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Citations for Thermo Fisher :
8901 - 8950 of 10000+ citations for rno mir 134 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Rac1, Rac3 GTPases and TPC2 are required for axonal outgrowth and migration of cortical interneuronsbioRxiv - Neuroscience 2022Quote: ... Real time PCR analysis was performed using a Step One Plus real-time PCR system (Applied Biosystems, Life Technologies, Thermo Fisher Scientific Inc. ...
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bioRxiv - Cancer Biology 2022Quote: ... no UNG was used and qRT-PCR was programmed using an QuantStudio 3 real-time PCR system (ThermoFisher Scientific). Data were normalized by expression level of each sample as previously described (Schmittgen and Livak 2008).
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bioRxiv - Cell Biology 2022Quote: ... The PCR was carried out on 384-well plates on a Quant Studio Real Time PCR system (Applied Biosystems) using a three-stage program ...
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bioRxiv - Cancer Biology 2022Quote: ... qRT-PCR reaction was carried out in 384-well format using 7900HT Fast Real-Time PCR System (Applied Biosystems) and Power SYBR Green PCR Master Mix (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... qRT-PCR was performed on the A7500 Fast Real-Time PCR System (Applied Biosystems Co. Ltd, New York, USA) with the gene-specific primers (Table S1) ...
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bioRxiv - Plant Biology 2022Quote: ... The qRT-PCR measurements were performed using the SYBR Green-PCR Master Mix (Applied Biosystems/Life Technologies, Darmstadt, Germany). Relative expression of each gene was analysed using a comparative cycle threshold (CT ...
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bioRxiv - Molecular Biology 2023Quote: ... and a 7900HT Fast Real Time PCR System or a Quant Studio 5 Real Time PCR System (Applied Biosystems) with the primers listed in Supplemental Table S1 ...
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bioRxiv - Plant Biology 2023Quote: ... followed by cloning of PCR products into a cloning vector (Zero Blunt™ TOPO™ PCR Cloning Kit, Invitrogen) and Sanger sequencing ...
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bioRxiv - Immunology 2022Quote: ... qRT-PCR was performed by a QuantStudio 6 Flex real-time PCR detection system (Applied Biosystems, Foster City, CA) with SYBR Premix Ex TaqTM II (Tli RNaseH Plus ...
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bioRxiv - Neuroscience 2022Quote: ... PCR was carried out on duplicate samples by using a StepOne real-time PCR system (Applied Biosystems, Tokyo, Japan) under the following conditions ...
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bioRxiv - Molecular Biology 2022Quote: ... the reaction volume was 25 μL and PCR was conducted using the 7500 Real-Time PCR System (Applied Biosystems). Three independent biological replicates per sample and three technical replicates per biological replicate were analyzed ...
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bioRxiv - Molecular Biology 2022Quote: ... qRT-PCR assays were performed with SuperScript III Platinum SYBR Green One-Step qTR-PCR Kit (Invitrogen Cat#11736051) according to vendor’s standard procedure ...
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bioRxiv - Plant Biology 2022Quote: ... The PCR product ligated to pJet1.2 intermediate plasmid following the instruction of CloneJET PCR Cloning Kit (K1232, Thermo Scientific). The pOsZAS2 sequence was amplified from the pJet1.2 plasmid with the specific primers (Supplemental Table S1 ...
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bioRxiv - Biochemistry 2024Quote: ... the PCR reaction was mixed by combining 25 μL of Platinum II Hot-Start PCR master mix (ThermoFisher #14000012), two μL of the diluted middle primer solution ...
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bioRxiv - Neuroscience 2024Quote: ... and either Power SYBR Green PCR Master Mix or Taqman Universal PCR Master Mix (both Thermo Fisher Scientific, USA). Relative quantification of mRNA and miRNA was calculated by the 2-ΔΔCt method ...
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bioRxiv - Developmental Biology 2024Quote: ... The Taqman real-time PCR was carried out using Applied Biosystems QuantStudio 7 Flex Real-Time PCR System (Thermofisher), and all the procedures were followed as per the instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Quantitative real-time PCR (qPCR) amplification of cDNA was performed with the SYBR Green PCR Master Mix (ThermoFisher Scientific) on a QuantStudio 6 Flex Real-Time PCR system (ThermoFisher Scientific) ...
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bioRxiv - Cancer Biology 2024Quote: ... Quantitative real-time PCR (qRT-PCR) reaction was carried out with Step-One Plus system (Thermo Fisher Scientfic, USA).
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bioRxiv - Cancer Biology 2024Quote: ... The resulting cDNA was then used for quantitative PCR (qPCR) using SYBR™ Green PCR Master Mix (Applied Biosystems), depending on the specific gene targets ...
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bioRxiv - Microbiology 2024Quote: ... The absence of genomic DNA was confirmed by conventional PCR using DreamTaq Green PCR Master Mix (Thermo Fisher Scientific) and primers 16s-533-F and 16s-1100-R targeting the 16S rRNA gene ...
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bioRxiv - Molecular Biology 2024Quote: ... were used to perform real-time PCR on a 7900HT Fast Real-Time PCR System (Applied Biosystems, Waltham, MA). Glyceraldehyde-3-phosphate dehydrogenase (GAPDH ...
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bioRxiv - Systems Biology 2024Quote: ... PCR was performed using the Phusion Hot Start II High Fidelity PCR Master Mix (Thermo Scientific, Waltham, MA, USA) with 100 pg template ...
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bioRxiv - Microbiology 2024Quote: ... Quantitative PCR was performed on a ABI7500 Real-Time PCR System (Applied Biosystems, Life Technologies, Villebon-sur-Yvette, France). Data were normalized using 36B4 as RPLP0 housekeeping gene ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The amplification steps were conducted in 96 well-PCR plates on ABI 7500 real-time PCR System (Applied Biosystems). The primer efficiency calculation ...
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bioRxiv - Microbiology 2024Quote: ... DNA amplification was performed by PCR using DreamTaq MasterMix (2X) or High-Fidelity PCR Enzyme Mix (Thermo Fisher Scientific). The primers used are listed in Supplementary Table S2 ...
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bioRxiv - Microbiology 2024Quote: ... Quantitative PCR was performed on a ABI7500 Real-Time PCR System (Applied Biosystems, Life Technologies, Villebon-sur-Yvette, France). Data were normalized using 36B4 as RPLP0 housekeeping gene ...
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bioRxiv - Microbiology 2024Quote: ... The PCR products were then cloned into the pCR™4-TOPO® TA cloning vector (Thermo Fisher Scientific) and the clones were confirmed by restriction digestion with BamHI ...
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bioRxiv - Microbiology 2024Quote: ... Intracellular viral DNA was measured by real-time quantitative (q) PCR using SYBR Green PCR Master Mix (Thermo Scientific) and GAPDH was the internal control ...
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Linear ubiquitination at damaged lysosomes induces local NF-κB activation and controls cell survivalbioRxiv - Cell Biology 2023Quote: ... SYBR green-based qRT-PCR was performed using the SYBR™ Green PCR Master Mix (Thermo Fisher Scientific, #4312704) and the 7900GR Fast Real-time PCR system (Applied Biosystems ...
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bioRxiv - Immunology 2023Quote: ... Real time quantitative PCR analysis was performed using a QuantStudio 3 real time PCR system (Applied Biosystems, Waltham, MA) using a Radiant qPCR mastermix (Alkali Scientific ...
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bioRxiv - Plant Biology 2023Quote: ... The final PCR products (round 4) were subcloned into the pJET vector (CloneJET PCR Cloning Kit, Thermo Fisher Scientific) and sequenced ...
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bioRxiv - Genomics 2023Quote: ... Quantitative real-time PCR (qRT-PCR) was performed using TaqMan probes (Csn2, Mm04207885_m1; Csn1s2a, Mm00839343_m1; mouse Gapdh, Mm99999915_g1, Thermo Fisher scientific) on the CFX384 Real-Time PCR Detection System (Bio-Rad ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Real-time PCR was performed for each sample using the StepOnePlus™ Real-Time PCR System (Thermo Fisher Scientific). β-actin (Actb ...
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bioRxiv - Molecular Biology 2023Quote: ... and PCR amplification was performed with the 7500 Fast Real-Time PCR System (Applied Biosystems, Foster City, CA, USA) using the following program ...
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bioRxiv - Molecular Biology 2023Quote: ... and amplified on a 7500 Fast Real-Time PCR system using Fast SYBR Green PCR Master Mix (Applied Biosystems). The sequences for primers are included in Appendix Table S2 ...
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bioRxiv - Plant Biology 2023Quote: ... PCR products were cloned to pJET1.2/blunt vectors using the CloneJET PCR Cloning kit (Thermo Scientific, Cat. Nos. K1231). Cloned plasmids were transformed into E ...
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bioRxiv - Cell Biology 2023Quote: ... The real-time PCR reactions were performed using the QuantStudio™ 3 Real-Time PCR System (Thermo Fisher Scientific).
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bioRxiv - Molecular Biology 2023Quote: ... Thermo Scientific’s SYBR green Real-time PCR master mix was used to perform qRT-PCR (Quantstudio 6, ThermoFisher Scientific) in triplicates to determine the fusion gene expression levels using PRCC-TFE3 fusion gene primers (FP:5’-CCAAGCCAAAGAAGAGGAAA-3’ and RP:5’- CGAGTGTGGTGGACAGGTACT-3’).
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bioRxiv - Systems Biology 2023Quote: ... Relative transcript quantifications were done by real-time PCR on an ViiA 7 Real-Time PCR System (Thermo Fisher) using Maxima SYBR Green/ROX qPCR Master Mix (K0222 ...
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bioRxiv - Microbiology 2023Quote: ... All PCR reactions were carried out using Phusion Hot Start II High-Fidelity PCR Master Mix (Thermo Fisher, USA). The universal bacterial 16s rRNA gene amplicon PCR primers were used ...
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bioRxiv - Plant Biology 2023Quote: ... Selected PCR amplicons were purified using ExoSAP-IT™ PCR Product Cleanup Reagent (Thermo Fisher Scientific, Waltham, MA, USA) and sequenced with Sanger sequencing (Genewiz ...
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bioRxiv - Neuroscience 2023Quote: ... Gene expression was determined by quantitative real-time PCR using QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems). Relative gene expression was determined using ΔΔCT method ...
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bioRxiv - Cell Biology 2023Quote: ... All qRT-PCRs were carried out on a Step One Plus Real-Time PCR System (Applied Biosystems, Life Technologies). Primers used are as follows ...
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bioRxiv - Cell Biology 2023Quote: ... All qRT-PCRs were carried out on a Step One Plus Real-Time PCR System (Applied Biosystems, Life Technologies). Primers used are as follows ...
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Human CCR6+ Th cells show both an extended stable gradient of Th17 activity and imprinted plasticitybioRxiv - Immunology 2023Quote: ... Real-time PCR was performed on samples in duplicate on an ABI 7900HT Real-Time PCR System (Applied Biosystems) or Eco Real-Time PCR System (Illumina ...
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bioRxiv - Developmental Biology 2023Quote: ... The PCR product from the Gibson assembly was purified and cloned into pJet1.2 plasmid (CloneJET PCR Cloning Kit, ThermoFisher). Then the loxP-dsRed-loxP fragment was cloned from pMSCV-loxP-dsRed-loxP-3xHA-Puro-WPRE (Addgene plasmid #32703) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... PCRs were performed in 50 mL of volume containing 25 mL of 2X DreamTaq Green PCR Master Mix (ThermoFisher), 2.5 mL of 10 mM forward and reverse primers ...
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bioRxiv - Developmental Biology 2023Quote: ... Real-time PCR was performed using an ABI 7500 thermocycler with Power SYBR Green PCR Kit (Thermo Fisher, 4368702). The relative expression of interested genes is normalized to the expression of 18S rRNA or β-actin ...
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bioRxiv - Plant Biology 2023Quote: ... qRT-PCR experiments were conducted on an ABI 7500 real-time PCR instrument (Applied Biosystems, Foster City, CA, USA) using UltraSYBR Green Mixture reagent (ComWin Biotech ...
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bioRxiv - Genetics 2023Quote: ... were amplified by PCR as previously described 64 and purified using a GeneJET PCR Purification Kit (Thermo Scientific, K0702). 600 ng of purified DNA template was in vitro transcribed for 4 h at 37°C using MEGAshortscript T7 Transcription Kit (Invitrogen ...