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Citations for Thermo Fisher :
9001 - 9050 of 10000+ citations for 5 Nitrofluorescein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... The cell suspension was centrifuged at 230 g for 5 min and resuspended in Live Imaging Solution (Thermo Fisher). FerroOrange staining solution of 1 μmol/L was prepared following the manufacturer’s guidelines ...
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bioRxiv - Microbiology 2024Quote: ... between 5 and 50 μL of cell sample was dropped onto either poly-l-lysine coated chambered slides (Nunc Lab-Tek 8-Well chambered slides ...
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bioRxiv - Genomics 2024Quote: ... 5 x 106 HEK 293T cells were seeded in 10-cm dishes in Dulbecco’s minimal essential medium (DMEM) (Gibco) supplemented with 10 % fetal calf serum (FCS ...
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Recruitment of the m6A/Am demethylase FTO to target RNAs by the telomeric zinc finger protein ZBTB48bioRxiv - Molecular Biology 2024Quote: ... RNA-protein complexes were immunoprecipitated using 5 μg antibody (either anti-ZBTB48, or anti-GFP antibody (Life Technologies G10362), or anti-FLAG ...
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bioRxiv - Microbiology 2020Quote: ... labeled RNAs were cooled for 5 min on ice and 1 μg yeast RNA and 10x RNA structure buffer (Ambion) were added ...
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bioRxiv - Microbiology 2020Quote: ... at which point the aqueous layer was removed and transferred to a new tube containing 100 μl of 5 M ammonium acetate (Ambion), 20 μl of 5 mg/ml glycogen (Ambion) ...
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bioRxiv - Synthetic Biology 2021Quote: ... and re-suspended at a density of 104 cells/mL in pre-warmed complete minimum essential media (MEM) that contains 5 mg/mL human recombinant insulin (Gibco), 1X MEM non-essential amino acids (NEAA ...
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bioRxiv - Biophysics 2021Quote: ... cells were grown in an incubation chamber providing 37 °C and 5% CO2 in growth medium (DMEM (catalog no. 11880-02, Gibco)) containing 1× MEM NEAA (catalog no ...
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bioRxiv - Biophysics 2021Quote: ... followed by 1 h incubation with 1:1000 anti-mouse Alexa Fluor 488 conjugated secondary antibody (diluted with PBS containing 5% BSA, Invitrogen) at RT in the dark ...
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bioRxiv - Cancer Biology 2021Quote: ... COS-1 cells were transiently transfected with either 5 or 10 μg of each of pCIneo-FOXA1-V5 and pCIneo-V5 plasmids using lipofectamine 3000 Reagent (Invitrogen).
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bioRxiv - Cancer Biology 2021Quote: ... They were maintained in RPMI 1640 medium supplemented with 5% fetal bovine serum (FBS, Gibco® Life Technologies, #10270-106). Human embryonic kidney (HEK ...
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bioRxiv - Cancer Biology 2021Quote: ... They were maintained in RPMI 1640 medium supplemented with 5% fetal bovine serum (FBS, Gibco® Life Technologies, #10270-106). Human embryonic kidney (HEK ...
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bioRxiv - Neuroscience 2021Quote: ... and 20 µg protein lysate was denatured at 95°C for 5 min and then separated on a 10-20% Tris-tricine gel (Invitrogen) at 125V for 90 min ...
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bioRxiv - Cancer Biology 2021Quote: ... Tissue sections were blocked with 3% bovine serum albumin (BSA, Fisher Bioreagents) and 5% normal goat serum (NGS, Gibco #PCN5000) in PBS for 1 hour at room temperature ...
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bioRxiv - Biophysics 2019Quote: ... the sample was heated to 95 °C for 5 min and 15 µl of sample were resolved by a 12% NuPAGE Bis-Tris gel (Invitrogen). Proteins were transferred onto a Immobilon-P polyvinylidene difluoride membrane (Millipore ...
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bioRxiv - Biophysics 2019Quote: ... the sample was heated to 95 °C for 5 min and 15 µl of the sample were resolved by 12% NuPAGE Bis-Tris gel (Invitrogen). Molecular weight markers were purchased from ThermoFisher Scientific (PageRuler™ Plus Prestained Protein Ladder ...
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bioRxiv - Biophysics 2020Quote: ... at 37 °C in a 5% (v/v) CO2 environment (Forma Steri-Cycle CO2 incubator, Thermo Fisher Scientific, Massachusetts, USA).
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bioRxiv - Cancer Biology 2021Quote: ... Peptides were trapped at 20 μL/min in loading solvent (2% acetonitrile, 0.05% TFA) on a 5 mm × 300 μm C18 PepMap cartridge pre-column (Thermo Fisher Scientific / Dionex Softron GmbH ...
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bioRxiv - Cancer Biology 2021Quote: ... Organoid cultures were expanded in growth medium without addition of Wnt3A-conditioned medium (serum-free condition) for 5 days and dissociated into single cells for 30-60 min using AccuMax (Invitrogen) supplemented with 0.3 mg/ml Dnase I ...
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bioRxiv - Systems Biology 2020Quote: ... fixed and blocked HeLa CCL-2 cells were incubated with 5 μg/mL rabbit anti-Ki67 (Thermo Fisher Scientific; RB1510P1ABX) in 1X blocking buffer for 45 min ...
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bioRxiv - Systems Biology 2020Quote: ... fixed and blocked HeLa CCL-2 cells were incubated with 5 μg/mL rabbit anti-Ki67 (Thermo Fisher Scientific; RB1510P1ABX) for 45 min in 1X blocking buffer ...
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bioRxiv - Cell Biology 2020Quote: 20*106 THP-1 cells were treated with doxycycline (200 ng/ml) for 5 h and stimulated with L18-MDP (200 ng/ml, Invitrogen) for 30 min ...
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bioRxiv - Cell Biology 2020Quote: ... and HeLa cell lines were cultured at 37°C and 5% CO2 in DMEM (Dulbecco’s Modified Eagle Medium) containing high glucose (4.5 g/L; Thermo Fisher Scientific) with 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2020Quote: Whole protein extracts were prepared from JJN3 pellets (4°C, 150 x g, 5 min) according to the cell extraction protocol for ELISA sample preparation by ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... A Lsm12-KO cell line of HEK293 cells was generated with Synthego’s chemically modified sgRNA 5’-CCAGAAUGUCCCUCUUCCAG-3’ and GeneArt Platinium Cas9 nuclease (ThermoFisher Scientific) that were transfected together into cells using Lipofectamine CRISPRMAX Cas9 transfection reagent (ThermoFisher Scientific).
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bioRxiv - Cell Biology 2020Quote: We seeded 2×105 cells / well into 6 well dishes and replaced media with 5% KSR (Knockout Serum Replacement) (Gibco) containing media the next day ...
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bioRxiv - Cell Biology 2020Quote: ... 20*106 THP-1 cells were treated with doxycycline (200 ng/ml) for 5 h and stimulated with L18-MDP (200 ng/ml, Invitrogen) for 30 min ...
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bioRxiv - Cell Biology 2020Quote: ... collected into a new tube and stained with 4′,6-diamidino-2-phenylindole (DAPI) (∼5 µg/ml, Molecular Probes # D1306). Round coverslips (Ø12 mm ...
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bioRxiv - Cell Biology 2020Quote: ... 5 μm particle size) and an analytical column (PepMap C18, 500 × 0.075 μm, 2 μm particle size, Thermo Fisher Scientific), coupled to a Linear Trap Quadrupole Orbitrap Velos (with CID ...
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bioRxiv - Cell Biology 2020Quote: ... RSLC systems were equipped with C18 μ-precolumns (PepMap™, 5 mm x 0.3 mm; Thermo Fisher Scientific, Bremen, Germany) and C18 reversed-phase nano RSLC columns (Acclaim PepMap™ ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were washed three times with PBS 0.1% Triton X-100 for 5 minutes and nuclei were counterstained with 2 μg/μl Hoechst 33342 (Invitrogen H3570) in PBS 0.1% Triton X-100 for 5 minutes at RT ...
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bioRxiv - Cell Biology 2020Quote: ... sections / cells were then incubated in PBS−/− containing either DAPI (5 μg ml−1; 10 min; RT; Thermo Fisher Scientific) or DRAQ5 (1:500 ...
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bioRxiv - Cell Biology 2020Quote: 1 mg of each of the two pX335 guide RNA/SpCas9n constructs and 5 mg of the Citrine donor template were transfected into 1 million OP9 cells using Lipofectamine 2000 (Invitrogen) following the manufacturer’s protocol.
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bioRxiv - Cell Biology 2020Quote: ... cells were rinsed 4x for 5 minutes in PBS and mounted on glass slides using ProLong Gold antifade reagent (Invitrogen).
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bioRxiv - Cell Biology 2020Quote: ... samples were washed thrice with PBS having 0.3% Triton X-100 (PBTX) and blocked using 5% Fetal Bovine Serum (ThermoFisher Scientific) in PBTX for 2 hrs at RT ...
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bioRxiv - Immunology 2021Quote: OT-I T cells were isolated from spleens of CD45.1 mice and intraveneously injected (1×107 cells) either unlabeled or following CFSE labeling (5 µM, Molecular Probes) into recipient CD45.2 DC ICAM-1 KO mice and their control counterparts ...
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bioRxiv - Immunology 2021Quote: Thymi from 4-week-old wild type mice were harvested and 5 million thymocytes per condition were cultured in IMDM medium (Gibco) with 2% FBS (HyClone Thermo Fischer Scientific ...
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bioRxiv - Immunology 2021Quote: ... monocyte-derived DCs that were transfected with hACE2 were seeded at 0.5×106 cells/mL in a 6-well plate and transfection was performed with Lipofectamine LTX and PLUS reagents (Invitrogen) according to the manufacturer’s instructions for primary cells ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed 3 times with washing buffer (1x PBS and 0.025% of Tween20) for 5 min and incubated for 1h at RT with the Alexa Fluor secondary antibodies (Thermo Scientific). Cells were washed twice with washing buffer for 5 min and incubated in with DAPI (1 μg/mL ...
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bioRxiv - Genetics 2021Quote: The SNP STARRseq library (100ug plasmid DNA/replica) was transfected into LNCaP cells (5 × 107 cells/replica; 3 biological replicas) using the Neon Transfection System (Invitrogen). Cells were grown in RPMI 1640 medium supplemented with 10% FBS and collected 48hrs post-electroporation ...
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bioRxiv - Genetics 2021Quote: ... designing primers to full length MC1R and cloning PCR product of cDNA and genomic from NK cells from 5 heterozygous individuals heterozygous using TA Cloning kit (Thermofisher). In addition ...
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bioRxiv - Genetics 2021Quote: ... cDNA was synthetized from 5 μg of RNA with the Maxima H Minus First Strand cDNA Synthesis Kit (Thermo Scientific) and oligo (dT)18 primer ...
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bioRxiv - Physiology 2020Quote: ... and 5 μl were subjected to high pressure liquid chromatography (HPLC) in a 25 cm PepMap RSLC C18 column (Thermofisher) coupled to tandem mass spectrometry (MS/MS ...
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bioRxiv - Developmental Biology 2021Quote: ... 5 ng cDNA was subjected to real time qPCR using PowerUp™ SYBR® Green Mastermix (A25741, Thermo Fisher Scientific) on the QuantStudio 3 Real-Time PCR system (A28567 ...
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bioRxiv - Immunology 2021Quote: Splenocytes were processed as described in the previous section and stimulated with RBD peptides for 5 hours at 37°C with protein transport inhibitor (Invitrogen) and anti-mouse CD107a-FITC antibody (BioLegend) ...
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bioRxiv - Biochemistry 2020Quote: Protein samples were heated at 95°C for 5 minutes before loading and separated onto SDS-PAGE cassette (Thermo Fisher Scientific Bolt™ 4-12% Bis-Tris Plus Gels ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were washed three times for 5 min in PBT and incubated in Alexa Fluor 488 or 594 secondary antibodies 1:500 (Invitrogen) for 72 h at 4°C ...
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bioRxiv - Microbiology 2019Quote: ... 1 μg of this plasmid was transfected into 5×105 SH-SY5Y cells with Lipofectamine LTX PLUS (1:1 ratio; Invitrogen) and selected for colonies with G418 (0.5 mg/ml) ...
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bioRxiv - Microbiology 2019Quote: ... The alkaline hydrolysis ladder was generated by incubating the end-labeled transcript at 95 °C for 5 min in alkaline buffer (Ambion). RNase T1 was used for 5 min at 37 °C to generate the G ladder ...
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bioRxiv - Microbiology 2019Quote: ... 0.1 pmol of 5’-end labeled manY and thrS mRNAs were incubated at 37 °C for 30 minutes in structure buffer (Ambion) containing 1 ng of yeast RNA (Ambion) ...