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Citations for Thermo Fisher :
8851 - 8900 of 10000+ citations for rno mir 134 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... Real-Time PCR was performed for each target and sample in triplicate on a StepOnePlus PCR System (Applied Biosystems). All data was analyzed using the Comparative ΔΔCT method to generate relative expression data using rps29 as the internal loading control ...
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bioRxiv - Cancer Biology 2021Quote: ... SYBR Green-based real-time quantitative PCR was performed in the StepOnePlus™ Real-Time PCR System (Applied Biosystems). Primers used in this study are listed in Supplementary Table 5.
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bioRxiv - Cell Biology 2021Quote: ... PCR reactions were performed on equal amounts of cDNA in a with SYBR Green PCR Master Mix (Applied Biosystems). PCR products were run on 1% agarose gels with the GeneRuler 1kb Plus DNA Ladder (ThermoFisher).
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bioRxiv - Cell Biology 2021Quote: ... Quantification of gene expression by q-PCR was performed using Power SYBR Green PCR Master Mix (Applied Biosystems #4309155). Technical triplicates for a minimum of three biological replicates were used ...
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bioRxiv - Cell Biology 2021Quote: ... qRT-PCR analysis was performed using the SYBR Green PCR master mix and an ABIPRISM 7900HT instrument (Applied Biosystems). Expression levels were normalized to endogenous Hprt mRNA levels using the ΔΔCt method ...
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bioRxiv - Plant Biology 2021Quote: ... The PCR products were sub-cloned using the Zero Blunt™ TOPO™ PCR Cloning Kit (Thermo Fisher Scientific) and multiple single clones were Sanger-sequenced (Eurofins Genomics ...
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bioRxiv - Plant Biology 2021Quote: ... The PCR amplification was performed on QuantStudio 6 Flex Real-Time PCR Systems (Applied Biosystems, Foster City, CA, USA) with the following conditions ...
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bioRxiv - Microbiology 2021Quote: ... Each 20-μlL PCR mixture contained 2 μL of 10X Accuprime PCR buffer II (Life Technologies, CA, 1 USA), 0.15 μL of Accuprime high-fidelity polymerase (Life Technologies ...
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bioRxiv - Microbiology 2021Quote: ... qRT-PCR was performed on a Step One Plus Real-Time PCR system (Thermo Fisher, Waltham, MA, United States) with Power SYBR Green Master Mix protocol (Life Technologies ...
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bioRxiv - Microbiology 2021Quote: ... ZIKV RNA levels were determined by quantitative real-time PCR on a 7500 Real-time PCR system (Applied Biosystems). Viral burden was expressed on a log10 scale as viral RNA equivalents per g or per mL after comparison with a standard curve produced using the serial 10-fold dilutions of ZIKV RNA ...
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bioRxiv - Microbiology 2021Quote: ... Genes were amplified by polymerase chain reactions (PCR) using Platinum™ SuperFi II Green PCR mix (Invitrogen, cat# 12369050). The PCR product was digested with restriction enzymes (New England Biolabs ...
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bioRxiv - Microbiology 2021Quote: ... Both first and nested PCRs were performed in 100 μl final volume using Platinum PCR Supermix (ThermoFisher, Kandel, Germany) following a 40-cycle protocol with the following conditions ...
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bioRxiv - Cancer Biology 2020Quote: ... Relative transcript abundance was determined by real-time quantitative PCR using the SYBR Green PCR Master Mix (Applied Biosystems) and gene-specific primers ...
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bioRxiv - Cell Biology 2021Quote: ... Real-time PCR was subsequently performed in ABI 7500 using a Power SYBR green PCR master Mix (Applied Biosystems). The expression level was normalized to the transcript levels of GAPDH ...
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bioRxiv - Cell Biology 2021Quote: ... 1ul of cDNA was used for real-time PCR using TaqMan™ Universal PCR Master Mix (Applied Biosystems, 4364340) on the ABI QuantStudio 7 Flex Real-Time RCR system ...
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bioRxiv - Cell Biology 2020Quote: ... All subsequent qRT-PCR reactions were performed on a QuantStudio 12K Flex Real-Time PCR system (Thermo Fisher Scientific) using the Qiagen QuantiFast SYBR Green PCR kit ...
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bioRxiv - Immunology 2022Quote: ... Data acquisition was performed on the 7500 Fast Real-time PCR system using SYBR Green PCR Master Mix (Thermofisher). Cycle threshold (Ct ...
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bioRxiv - Cell Biology 2022Quote: ... The real-time PCR was performed using Step one Plus real time PCR Assay (Applied Biosystems, Foster City, CA). All reactions were carried out using Power SYBR Green premix (Applied Biosystems).
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bioRxiv - Biochemistry 2022Quote: ... Genotyping of the SNP rs2248374 was performed by quantitative Real-Time Polymerase Chain reaction (qRT-PCR) with functionally tested TaqMan Allelic Discrimination Assay (C_25649529_10; 7300 real-time PCR system, Applied Biosystems).
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bioRxiv - Systems Biology 2022Quote: ... library amplification PCR was initiated by adding 4μl 1x PCR mix consisting of 1x Phusion Buffer (Thermo Scientific F530L), 0.01 U/µL Phusion DNA polymerase (Thermo Scientific) ...
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bioRxiv - Immunology 2021Quote: ... Real-time qRT-PCR was performed using SuperScript™ III Platinum™ One-Step qRT-PCR Kit (Invitrogen, USA) following the manufacturer’s instructions to detect NDV M gene64 and enable the calculation of NDV genome copies.
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bioRxiv - Molecular Biology 2020Quote: Exons and exon-intron boundaries in CARD14 were amplified via PCR using AmpliTaq Gold PCR Master Mix (ThermoFisher Scientific). Primer sequences and PCR conditions are available upon request ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were run in triplicate in 10 μL PCR reactions containing 1X SYBR Green PCR Master Mix (Applied Biosystems), 250 nM forward and reverse primers ...
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bioRxiv - Plant Biology 2021Quote: ... and PpEF1α were cloned into pCR™8/GW/TOPO™ or pCR™-Blunt (Thermo Fisher Scientific, Inc.). To analyze virulence and protein accumulation in N ...
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bioRxiv - Microbiology 2020Quote: ... and cDNA was then subjected to real-time quantitative PCR (qPCR) with ABI 7500 PCR System (Applied Biosystems, USA). Primers were listed in the supplemental material (Supplementary Table 1) ...
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bioRxiv - Neuroscience 2022Quote: ... qRT-PCR amplification of target genes was achieved using Power SYBR Green PCR Master Mix (ThermoFisher, Cat no 4368706) and primer forward/reverse mixes at a final primer concentration of 150 nM in a 10 μL reaction on a 384-well plate ...
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bioRxiv - Pathology 2022Quote: ... Real-time PCR was performed in technical duplicate on a StepOnePlus™ Real-Time PCR System (Applied Biosystems, 4376600) using the Power SYBR® Green PCR Master Mix (Applied Biosystems ...
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Low spontaneous mutation rate in a complex multicellular eukaryote with a haploid-diploid life cyclebioRxiv - Evolutionary Biology 2022Quote: ... The DNAseq libraries were prepared following a PCR-free protocol (Collibri PCR-free PS DNA library prep kit, ThermoFisher) and the genomic DNA of each individual was sequenced by Illumina NovaSeq with 150 bp paired end reads.
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bioRxiv - Evolutionary Biology 2022Quote: ... The resulting PCR product was used as a megaprimer for whole-plasmid PCR using Phusion DNA polymerase (Thermo Fisher) (Sarkar and Sommer 1990) ...
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bioRxiv - Genetics 2022Quote: PCR amplification was performed in a GeneAmp®PCR System 9700 thermal cycler (Thermofisher Scientific, Foster City, CA, USA) on a gold-plated silver block ...
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bioRxiv - Cell Biology 2022Quote: ... PCR analysis of the cDNA was performed on a StepOne Plus Real-Time PCR System (Applied Biosystems, Waltham, Massachusetts). In most of the PCR experiments ...
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bioRxiv - Plant Biology 2022Quote: ... The qRT-PCR measurements were performed using the SYBR Green-PCR Master Mix (Applied Biosystems/Life Technologies, Darmstadt, Germany). Relative expression of each gene was analysed using a comparative cycle threshold (CT ...
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bioRxiv - Biochemistry 2019Quote: ... cDNA quantification was done by real-time PCR on an ViiA 7 real-time PCR system (Thermo Fisher Scientific) using Maxima SYBR Green/ROX qPCR Master Mix (K0222 ...
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bioRxiv - Plant Biology 2019Quote: ... PCR reactions were run on a Gene Amp PCR system 2700 thermocycler (PE Applied Biosystems, Foster City, CA, USA) programmed with the cycling conditions ...
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bioRxiv - Cancer Biology 2019Quote: ... was used for qRT-PCR in a QuantStudio 7 Flex Real-Time PCR System (Thermo Fisher Scientific, Waltham, USA). Data were analyzed by QuantStudio Software V1.3 (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2019Quote: ... Real-time PCR reactions were carried out using the SYBR Green PCR master mix (Applied Biosystems part no. 4309155). The primers used to amplify the p53-target transcripts and MDM2-ALT1 have been previously described (6) ...
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bioRxiv - Microbiology 2019Quote: ... the PCR products of two successful amplifications were pooled and purified with PureLink PCR purification Kit (Invitrogen, CA, USA). A Qubit 2.0 fluorometer (Invitrogen ...
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bioRxiv - Microbiology 2019Quote: ... Gene expression levels were determined by real-time qRT-PCR in a StepOne Real-time PCR System (Applied Biosystems) using the Mesa Blue qPCR Mastermix Plus for Sybr assay kit (Eurogentec) ...
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Loss of p32 triggers energy deficiency and impairs goblet cell differentiation in ulcerative colitisbioRxiv - Molecular Biology 2020Quote: ... Target amplification was performed by quantitative reverse transcriptase PCR (qRT-PCR) on the StepOne real-time system (ThermoFisher Scientific) applying Perfecta SYBR Green Supermix (ThermoFisher Scientific ...
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bioRxiv - Plant Biology 2021Quote: ... Quantitative PCR was performed in 96-well optical plates in a HT 7900 Real Time PCR system (Applied Biosystems) using standard thermocycler conditions ...
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bioRxiv - Immunology 2020Quote: ... PCR was performed to determine RNA copies/mL using the Superscript III Platinum One-Step qRT-PCR system (Invitrogen). Standard curves were obtained by using a quantitative PCR (qPCR ...
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bioRxiv - Microbiology 2020Quote: ... All PCRs were run in triplicate on a StepOne Plus real-time PCR system (Applied Biosystems Inc., Carlsbad, CA).
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bioRxiv - Evolutionary Biology 2020Quote: ... The 25 μl PCR mixture consisted of 12.5 μl SYBR Green PCR Master Mix (Life Technologies, Warrington, United Kingdom), 1 μM each of the forward and reverse primers ...
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bioRxiv - Molecular Biology 2019Quote: ... The qRT-PCR amplification reactions were performed by using the Applied Biosystems StepOne PCR System (Thermo Fisher Scientific, USA) and SYBR Premix Ex Taq™ (TakaRa ...
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bioRxiv - Cancer Biology 2019Quote: ... Quantitative real-time PCR was performed following the manufacturer’s protocol by using Power SYBR Green PCR Mix (Applied Biosystems) and StepOnePlus Real-time PCR system (Applied Biosystems) ...
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bioRxiv - Microbiology 2021Quote: ... and qRT-PCR was performed using Fast SYBR Green Master Mix and 7500 Real-Time PCR System (Applied Biosystems). Gene expression was determined using the 2-ΔΔCt method and normalised to GAPDH expression ...
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bioRxiv - Pathology 2021Quote: ... Real-time PCR was performed in technical triplicate on a StepOnePlus™ Real-Time PCR System (Applied Biosystems, 4376600) or on a BioRad CFX96 Real-Time System using the Power SYBR® Green PCR Master Mix (Applied Biosystems ...
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bioRxiv - Synthetic Biology 2021Quote: ... Custom parts for the toolkit were generated by PCR amplification (Phusion Flash High-Fidelity PCR Master Mix; Thermo Scientific) and assembled into toolkit vectors via golden gate assembly [48] ...
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bioRxiv - Plant Biology 2021Quote: ... PCR reactions performed with standards using QuantStudio 3 Real-time PCR (Applied Biosystems-Thermo Fisher Scientific Waltham, MA, USA) yielded cycle threshold values (Ct) ...
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bioRxiv - Genomics 2020Quote: ... Quantitative real-time PCR (qRT-PCR) was performed using TaqMan probes (Csn1s2a, Mm00839343_m1; Csn1s2b, Mm00839674_m1; mouse Gapdh, Mm99999915_g1, Thermo Fisher scientific) on the CFX384 Real-Time PCR Detection System (Bio-Rad ...