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Citations for Thermo Fisher :
9251 - 9300 of 9391 citations for Mumps Virus Nucleoprotein Strain L Zagreb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2020Quote: ... After washing and incubation with 1:2500 Alexa Fluor ® 568 F(ab’)2 fragment of goat anti-Rabbit IgG (H+L) (Thermo Scientific # A-21069) cells were stained with DAPI and mounted in VECTASHIELD (VectorLaboratories) ...
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bioRxiv - Microbiology 2021Quote: ... mutants were generated by transforming the generated parC mutants with the respective gyrA non-WT alleles and selecting on TSYA plates supplemented with 12 mg/L ciprofloxacin (Acros Organics, New Jersey, USA). GyrA and parC allelic replacements in the transformants were confirmed by PCR and Sanger sequencing.
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bioRxiv - Neuroscience 2021Quote: ... We then washed and incubated slices for one hour with secondary antibodies (Alexa Fluor 488 goat anti-chicken IgG (H+L) (Molecular Probes Cat# A-11039); Alexa Fluor 568 goat anti-rabbit IgG (H+L ...
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bioRxiv - Cancer Biology 2021Quote: A375 and MelJuso cells (ATCC, Manassas, VA, USA and DSMZ, Braunschweig, Germany) were maintained in RPMI-1640 with L-glutamine (Gibco, Life Technologies, Darmstadt, Germany) supplemented with 10% (v/v ...
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bioRxiv - Biophysics 2021Quote: The ovaries were removed and cleaned of extraneous tissue (uterus, oviduct, fat) using insulin needles over a heated microscope stage (37°C) in L-15 isolation media (Life Technologies, Paisley, United Kingdom) supplemented with 1% (weight/volume ...
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bioRxiv - Developmental Biology 2021Quote: ... sections were incubated for two hours at room temperature with goat anti-Rat IgG (H+L) Alexa Fluor 488 secondary antibody (ThermoFisher Scientific, diluted 1:200). Antibodies were diluted in 1×PBS with 5% BSA and incubated in the dark ...
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bioRxiv - Immunology 2020Quote: ... while the remaining adherent cells were quenched with 350 µL of ice-cold 100% methanol (Honeywell; Muskegon, MI, USA) and 350 µL of ice-cold ultrapure distilled water (Invitrogen; Grand Island NY, USA) and removed through gentle cell scraping and added to the cell pellet fraction ...
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bioRxiv - Immunology 2020Quote: ... BMDMs were seeded at 3×105 cells per well in a 24-well tissue culture plate that had been coated with poly-L-lysine (Thermo Fisher Scientific, Nepean, ON) and rinsed with PBS ...
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bioRxiv - Molecular Biology 2020Quote: ... 1%NGS+0.4%TritonX+PBS and incubated with secondary antibodies for 1 hour at room temperature with anti-mouse IgG (H+L) Alexa Fluor 555 (1:200, Thermo Fisher, Catalog# A-21422) or anti-rabbit IgG (H+L ...
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bioRxiv - Biochemistry 2022Quote: ... A baculovirus stock carrying the gene for DNMT1 was used to infect 4–12 l of Sf9 cells at a density of 2 million cells/ml in Sf-900™ III SFM (Invitrogen cat # 12658–027). Multiple 1 l cell cultures in 4 l flasks (VWR cat # 32645–044 ...
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bioRxiv - Cancer Biology 2022Quote: ... Secondary antibody incubation was conducted using Alexa Fluor 488 and goat anti-rabbit IgG (H+L) (Thermo Fisher Scientific, Waltham, MA, USA; A-11008) at a 1:1,000 dilution ...
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bioRxiv - Bioengineering 2022Quote: ... All cells were seeded in RPMI media (supplemented with 10% fetal bovine serum (FBS) and 2 mM L-glutamine (Gibco, Life Technologies, Stockholm, Sweden) and were for 48h prior experiments cultured in RPMI-media with exosome-depleted FBS (10% ...
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bioRxiv - Developmental Biology 2022Quote: ... The next day the membranes were incubated with HRP-conjugated secondary antibodies Goat anti-Rabbit IgG (H+L) (1:5000; Thermo Fisher Scientific, Cat# 31466) or Goat anti-Mouse IgG (H+L ...
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bioRxiv - Microbiology 2022Quote: ... and Alexa Fluor™ 488 Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody (1:500; Thermo Fisher Scientific cat. No. A11034). Neurons were simultaneously stained with 4=,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Plant Biology 2022Quote: ... 5 μL was analyzed by LC-MS with a Dionex RSLCnano HPLC coupled to a Orbitrap Fusion Lumos mass spectrometer (Thermo Fisher Scientific, Waltham, Massachusetts) using a 2h gradient ...
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bioRxiv - Developmental Biology 2022Quote: ... 3A9 (323 or M10-2)) with an Alexa Fluor 647 Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody (Thermo Fisher Scientific, A-31571) was used or a brightfield image was taken.
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bioRxiv - Cancer Biology 2022Quote: ... 25 µl from prepared samples were added to one well of 96 well plate and Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific, 23225) manual was followed to measure the protein content ...
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bioRxiv - Microbiology 2022Quote: ... Virus stocks and nasal washes were diluted 10-fold in 1× minimum essential medium (MEM) (10% 10× minimal essential medium [Gibco], 2 mM L-glutamine, 0.1% of sodium bicarbonate [wt/vol; Gibco], 10 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES ...
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bioRxiv - Systems Biology 2023Quote: HeLa cells and mouse embryonic fibroblasts (MEFs) were cultured in complete medium consisting of DMEM (with 4 mM L-Glutamine and 1 mM sodium pyruvate; Thermo Fisher Scientific #41966-029) supplemented with another 2 mM of L-Glutamine (Sigma #G7513 ...
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bioRxiv - Microbiology 2024Quote: ... Samples (5 µl) were injected on column and the eluate was led directly into an API 4500 triple quadruple mass spectrometer (Applied Biosystems, Foster City, CA) equipped with a turbospray ion source was operated in the positive ion mode.
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bioRxiv - Neuroscience 2024Quote: ... The slides were first washed in PBS for 30 min and then incubated with donkey anti-rabbit IgG (H+L) highly cross-adsorbed secondary antibody Alexa Fluor™ 555 or 647 (1:800; Invitrogen, Thermo Fisher Scientific) at RT for 2 hr ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were harvested by scraping in cold PBS and prepared for 1-D gel analysis using Click-IT L-azidohomoalanine protein labeling reagents (Life Technologies C10102, C10276, B10185) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... tissue was washed in PBS and immersed in goat anti-mouse or anti-rabbit IgG (H+L) cross-adsorbed Alexa Fluor™ 568 (1:500; ThermoFisher Scientific, Waltham, MA) prepared in incubation solution for 2 hours at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were eluted in 100 μl (liver) or 60 μl (stomach, duodenal mucosa) of RNase free water (Cat# 10977-015, Lot# 2186758, Invitrogen by Life technologies, Grand Island, USA). The elution step was repeated using the initial eluate to increase the RNA yield ...
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bioRxiv - Genetics 2024Quote: ... sections were treated with 500-1000 µL of 1:500 dilution (4 µg/mL) of goat anti-hamster IgG (H+L) AlexaFluor® 488 antibody (ThermoFisher Scientific catalog # A78963) diluted in 2% NGS in 1X PBS and incubated for 1 hour at RT in the dark ...
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bioRxiv - Biochemistry 2023Quote: ... the membranes were incubated with secondary antibody 1:10,000 goat anti-mouse IgG (H+L)-HRP conjugate (62-6520, Thermo Fisher Scientific Corp, Waltham MA) in 1xPBST + 6% milk powder for 30 minutes ...
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bioRxiv - Genetics 2024Quote: ... sections were incubated with 1:500 dilution (4 µg/mL) of goat anti-rabbit IgG (H+L) AlexaFluor®594 antibody (Invitrogen, catalog # A-32740) in 2% NGS in 1X PBS for one hour in the dark ...
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bioRxiv - Biochemistry 2024Quote: ... and the bound NTCP-Fab complexes were incubated with a secondary HRP-conjugated Pierce recombinant protein L (Thermofisher, 1:5000 dilution in ELISA buffer) for 30 min ...
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Development of monoclonal antibody-based blocking ELISA for detecting SARS-CoV-2 exposure in animalsbioRxiv - Microbiology 2023Quote: ... The plate was washed for three times and then added 100 uL of HRP-conjugated goat anti-feline IgG (H+L) secondary antibody (1:5000 dilution; Thermo Fisher Scientific, Waltham, MA) for incubation another hour at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... 50 μl of the suspension was transferred into each well of a microbroth dilution microtiter plate (Sensititre™ GN2F, Sensititre™ EUX2NF (Thermo Fisher Scientific)) ...
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bioRxiv - Cell Biology 2023Quote: ... and donkey anti-rabbit IgG (H+L) highly cross-adsorbed secondary antibody (Alexa Fluor Plus 555, 1:500, Thermo Fisher Scientific, A32794; RRID: AB_2762834). The finished slides were scanned with a Vectra Polaris Automated Quantitative Pathology Imaging System ...
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bioRxiv - Molecular Biology 2023Quote: ... 5μl Lipofectamine® 2000 and 100 pm siRNA were respectively diluted in 200 μL Opti-MEM™ I Reduced Serum Medium (Thermo Scientific, 31985062) for 5 min at RT ...
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bioRxiv - Molecular Biology 2023Quote: ... and slides were washed and incubated with secondary antibody: Alexa Fluor 633 conjugated goat anti-rabbit IgG (H+L) (Invitrogen #A-21070, 1:500) diluted in blocking buffer for 2 hours ...
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bioRxiv - Molecular Biology 2023Quote: ... Secondary antibody incubation was conducted using Alexa Fluor 488 and goat anti-rabbit IgG (H+L) (Thermo Fisher Scientific, Waltham, MA, USA; A-11008) at a 1:1,000 dilution ...
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bioRxiv - Cancer Biology 2023Quote: ... which was detected by the secondary antibody against a mouse IgG (H+L) F(ab’)2 fragment conjugated to AlexaFluor 647 (Molecular Probes, Thermo Fisher Scientific).
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bioRxiv - Biochemistry 2023Quote: ... fresh overnight cultures were diluted 1:100 in LB media with or without the addition of 100 ng/L aTc (Thermo Fisher Scientific, Waltham, MA). Cultures were grown at 32 °C with aeration until OD600 ~ 0.4 ...
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bioRxiv - Biochemistry 2023Quote: ... and the bound Arr3_ýC•IP6–Fab7 complexes incubated with HRP-conjugated Pierce recombinant protein L (cat: 32420, Thermofisher, 1:5000 dilution in ELISA buffer) for 30 min ...
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bioRxiv - Molecular Biology 2023Quote: ... the secondary antibodies used in this study include the following: Alexa Fluor 555 goat anti-rabbit IgG (H+L) (Invitrogen, cat # A21428, 1:1000), Alexa Fluor 555 goat anti-rat IgG (H+L ...
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bioRxiv - Microbiology 2023Quote: ... 60 L of seawater were collected from 5 m depth in the center of the Gulf of Trieste and aged in acid-washed and Milli-Q water-rinsed 20 L Nalgene carboys (ThermoFisher Scientific, Rockford, IL, USA) for about one month at room temperature in the dark ...
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bioRxiv - Cell Biology 2023Quote: ... The sponges attached to the ceramic tiles were placed in individual 1 L straight-sided polypropylene wide-mouth Nalgene bottles (ThermoFisher; cat. no. 2118-0032) filled with natural seawater ...
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bioRxiv - Biophysics 2023Quote: ... monoclonal primary antibodies for 1 h in PBST followed by incubation with HRP-conjugated anti-mouse IgG H&L (Invitrogen, Cat#A16078, 1:1000) and anti-rabbit IgG H&L (Abcam ...
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bioRxiv - Cell Biology 2023Quote: ... Cultured cells at 50% confluence were transfected with siRNA at final concentrations of 50 nmol/L using the Lipofectamine RNAiMAX transfection reagent (Thermo Fisher Scientific, Waltham, MA) according to the manufacturer’s instructions.
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bioRxiv - Genomics 2023Quote: ... were continuously passaged at 37°C under 5% CO2 in Minimal Essential Medium L-Glutamine without HEPES (MEM 1X) (Gibco™ Life Technologies GmbH, 31095052) supplemented with 10 % FBS (Life Technologies ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were then washed 3 times with PBS and then blocked for 30 minutes at room temperature with antibody blocking buffer (150 mM NaCl, 50 mM Tris-Base, 1% BSA, 100 mM L-lysine 50% normal goat serum (Thermo Fisher, Cat# 01-6201), 0.2% Triton (Roche ...
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bioRxiv - Microbiology 2023Quote: ... DNA was extracted from 100 µl of each sample in duplicate using the automated extraction “Kingfisher Flex Extraction System” and Magvet Universal Isolation kit (Thermo Fisher Scientific, LSI MV384).
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bioRxiv - Cancer Biology 2024Quote: ... Firstly proteins were precipitated with 50 μL of acetonitrile followed by steroid extraction via liquid-liquid extraction with 1 mL tert-methyl butyl ether (MTBE, Acros Organics, Fisher Scientific, UK). The MTBE layer was then removed ...
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bioRxiv - Biochemistry 2024Quote: ... All solutions were added 1:100 to double strength RPMI 2 % G supplemented with Tween-20 (RPMI 1640 with L-glutamine without Sodium Bicarbonate (Thermo Scientific, Catalog number: 31800089), 20.8 g L-1 ...
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bioRxiv - Biochemistry 2024Quote: ... cells were washed three times with TBS and incubated with appropriate secondary antibody goat anti-rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody Alexa Fluor Plus 568 (1:1,000 dilution, anti-mouse Alexa Fluor™ 568, Thermo Fisher Scientific, A-11004), were added for 1 h at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... and were activated with phytohemagglutinin-L (10 µg/mL) for 48 h and then maintained in RPMI 1640 (Thermo Fisher Scientific, Waltham, MA, USA) complete medium supplemented with rIL-2 (100 U/mL).
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bioRxiv - Microbiology 2024Quote: Iron coupons were gently rinsed with phosphate buffer (pH 7.4) and stained with Live/Dead BacLight™ bacterial staining kit (Molecular Probes L-7012, Thermo Fisher Scientific, USA) for 10 min in the dark ...