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Citations for Thermo Fisher :
9001 - 9050 of 9391 citations for Mumps Virus Nucleoprotein Strain L Zagreb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: HeLa cells were grown in Dulbecco’s Modified Eagle Medium (DMEM) with glutaMAX™ additive and 4.5 g/l glucose (Thermo Fisher Scientific, USA). The culture medium was supplemented with 1% (v/v ...
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bioRxiv - Immunology 2024Quote: ... both cell types were resuspended in an assay medium (cDMEMlow-Glu) which contained low-glucose (1 g/L) DMEM (cat. no. 31885023, Gibco, Thermo Fisher Scientific), 10% h.i ...
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bioRxiv - Cell Biology 2024Quote: ... at a flow rate of 0.3% µL/min and separated on a C18 analytical column (75 µm × 50 cm; PepMap RSLC C18; P/N ES803; Thermo Fisher Scientific). Peptides were then electrosprayed using 2.3 kV into the ion transfer tube (300° C ...
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bioRxiv - Cell Biology 2024Quote: The desired number of wells in a 96-well plate (MGB096-1-2-LG-L; Matrical, Inc.) or an 8-well Lab-Tek II chamber (155409; Thermo Fisher Scientific) were coated with 100 µL or 200 µL of 40 µg/µL porcine fibronectin (prepared from whole blood ...
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bioRxiv - Immunology 2024Quote: ... wells were washed three times with SIFA buffer and secondary antibody Alexa Fluor® 488 Goat Anti-Mouse IgG (H+L) (Invitrogen#A11001) diluted 1/200 added for a 1-hour incubation step on ice ...
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bioRxiv - Cell Biology 2024Quote: ... were cultured in Dulbecco’s Modified Eagle Medium (DMEM) with 4.5 g/L glucose and supplemented with 10 % Fetal Bovine Serum (FBS) (ThermoFisher Scientific, Waltham, USA) and were kept at 37 °C and 5 % CO2 ...
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bioRxiv - Neuroscience 2024Quote: ... the following secondary antibodies were used: goat anti-rabbit IgG (H+L) coupled to Alexa Fluor 488 (A-11034, Thermo Fisher Scientific), Alexa Fluor 546 (A-11035 ...
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bioRxiv - Neuroscience 2024Quote: ... They were then treated with donkey anti-mouse IgG (H + L) Alexa Fluor secondary antibodies (1:200; Alexa FluorTM 647, catalog no. A31571, ThermoFisher Scientific, USA) and DAPI (1:5000 ...
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bioRxiv - Neuroscience 2024Quote: ... They were then treated with donkey anti-rabbit IgG (H + L) Alexa Fluor secondary antibodies (1:200; Alexa FluorTM 555, catalog no. A31572, ThermoFisher Scientific, USA) and DAPI (1:5000 ...
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bioRxiv - Neuroscience 2024Quote: NeuN IF in the S1 were used to assess off-targeting binding of a Dk anti-Ms IgG (H+L) secondary Ab (A21202, Thermo Fisher Scientific) and a Dk anti-Rb IgG (H+L ...
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bioRxiv - Microbiology 2024Quote: ... as commercial OptiMEM already contains HEPES at half of the concentration in RPMI medium (2400 mg/L) (Thermo Fisher Scientific, DE, 2020).
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bioRxiv - Microbiology 2024Quote: ... The TMT-labeled peptide samples were loaded (2 µl) onto a C18-reversed phase Easy Spray column (50 cm long, 75 µm inner diameter, 2 µm particles; Thermo Fisher Scientific) with 100% nano buffer A ...
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bioRxiv - Cancer Biology 2024Quote: SNU1967 cells were transfected with ON-TARGETplus Human siRNA SMARTpool targeting SOX9 (L-021507-00-0005) using Lipofectamine RNAiMAX (Thermo Fisher Scientific). Post-transfection ...
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bioRxiv - Neuroscience 2024Quote: ... were supplemented to customized RPMI media lacking L-Tryptophan (Cat no. 72400120, Customized RPMI 1640 Medium + GlutaMAX supplement + HEPES, ThermoFisher, Pittsburgh, PA). For steady state labeling of metabolites [U-13C] Tryptophan (0.025 mM) ...
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Cellular sialoglycans are differentially required for endosomal and cell-surface entry of SARS-CoV-2bioRxiv - Microbiology 2024Quote: ... CR3022 antibody (10 μg/mL) and 10 μg/mL of goat anti-human IgG (H+L) secondary antibody Alexa Fluor 647 (Invitrogen #A-21445) was pre-incubated for 30 min at 4 °C ...
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bioRxiv - Neuroscience 2024Quote: ... Secondary antibodies used were: (1) Alexa Fluor Plus 488 Goat anti-Mouse IgG (H+L) (for PV stain; catalog # A32723, Invitrogen; RRID: AB_2633275), (2 ...
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bioRxiv - Molecular Biology 2024Quote: ... with 100 nM of either ON-TARGETplus Non-targeting Control siRNA Pool (Dharmacon #D-001810-10-05) or On-TARGETplus Smartpool siRNA targeting rat ACLY protein (Dharmacon #L-089773-02-0005) using lipofectamine RNAimax (Thermofisher Scientific #13778075), as per manufacturer’s protocol.
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bioRxiv - Molecular Biology 2024Quote: ... The medium was adjusted to pH 5.8 using 1N KOH prior to addition of 6 g/L Phytagar (Gibco, Life Technologies, U.S.A) and autoclaving for 20 min at 121°C ...
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bioRxiv - Neuroscience 2024Quote: ... followed by an intraperitoneal injection of azidohomoalanine (AHA) (100 μg/g body weight, i.p., Click-IT™ AHA [L-Azidohomoalanine], Cat No. C10102, Thermo Fisher Scientific). After 3 h ...
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bioRxiv - Biophysics 2021Quote: MDCK-II wild type canine kidney epithelial cells were a gift from the Nelson Laboratory at Stanford University and were cultured in customized media consisting of low-glucose (1 g/L) DMEM with phenol red (Gibco, USA; powder stock), 1 g/L sodium bicarbonate (lower than standard DMEM) ...
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bioRxiv - Biophysics 2021Quote: ... the samples were incubated with the appropriate secondary antibody (polyclonal goat anti-mouse IgG1, 1:500, ThermoFisher A21124; polyclonal goat anti-rabbit IgG H&L, 1:500, ThermoFisher A11011, Waltham, USA) diluted in blocking solution for 45 min at room temperature ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were washed twice with the PBSA buffer and incubated with the Alexa Fluor® 488 Donkey Anti-rabbit IgG (H+L) Antibody (Thermo Fisher Scientific) at 1:500 dilution for 30 min at room temperature ...
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bioRxiv - Cell Biology 2019Quote: ... or 0.25 μl of oligo dT30 (stock 50 μM) was mixed with 2 μl of salmon-sperm DNA (10 mg/ml, Life Technologies, 15632-011) and 2 μl yeast transfer RNA (10 mg/ml ...
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bioRxiv - Cell Biology 2020Quote: ... the membrane was incubated with secondary antibody anti-mouse IgG (H+L) coupled to horseradish peroxidase (1/20000; ThermoFisher Scientific Cat# 32230, RRID:AB_1965958). The immunoblot was revealed with SuperSignal™ West Pico PLUS Chemiluminescent Substrate (ThermoFisher Scientific Cat# 34580 ...
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bioRxiv - Developmental Biology 2021Quote: ... 1% L-glutamine, 55nM 2-Mercaptoethanol, 1000 U/mL Leukemia Inhibitory Factor (LIF) (ESGRO, Catalog #ESG1107) in Knockout DMEM (Gibco, Catalog #10829-018)).
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bioRxiv - Bioengineering 2022Quote: ... The sample were then incubated with 1:500 Alexa Fluor 488-conjugated goat anti-Mouse IgG (H+L) secondary antibody (Thermo Fisher Scientific, A11001) in blocking buffer at RT for 45 min ...
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bioRxiv - Microbiology 2022Quote: ... Parasites were attached to poly-L-lysine coated plates and incubated in RPMI containing wheat germ agglutinin-Alexa 350 (WGA-350) (Invitrogen, Catalog no. W7024) at 5μg/ml final concentration and incubated for 12 mins to stain the erythrocyte plasma membrane ...
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bioRxiv - Neuroscience 2021Quote: ... mAb24B10 (DSHB, 1:50), anti-OLLAS (Funakoshi, 1:200) and goat anti-rat IgG(H+L) Alexa Flour 488 conjugate (Life Technologies, 1:400).
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bioRxiv - Neuroscience 2021Quote: ... The following secondary antibodies were used in this study were: goat anti-rabbit IgG (H&L) conjugated with DyLight 488 (Thermo Fisher Scientific, 35553) and goat anti-mouse IgG (H&L ...
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bioRxiv - Molecular Biology 2020Quote: ... The accurate mass of L-lysine from 20-fold diluted samples (supernatants containing L-lysine for quantification) was analyzed using an LC-MS Fusion (Thermo Fisher Scientific, USA) with positive electrospray ionization (ESI+) ...
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bioRxiv - Molecular Biology 2021Quote: ... and their derivatives were cultured in DMEM containing 1 g/l glucose supplemented with 10% heat-inactivated FCS (Fetal Calf Serum, Gibco, Cat#: 10270-106), 2 mM L-glutamine (Thermo Scientific ...
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bioRxiv - Genomics 2019Quote: ... The cells were further incubated for 10 min in Alexa Fluor 488-conjugated donkey anti-sheep LgG (H+L) antibody (ThermoFisher Scientific, A-11015), washed and resuspended in sorting buffer ...
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bioRxiv - Cell Biology 2019Quote: ... All animals were washed off the plates with M9 and transferred to a 2 l Erlenmeyer flask containing 500 ml S-medium supplemented with penicillin-streptomycin (5000 U/ml, Life Technologies 15070-63) diluted 1:100 ...
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bioRxiv - Systems Biology 2019Quote: ... each transformant growing on solid SC-W or SC-L was picked by scratching the corresponding spot and inoculated into a 96-well microplate (Fisher Scientific/Corning, #07200720A) containing 160 μL of liquid SC-W or SC-L per well ...
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bioRxiv - Microbiology 2019Quote: ... cultures were diluted 1:1333 in milliQ water and then adhered on a poly-L-lysine coated glass slide (Thermo Scientific, Menzel-Gläser) for 30 min prior to incubation in pre-warmed RPMI + 10 % FCS at 37°C for 45 min- 3 h 15 min depending on the tested parameter ...
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bioRxiv - Microbiology 2019Quote: ... to generate a 450nt fragment encoding the carboxy-terminus of the nucleoprotein gene (N450).15 First-round RT-PC R amplification was performed after adding 5µl of RNA extract to 15µl SuperScript™ III One-Step RT-PCR System with Platinum™ Taq DNA Polymerase (Invitrogen, Carlsbad, CA) mixes ...
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bioRxiv - Biophysics 2019Quote: Cells were cultured at 37°C in 5% CO2 in DMEM which contains 4.5 g/l of glucose and supplied by fetal bovine serum (Gibco/Life Technologies #16140-071) and 1% Pen Strep (Gibco/Life Technologies#15140-122 ...
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bioRxiv - Cell Biology 2021Quote: ... 10 times and enzymes were inactivated by adding 5 ml of RPMI 1640 medium (w/o L-glutamine and phenol red) supplemented with 1 mM sodium pyruvate (ThermoFisher Scientific, Waltham, MA), 9% Nu-Serum™ IV Growth
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bioRxiv - Cancer Biology 2019Quote: ... Cell lines were cultured in RPMI-1640 media supplemented with 10% heat-inactivated fetal bovine serum (FBS) and 2 mM L-Glutamine (Gibco, Grand Island, NY) and maintained at 37 °C in 5% CO2.
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bioRxiv - Evolutionary Biology 2020Quote: ... and CRFK (CVCL_2426) cells were grown on tissue-culture treated plates in high-glucose and L-glutamine containing DMEM (Thermo Fisher, Waltham, MA, USA) supplemented with 1x penicillin/streptomycin (Thermo Fisher ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were subsequently mounted on a coverslip coated with poly-L-lysine that is submerged with Prolong Diamond mounting media (Thermo Fisher Scientific Inc) for imaging.
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bioRxiv - Neuroscience 2020Quote: ... Microglia were cultured as adherent cells in poly-L-lysin coated T75 tissue culture flasks in microglia culture medium containing DMEM/F12 (Gibco™, Thermo Fisher Scientific), 10% fetal calf serum (Biochrom ...
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bioRxiv - Immunology 2021Quote: ... The cells were again washed three times with PBS and then stained with an Alexa Fluor 594-conjugated goat anti-rabbit IgG (H+L) highly cross-adsorbed secondary antibody (Thermo Fisher Scientific #A11037), diluted in BSA/PBS for 1 h in the dark at room temperature ...
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bioRxiv - Immunology 2021Quote: ... Alexa Fluor 488 goat anti-rabbit IgG (H+L) and Alexa Fluor 488 goat anti-mouse IgG1 (γ1) were purchased from Fisher Scientific (Waltham, MA).
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bioRxiv - Genomics 2021Quote: ... Macrophages were cultured by plating 14*106 PBMC resuspended in 2 ml macrophage media (Macrophage-SFM [with L-Glutamine without Antibiotics], Fisher Scientific UK LTD). After 1h 30’ non adherent cells were removed and 1 ml fresh macrophage media added together with 400 μl of autologous serum ...
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bioRxiv - Molecular Biology 2020Quote: L-AN-5 cells were maintained in RPMI whereas SH-SY5Y and HEK293T cells were maintained in DMEM medium (Gibco, Dublin, Ireland, 32430100). For all three cell lines the cell culture medium was supplemented with 10% fetal bovine serum (Gibco ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells re-suspended at a concentration of 1000 cells/µl were incubated for 30 minutes at 4°C with 1 µl Red LIVE/DEAD Fixable Dead Cell Stain (Thermo Fisher Scientific – L23102). After one wash in PBS ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were then washed three times with PBST and stained with donkey anti-rabbit IgG (H+L) Alexa Fluor Plus 488 (Thermo Fisher Scientific, # A32790) in blocking buffer for 1 hour at room temperature followed by 1 hour of DAPI (Roche ...
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bioRxiv - Biophysics 2021Quote: ... cells were incubated in blocking buffer with 8 ng/μL of custom labeled secondary antibodies or of commercial IgG-gam-F(ab’)2-Alexa Fluor 647 (DOL ~ 3) (Thermo Fisher, A-21237) for 45 min ...
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bioRxiv - Microbiology 2020Quote: The emm12 GAS scarlet fever isolate HKU164 and isogenic derivatives were routinely grown at 37°C on 5% horse blood agar or statically in Todd-Hewitt broth supplemented with 1% yeast extract (THY) or chemically defined medium (CDM; Gibco RPMI 1640 with L-glutamine and phenol red (Life Technologies; 11875-093) supplemented with 0.7% (w/v ...