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Citations for Thermo Fisher :
8951 - 9000 of 10000+ citations for rno mir 134 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... Genes encoding chloroplast proteins rbcL and psbA were amplified by PCR using a ProFlex PCR System (Thermo Fisher Scientific). The primers were based on a previous study of Faria et al ...
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bioRxiv - Cell Biology 2023Quote: ... qRT-PCR was carried out using THUNDERBIRD SYBR qPCR Mix (TOYOBO) on 7300 Real Time PCR System (Applied Biosystems). ACT8 (At1g49240 ...
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bioRxiv - Synthetic Biology 2024Quote: The reaction was performed using a real-time PCR system (StepOnePlus Real-Time PCR System, Life Technologies, CA, USA) by incubating at 37°C ...
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bioRxiv - Molecular Biology 2024Quote: ... and analyzed by quantitative real-time PCR (qRT-PCR) using TaqMan® Fast Advanced Master Mix ( Thermo Fisher Scientific) and MDM2 TaqMan probe (Hs01066930_m1 ...
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bioRxiv - Physiology 2023Quote: ... MA. qRT-PCR analysis was done using Power SYBR Green PCR Master Mix (Cat. No. 4367659) from Applied Biosystems, Waltham ...
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bioRxiv - Molecular Biology 2023Quote: ... The ChIP products were analyzed by real-time PCR using SYBR Green Realtime PCR Master Mix (Applied Biosystems, USA) under thermal cycling as 1 min at 95 ℃ followed by 45 cycles of 15 s at 95℃ ...
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bioRxiv - Plant Biology 2023Quote: ... A quantitative real-time PCR assay was performed on a 7500 real-time PCR system (Applied Biosystems, CA, USA) using iTaq Universal SYBR Green Supermix (BioRad) ...
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bioRxiv - Cancer Biology 2023Quote: ... Quantitative real-time PCR was carried out using an ABI Step One Plus Real-Time PCR System (Applied Biosystems). Reactions for each sample were performed in triplicate ...
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bioRxiv - Cell Biology 2023Quote: ... The PCR product was ligated into pCR™4 vector using TOPO™ TA Cloning™ Kit (Invitrogen, 450030). After transforming into DH5α competent cells ...
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bioRxiv - Cell Biology 2023Quote: ... The PCR and data acquisition were performed with a StepOnePlus™ Real-Time PCR System (Applied Biosystems, cat.no. 4376600). For each biological replicate of a given experiment ...
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bioRxiv - Microbiology 2023Quote: ... cDNA levels were quantified by quantitative real-time PCR (qRT-PCR) on an Applied Biosystems light cycler (Applied Biosystems) using a SYBR Green PCR Master Mix (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2023Quote: ... the coding sequence of each protein was PCR-amplified and subcloned into pCR™8/GW/TOPO® (Invitrogen). To generate binary plasmids ...
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bioRxiv - Microbiology 2023Quote: ... PCR products were then analysed by agarose gel electrophoresis and purified using the GeneJET PCR purification kit (Thermo Scientific). The purified PCR products were subsequently cloned using the TOPO TA Cloning® kit (Invitrogen ...
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bioRxiv - Genetics 2023Quote: ... Real-time PCR was performed for each target and sample in duplicate on a StepOnePlus PCR System (Applied Biosystems) with primers from TaqMan™ Advanced miRNA Assay (#A25576 ...
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bioRxiv - Physiology 2024Quote: ... Real-time PCR was performed with a Step One Plus Detection System (Real-Time PCR System-Thermo Fisher Scientific). The results represent the average from three technical replicates for each analyzed animal ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and sim1a was measured by quantitative PCR using a Real time PCR system (Applied Biosystems by Thermo Fisher Scientific) with PowerUp SYBER Green Master Mix (Applied Biosystems by Thermo Fisher Scientific ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and sim1a was measured by quantitative PCR using a Real time PCR system (Applied Biosystems by Thermo Fisher Scientific) with PowerUp SYBER Green Master Mix (Applied Biosystems by Thermo Fisher Scientific ...
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bioRxiv - Genetics 2024Quote: ... qRT-PCR was performed using Power Up SYBR green Master mix (Invitrogen; 7900HT Fast Real-Time PCR Applied Biosystems). Samples were normalized to 18S RNA and/or luciferase and relative mRNA levels were calculated using the 2-ΔΔCT method ...
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bioRxiv - Cancer Biology 2024Quote: ... Quantitative real-time PCR (qPCR) was performed using the Power SYBR Green PCR Master Mix (Applied Biosystems, Cat#4368708) and detected on the QuantStudio 5 Real-Time PCR system (Applied Biosystems) ...
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bioRxiv - Cell Biology 2024Quote: ... Quantitative PCR (qPCR) was conducted on a QuantStudio™ 6 Flex Real-Time PCR System (Applied Biosystems, CA, USA). Relative mRNA levels were determined using the Comparative Ct (△△CT ...
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bioRxiv - Microbiology 2024Quote: ... Genotype of mice was confirmed with PCR where DNA was extracted from mouse tissue using Direct PCR (Thermo Fisher) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: A duplex real-time PCR (ABI Prism Step One real-time PCR apparatus, Thermo Fisher Scientific, Waltham, MA, USA) was performed in triplicates of 1:10 diluted cDNA samples with predesigned FAM-labeled TaqMan® assays (Applied biosystems by Thermo Fisher Scientific ...
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bioRxiv - Genetics 2024Quote: ... Real-time PCR was performed for each target and sample in duplicate on a StepOnePlus PCR System (Applied Biosystems) with primers from TaqMan™ Advanced miRNA Assay ...
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bioRxiv - Microbiology 2024Quote: ... The cDNA was amplified using SYBR green-based real-time PCR on a Quantstudio 12K PCR system (Applied Biosystems). Relative mRNA levels (2−ΔΔCq ...
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bioRxiv - Plant Biology 2020Quote: ... cDNA was synthesized using the Maxima first strand cDNA synthesis kit for RT-qPCR with DNase (Thermo Scientific) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... for 1 h at RT and incubated with a 1:50 dilution of phalloidin-AF647 (Thermo Fisher Scientific) for 96 h at 4 °C ...
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bioRxiv - Cell Biology 2020Quote: ... Coverslips were next incubated with secondary antibodies for 1 hr in RT and mounted with Fluoromount-G (Invitrogen) on slideglasses ...
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bioRxiv - Developmental Biology 2021Quote: ... mRNAs captured by DNBs on the chip were reverse transcribed with SuperScript II reverse transcription (RT) mix (Invitrogen, 18064-014 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... to remove genomic DNA contaminants and then converted to cDNA using Applied Biosystems High Fidelity RT kit (ThermoFisher). Quantitative real time PCR (qPCR ...
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bioRxiv - Physiology 2022Quote: ... Sections were blocked at RT with 10% normal goat serum (NGS) (Thermo Fisher Scientific, 50062Z, Waltham, MA, USA) (60 minutes for myosin heavy chain (MyHC) ...
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bioRxiv - Microbiology 2022Quote: RT-qPCR was performed with the help of DyNAmo Flash SYBR green kit (Thermo Fisher Scientific, Waltham, MA) to validate the relative miRNA expression ...
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bioRxiv - Neuroscience 2021Quote: ... RNA was extracted using Qiagen RNeasy Kit and cDNA was prepared using the SuperScript IV RT Kit (ThermoFisher). The cDNA was used for amplifying fmn2b transcripts in the mnx1 positive motor neurons ...
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bioRxiv - Molecular Biology 2021Quote: ... Equal fraction of total RNA per oocyte/zygote was reverse transcribed by SuperScript III RT (Thermo Fisher Scientific) with random hexamers as recommended by the manufacturer ...
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bioRxiv - Neuroscience 2020Quote: ... followed by 30 min RT incubation with anti-Mouse IgM 594 (Invitrogen, CA, US; Cat. No. A-21044) 1 ...
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bioRxiv - Molecular Biology 2021Quote: ... and incubation in the second strand synthesis buffer (Maxima RT: Life Technologies, Inc., EP0753; 10mM dNTP Life Technologies, Inc. ...
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bioRxiv - Genetics 2019Quote: ... RT products were diluted with H2O and used for qPCR analysis on an ABI 7300 system (Life Technologies) (technical triplicates) ...
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bioRxiv - Molecular Biology 2019Quote: ... and RT-qPCR was performed using Platinum™ SYBR™ Green qPCR SuperMix-UDG (Invitrogen, now ThermoFisher Scientific) together with site specific primers ...
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bioRxiv - Molecular Biology 2019Quote: ... and RT-qPCR was performed using Platinum™ SYBR™ Green qPCR SuperMix-UDG (Invitrogen, now ThermoFisher Scientific) together with site specific primers ...
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bioRxiv - Immunology 2019Quote: ... cDNA was generated from 2.5 nanograms total RNA using a high capacity cDNA RT kit (Applied Biosystems, 4368814) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2019Quote: ... cells were incubated at RT for 1 h in phalloidin conjugated with Alexa Fluor 546 (A22283; Life Technologies) diluted in buffer 1 ...
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bioRxiv - Cancer Biology 2019Quote: ... Total RNA reverse transcription (RT) was performed using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, USA). After reverse transcription ...
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bioRxiv - Genetics 2021Quote: ... Reverse transcription (RT) was carried out using the High-Capacity cDNA Reverse Transcription Kit (Life Technologies, NY, USA). One microgram of mRNA was reverse transcribed to cDNA according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: ... The resulting DNA-free total RNA was subjected to in vitro Reverse Transcription (RT) with Superscript III (Invitrogen). 1µg of total RNA was used for each reaction and assays were conducted in biological triplicates ...
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bioRxiv - Evolutionary Biology 2021Quote: ... RT-qPCR was carried out with a Power SYBR Green RNA-to-Ct 1-Step Kit (Applied Biosystems). UBQ10 (AT4G05320 ...
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bioRxiv - Immunology 2021Quote: ... RNA was extracted and first strand cDNA was synthesized using the superscript III RT kit (Invitrogen™, USA). The primers used for qPCR were retrieved from the primer bank found at http://pga.mgh.harvard.edu/primerbank/ ...
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bioRxiv - Genomics 2020Quote: ... Buffer was removed and beads were subsequently washed in 200 μl of 5x Maxima RT buffer (Life Technologies).
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bioRxiv - Neuroscience 2021Quote: ... Coverslips were blocked for one hour at RT in buffer containing 5% normal goat serum (NGS) (ThermoFisher: PCN5000) and 0.3% Triton-X in 1x PBS and incubated in primary antibodies in antibody dilution buffer (1% BSA and 0.3% Triton-X in 1x PBS ...
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bioRxiv - Neuroscience 2020Quote: ... cDNA was synthesized from 2 µg of RNA using High Capacity cDNA RT kit (Applied Biosystems, cat#4368814). qPCR was performed in a CFX96 Real-time system (Bio-Rad ...
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bioRxiv - Cell Biology 2021Quote: ... and incubated (1 hour at RT) with horseradish peroxidase-labeled goat anti-rabbit secondary antibodies (Thermo Fisher Scientific) diluted 1:2,500 in 50% blocking buffer ...
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bioRxiv - Neuroscience 2020Quote: ... at 42 °C with rotation in RT mix with 2000 U Maxima H Minus Reverse Transcriptase (ThermoFisher, MA) in 200 μl total volume ...