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Citations for Thermo Fisher :
9151 - 9200 of 10000+ citations for 5 Nitrofluorescein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... the automatic switching of a ten-port valve eluted the trapped mixture on areversed phase column (Biobasic-C18, 0.180 i.d., 100 mm length, 5 μm particle size, Thermo Fisher Scientific) for the separation with an acetonitrile gradient (eluent A ...
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bioRxiv - Immunology 2020Quote: ... cells were washed with ice cold sterile 1x DPBS and incubated for 5 min in 1ml TRIzol (Invitrogen, Leicestershire, UK) at RT ...
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bioRxiv - Cell Biology 2019Quote: ... 10-15% of RIPA-soluble fraction and 2.5-5% of insoluble fraction were loaded on 4-12% Bis-Tris gels and run using MOPS buffer (Life Technologies) and visualized by western blotting on PVDF ...
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bioRxiv - Neuroscience 2019Quote: ... The samples were heated to 95°C for 5 minutes prior to electrophoresis through a 12% Bis-Tris precast gel (Invitrogen), followed by transfer to a nitrocellulose membrane by wet blotting ...
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bioRxiv - Evolutionary Biology 2019Quote: ... the proboscis was inserted into a 20 μL tip containing 5 μL of Fetal Bovine Serum (FBS) (Gibco, MA, USA). After 30 min ...
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bioRxiv - Immunology 2019Quote: ... In EdU-only labelling experiments mice were injected with 1 mg EdU (5-ethynyl-2’-deoxyuridine, cat #E10415, ThermoFisher Scientific) intraperitoneally and were killed one hour after injection ...
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bioRxiv - Biochemistry 2019Quote: ... 2 μg of each antibody was diluted in 30 μl PBS-T and then incubated with 5 μl protein A-coated magnetic beads (30 mg/ml, Dynabeads, 10002D, Invitrogen) for 30 min ...
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bioRxiv - Genomics 2019Quote: ... containing 5 μl nuclease-free water with bovine serum albumin (1 mg/μl, Fermentas) and RNaseOut 20 U (Life Technologies). The plates were placed on a precooled rack and stored at −80 °C until analysis ...
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Inhibition of nucleotide synthesis promotes replicative senescence of human mammary epithelial cellsbioRxiv - Systems Biology 2019Quote: Metabolites were detected and quantified as area under the curve based on retention time and accurate mass (≤ 5 ppm) using the TraceFinder 3.3 (Thermo Scientific) software ...
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bioRxiv - Molecular Biology 2019Quote: Three specific 5’ RACE primers and two 3’ RACE primers were designed according to the Race kit instructions (Invitrogen & Clontech) (Table S1) ...
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bioRxiv - Biochemistry 2020Quote: ... Grids were blotted for 3 seconds at 0 force and 5 seconds wait time before being plunge vitrified in liquid ethane using a MarkIV Vitrobot (ThermoFisher). The blotting chamber was maintained at 22°C and 100% humidity during freezing.
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bioRxiv - Microbiology 2019Quote: ... falciparum cell lines were cultured in 5% human O+ red blood cells (Australian Red Cross blood service) in RPMI-GlutaMAX-HEPES (Invitrogen) supplemented with 5% (v/v ...
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bioRxiv - Molecular Biology 2019Quote: ... were used for studies of INS-1 cells treated with CSE or AC extract with or without 5 mM N-Acetyl Cysteine (NAC) from Invitrogen; Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2019Quote: HEK293 cells (ATCC Cat# CRL-1573) were maintained at 37° C and 5% CO2 in 1X DMEM medium (Thermo Scientific; Waltham ...
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Targeting MOG to skin macrophages prevents EAE in macaques through TGFβ-induced peripheral tolerancebioRxiv - Immunology 2019Quote: Macaque PBMCs (2×106cells/well) were cultured for 44 hr (37°C, 5% CO2) in 500 μl of IMDM (ThermoFisher) supplemented with 10% FCS and 1% penicillin/streptomycin in the presence or absence of 20 μg/ml of rhMOG ...
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bioRxiv - Genetics 2019Quote: ... Lysates were prepared from harvested cells in lysis buffer (50mM Tris pH7.5, 150mM NaCl, 0.5% Triton X-100, 5 mM EDTA) containing phosphatase (Thermo Scientific; 78428) and protease inhibitors (Roche ...
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bioRxiv - Neuroscience 2019Quote: ... 100 U/ml−1 penicillin/streptomycin and 5% (v/v) heat-inactivated fetal bovine serum (all supplements were from Invitrogen). Cells were maintained at 37°C in an atmosphere of 5% CO2 ...
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bioRxiv - Physiology 2020Quote: ... blot dried for 5 seconds and immediately placed in Roswell Park Memorial Institute media - 1640 (RPMI-1640, ThermoFisher Scientific Australia) containing 3% (w:v ...
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bioRxiv - Immunology 2019Quote: ... Aim2 inflammasome activation in LPS-primed J774A.1 cells was induced by transfection of 5 μg of poly(dA:dT) using Lipofectamine 2000 (Thermo Fisher) 6 h prior to sample collection [35] ...
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bioRxiv - Molecular Biology 2019Quote: ... Coverslips were washed with 50% formamide/2xSSC at 37°C and then twice with 2xSSC/0.1% TX100 for 5 minutes before mounting using prolong gold antifade with DAPI (ThermoFisher Scientific).
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bioRxiv - Synthetic Biology 2020Quote: ... A type IIS assembly reaction was set up into a 0.2 mL tube (5 µL nuclease-free H2O, 1 µL 10x Tango Buffer (Thermo Fisher), 0.5 µL mg/mL bovine serum albumin (NEB) ...
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bioRxiv - Cell Biology 2019Quote: ... 5×105 MDCK II cells were seeded into 24 mm transwells one day before transfection using Lipofectamine 3000 Reagent (Invitrogen). 6 h post transfection ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were washed 3 times with PBS (5 min, RT) and mounted using ProLong® Gold antifade mountant (ThermoFisher, P36930). STED microscopy was performed using a 100× objective on the STEDYCON 2-colour STED imaging system (Abberior Instruments) ...
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bioRxiv - Biochemistry 2019Quote: ... 5% [v/v] glycerol) was concentrated to 9 mg/mL (measured by UV absorbance in a NanoDrop spectrophotometer (Thermo Scientific) using the calculated molecular weight and estimated extinction coefficient ...
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bioRxiv - Molecular Biology 2019Quote: ... and the liver samples were taken and immediately submerged in 5 mL of RNA later® solution (Ambion, Courtabeuf, France) to preserve the integrity of RNA ...
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bioRxiv - Systems Biology 2019Quote: ... Cells were cultured in a humidified cell incubator at 37 °C with 5% CO2 conditions and split with TrypLE Express (Gibco, Invitrogen) every 2-3 days.
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Therapy-induced lipid uptake and remodeling underpin ferroptosis hypersensitivity in prostate cancerbioRxiv - Cancer Biology 2020Quote: Lipid peroxidation was measured by live ratiometric fluorescence microscopy of ATT pre-treated cells after incubation in 65 µl/well of serum-free RPMI media (0.2% lipid-free BSA) supplemented with 5 µM with BODIPY 581/591 C11 (Thermo Fisher) for 60 min at 37°C ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were washed three times with PBS for 5 min and incubated during 1 hour at RT with AlexaFluor (Invitrogen) secondary antibodies ...
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bioRxiv - Biophysics 2019Quote: 20-nm fluorescent dark red beads samples (Fig. 2d, Supplementary Fig. 5) were prepared using a 5.10−7 dilution of the initial solution (F8783, Thermo Fisher). We performed the dilution in PBS + 5% glucose to match the index of the dSTORM imaging buffer ...
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bioRxiv - Immunology 2019Quote: ... at 3:1 beads:cell ratio in T cell media (AIMV supplemented with 5% FBS, 10mM HEPES, 2mM GlutaMAX, 100 U/mL penicillin, and 100ug/mL streptomycin (Gibco)) ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were cultured in RPMI 1640 medium containing 10% fetal bovine serum and 2 mM L-glutamine at 37 °C in 5% CO2 and passaged at 70–90% confluency with 0.05% TrypLE Express (all from ThermoFisher Scientific).
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bioRxiv - Cell Biology 2019Quote: ... Protein expression was performed using a 2800 ml baffled shaker flask (Fisherbrand, BBV2800) as follows: The 5 ml of starter culture was added to 500 ml of MagicMedia (K6815, Invitrogen), supplemented with 50 µg/ml kanamycin sulfate and incubated as above for 24 h at 30 °C (for FGF2 or TGFβ3 ...
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bioRxiv - Biochemistry 2019Quote: ... Coverslips were washed three times for 5 min with PBS and incubated with 2 μg/mL goat anti-mouse Alexa Fluor 555 secondary antibody (Invitrogen,), in the dark for 1 h at 37 °C ...
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bioRxiv - Molecular Biology 2019Quote: ... Labeled DNA (500 ng) was precipitated during 30 min incubation at −20°C after adding 5 μl of mouse Cot-1 DNA (Invitrogen), 0.5 volume of 7.5 M ammonium acetate and 2.5 volumes of cold 100% ethanol ...
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bioRxiv - Neuroscience 2019Quote: ... The cultures were recovered in serum-containing media (Neurobasal media supplemented with 10% horse serum, 5% fetal bovine serum, and 2 mM glutamine [Gibco]) for 4 h and maintained in serum-free media (Neurobasal media supplemented with B-27 (1:50 diluted) ...
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bioRxiv - Genetics 2020Quote: ... For morphological analyses 105 cells were resuspended in 200 ml PBS and spun (5 min, 400 rpm) in a Cytospin 4 (ThermoFisher), before staining with modified Wright’s Stain on a Hematek (Bayer Health Care) ...
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bioRxiv - Developmental Biology 2019Quote: ... and mir152 specific primers with NCode miRNA First-Strand cDNA Synthesis qPCR Universal Primer in a fast mode SybrGreen reaction at the QuantStudio 5 (Thermofisher). We used RNU6B and RNU44 as endogenous controls ...
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bioRxiv - Molecular Biology 2019Quote: Huh-7 (human hepatoma) cells were maintained at 37 °C and 5% CO2 in Dulbecco’s Modified Eagle Medium (DMEM, Life Technologies) supplemented with 10% (vol/vol ...
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bioRxiv - Immunology 2019Quote: ... the blood system of the whole body was labelled by injecting 100 µl of WGA-Alexa Fluor 555 (5 mg/ml, Invitrogen) with 10 µl Heparin (100 units/ml ...
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bioRxiv - Biochemistry 2019Quote: ... The supernatant was analyzed by reversed-phase HPLC using a Hypersil GOLD aQ column (5 μm particle size, 150 × 4.6 mm, flow rate 0.5 ml at 52°C, Thermo Fisher Scientific) with a mobile phase of H2O-0.05% trifluoroacetic acid and a 25% acetonitrile gradient over 130 min ...
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bioRxiv - Systems Biology 2019Quote: ... 25 mM Tris-HCl pH 7.4, 150 mM NaCl, 1 mM EDTA, 1% NP-40 and 5% glycerol; Thermo Fisher) containing 1x protease inhibitor cocktail III (Calbiochem) ...
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bioRxiv - Cancer Biology 2020Quote: ... maintained in Dulbecco’s modified Eagle’s Medium (DMEM) with 10% FBS at 37°C and 5% CO2) were transiently transfected using Lipofectamine 2000 (Life Technologies). Retroviral particles were obtained and concentrated by centrifugation of the conditioned media (6000 × g overnight at +4°C) ...
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bioRxiv - Clinical Trials 2019Quote: ... and MDCK-SIAT cells (WHO CC London) were maintained at 37°C with 5% CO2 in Dulbecco’s modified Eagle’s Medium (DMEM) (Gibco-Life technologies) supplemented with 10% foetal bovine serum ...
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bioRxiv - Neuroscience 2019Quote: ... A sequence including the zinc finger binding sequence (5’-GTCATCCTCATC-3’) (Gross et al., 2013; Perez et al., 2008) upstream of the hsyn1 promoter was synthesized (ThermoFisher) and inserted using the MluI and EcoRI restriction sites to generate pAAV-ZFBS-syn-PSD95.FingR-dNES-CaMPARI2_F391W_L398V-CCR5TC ...
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bioRxiv - Plant Biology 2019Quote: ... Every qRT-PCR reaction was carried out in a 10μL volume containing: 5 μL SsoAdvanced Universal SYBR Green Supermix (Applied Biosystems), 1 uL template ...
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bioRxiv - Zoology 2020Quote: ... This extended COI fragment was amplified using the dgLCO1490 (5’-GGT CAA CAA ATC ATA AAG AYA TYG G-3’) and COI-R1 (5’-TGT TGR GGG AAA AAR GTT AAA TT-3’) degenerate primers (synthesized by Invitrogen) from Meyer et al ...
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bioRxiv - Immunology 2020Quote: ... Samples were diluted 1:5 in TE buffer and 6% were mixed with TaqMan Universal PCR Master Mix (Applied Biosystems). The sample mix and TaqMan assays were loaded separately into the wells of 96.96 Gene expression Dynamic Arrays (Fluidigm ...
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bioRxiv - Immunology 2019Quote: ... Migrated LCs were harvested after 48 hours culture of epidermal fragments in full culture media (RPMI, Gibco, UK, 5%FBS, Invitrogen, UK ...
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bioRxiv - Plant Biology 2019Quote: ... The 5’ RACE adapter (Supplementary Table S2) was then ligated to the 5’ ends of the + or –TAP treated RNA with T4 RNA ligase (Ambion). For this ...
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bioRxiv - Molecular Biology 2020Quote: ... 5 µl of 10X TURBO™ DNase Buffer and 1 µl of TURBO™ DNase (Art. No. AM2238, Invitrogen™) were added to the RNA and incubated at 37 °C for 30 minutes with gentle shaking ...