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Citations for Thermo Fisher :
8851 - 8900 of 9427 citations for Vertical Gel Casting Cassettes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2019Quote: ... DNA quality was assessed by gel electrophoresis (1% agarose in 1 × TAE containing ethidium bromide) and quantity was determined by Qubit Fluorometry (Invitrogen, California, USA) following manufacturer’s instructions.
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bioRxiv - Synthetic Biology 2019Quote: ... 20 μL of the supernatant containing the extracted polypeptides was loaded on a NuPAGE Bis Tris 4-12% gels (Thermo Fisher Scientific) for gel electrophoresis and Western blot analysis.
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bioRxiv - Systems Biology 2019Quote: ... product quality was verified by DNA gel electrophoresis and sample DNA concentrations determined using Quant-iT PicoGreen dsDNA Assay Kit (Thermo Fisher Scientific). The libraries were multiplexed together and sequenced using the paired-end with 250-bp paired-end reads approach on the MiSeq Illumina sequencing machine at the BioMicro Center (Massachusetts Institute of Technology ...
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bioRxiv - Immunology 2019Quote: Boiled samples were subjected to SDS-PAGE under reducing conditions on NuPAGE 4-12% Bis-Tris protein gels (Thermo Fisher Scientific # NP0322) and stained using InstantBlue protein stain (Expedeon).
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bioRxiv - Genomics 2019Quote: ... The integrity and quantity of DNA was confirmed by 1.5% agarose gel electrophoresis and a nanodrop spectrophotometer (Thermo Fisher Scientific, Waltham, USA)
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bioRxiv - Genetics 2021Quote: ... 2 μl of this DNA was loaded on a 1% agarose gel to check for good quality and then quantified using a Qubit fluorometer (Thermo Fisher Scientific). 0.5-1 μg of genomic DNA from each genotype and treatment were used for bisulfite conversion ...
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bioRxiv - Cell Biology 2021Quote: ... per sample were reduced with DTT and denatured at 70°C for 10 min prior to loading onto Novex NuPAGE 10% Bis-Tris Protein gels (Invitrogen, Cat. # NP0315BOX). The gels were stained overnight with Novex Colloidal Blue Staining kit (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... were boiled for 5 min and subjected to SDS-PAGE using NuPAGE™ 4-12% Bis-Tris Gels (Invitrogen; Thermo Fisher Scientific) at 120V for 80 min and transferred to 0.45 μm PVDF membranes (Sigma ...
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bioRxiv - Cell Biology 2020Quote: Protein samples were boiled at 70°C for 10 min and loaded on a 4-12% Bis-Tris gel (NuPAGE, Thermo Scientific, #NP0321), running at 150-180 V for 60-120 min in 1x MOPS ...
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bioRxiv - Biochemistry 2021Quote: ... 10 μg purified carboxysomal proteins or 100 μg whole cell fractions were loaded per-well on 15% polyacrylamide gels and stained with Coomassie Brilliant Blue G-250 (ThermoFisher Scientific, UK).
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bioRxiv - Molecular Biology 2021Quote: ... followed by separation on 20% 7 M urea SDS-PAGE gel and immunoblot assay with anti-Biotin antibody (Thermo Fisher Scientific, 692033).
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bioRxiv - Cell Biology 2021Quote: ... and an equal protein mass of 30ug in 10μL was subjected to NuPAGE™ 4-12 % Bis-Tris Midi Protein Gels (Invitrogen™, NP0335BOX) in Xcell4 Surelock tank (Life Technology SAS ...
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bioRxiv - Synthetic Biology 2021Quote: ... Methylation was analyzed by DNA gel electrophoresis of 600 ng of DpnI- or MboI-digested plasmid DNA and GeneRuler 1 kb DNA Ladder (Thermo Fisher SM0313). All annotated plasmid sequences for the transactivator- and reporter-expressing plasmids are available in the Haynes Lab Benchling database (https://benchling.com/hayneslab/f_/mrI2t4mM-collection-dal-e/).
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bioRxiv - Cell Biology 2020Quote: ... 5 μg protein from each sample was fractionated on a 4-12% Bis-Tris SDS-PAGE gel (Life Technologies/ Thermo Fisher Scientific), then electrotransferred to nitrocellulose ...
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bioRxiv - Cell Biology 2020Quote: ... 5 μg protein from each sample was fractionated on a 4-12% Bis-Tris SDS-PAGE gel (Life Technologies/ Thermo Fisher Scientific), then electrotransferred to nitrocellulose ...
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bioRxiv - Bioengineering 2021Quote: Samples from time course experiments and the truncation screening were prepared for sodium dodecyl-sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) by adding 12 µL of NuPAGE LDS Sample Buffer (Thermo Fisher Scientific) and 3 µL of tris(2-carboxyethyl)phosphine (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... were boiled for 5 min and subjected to SDS-PAGE using NuPAGE™ 4-12% Bis-Tris Gels (Invitrogen; Thermo Fisher Scientific) at 120V for 80 min and transferred to 0.45 μm PVDF membranes (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... were boiled for 5 min and subjected to SDS-PAGE using NuPAGE™ 4-12% Bis-Tris Gels (Invitrogen; Thermo Fisher Scientific) at 120V for 80 min and transferred to 0.45 μm PVDF membranes (Sigma ...
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bioRxiv - Biochemistry 2021Quote: ... Samples were stained for DNA using either SYBR Green I Nucleic acid gel stain Ex504/Em523 (10,000 X concentrate in DMSO – subsequently diluted to 1:1000) (Invitrogen, Thermo Fisher, UK) or SYTO 9 Green Fluorescent Nucleic acid stain Ex485/Em498 ...
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bioRxiv - Genomics 2020Quote: ... followed by a second size selection on a UltraPure™ Low Melting Point Agarose (LMP-Agarose gel; Thermo Fisher Scientific, Massachusetts, USA), removing fragments smaller than 300bp and those larger than 400bp ...
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bioRxiv - Cancer Biology 2021Quote: ... In-vitro transcribed products and chemically synthesized 5’-tRFCys (Integrated DNA Technologies) were purified on a 10% TBE-Urea PAGE gel (Thermo Fisher Scientific) before 5’ radiolabeled using T4 PNK (New England Biolabs) ...
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bioRxiv - Microbiology 2021Quote: ... 10min and the supernatant was boiled for 5min in 4x loading dye with 4x DTT prior to loading on 4-12% Bis-Tris polyacrylamide gels (Thermo Fisher Scientific). After transfer to nitrocellulose ...
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bioRxiv - Genetics 2021Quote: ... SDS-Page separation was completed by running between 3 and 10μg of total protein on a NuPAGE 3%–8% Tris-acetate gel (Thermo Fisher Scientific Scientific). Next ...
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bioRxiv - Plant Biology 2020Quote: A total of 85 μg of protein per sample was subjected to one-dimensional SDS-PAGE using an 8-16% precise protein gradient gel (Thermo Fisher Scientific). Proteins were separated for 1 cm in the resolving gel and the entire lane was excised and cut into equal pieces ...
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bioRxiv - Microbiology 2021Quote: ... The fragments were purified (NucleoSpin Gel and PCR Clean-up Columns, Macherey Nagel) and ligated with T4 DNA ligase (EL0014, Thermo Fisher Scientific). E ...
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bioRxiv - Microbiology 2020Quote: ... The amplified band was purified using a Qiagen gel purification kit and TA cloning was performed using a TOPO™ TA cloning™ kit (Invitrogen). 12 clones were randomly picked for Sanger sequence analysis by the Advanced Genomics Core at the University of Michigan.
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bioRxiv - Microbiology 2020Quote: ... PCR products were verified visually by running a high-throughput Invitrogen 96-well E-gel (Thermo Fisher Scientific Corp., Carlsbad, CA, USA). Any samples with failed PCRs (or spurious bands ...
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bioRxiv - Molecular Biology 2020Quote: ... and protease contaminant activities (not shown) and analysed by SDS-PAGE through Novex Value 4-20% Tris-Glycine Mini Gel (Thermo Fisher Scientific) followed by Coomassie staining.
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bioRxiv - Molecular Biology 2020Quote: The immune precipitated samples using HA antibodies were resolved by SDS-PAGE and the gel was silver stained using Pierce Silver Stain Kit (Thermo Scientific, USA). The distinct protein bands were excised and were subjected to in-gel trypsin digestion using In-Gel Tryptic Digestion Kit (Thermo Scientific ...
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bioRxiv - Genomics 2021Quote: ... Proteins (20 μg) were resolved by SDS-PAGE and transferred to a membrane using an iBlot2 Gel Transfer Device (Thermo Fisher Scientific). Membranes were incubated with specific primary antibodies against ...
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bioRxiv - Cell Biology 2021Quote: ... at 95°C for 10 min and run under reducing conditions on a 3-8% Tris-Acetate precast gel for 135 min at 100 V in Tris-Acetate running buffer supplemented with antioxidant (Thermo Fisher Scientific). Transfer of protein bands onto a nitrocellulose membrane (GE Healthcare ...
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bioRxiv - Genomics 2021Quote: ... DNA samples were run on 1% agarose gel electrophoresis for quality control and quantified using the NanoDrop spectrophotometer (Thermo Fisher Scientific Inc.). For each sample ...
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bioRxiv - Genomics 2021Quote: Cells were lysed in RIPA buffer and 20-30 μg of total proteins were loaded into NuPAGE 4%- 12% Bis-Tris Gels (Invitrogen, Cat# NP0336BOX). Subsequently ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The PCR products were subjected to agarose gel electrophoresis and the bands corresponding to the host cDNA and the parasitic cDNA were separately extracted by using E-gel CloneWell (Thermo Fisher Scientific). The host and the parasite-β and -γ were sequenced using PacBio RS II with C4/P6 chemistry (Pacific Biosciences) ...
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bioRxiv - Genetics 2020Quote: ... incubated for 5 min at 95 °C and 80 μg of protein was separated on a 10% SDS-PAGE gel (alongside PageRuller™ Plus Prestained protein Ladder, Thermo Scientific). Samples were transferred onto a nitrocellulose membrane (Amersham Protan 0.45 μm ...
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bioRxiv - Microbiology 2021Quote: ... Equal amounts of the cell lysate protein and equal volume of the virus pellet protein were resolved by SDS-PAGE (NuPAGE 4-12% Bis-Tris gel; Invitrogen/Thermo Scientific) and transferred onto nitrocellulose membrane by semidry transfer ...
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bioRxiv - Molecular Biology 2021Quote: ... After incubation for 1 h at 37 °C the DNA products were analyzed by electrophoresis on a denaturing polyacrylamide gel (10%) and detected by chemiluminescent detection (Thermo Fisher Scientific). To semi-quantify the level of dCTP incorporation ...
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bioRxiv - Biochemistry 2021Quote: PCR products were visualized by agarose gel electrophoresis of the total 20 µL PCR reaction in 1.3 % agarose 1xTAE electrophoresis buffer (Thermo scientific, Vilnius, Lithuania). The agarose was supplemented with peqGreen DNA/RNA dye (peqlab brand ...
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bioRxiv - Biochemistry 2021Quote: ... Usually 5 µg of total protein or the total protein of 1 to 2 × 106 autophagosomes were subjected to 4 – 12 % NuPage Bis-Tris gels (Thermo Scientific, MP0335) and transferred onto a nitrocellulose membrane using the Trans-Blot Turbo RTA mini nitrocellulose transfer kit (BioRad ...
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bioRxiv - Cell Biology 2022Quote: ... Then the gel was stained with Coomassie blue or silver staining and bands cut-off were analyzed by mass spectrometry or proteins from the gel were then transferred into PVDF or nitrocelluse membranes using iBlot system (Thermo Fisher Scientific). For SDS-PAGE analysis of steady state level of proteins ...
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bioRxiv - Cell Biology 2022Quote: ... pH 7.4) for different time intervals up to 60 mins and separated using NativePAGE™ 3-12 % Bis-Tris Gels (Thermo Fisher Scientific) to separate monomeric and dimeric ATP synthase complexes (Schagger & von Jagow ...
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bioRxiv - Neuroscience 2022Quote: ... 30 to 60 µg of protein per lane were loaded and separated on 10% Bis-tris BOLT SDS-Page gels (catalog number NW00105BOX, Thermo Fisher Scientific) at 100V for 1.5 hours in MOPS buffer ...
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bioRxiv - Genetics 2022Quote: ... and boiled at 95°C for 10 minutes before the samples were loaded in the NuPAGE 4%–12% Bis-Tris Gels (Invitrogen, #NP0335BOX & #NP0336BOX) for further resolution and western blot analysis.
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bioRxiv - Biochemistry 2022Quote: ... PCR reaction mixture was loaded on 1% agarose gel (140 V for 2 h) and post-stained with SYBR gold (Thermo Fisher Scientific). The product bend was cut and DNA was extracted from the gel by Monarch DNA Gel extraction Kit (NEB) ...
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bioRxiv - Genetics 2022Quote: ... and boiled at 95°C for 10 minutes before loading the samples in NuPAGE 4%–12% Bis-Tris Gels (Invitrogen, #NP0335BOX & #NP0336BOX) for further resolution and western blot analysis ...
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bioRxiv - Cell Biology 2022Quote: ... Equal amount of protein from both PNS and LM (10 μg/lane) was resolved by SDS-PAGE (Invitrogen NuPage gel 4–12%).
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bioRxiv - Developmental Biology 2022Quote: ... 10μl of the concentrated lysate was resolved on a 4-12% NuPAGE Bis-Tris gel and transferred onto a PVDF membrane using the iBlot system (Invitrogen, Waltham MA). Blots were subsequently incubated overnight a 4°C with rabbit anti-Npr1 (Novus Biologicals ...
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bioRxiv - Genomics 2022Quote: ... The quality and concentration of the extracted genomic DNA was assessed using 1% agarose gel electrophoresis and a Qubit fluorimeter (Invitrogen, Carlsbad, CA). This high-quality DNA was used for subsequent Nanopore and Illumina sequencing ...
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bioRxiv - Microbiology 2022Quote: ... 10 μg purified carboxysomal proteins or 100 μg whole cell fractions were loaded per-well on 15% polyacrylamide gels and stained with Coomassie Brilliant Blue G-250 (ThermoFisher Scientific, UK).
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bioRxiv - Microbiology 2022Quote: ... proteins were separated on Bolt 4-12% Bis-Tris gel in 1 × Bolt MOPS SDS running buffer (Thermo Fisher Scientific catalog # B0001). Separated proteins were electro-transferred to nitrocellulose membrane using Trans-Blot Turbo Transfer System (Bio-Rad) ...