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Citations for Thermo Fisher :
9001 - 9050 of 10000+ citations for rno mir 134 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... slices were incubated for 2 hours at RT with streptavidin Alexa488 conjugate (S11223, Molecular Probes, dilution 1:500). Washings ...
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bioRxiv - Microbiology 2021Quote: ... and human RNase P (RP assay) together with TaqPath™ 1-Step RT-qPCR Master Mix CG (ThermoFisher). A plasmid containing the complete nucleocapsid gene from 2019-nCoV (IDT ...
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bioRxiv - Microbiology 2021Quote: ... Samples were cooled to RT and released RNA was purified by standard phenol/chloroform extraction (Thermo Fisher Scientific) following manufacturer’s instructions ...
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bioRxiv - Genetics 2022Quote: 5 µL of RT-qPCR reaction was digested with 1 µL FastDigest PstI restriction enzyme (#FD0614, ThermoFisher Scientific) for 1 h at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... cells were washed and incubated for 1 h at RT with the secondary antibody (Alexafluor 488/568, Invitrogen). Images were captured either with a confocal laser scanning microscope (CLSM ...
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bioRxiv - Molecular Biology 2020Quote: ... RNA was reverse-transcribed by addition of 3 μl of RT mix (2 μl 5x SSIV buffer [Invitrogen] ...
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bioRxiv - Microbiology 2021Quote: ... lipid films were brought to RT and hydrated in extrusion buffer (250 mM Raffinose pentahydrate (Fisher Scientific; ICN10279725), 50 mM TRIS 150 mM NaCl ...
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bioRxiv - Plant Biology 2021Quote: ... cDNA was synthesized using the Maxima first strand cDNA synthesis kit for RT-qPCR with DNase (Thermo Scientific) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... 0.5 to 2 μg of RNA was reverse transcribed with the high-capacity cDNA RT kit (Applied Biosystems), using random hexamers ...
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bioRxiv - Bioengineering 2022Quote: ... RT-qPCR was performed using the TaqMan Fast Advanced Master Mix (Cat# 4444556, Applied Biosystems, Foster City, CA). U6 snRNA (Cat# 4444556 ...
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bioRxiv - Cell Biology 2022Quote: ... Retrotranscription (RT) (4 μg of total RNA) was performed with the High-Capacity cDNA Archive Kit (Applied Biosystems). For quantitative real-time PCR (qPCR) ...
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In vivo imaging of the kinetics of microglial self-renewal and maturation in the adult visual cortexbioRxiv - Neuroscience 2020Quote: ... clone G-A-5) followed by a secondary antibody solution (4 h, RT, AlexaFluor 594, 1:500, Invitrogen), mounted and coverslipped ...
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Adaptive translational pausing is a hallmark of the cellular response to severe environmental stressbioRxiv - Molecular Biology 2020Quote: ... cooled on ice and incubated in 20 µL RT mix (300 U Superscript III(Thermo Fisher Scientific, 18080044), 1X RT buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... the plugs were incubated at RT for 30 min with 1 μL of YOYO-1 (Molecular Probes, Y3601) in 150 μL of TE50 buffer (10 mMTris-HCl pH 7.0 ...
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bioRxiv - Molecular Biology 2020Quote: ... in PBS for 15 mins at RT and quenched in 100 mM glycine (G/0800/60, Fisher Scientific). For total protein expression neurons were fixed in 4% (vol/vol ...
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bioRxiv - Plant Biology 2021Quote: ... cDNA was synthesized using as a template 2 μg of RNA following the SuperScript II RT protocol (Invitrogen). To amplify the cDNA ...
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bioRxiv - Microbiology 2021Quote: ... cDNA was generated using the High-Capacity cDNA Reverse Transcription Kit or H Minus RT kit (Thermo Fisher). Two regions of the viral genome of SARS-COV-2 were amplified ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were then again washed 3 x 15 min in WB at RT with DAPI (Thermo Scientific #62248) diluted to 1 ug/ml in the final WT wash ...
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bioRxiv - Neuroscience 2021Quote: ... tissues were incubated for 1 hr at RT with Alexa-Fluor 488 goat anti-mouse (Invitrogen cat# A11012), Alexa-Fluor 488 donkey anti-rabbit (Invitrogen cat# A-21206) ...
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bioRxiv - Neuroscience 2021Quote: ... the samples were evaluated at RT with a Talos L120C transmission electron microscope (Thermo Fisher Scientific, MA, USA).
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bioRxiv - Microbiology 2020Quote: ... cDNA was synthesized from the total RNA using a high capacity cDNA reverse transcription (RT) kit (ThermoFisher Scientific) according to the manufacturer’s specifications ...
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bioRxiv - Microbiology 2021Quote: ... RT–qPCR reactions were performed using the PowerUp SYBG Green Master Mix Kit (Applied Biosystems, Waltham, MA, USA), in which samples were processed in duplicate using the following cycling protocol ...
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bioRxiv - Developmental Biology 2022Quote: 100 ng RNA isolated from E9.5 and E10.5 tailbud tissue was used as template for cDNA synthesis performed using RT-Vilo (ThermoFisher). Quantitative PCR was performed using the 96*96 BioMark Fluidigm format ...
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bioRxiv - Cell Biology 2020Quote: Gene expression was analysed via reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) using TaqMan probes (Life Technologies) and SSO Universal Probes Master Mix (Bio-Rad) ...
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bioRxiv - Immunology 2022Quote: ... mRNA-trapped beads were suspended in 10 µL of RT mix [1× First Strand buffer (Thermo Fisher Scientific), 1 mM dNTP (Roche) ...
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bioRxiv - Immunology 2022Quote: ... Plates were developed for 5-15 min at RT using 100µl of TMB (Invitrogen Ref. 50-112-9758) and the reaction stopped with H2SO4 ...
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bioRxiv - Microbiology 2022Quote: ... 3 µL RT reaction mix consisting of 3 µL of 50 µM random hexamers (Thermo Fisher, cat#48190011), 0.8 µL of 25 mM deoxyribonucleotide triphosphates (dNTPs ...
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bioRxiv - Immunology 2019Quote: ... the cells were incubated for 30 min at RT with the following anti-mouse Abs (Thermo Fisher Scientific): APC-labeled anti-IFNγ (XMG1.2 ...
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bioRxiv - Synthetic Biology 2019Quote: ... incubated for 5 minutes at RT and transferred to the DynaMag-96 Side Magnet (#12331D, Thermo Fisher Scientific). Following incubation of the sample on the magnet for 2 minutes (or until sample is clear) ...
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bioRxiv - Cancer Biology 2019Quote: ... The RT-qPCR assays were completed using the ABI ViiA™ 7 sequence detection system (Applied Biosystems®) with the following parameters ...
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bioRxiv - Immunology 2020Quote: ... activated for 30 min on a rotor at RT by addition of Sulfo-N-Hydroxysulfosuccinimide (Thermo Fisher Scientific) and 1-Ethyl-3-(3-dimethylaminopropyl ...
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bioRxiv - Molecular Biology 2020Quote: RT-RCA was performed in 20 µL with Maxima H Minus Reverse Transcriptase (Thermo Fisher, cat n°EP0752) under the following conditions ...
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bioRxiv - Plant Biology 2021Quote: ... cDNA was synthesized using as a template 2 μg of RNA following the SuperScript II RT protocol (Invitrogen). To amplify the cDNA ...
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bioRxiv - Molecular Biology 2021Quote: ... All RNA samples to be used in reverse transcriptase (RT) reactions were treated with turbo DNase (Thermo Fisher) followed by re-isolation with TRIzol ...
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bioRxiv - Microbiology 2020Quote: ... 0.5 to 2 μg of RNA was reverse transcribed with the high-capacity cDNA RT kit (Applied Biosystems), using random hexamers for amplification of cellular and viral genes ...
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bioRxiv - Microbiology 2021Quote: ... Reverse transcription (RT) of total RNA was performed using the High-Capacity cDNA Reverse Transcription kit (Thermo Fisher) with random hexamers according to the provided protocol ...
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bioRxiv - Plant Biology 2020Quote: ... Temperature was then reduced to 50°C and SSIV RT enzyme mix (Thermo Fisher Scientific, Waltham, MA, USA) was added according to the manufacturer’s protocol for First-Strand cDNA Synthesis Reaction ...
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bioRxiv - Molecular Biology 2019Quote: ... for 5 minutes and triturated 10x in 1ml RT DNase buffer (0.5g/L DNase, 0.35% Glucose, in BME –GIBCO-) to obtain single cell suspension ...
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bioRxiv - Neuroscience 2019Quote: ... reverse transcription was performed using the Maxima First Strand cDNA Synthesis Kit for RT-qPCR (Thermo Fisher Scientific). The quality of the cDNA was tested using GPDH primers (see below for sequence ...
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bioRxiv - Immunology 2021Quote: ... Quantification of viral burden was done using TaqPath 1-step RT-qPCR Master Mix (Thermo Fisher Scientific, USA) and primers/probes sets targeting SIVmac239 (Eurofins Scientific ...
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bioRxiv - Physiology 2021Quote: ... The columns were first equilibrated at room temperature (RT) with 10 mL of 0.22 µm filtered PBS (Gibco), then 500 µL of CM or EV resuspension from UC or UF was loaded on the column and eluted in filtered PBS ...
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bioRxiv - Microbiology 2021Quote: ... RT was performed by using the SuperScript® III First-Strand Synthesis System (ThermoFisher Scientific, Catalogue number 18080051) with gene specific primers (SEM296 5’-ctttaattaactgcttcaacc-3’ ...
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bioRxiv - Immunology 2020Quote: ... for 30 min at room temperature (RT) and stained with 0.5 µM of the YOYO-1 dye (Invitrogen) for 30 min at RT and directly analyzed on a BD FACSCanto II (Becton Dickinson ...
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bioRxiv - Immunology 2021Quote: ... incubated with proper secondary antibody for 1 h at RT and mounted using proGold anti-bleaching solution (Thermofisher).
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bioRxiv - Cell Biology 2021Quote: ... Then RNA was reverse transcribed using Maxima First Strand cDNA Synthesis Kit for RT-qPCR (Thermo Fisher scientific). The cDNA was PCR amplified using specific primers for TOPO cloning ...
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bioRxiv - Cancer Biology 2022Quote: ... membranes were blocked for 1 hr at RT in 5% albumine bovine fraction V (Thermo Scientific, pH 7.0) washed in TBS + 0.05% Tween with pH 8.0 (TBS-T ...
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bioRxiv - Genomics 2022Quote: ... Quantitative real-time polymerase chain reaction (RT-qPCR) was performed using Fast SYBR Green Master Mix (Applied Biosystems) and custom intron-spanning primers (Integrated DNA Technologies ...
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bioRxiv - Neuroscience 2022Quote: Total RNA (500ng to 2μg) was then reverse transcribed using a high-capacity cDNA RT kit (Applied Biosystems) according to the manufacturers’ protocol.
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bioRxiv - Microbiology 2023Quote: ... Reverse transcription (RT) of total RNA was performed using the High-Capacity cDNA Reverse Transcription kit (Thermo Fisher) with random hexamers or gene-specific primers (Table 1 ...
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bioRxiv - Immunology 2022Quote: ... Cells were washed with PBS, BSA (0.5%), incubated (5 min, RT) with a viability dye (Aqua-dye, Invitrogen) and surface stained with titrated amounts of antibodies for 30 min at room temperature ...