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Citations for Thermo Fisher :
9201 - 9250 of 9391 citations for Mumps Virus Nucleoprotein Strain L Zagreb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... First antibody incubation was done O/N at 4°C and the secondary HRP-coupled antibodies goat anti-Mouse IgG (H+L) (ThermoFisher scientific; 31430; 1:10,000) or polyclonal HRP-conjugated goat-anti-rabbit IgG (ThermoFisher scientific ...
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bioRxiv - Cell Biology 2019Quote: ... 4 mM L-glutamine, 0.6% penicillin and streptomycin. A549 cells (ATCC, Cat. No. CRL-1651) were maintained in DMEM (Thermo Fisher Scientific, Cat. No. 10566016), supplemented with 10% Fetal Bovine Serum (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... Membranes were then washed in PBST and incubated with secondary antibody anti-mouse IgG (H+L) coupled to horseradish peroxidase (1/20000; ThermoFisher Scientific Cat# 32230, RRID:AB_1965958). Proteins were revealed by SuperSignal™ West Pico Chemiluminescent Substrate (ThermoFisher Scientific Cat# 34580 ...
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bioRxiv - Cell Biology 2020Quote: ... the cells were incubated for 1 hour at RT with goat anti-mouse and goat anti-rabbit IgG (H + L) secondary antibodies conjugated to fluorophores (Alexa Fluor 488 nm, 568 nm and/or 594 nm; 1:500; Life Technologies, Carlsbad, CA, USA). Unbound antibodies were rinsed and conjugated fluorophores protected with Fluoromount-G (Southern Biotech ...
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bioRxiv - Immunology 2021Quote: ... Cells were cultured at 35 °C with 5% CO2 in Gibco™ RPMI 1640 medium with L-glutamine (Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% heat-inactivated fetal bovine serum (Bovogen Biologicals ...
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bioRxiv - Developmental Biology 2021Quote: Fibroblasts were cultured in Dulbecco’s Modified Eagle’s medium (DMEM) containing 1 g/L glucose supplemented with 10% heat-inactivated fetal bovine serum (FBS) and 1% antibiotic-antimycotic (Life Technologies, Carlsbad, CA, USA).
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bioRxiv - Developmental Biology 2021Quote: ... Secondary antibodies and stains: Goat anti-Rabbit IgG (H+L) Alexa 488 and 594 (1:1000 cells, 1:500 tissue Invitrogen; A-11008, A-11012), Goat anti-Mouse IgG1 Alexa 488 (1:500 Invitrogen ...
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bioRxiv - Neuroscience 2021Quote: ... transfected cells were collected in bovine serum albumin (BSA) medium (DMEM/F12 without phenol red with L-glutamine and 15 mM HEPES, Gibco, supplemented with 0.1% BSA), at a density of 5 million cells per mL ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were transfected with non-targeting siRNA (Dharmacon, cat# D-001810-10-05) or siRNA targeting Cdkn1a (Dharmacon, cat# L-058636-00-0005) using Lipofectamine RNAiMAX (Thermo Scientific, cat# 13778-075) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... and 1 µl of 100 µM bottom splint (IDT) diluted in 7 µl nuclease free H2O (DNase/RNase-Free Distilled H2O, Thermo Fisher Scientific #10977-049). The reaction was annealed at 95 °C for 1m following 20 s at 95 °C and decreasing temperature by 1 °C every cycle ...
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bioRxiv - Developmental Biology 2020Quote: ... Biopsies were performed randomly in the menstrual cycle and biopsied material was immediately suspended in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 phenol red-free medium (DMEM/F12; L-glutamine, HEPES; Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 1% penicillin/streptomycin at 37°C ...
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bioRxiv - Developmental Biology 2020Quote: ... and incubated for 30 minutes at room temperature with a 1:1000 dilution of donkey anti-rabbit IgG (H+L) secondary antibody conjugated to Alexa Fluor® 647 (ThermoFisher-Invitrogen, catalog A-31573) in blocking solution ...
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bioRxiv - Developmental Biology 2019Quote: ... FACS-purified CD31+CD146+ primed vs naïve VP populations were expanded in EGM2 medium ∼7 days to 60 to 70% confluency on fibronectin pre-coated 6-well plates (1-1.5 x 105 cells/well) prior to Dil-Ac-LDL uptake assays (Life Technologies, Cat No. L-3484). Fresh EGM2 medium supplemented with 10 μg/mL Dil-Ac-LDL ...
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bioRxiv - Cell Biology 2019Quote: ... Live-cell imaging experiments were performed using 8-well ibidi chambers (Vitaris, Switzerland) and imaging medium (Leibovitz L-15 medium from Thermofisher, supplemented with 10 % FCS). The following drug concentrations were used ...
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bioRxiv - Neuroscience 2020Quote: ... sections were incubated for 1 hour at room temperature in darkness with appropriate secondary antibodies: Alexa Fluor 488 donkey anti-mouse IgG (H+L) (1:400, A21202, Thermo Fisher Scientific, Mass., USA), Alexa Fluor 555 donkey anti-rabbit IgG (H+L ...
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bioRxiv - Bioengineering 2021Quote: ... Samples were washed three times with 0.05% Tween 20/PBS and then incubated for one hour in the secondary antibody Alexa Fluor® 488 goat anti-Mouse IgG / IgA / IgM (H+L) (Molecular probes 1:500) combined with the secondary antibody Alexa Fluor® 594 goat anti-Rabbit IgG (H+L ...
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bioRxiv - Biochemistry 2020Quote: ... with a flow rate of 5 μ l/min and separated on an EASY-Spray HPLC Column (75 μ m × 150 mm, Thermo Fisher Scientific). HPLC gradient was conducted using 5-55% of the mobile phase (75% acetonitrile and 0.1% formic acid ...
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bioRxiv - Cell Biology 2021Quote: ... cells were incubated for 1 hour at RT with goat anti-mouse and goat anti-rabbit IgG (H + L) secondary antibodies conjugated to fluorophores (Alexa Fluor 488 nm, 568 nm and/or 594 nm; 1:500; Life Technologies, Carlsbad, CA, USA). Unbound antibody was rinsed ...
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bioRxiv - Plant Biology 2021Quote: Ten μL of filtered extract were injected in a U-HPLC-MS system consisting of an Accela Series U-HPLC (ThermoFisher Scientific, Waltham, MA, USA) coupled to an Exactive mass spectrometer (ThermoFisher Scientific ...
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bioRxiv - Plant Biology 2021Quote: ... The ion concentrations of the collected supernatant samples were then determined using a Thermo Scientific ICS-5000+ ion chromatographic system using 500 L of the sample (Thermo Fisher Scientific, MA, USA).
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bioRxiv - Neuroscience 2020Quote: ... Microglia were cultured as adherent cells in poly-L-lysin coated T75 tissue culture flasks in microglia culture medium containing DMEM/F12 (Gibco™, Thermo Fisher Scientific), 10% fetal calf serum (Biochrom ...
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bioRxiv - Immunology 2020Quote: ... the plates were washed 5 times and incubated with goat anti-human IgG (H+L) antibody conjugated with ALP (Thermo Fisher, a18808, 1:5000) for 30 min at 37 °C ...
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bioRxiv - Neuroscience 2020Quote: ... sections were incubated for 1 hour at room temperature in darkness with appropriate secondary antibodies: Alexa Fluor 488 donkey anti-mouse IgG (H+L) (1:400, A21202, Thermo Fisher Scientific, Mass., USA), Alexa Fluor 555 donkey anti-rabbit IgG (H+L ...
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bioRxiv - Microbiology 2021Quote: ... natural kaolinite solution in water were autoclaved and spread over the agar surface using a disposable L shaped spreader (Thermo Fisher Scientific, Waltham, MA). The solution was dried for 1 h to create a thin mineral film on the agar surface ...
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bioRxiv - Microbiology 2020Quote: ... 50 μl of each single cell suspension (effector and target cells) was added to the wells using a Multidrop dispenser (Thermo Scientific, Waltham, MA, USA). After incubation at 37°C for 4 h ...
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PSGL-1 inhibits HIV-1 infection by restricting actin dynamics and sequestering HIV envelope proteinsbioRxiv - Microbiology 2020Quote: ... The fluorescent secondary antibodies used in this study were Alexa Fluor 568 goat anti-human IgG(H+L) (Molecular Probes, A-21090, 1:1000), Alexa Fluor 488 goat anti-mouse IgG(H+L ...
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bioRxiv - Cell Biology 2020Quote: ... Wells with colonies were allowed to grown to confluency before initiating differentiation for myogenic (DMEM (cat nb Cat. no. 11965092, Thermofisher, 4.5 g/L glucose), 5% FBS ...
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bioRxiv - Cell Biology 2021Quote: ... with L-Glutamine, Phenol Red and Sodium Pyruvate (FUJIFILM Wako chemicals, 043-30085) supplemented with 10% fetal bovine serum (FBS) (Gibco, 12483, Lot No.1010399) and Penicillin-Streptomycin (Gibco ...
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bioRxiv - Bioengineering 2022Quote: ... Levels of NIC in supernatants were determined by analyzing 2 μL of the supernatants using a Waters ultrahigh performance liquid chromatography system (UPLC; Milford, USA) coupled with an API400 (Applied Biosystems/MDS Sciex, USA)) in tandem mass spectrometry mode (LC-MS/MS) ...
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bioRxiv - Microbiology 2022Quote: ... The pelleted DNA was resuspended in 100 μ l Tris-EDTA buffer (10 mM Tris-Cl, 1 mM EDTA) containing 100 μ g ml-1 RNase A (Thermo Fisher Scientific) and incubated at room temperature for 5 min ...
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Construction of a constitutively active type III secretion system for heterologous protein secretionbioRxiv - Synthetic Biology 2022Quote: ... The anti-FLAG antibody was subsequently detected with goat anti-mouse IgG (H+L) conjugated to horseradish peroxidase (HRP) to facilitate chemiluminescent detection (Invitrogen 32430, 1:1,000 dilution). Chemiluminescent signal was produced with Pierce ECL substrate (Thermo Scientific) ...
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bioRxiv - Bioengineering 2022Quote: ... Devices were washed with PBS for 4-6 hrs and subsequently incubated with Alexa Fluor 647-conjugated goat anti-mouse IgG (H+L) (ThermoFisher cat# A32728, 1:300) and Hoechst 33342 (Sigma-Aldrich cat# B2261 ...
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bioRxiv - Cancer Biology 2022Quote: ... Membranes were washed with PBST and incubated with goat anti-mouse IgG (H+L) secondary antibody conjugated with horseradish peroxidase (Thermo Fisher, 31430, 1:10,000) on a shaker at room temperature for 1 hour ...
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bioRxiv - Cell Biology 2022Quote: ... The genomic DNA was used as a template for a PCR reaction with the appropriate primers (sequences provided in Table 1) and the Phire Green Hot Start II DNA polymerase (F124-L, Thermo Fisher Scientific, MA, USA). The PCR products were visualised on 1-2 % agarose gels using a Bio-Rad ChemiDoc imaging system.
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bioRxiv - Microbiology 2020Quote: ... cells were washed twice and stained with 1:50 of goat anti-rabbit IgG (H+L) Alexa Fluor 647 (Thermo Fisher Scientific Cat#A21244) for 30 minutes at 4°C followed by two washes and diluted in 100 μL of staining buffer ...
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bioRxiv - Microbiology 2020Quote: Vero cells were seeded at 1.2 × 104 cells per well in Opti-PRO™ SFM supplemented with 4 mM L-glutamine and 1X Antibiotic-Antimycotic solution (Gibco/Thermo Fisher Scientific) in black ...
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bioRxiv - Molecular Biology 2020Quote: ... For co-immunoprecipitation same amounts of total RNA (about 2.5 μg) were mixed with 10 μ L of Streptavidin ™ MyOne ™ Dynabeads C1 (Thermo Fisher Scientific) processed exactly as described in Fazal et al. ...
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bioRxiv - Immunology 2022Quote: ... Macrophages were cultured in complete RPMI media (cRMPI; RPMI 1650, L-glutamine, 10% heat-inactivated fetal bovine serum (FBS; Gibco, Thermo Fisher Scientific, Rockford, IL), 1% penicillin/streptomycin ...
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bioRxiv - Immunology 2022Quote: ... Then 200 µl of the cytoplasmic fraction was processed for genomic DNA extraction using the commercial PureLink™ genomic DNA Mini kit (Invitrogen, Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... For immune phenotyping 106 fresh PBMC were surface stained in a 25 µl antibody mix containing a LIVE/DEAD™ fixable near-IR-dead cell staining reagent (1:200 dilution, cat. L10119, Invitrogen, Carlsbad, CA, USA) with combinations of the listed antibodies (Extended Data Table 1. ...
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bioRxiv - Biochemistry 2022Quote: ... Prostate cancer cell lines were cultured in RPMI medium supplemented with 2 mM L-glutamine and 10% heat-inactivated FCS (ThermoFisher Scientific, Melbourne, VIC, Australia). Cell media was changed every three days and cells were passaged regularly at ∼80-90% confluency by trypsinization ...
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bioRxiv - Biochemistry 2019Quote: ... The samples were reduced with 10 µL of 200 mmol/L Tris(2-carboxyethyl)phosphine hydrochloride (TCEP, Thermo Scientific; final concentration of 20 mM) and incubated at room temperature for 30 min ...
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bioRxiv - Plant Biology 2020Quote: ... Prior to mass analysis samples were reconstituted in 100 µl of 25% acetonitrile/water (v/v) and filtered using a micro-spin 0.2 µm nylon membrane filter (Thermo Fisher Scientific, Waltham, MA, USA).
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bioRxiv - Immunology 2020Quote: ... 1.5 g/L sodium bicarbonate supplemented with 10% heat-inactivated fetal bovine serum and 1% streptomycin and penicillin (Life Technologies, Grand Island, NY, USA). Human primary bronchial epithelial cells were purchased from PromoCell (Heidelberg ...
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bioRxiv - Immunology 2019Quote: ... Each experiment was performed after overnight resting of thawed and washed PBMCs in RPMI 1640 supplemented with L-glutamine (Life Technologies Corporation, Carlsbad, USA), 10% fetal calf serum (Gibco ...
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bioRxiv - Cancer Biology 2019Quote: ... were purchased from Dharmacon (Thermo Scientific, Hemel Hempstead, UK. Cells were transfected with 25 nmol/L siRNA using Lipofectamine RNAiMax transfection reagent (Invitrogen, Life Technologies, Paisley, UK), following the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2020Quote: ... The top clear aqueous layer (350μL) was harvested and combined with 350 μl of 70% Ethanol (1:1) and applied to Purelink RNA columns (ThermoFisher, Life Technologies, cat#12183025). RNA was then purified following manufacturer’s protocol and incorporated the addition of DNase (ThermoFisher ...
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bioRxiv - Biochemistry 2019Quote: ... Blots were incubated over-night with primary antibody diluted in PBST (phosphate buffered saline with 1 mL/L Tween-20/PBS) + 5 % BSA (Thermo Fisher Scientific, Roskilde, Denmark) at 4°C ...
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bioRxiv - Neuroscience 2019Quote: ... Samples were finally washed with ChP epithelial cell (CPEC) medium (DMEM+10%FBS+1xPen/strep) and re-suspended in 1xHBSS + glucose + LIVE/DEAD staining kit (ThermoFisher, Catalog: L-3; 224). Live cells identified by green fluorescence (calcein-AM ...
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bioRxiv - Molecular Biology 2019Quote: Authenticated Mel202 cells were purchased from Public Health England (lot number 13H016) and were cultured in RPMI 1640 with 2mM L-glutamine and 25 mM HEPES (Gibco, Life Technologies, Paisley, UK) supplemented with 10% FCS (Sigma-Aldrich ...