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Citations for Thermo Fisher :
9051 - 9100 of 10000+ citations for 5 Nitrofluorescein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... Individual reactions were heated to 65 °C for 5 min and transferred to ice for 3 min to facilitate annealing in SuperScript III reaction buffer (Invitrogen). After annealing ...
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bioRxiv - Developmental Biology 2021Quote: ... followed by mechanical and enzymatic dissociation by pipetting every 5 minutes in protease solution (0.25% trypsin (Life Technologies, 15090-046), 1 mM EDTA ...
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bioRxiv - Developmental Biology 2021Quote: A gene fragment spanning from 488 base pairs upstream from the 5’UTR through 61 base pairs downstream from exon 1 was cloned into pDONR221 using a BP reaction (ThermoFisher) using the primers prkci-BP-forward 5’-GGGGACAAGTTTGTACAAAAAAGCAGGCTCctatctaggtatatgggccctc-3’ and prkci-BP-reverse 5’-GGGGACCACTTTGTACAAGAAAGCTGGGTcgcaatcctgagaataagtgaga-3’ ...
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bioRxiv - Developmental Biology 2021Quote: ... Alkaline phosphatase staining was performed using the one-step nitro-blue tetrazolium (NBT) and 5-bromo-4-chloro-3’-indolyphosphate p-toluidine salt (BCIP) solution (Thermofisher).
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bioRxiv - Developmental Biology 2021Quote: ... at 5 m/s for 30 s with 1.4 mm ceramic beads (15-340-153, Fisher Scientific, Waltham, MA, USA). HH37 and older lower jaws were homogenized at 5 m/s for 60 s with 2.8 mm ceramic beads (15-340-154 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Institution Code: CBTCCCAS) and were cultured at 37°C in a humidified incubator with 5% CO2/95% air with MEM (Gibco) supplemented with 1.5 mg/ml NaHCO3 ...
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bioRxiv - Microbiology 2021Quote: ... 11 µl of nucleic acids were incubated for 2 min at 72°C with 100 pmol of primer A (5’GTTTCCCACTGGANNNNNNNN3’) followed by a reverse transcription step using 200 units of Superscript III (Invitrogen) at 50°C for 60 min with a subsequent Klenow DNA polymerase step using 5 units (New England Biolabs ...
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bioRxiv - Microbiology 2021Quote: HCT8 cultures infected with 5 × 106 oocysts were for 48h were treated with Trypsin-0.25% EDTA (ThermoFisher Scientific, Waltham, MA), pelleted ...
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bioRxiv - Microbiology 2021Quote: ... Membranes were blocked in TBST with 5% milk protein and probed with the following antibodies: 1:2000 anti-Ty1 (Invitrogen), then 1:1500 goat anti-mouse IgG-HRP (Dako) ...
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bioRxiv - Genomics 2021Quote: ... and the amplified fragments were visualized by electrophoresis on agarose gels (2% for DBP1 and 1% for RBP2a) in 1x TAE buffer (40 mM Tris-acetate, 1 mM EDTA) with 5 μg/ mL ethidium bromide (Invitrogen) in a horizontal system (Bio-Rad ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 5 kb of the 5’ flanking region of MpBLD10 with a SmaI site was introduced into the pENTR/D-TOPO vector (Invitrogen). The chimeric sequence was introduced into pMpGWB307 (Ishizaki et al ...
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bioRxiv - Evolutionary Biology 2021Quote: ... freshly cultured hESC colonies were dissociated into clumps (2-5 cells) with Versene buffer (with EDTA, Gibco, Cat. No. 15040066). The dissociated cells were seeded on Geltrex-coated 6-well tissue culture plates at 25,000 cells/cm2 (0.25M per well in the 6-well plates ...
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bioRxiv - Developmental Biology 2021Quote: ... 5 ng of isolated total RNA was used as a template for the RT performed using SuperScript IV (Invitrogen, 18090050), according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... for 5 min at room temperature with gentle agitation followed by mounting samples with ProLong Gold antifade reagent with DAPI (Invitrogen). Images were collected with an Olympus FluoView-1000 confocal microscope and processed using Olympus FluoView Ver.2.0a Viewer software at University of California ...
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bioRxiv - Developmental Biology 2020Quote: ... samples were indexed by ligating TruSeq Illumina adaptors by incubating DNA-bound beads in 50 μl adapter ligation mix (1x T4 Ligation buffer, 5% PEG-4000, 0.3 U/ μl T4 DNA Ligase (ThermoFisher, EL0011), 1.5 μl TruSeq index adapter) ...
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bioRxiv - Developmental Biology 2020Quote: ... and cultured at 37 °C in a humidified 5% CO2 atmosphere with kidney culture medium (F12:DMEM (1:1) + Glutamax (Gibco) supplemented with 10% fetal bovine serum and penicillin-streptomycin)(Ihermann-Hella & Kuure ...
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bioRxiv - Developmental Biology 2020Quote: ... at 5 m/s for 30 s with 1.4 mm ceramic beads (15-340-153, Fisher Scientific, Waltham, MA, USA). HH37 and older lower jaws were homogenized at 5 m/s for 60 s with 2.8 mm ceramic beads (15-340-154 ...
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bioRxiv - Genomics 2020Quote: ... The HEK293T cells were seeded in a 96-well plate at a cell density of 1.5 x 104 and transfected the next day with 100 ng of mini-genes using Lipofectamine 3000 transfection reagent (Invitrogen), in 4 biological replicates ...
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bioRxiv - Genomics 2020Quote: ... the DNA from above was incubated with 1 μl (5 U/μl) of single-strand specific S1 nuclease (ThermoFisher Scientific) in 1x reaction buffer (40 mM sodium acetate ...
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bioRxiv - Bioengineering 2022Quote: ... DAPI was used as a counterstain at 300nM in 1xPBS for 5 minutes at room temperature and then mounted in mounting medium ProLong Diamond Antifade (ThermoFisher). Slides were imaged on a Keyence BZ-X810 microscope using the 10X objective ...
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bioRxiv - Neuroscience 2022Quote: ... and maintained in culture in Ham’s F-12 culture medium (PAA Laboratories) with 5% foetal bovine serum (Thermo Fisher Scientific). Cell culture media containing sNEGR1 secreted by the cells was collected and centrifuged for 15 min at 5000 g at 4°C to remove cell debris ...
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bioRxiv - Immunology 2022Quote: HEK293-hACE2 cells15 or VeroE6-TMPRSS214 were cultured in DMEM with 10% FBS (Hyclone) and 1% PenStrep and 8ug/mL puromycin for TMPRSS2 maintenance with 5% CO2 in a 37°C incubator (ThermoFisher). One day prior to infection ...
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bioRxiv - Immunology 2022Quote: ... The cells were individually collected using an automated micro-manipulating system (CellCelector, ALS) and deposited in nuclease-free microtubes containing 5 μL of 2× CellsDirect buffer and RNase Inhibitor (Invitrogen). Single cell RT-qPCR was then performed using the protocol ADP41 developed by Fluidigm ...
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bioRxiv - Cancer Biology 2022Quote: ... cDNA was synthesized from 5 ng of total RNA using TaqMan® Small RNA Assays RT-primers (Thermo Fisher Scientific) with the High-Capacity cDNA Reverse Transcription Kit ...
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bioRxiv - Cell Biology 2022Quote: ... 1 μg of plasmid was used to transfect 750,000 cells in each well of a 6-well plate using Lipofectamine 2000 (5 μL; Thermo Fisher) in OptiMEM (400 μL ...
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bioRxiv - Immunology 2022Quote: ... HEK293 cells stably transfected with human CXCR4 (HEK293- CXCR4) were used at passage 5 and were cultivated in DMEM medium (Gibco), supplemented with 10% FCS and 1% penicillin/streptomycin (Gibco) ...
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bioRxiv - Immunology 2022Quote: Annealed oligonucleotides containing the sequence of the HCV 5’ppp poly-U/UC region (34) were in vitro transcribed using the MEGAshortscript T7 transcription kit (Ambion) followed by ethanol precipitation ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... XLK-WG cells were cultured as directed by the supplier at 29°C/5% CO2 in RPMI-1640 medium supplemented with 20% fetal bovine serum (FBS; ThermoFisher) and passaged at ∼70% confluence using a 0.25% trypsin EDTA solution in 1X PBS ...
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bioRxiv - Genomics 2022Quote: ... as a monolayer at 37°C in a humidified atmosphere with 5% CO2 using Iscove’s Modified Dulbecco’s Medium (IMDM) (Life Technologies, CA) supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Cancer Biology 2022Quote: ... Cellular membranes were labeled with 5 μg/ml wheat germ agglutinin (WGA) conjugated to Alexa Fluor 488 (Thermo Fisher Scientific) for 10 min at room temperature ...
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bioRxiv - Physiology 2022Quote: ... Coverslips were subsequently washed in quadruplicate (5 min each) with PBS and diluted secondary antibody (1:500 goat anti-rat IgG Alexa Fluor 488 conjugate, Invitrogen) was applied as above ...
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bioRxiv - Cell Biology 2022Quote: ... Infection was performed in the 24-well plates and cells were subsequently selected for 5 days with Geneticin (ThermoFisher Scientific) at 75 μg/mL ...
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bioRxiv - Microbiology 2022Quote: THP-1 monocytes from the Duke Cell Culture Facility were cultured at 37°C in 5% CO2 in RPMI 1650 media (Invitrogen) supplemented with 10% heat-inactivated FBS ...
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bioRxiv - Neuroscience 2022Quote: ... Recordings were made with patch pipettes (3-5 MΩ) containing aCSF and 10 µM alexa-594 (Thermofisher, Waltham, MA, USA) and CSFcNs were targeted under visual guidance using their fluorescence ...
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bioRxiv - Neuroscience 2021Quote: ... We washed the cells 3 times for 5 minutes each with 1X PBS and mounted with ProLong Gold Antifade Mountant with DAPI media (Invitrogen) to preserve the cells for imaging ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were rinsed in Milli-Q water for 3 × 5 minutes and cover slipped with ProLong Gold Antifade (Invitrogen, P36930).
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bioRxiv - Neuroscience 2021Quote: NIH-3T3 cells were seeded on 6-well plates and allowed to grow to 70% confluency at 37°C and 5% CO2 in DMEM (Gibco) supplemented with 10% FBS (Life Technologies) ...
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bioRxiv - Neuroscience 2021Quote: ... Another 4x 15 min wash with PBST was followed by a 5 min incubation with Hoechst 33342 (Thermo Fisher Scientific), a 10 min wash with PBS and a 5 min wash with distilled water ...
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bioRxiv - Neuroscience 2021Quote: ... Brains were then washed 4X in PBS with .5% Triton X-100 (PBS-T) and then placed in blocking solution (PBS-T with 5% normal goat serum (NGS; Invitrogen)) for 90 minutes at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were washed three times with 1X PBS for 5 minutes each and blocked for 30 minutes at RT with Blocking Buffer [0.4% acetylated BSA (Ambion, AM2614) in 4X SSC] in a humidified chamber ...
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bioRxiv - Molecular Biology 2021Quote: ... was added to the cells in half culturing volume (40 uL added) and incubated in the dark for 5 min prior to reading on a Varioskan LUX plate reader (ThermoFisher). Measurements were done on all mAbs and human serum samples from each group in at least duplicate ...
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bioRxiv - Molecular Biology 2020Quote: ... One million live cells were plated in 5 ml complete Neurobasal culture medium (Neurobasal™ Medium (Gibco, cat. no. 21103049), 1x B-27 supplement (Gibco ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then washed three times for 5 mins each and incubated with fluorescently-labeled secondary antibodies (Invitrogen, Carlsbad, CA) in blocking solution for 1 h at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... Tet-on 3’ UTR HP and 5’ UTR HP DG NSCs were brought in suspension by incubating with 0.25% trypsin (Gibco #15090) in Versene (Gibco #15040 ...
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bioRxiv - Molecular Biology 2020Quote: ... and incubated with Super Signal Chemiluminescent Substrate (Thermo 34580) for 5 min with gentle rotation and imaged by iBright FL1000 (Invitrogen).
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bioRxiv - Molecular Biology 2020Quote: ... produced from DKO piglet were prepared and stained at 4°C for 45 minutes with Alexa 488-conjugated Griffonia simplicfolia isolectin IB4 (GS-IB4) (5 μg/mL, Invitrogen). The stained cells were washed twice and then analyzed by flow cytometer (BD Accuri™ C6 Plus) ...
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bioRxiv - Molecular Biology 2020Quote: ... tissue sections were deparaffinized and rehydrated before being digested with 200 μl of 5 μg/ml Proteinase K (Invitrogen, USA) in TNE buffer for 1 h at 37°C ...
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bioRxiv - Molecular Biology 2020Quote: ... 5’TYE563-labeled locked nucleic acid probes (Exiqon) against mouse major satellite sequences (Supplementary Table 5) were precipitated with mouse Cot-1 DNA (Invitrogen) and Salmon Sperm DNA (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples were boiled at 95°C for 5 min before running on a NuPAGE 4-12% Bis-Tris protein gradient mini gel (Invitrogen) at 200 V for 5 min in MOPS running buffer (Invitrogen ...
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bioRxiv - Molecular Biology 2021Quote: ... acetonitrile/water (95/5) with 10 mM ammonium acetate (pH 8.2) (All solvents were LC–MS Optima grade from Fisher Scientific). The total run time was 11 min ...