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Citations for Thermo Fisher :
8851 - 8900 of 9391 citations for Mumps Virus Nucleoprotein Strain L Zagreb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... One µl of each DNA sample was used as template for qPCR with Applied Biosystems PowerUp™ SYBR™ Green Master Mix (ThermoFisher) and primers targeting the X ...
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bioRxiv - Immunology 2020Quote: ... 10% fetal calf serum, 10 units/mL penicillin, 10 μg/mL streptomycin and 2 mM L-glutamine, all supplied by Gibco, ThermoFisher UK). To model interaction of naïve T cells and resting neutrophils ...
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bioRxiv - Immunology 2020Quote: ... One μL of the diluted libraries were used to quantify as recommended in the Quant-iT PicoGreen dsDNA Assay Kit (Thermo Fisher Scientific) with standard range from 0 to 14 ng/ well ...
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bioRxiv - Immunology 2021Quote: ... and resuspended at 1×106/mL in T-Cell Activation Medium (OpTmizer™ CTS™ T-Cell Expansion culture medium supplemented with L-glutamine/PenStrep; A1048501; Gibco). CD3+ T-cells were co-cultured in 96 well plates with CD14+HLA-DRneg/low and CD14+HLA-DRhigh monocytes at a ratio of 1 to 1 (T-cells ...
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bioRxiv - Immunology 2021Quote: ... was added to the cells in half culturing volume (40 μL added) and incubated in the dark for 5 min prior to reading on a Varioskan LUX plate reader (Thermo Fisher Scientific). Measurements were done on all sera samples from each group in at least duplicates ...
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bioRxiv - Microbiology 2021Quote: ... Samples with goat anti-hACE2 primary antibodies were first incubated with donkey anti-goat IgG (H+L) Alexa Fluor 568 (Invitrogen; A-11057) for 1 hour at RT ...
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bioRxiv - Cell Biology 2021Quote: ... Kinetoplasts and nuclei were stained with 10μL DAPI (10μg/mL) in 1xPBS for 2 minutes and slides were mounted with Slowfade® Gold antifade reagent (Invitrogen, Molecular Probes). Images were acquired on a Zeiss Axioimager Z1 using MetaMorph software and a Roper CoolSNAP HQ2 camera ...
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bioRxiv - Genomics 2021Quote: ... cells were grown at 5% CO2 and a concentration of 1X 106 cells/mL under L-glutamine-supplemented RPMI 1640 medium (11875-119, Thermo Fisher Scientific) containing 15% fetal bovine serum (35-010-CV ...
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bioRxiv - Cell Biology 2021Quote: ... Eyes were dissected from stage 27 anesthetized embryos and cultured at 20°C for 40 h in 60% L-15 and 1% antibiotic-antimycotic (Thermo Fisher Scientific).
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bioRxiv - Immunology 2021Quote: ... The Alexa Fluor secondary antibodies (488, 555, 568 and 647) used were: 488-labelled donkey anti-goat IgG (H+L) (1:500, ThermoFisher, A11055, RRID:AB_2534102); 555-labelled goat anti-rabbit IgG(H+L ...
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bioRxiv - Microbiology 2022Quote: ... and 1% of antibiotic/glutamine preparation (100 U/ml penicillin G, 100 U/ml streptomycin sulphate, and 2.9 mg/ml of L-glutamine) (Gibco-Invitrogen, Waltham, MA). A549 cells were maintained in complete media ...
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bioRxiv - Microbiology 2022Quote: ... and 1% of antibiotic/glutamine preparation (100 U/ml penicillin G, 100 U/ml streptomycin sulphate, and 2.9 mg/ml of L-glutamine) (Gibco-Invitrogen, Waltham, MA).
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bioRxiv - Neuroscience 2022Quote: ... Cells were pelleted by 4 min centrifugation at 800 rpm at room temperature and resuspended in DMEM/BSA (DMEM/F-12 without phenol red, with L-glutamine and 10 mM HEPES, 0.1% bovine serum albumin; Gibco®, Thermo Fisher Scientific) at a concentration of 5×106 cells/ml ...
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bioRxiv - Biochemistry 2022Quote: ... Samples were either directly injected from the 384 well plate or transferred to a PEG coated PCR tubes (PCR-02-L-C, Axygen®) or to the autosampler glass vials (601801655, Thermo Scientific).
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bioRxiv - Biochemistry 2022Quote: ... 20 ml of the pre-culture were used to inoculate two liters of pre-warmed Terrific Broth (TB) medium supplemented with 50 μg/ml kanamycin sulfate in a 2-l flask (Thermo Fisher Scientific). The culture was stirred with 750 rpm and cultivated at 37°C until it reached an OD600 of 0.6–0.8 ...
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bioRxiv - Biophysics 2022Quote: ... were cultured in Dulbecco’s modified Eagle’s medium (DMEM)/F-12 mixture medium with L-glutamine and HEPES (1:1; 11330-032, Gibco, Thermo Fisher Scientific) at 37°C under 5% CO2 with 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2022Quote: ... cells were incubated at 37°C and 10% CO2 in “full media” consisting of a RPMI 1640 with L-Glutamine (RPMI, Gibco, 11875-093) supplemented with 10% heat-inactivated fetal bovine serum (hi-FBS ...
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bioRxiv - Microbiology 2022Quote: ... or 60 pmol of siRNA for c-Jun (Dharmacon ID L-003268-00-0010) was diluted in Opti-MEM (Invitrogen, Carlsbad, CA). Separately ...
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bioRxiv - Genomics 2022Quote: ... K562 cells were maintained at 37°C and 5% CO2 in R10 medium (RPMI with stabilized L-glutamine (Thermo Fisher, no. 11875119) supplemented with 10% FBS).
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bioRxiv - Microbiology 2022Quote: Post-RCA DNA was diluted to a volume of 50 µL to reduce viscosity of the samples and then the concentration of DNA was assessed by Qubit 4 Fluorometer (Thermo Fisher Scientific). Post-RCA DNA was library-prepped using the Nextera DNA kit (Illumina) ...
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bioRxiv - Microbiology 2022Quote: ... They were then washed with PBS three times and incubated with secondary antibody: either Alexa Fluor 488 Goat Anti-Rabbit IgG (H+L) antibody (Thermofisher; A-11008) or Alexa Fluor 647 Donkey anti-Rabbit IgG (H+L ...
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bioRxiv - Microbiology 2022Quote: ... with elution in twice 15 µl ddH2O and the entire reaction was transformed into 100 µl of ElectroMax DH5α-E Competent Cells (Cat# 11319019, Thermo Fisher Scientific) as described above ...
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bioRxiv - Microbiology 2022Quote: ... diluted into to 500 μl of 107 cfu/ml in the wells of an 8-well Nunc Lab-Tek II chamber cover glass (Thermo Fisher Scientific). The cover glass was covered with the provided cap and incubated at 28°C for 72 h ...
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bioRxiv - Microbiology 2022Quote: ... 5-10 μL was pipetted onto a microscope slide and pictures were taken on an EVOS FL Cell Imaging System (Life Technologies/Thermo Fisher) at 40x magnification ...
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bioRxiv - Microbiology 2022Quote: ... Cells were again centrifuged and washed with 1ml of 1x PBS before incubation with 1/500 diluted Goat anti-rabbit IgG (H+L) Alexa 555 (Thermo Fisher Scientific) in 100μl 1x PBS at 37°C for 1h ...
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bioRxiv - Systems Biology 2022Quote: ... Transfection reagent and media were removed from LX293T cells approximately 16 hours later and transfected cells were re-fed with 1 g/L glucose DMEM (Life Technologies 11885076), 1% Antibiotic-Antimycotic (Thermo 15240062) ...
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bioRxiv - Neuroscience 2022Quote: ... Neurons were seeded at 50×103 per cm2 on plates coated with poly-L-ornithine and laminin in Neurobasal Plus medium containing 2% B27 Plus supplement (Life Technologies, USA). On day 2 ...
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bioRxiv - Neuroscience 2023Quote: ... slides were washed in PBS three times and incubated in goat anti-chicken (H&L) Alexa Fluor 488 (A11039; Invitrogen, Thermo Fisher Scientific) goat anti-mouse IgG1 Alexa Fluor 555 (A21127 ...
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bioRxiv - Plant Biology 2023Quote: All micrographs of PpPSI-L were collected using SerialEM software [52] on a 300 kV Titan Krios electron microscope (Thermo Fisher Scientific) equipped with a Gatan K2 Summit direct detector camera and a GIF quantum energy filter (20 eV) ...
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bioRxiv - Neuroscience 2023Quote: ... followed by a 1 h incubation with goat anti-rabbit IgG (H+L) conjugated with Alexa fluor 488 (A32731; 1:1000; Invitrogen, Waltham, MA). The sections were then incubated with GFAP antibody (mouse anti-GFAP ...
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bioRxiv - Microbiology 2024Quote: ... probed with anti-GFPmut2 primary antibody (1:1000 dilution, rabbit pAb, custom Pocono RF&L) with anti-rabbit conjugated to HRP (1:1000 dilution, Invitrogen, Cat #A16104) or streptavidin-HRP (1:1000 dilution ...
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bioRxiv - Cancer Biology 2024Quote: ... PtenpeΔ/Δ and PtenpeΔ/Δ;L-gp130peKI/KI mice was done as previously described80 and magnetic cell separation (MagniSort technology, Thermo Fisher) was performed for EpCAM positive fraction using anti-mouse CD326 (EpCAM ...
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bioRxiv - Neuroscience 2022Quote: ... and at 30 DIV by neurosphere medium (Advanced DMEM/F12, 1x B27 -RA, 1x L-glutamate, 1x NEAA, 1x P-S [all from Thermo Fisher Scientific]) supplemented with 20 ng/ml bFGF2 and 20 ng/ml epidermal growth factor (EGF ...
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bioRxiv - Biochemistry 2022Quote: ... which were supplemented with additional 800 μL of RIPA buffer and incubated overnight at 4°C with 15 μL of antibody-coupled protein G Dynabeads (Thermo Fisher Scientific). Before adding the cleared chromatin ...
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bioRxiv - Developmental Biology 2022Quote: ... the spheroids were plated on Poly Ornithine/Laminin/ Fibronectin (PO/LM/FN) coated dishes (prepared as described previously (17)) in Neurobasal (NB) medium supplemented with L-Glutamine (Gibco, 25030-164), N2 (Stem Cell Technologies ...
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bioRxiv - Microbiology 2024Quote: ... Drexel University) at 1:500 followed by goat anti-mouse IgG H+L Alexa Fluor™Plus 488 (Invitrogen, A32723, Lot # SG251135) at 1:500 and goat anti-rabbit IgG H+L AlexaFluor™ 568 (Invitrogen ...
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bioRxiv - Molecular Biology 2024Quote: Primary dermal fibroblasts were from a forearm skin punch biopsy from the proband and cultured in Dulbecco’s Minimal Essential Media (DMEM) containing 4.5 g/L glucose supplemented with 10% heat inactivated fetal bovine serum and 1% antibiotic-antimycotic (Life Technologies, Carlsbad, CA). Control cells obtained from Coriell Institute (Camden ...
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bioRxiv - Bioengineering 2024Quote: ... and 800 μL of a viral solution comprising concentrated lentiviral particles and 8 μg/mL polybrene was added to the cells (Thermo Fisher Scientific). Cells were then spinfected at 1048 × g for 90 min at 30 °C ...
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bioRxiv - Cell Biology 2024Quote: ... Each siRNA construct was transfected at 220 pmol/l precomplexed and incubated in the transfection reagent in Opti-MEM (Gibco, ThermoFisher Scientific) for 4 h ...
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bioRxiv - Neuroscience 2024Quote: ... The cDNA products were diluted 1:1 and 2 µL was analyzed by qPCR using custom primer sets and PowerUp SYBR Green Master Mix (10 µL total reaction, Applied Biosystems # A25742). RT-qPCRs were performed on a Quantstudio3 from Applied Biosystems ...
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bioRxiv - Immunology 2023Quote: ... and MC38-Ova (obtained from AstraZeneca) murine colon adenocarcinoma cells were cultured in DMEM and supplemented with 2mM L-glutamine (Thermo Fisher Scientific), 10% FBS (F9665 ...
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bioRxiv - Biochemistry 2024Quote: The samples were analyzed by injecting 5 μl of the extracted solutions into a Dionex Ultimate 3000 ultra-HPLC system connected to a photodiode array detector (Thermo Fisher Scientific), with a reverse-phase column (ZORBAX Eclipse Plus C18 ...
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bioRxiv - Biophysics 2024Quote: ... Fungal conidia were cultivated at 30°C for one week on the agar media prepared with 65 g/L YPD (Catalogue # DF0428175, Thermo Fisher Scientific) and pre-autoclaved ...
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bioRxiv - Biophysics 2024Quote: Cells were cultured in either RPMI with L-glutamine (FisherScientific, cat# 12004997)—for suspension cells—or DMEM with high glucose (ThermoFisher, cat# 11965092)—for adherent cells—supplemented with 10% Fetal Bovine Serum (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were washed in PBS and then incubated for 1 h in 1:500 diluted goat anti-chicken IgG (H+L) cross-adsorbed secondary antibody (Alexa fluor 488; Fisher Scientific A11039). The mounted slices were imaged using a Leica SP8 X confocal microscope equipped with a 63x 1.3 NA glycerol immersion objective.
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bioRxiv - Systems Biology 2024Quote: ... Cells were cultured in a water-saturated incubator at 37 °C with 5% CO2 in RPMI-1640 containing L-glutamine (Gibco, catalog # 11875093) supplemented with 10% fetal bovine serum (ATCC ...
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bioRxiv - Physiology 2024Quote: ... 2 µl cDNA was amplified with TaqMan™ qPCR (38 cycles, 3 technical replicates of 3 biological replicates) using master mix (Thermo Fisher) and the following TaqMan assays ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were dispersed by titration with fire-polished Pasteur pipettes in a solution composed of two media combined in a 1:1 ratio: Leibovitz’s L-15 (Invitrogen, Grand Island, NY) supplemented with 5 mM HEPES ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were washed x3 with TBS-T for 5 minutes and treated with goat anti-mouse IgG (H+L) secondary antibody HRP (1:10000; 31430; Invitrogen, Waltham, MA) or goat anti-rabbit IgG (H+L ...
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bioRxiv - Genomics 2024Quote: ... Bacteria were harvested by adding 4 mL LB to each dish and scraping with an L-shaped cell spreader (Thermo Fisher Scientific). Plasmid DNA was isolated from the bacteria using PureLink HiPure Plasmid Filter Miniprep Kit (Thermo Fisher Scientific ...