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Citations for Thermo Fisher :
9051 - 9100 of 10000+ citations for rno mir 134 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... 500 ng of total RNA was reversed transcribed with the High-Capacity cDNA reverse transcription (RT) kit (Invitrogen) in accordance with the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... An on-column DNase digestion step (PureLink® DNase mixture, Thermo Fisher Scientific, for 20 min at RT) was included to remove genomic DNA (Roche Molecular Systems ...
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bioRxiv - Neuroscience 2024Quote: ... RT) and incubating it for 15 min at 37°C with 0.125% trypsin (# 25200-072, Thermo Fisher Scientific) and 25 U/ml benzonase (# E1014-25KU ...
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bioRxiv - Cancer Biology 2024Quote: ... and aspirates were dried at RT overnight and resuspended in 10 µL of UltraPure water (Invitrogen 10977-023). Each aspirate was then uniquely indexed using the Illumina i5×i7 dual indexing system through Nanostring NGS library preparation kits (Nanostring ...
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bioRxiv - Physiology 2024Quote: ... Reverse-transcription was performed with 850 ng of RNA using the High Capacity cDNA RT kit (Applied Biosystems). Gene expression analysis was carried out using the Wafergen Smartchip cycler and Smartchip Multisample Nanodispenser (Biogenouest Genomics and the EcogenO core facility ...
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bioRxiv - Microbiology 2024Quote: ... and 2 U/µl of M-MulV-RT (Reverse transcriptase of Moloney Murine Leukemia Virus; Thermo Scientific, USA).
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bioRxiv - Molecular Biology 2024Quote: ... RT with rotation) with endogenous CHD4 that was immobilized on 25 µL antibody-conjugated protein G dynabeads (Invitrogen) upon immunoprecipitation from whole cell lysates ...
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bioRxiv - Molecular Biology 2024Quote: Reverse transcription was performed with a Maxima First Strand cDNA Synthesis Kit for RT-qPCR (Thermo Fisher Scientific) using random hexamers ...
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bioRxiv - Microbiology 2024Quote: ... the collected blood samples were centrifuged at 2,000×g for 10 minutes (Thermo Fisher Sorvall Legend RT Centrifuge) to obtain the serum ...
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bioRxiv - Genomics 2024Quote: ... which included 5 µL of 4x TaqPathTM 1-step RT-qPCR Master Mix (Thermo Fisher Scientific, MD, USA), 1 µL of 10 µM forward primer ...
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bioRxiv - Microbiology 2024Quote: ... cells were incubated for 1 h at RT with Alexa Fluor-594 conjugated goat anti-mouse IgG (Invitrogen) diluted 1/1,000 in PBS added with 3% (w/v ...
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bioRxiv - Cell Biology 2023Quote: ... Genomic DNA removal and reverse transcription (RT) were performed using Verso cDNA Synthesis Kit (Thermo Fisher Scientific #AB1453A). Quantitative reverse transcription polymerase chain reaction (qRT-PCR ...
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bioRxiv - Genomics 2024Quote: The reverse transcription solution was prepared with the following composition: 1X RevertAid RT buffer (Thermo Fisher Scientific EP0452), 250 µM dNTPs (New England Biolabs N0447L) ...
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bioRxiv - Immunology 2024Quote: ... Real time quantitative polymerase chain reaction (RT-qPCR) was performed using SYBR Green (Applied Biosystems, San Francisco, CA) were used to assess TNF expression with the following forward and reverse primers (forward ...
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bioRxiv - Molecular Biology 2023Quote: ... mtDNA copy number was determined via RT-qPCR using a probe for Mitochondrial Cytochrome b (Fisher Scientific - Mm04225271_g1). Nuclear DNA copy number was determined using a probe for Ubiquitin C (Fisher Scientific - Mm02525934_g1) ...
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bioRxiv - Cell Biology 2024Quote: ... 2 µg of RNA was initially heated up at 70°C with RT buffer (Thermo Scientific; Cat #EP0441), 0.5 mM dNTP and 0.05 mM Oligo dT18 for 10 min ...
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bioRxiv - Cell Biology 2024Quote: ... A real-time search-synchronous precursor selection-MS3 (RTS-SPS-MS3) method7 was constructed in XCalibur (ThermoFisher Scientific) with the following parameters and then used ...
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bioRxiv - Molecular Biology 2023Quote: ... for 40 minutes at RT before reaction was quenched on ice with 200 U of SuperaseIN (ThermoFisher Scientific). Digested lysate was then analyzed on a sucrose gradient as described above.
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bioRxiv - Microbiology 2023Quote: ... RT-qPCR was performed on 50 ng of cDNA using the SYBRTM Select Master Mix kit (Applied Biosystems) and 250 nM of each primer ...
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bioRxiv - Molecular Biology 2022Quote: RT-qPCR experiments on ES cells and blastocysts were performed using Platinum SYBR Green qPCR SuperMix-UDG (Invitrogen) in LightCycler 480 Instrument (Roche ...
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bioRxiv - Neuroscience 2023Quote: ... RT-qPCR was performed by running the following thermocycler program on a QuantStudio 6 Flex system (Applied Biosystems): 50C for 15 min ...
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bioRxiv - Physiology 2023Quote: ... sections were incubated 2 h at RT with A568 donkey anti-rabbit secondary antibody (1:500; Invitrogen; A10042) in [PB 0.2% Tx + 1% donkey serum] ...
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bioRxiv - Microbiology 2023Quote: ... The cDNA was amplified by RT-qPCR using Fast SYBR Green master mix (Thermo Fisher scientific, Waltham, MA) in a 7500 Fast Real-time PCR system (Applied Biosystems ...
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bioRxiv - Genomics 2023Quote: Reverse transcription (RT) was carried out using the high-capacity cDNA archive kit (Applied Biosystems, Foster City, CA) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... SAT2 (Hs00374138_g1) or ADGRE1 (Hs00892590_m1) were investigated using RT-qPCR and TaqMan Gene Expression assays (Thermo Fisher Scientific). The TBP (Hs99999910_m1 ...
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bioRxiv - Cancer Biology 2023Quote: ... was performed using 4.5µL of 5-fold diluted RT product and 5.5µL of reaction mix (composed of 5µL of TaqMan Universal Master Mix (Applied Biosystems, Life Technologies ...
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bioRxiv - Cell Biology 2023Quote: ... followed by equilibration in 5mM MgCl2/PBS at RT and incubation in 1X SSC [20X SSC (Invitrogen AM9763) diluted in Nuclease free water (AmericanBio ...
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bioRxiv - Immunology 2023Quote: ... Reverse transcription of isolated RNA was performed using the High-Capacity cDNA RT Kit (Thermo Fisher Scientific, 4368814). Amplification reactions (triplicates ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: The tumor slides were air-dried without dehydration for 30’ at RT and washed with 1X PBS (Gibco, Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... and blocked for 1 hour at RT in 3% BSA (Applichem) in PBS-Tween buffer (0.01%) (ThermoFisher, Sigma). The membranes were then incubated overnight at 4°C with primary antibodies listed in the Supplemental table 3 with agitation ...
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bioRxiv - Cell Biology 2023Quote: ... incubated at room temperature (RT) for 45 min on a shaker followed by 3x washing with PBS (Gibco). Cells were permeabilised and blocked with 0.3% Triton X100 (Sigma ...
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bioRxiv - Neuroscience 2023Quote: ... 500ng of the extracted RNA was used for reverse transcription (High-Capacity cDNA RT kit; Life Technologies; 4368814). Transcribed cDNA was diluted ten-fold and used for real-time quantitative PCR (QuantiTect SYBR Green PCR Kit ...
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bioRxiv - Microbiology 2023Quote: ... 100 ng RNA were used as template for the TaqPath 1-Step RT-qPCR assay (Applied Biosystems, A15299), following manufacturer’s instructions and specific ...
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bioRxiv - Microbiology 2023Quote: ... mRNA was converted to cDNA using the Maxima First Strand cDNA Synthesis Kit for RT-qPCR (Fisher Scientific), using random hexamers for first-strand synthesis and equal amounts of RNA (20–30 ng/reaction) ...
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bioRxiv - Cancer Biology 2023Quote: ... Reverse transcription (RT) was performed with the RevertAid First Strand cDNA Synthesis Kit (Life Technologies, Carlsbad, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... DDIT3 and DNAJB1 were evaluated by RT-qPCR using an ABI7500 instrument (Applied Biosystems, Foster City, United States). Total RNA was extracted from mice lung tissue (100 mg ...
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bioRxiv - Genomics 2024Quote: ... using a prepared mix of Maxima H Minus RT Buffer and Reverse Transcriptase 200U/µl (Cat#: EP0752, ThermoFisher), RiboLock RNAse inhibitor 40U/µl (Cat# ...
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bioRxiv - Cell Biology 2024Quote: ... and aliquots (1 μg) of the RNA were subjected to RT with SuperScript IV polymerase (Thermo Fisher Scientific) or PrimeScript RTase (TaKaRa) ...
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bioRxiv - Developmental Biology 2024Quote: ... All culture vessels were pre-coated for 45 minutes at RT with 100 µg/ul of Fibronectin (Invitrogen) to allow explant attachment ...
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bioRxiv - Microbiology 2024Quote: ... The resulting RNA from selective depletion was utilized for random-primed cDNA synthesis with SuperScript IV RT (Invitrogen). Second-strand cDNA was generated using Escherichia coli DNA ligase ...
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bioRxiv - Biochemistry 2024Quote: ... We converted 1 µg of purified RNA to cDNA using a SuperScript IV VILO RT kit (Invitrogen, 11766050). Since apicoplast genes are extremely AT-rich76 and mRNA transcripts are not poly-adenylated or poly-uridylylated80 ...
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bioRxiv - Neuroscience 2024Quote: ... Then the membrane was incubated for 1h at RT with appropriate horseradish peroxidase (HRP)-conjugated secondary antibodies (Invitrogen). The visualization of the signal was conducted using Immobilon Forte Western HRP substrate (Millipore ...
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bioRxiv - Microbiology 2024Quote: ... for 1 hour at RT and signal was developed using SuperSignal West Pico Plus chemiluminescent substrate (Life Technologies) and imaged on a BioRad ChemiDoc.
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bioRxiv - Molecular Biology 2024Quote: ... 0.1% RNAse-free Bovine Serum Albumin for 30 minutes at RT before being incubated with primary antibodies (Thermofisher, mouse anti-tubulin ...
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bioRxiv - Immunology 2024Quote: ... RT-qPCR was then performed using PowerTrack™ SYBR Green Master Mix for qPCR (#A46109, Thermo Fisher Scientific) using specific forward and reverse primers listed in Table EV1 in a 7300 Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Microbiology 2024Quote: ... mRNA was converted to cDNA using the Maxima First Strand cDNA Synthesis Kit for RT-qPCR (Fisher Scientific), using random hexamers for first-strand synthesis and equal amounts of RNA (20–30 ng/reaction) ...
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bioRxiv - Molecular Biology 2024Quote: ... and incubated for 1h at RT with secondary antibodies (Alexa Fluor conjugated produced in goat by Thermo Fisher) diluted in the blocking solution ...
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bioRxiv - Molecular Biology 2024Quote: ... mRNA was converted to cDNA using Maxima First Strand cDNA Synthesis Kit for RT-qPCR (Thermo Fisher, K1671).
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bioRxiv - Immunology 2024Quote: ... cells were stained with Annexin V for 15 mins at RT in 1x binding buffer (Thermo Fisher Scientific), and fresh propidium iodide (PI ...
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bioRxiv - Plant Biology 2024Quote: Reverse transcription was carried out from 1 μg of purified RNA using the RevertAid RT kit (Thermo Scientific) with oligo(dT)18 primer ...