, ThermoFisher Scientific) at a dilution of 1:500 was added ...
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bioRxiv - Synthetic Biology 2024Quote: ... the cells were stained with Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody Alexa Fluor™ 488 (Invitrogen, A-11008) at dilution 1:500 for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... was expressed in Expi293 cell line (3X106 cells/ml, 200 ml in 1 L flask) using ExpiFectamine™ 293 Transfection Kit (Gibco, A14524). After 48 hours ...
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bioRxiv - Physiology 2024Quote: ... the membranes were incubated with secondary antibodies at RT for 1 hour (Goat anti-rabbit IgG (H&L) (Invitrogen; SA535571; 1:10000), Goat anti-mouse IgG (H&L ...
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bioRxiv - Physiology 2024Quote: ... Samples were washed 3 times with AbA followed by 200 μl of AbA containing Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody Alexa Fluor® 488 conjugate (dilution of 1:100, A-11008, Thermo Fisher). Samples were kept in the dark and incubated with rocking for overnight at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... 1×107 cells were seeded into 175 cm2 cell culture flasks in RPMI-1640 media with 2 mM L-glutamine (Thermo Fisher Scientific) containing 10% heat-inactivated foetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2023Quote: ... then incubated for 60 minutes at RT in donkey anti-mouse IgG (H+L) (Alexa Fluor, 647, 2ug/mL) (Invitrogen A-11032). Coverslips were washed ...
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bioRxiv - Biophysics 2023Quote: ... GPMVs were subsequently labelled with a 1:500 dilution of Alexa Fluor 488 Donkey anti-Rabbit IgG (H+L) (Invitrogen, catalog # A21206) and gently agitated in the dark at RT for a minimum of 2-3 h ...
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bioRxiv - Cancer Biology 2023Quote: ... after which Matrigel would be set and organoid media (Gibco Advanced DMEM/F12, Thermo Fisher 12634010; 25% L-WRN Conditioned Medium, ATCC CRL-3276; 12mM HEPES, Thermo Fisher 15630080 ...
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bioRxiv - Bioengineering 2023Quote: ... the secondary antibody conjugated to Alexa Fluor® 647 goat anti-rabbit IgG (H+L) (Invitrogen, cat. No. A-21245, 1:500) was then treated for 1 h to visualize the antigen ...
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Mapping chromatin state and transcriptional response in CIC-DUX4 undifferentiated round cell sarcomabioRxiv - Cancer Biology 2023Quote: ... and CIC siRNA (#L-015185-01-0005) were obtained from GE Dharmacon and transfection was performed with Lipofectamine RNAiMax transfection reagent (Thermo Fisher Scientific).
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bioRxiv - Cancer Biology 2023Quote: ... then were incubated with primary antibodies for 1 h and with either Alexa FluorTM 594-conjugated goat anti-mouse IgG (H+L; Invitrogen, #A11005, RRID:AB_2534073) or Alexa FluorTM 488-conjugated goat anti-rabbit IgG (H+L ...
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bioRxiv - Biochemistry 2023Quote: ... at a ratio of 3 μl lipofectamine LTX to 1 μl Plus reagent for every nanogram of DNA in 200 μl of Opti-MEM (Thermo Fisher Scientific). At 12 hrs post transfection cells were treated with 0.5 μM thapsigargin (Calbiochem ...
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bioRxiv - Biochemistry 2023Quote: ... The membranes were then incubated with second antibodies including Alexa Fluor™ Plus 680 Goat anti-Rabbit IgG (H+L) (Invitrogen, RRID:AB_2633283) or Alexa Fluor™ Plus 800 Goat anti-Mouse IgG (H+L ...
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bioRxiv - Biophysics 2023Quote: ... 1 L of trophozoite culture of each isolate was grown in 50 screw-cap tubes (Falcon, Thermo Fisher Scientific, Waltham, MA, USA) with TYI-S33 medium for 60 h ...
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bioRxiv - Immunology 2023Quote: ... The plates were washed and incubated with 100 μL of a 1:10,000 diluted goat anti-mouse IgG (H+L) cross-adsorbed HRP conjugate antibody (Cat. # A16078; Invitrogen, Waltham, Massachusetts, USA) for 5 minutes on an orbital shaker (300 rpm ...
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bioRxiv - Biochemistry 2024Quote: HEK293T cells used for transient transfection for thermostabilization experiments were cultured in DMEM + GlutaMAX™-I with 4.5 g/L D-Glucose and Pyruvate (Gibco 31966-021), supplemented with 10 % (v/v ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were washed 3 times with PBS and a mixture of goat anti-mouse IgG(H+L) cross-absorbed secondary antibody conjugated to Alexa FluorTM 488 (Invitrogen #A-11001) and goat anti-rabbit IgG(H+L ...
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bioRxiv - Cancer Biology 2023Quote: ... in serum-free culture medium (Advanced DMEM/F-12 supplemented with 0.6% glucose, 2 mM L-glutamine (ThermoFisher Scientific, Waltham, MA, USA), 5 mM HEPES ...
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bioRxiv - Plant Biology 2024Quote: ... 1 to 3 µl of each sample were subjected to reverse phase LC for peptide separation using an RSLCnano Ultimate 3000 system (Thermo Fisher Scientific). Peptides were loaded on an Acclaim PepMap 100 pre-column (100 µm x 2 cm ...
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bioRxiv - Developmental Biology 2023Quote: ... mSostTALEN-B-L and -R mRNAs were synthesized and a polyA tail was added using the mMESSAGE mMACHINE T7 ULTRA Kit (Ambion, Austin, TX) according to the manufacturer’s instructions ...