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Citations for Thermo Fisher :
8801 - 8850 of 10000+ citations for rno mir 134 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... (Applied Biosystems™ ProFlex™ PCR System). The PCR reaction was finally purified with 1.4X SPRI beads (Beckman Coulter ...
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bioRxiv - Physiology 2024Quote: ... with SYBR Green PCR Master Mix (Invitrogen). Actin was used to normalize the results from gene-specific reactions.
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bioRxiv - Cancer Biology 2024Quote: ... Platinum Superfi II PCR MM (Invitrogen #12368010) was used for all amplification reactions following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ProFlex PCR System (ThermoFisher, cat. no. 4484073)
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bioRxiv - Immunology 2024Quote: ... StepOneTM Real-Time PCR System (Applied Biosystems) was used for qPCR analysis.
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bioRxiv - Cell Biology 2024Quote: ... in a StepOnePlus PCR machine (Applied Biosystems). Primers with the following sequences were purchased from Life Technologies ...
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bioRxiv - Cell Biology 2024Quote: ... in 384 well PCR plates (Thermofisher, AB1384) using the QuantStudio™ 12K Flex Real-time PCR System instrument (Thermofisher) ...
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bioRxiv - Developmental Biology 2024Quote: ... into 96 well PCR plates (Thermo Scientific) containing 2μl of lysis buffer (0.1% Triton X-100 ...
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bioRxiv - Immunology 2023Quote: ... RT-qPCR was performed using PCR master mix (Powerup™ SYBR™ Green Master Mix Catalog: A25741) and real-time PCR system (Applied biosystems™ 7500 Fast Real-Time PCR system). CT values were determined after normalizing to housekeeping gene GAPDH.
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bioRxiv - Immunology 2020Quote: Cytokine concentrations for IL-17A and IFNγ from the supernatants of cultured cells and ex vivo colon organ cultures were measured by ELISA (Thermo Fisher Ready-Set-Go). Samples and standards were measured in duplicate ...
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bioRxiv - Cell Biology 2020Quote: ... In a second set of experiments Human HK-2 cells were cultured in DMEM and Keratinocyte-SFM (1X) medium (Life Technologies Green Island NY). When the cells reached 70% confluence ...
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bioRxiv - Immunology 2021Quote: ... dissolved in PBS was used at a final concentration of 4% formaldehyde for 10 minutes in order to fix the samples before permeabilization with the FoxP3/Transcription Factor Staining Buffer Set (ThermoFisher Scientific #00-5523-00). The samples were incubated with SCN-EDTA coupled palladium based barcoding reagents for 15 minutes and then combined into a single sample ...
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bioRxiv - Microbiology 2020Quote: ... A total of 100 μg protein from each condition was subjected to in-solution trypsin digestion before labelling the resultant tryptic peptides using the TMT-6plex Isobaric Label Reagent Set (Thermo Scientific, Rockford, IL, USA) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... Samples that were stained for intracellular markers were fixed and permeabilized using the eBioscience™ FoxP3/Transcription Factor Staining Buffer Set (Thermofisher, #00-5523-00) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... Larvae were then transferred to imaging slides and arrayed as needed and tails were cut to facilitate mounting using SlowFade™ Glass Soft-set Antifade Mountant with DAPI (Invitrogen, Catalogue number S36920).
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bioRxiv - Genetics 2022Quote: ... Whole mitochondrial genome of all samples was also PCR amplified using a set of 24 markers and sequencing was done using Sanger sequencing method in ABI 3730 Automated DNA analyzer (Applied Biosystems, Foster city, USA). All mtDNA sequences were assembled with the revised Cambridge reference sequence (rCRS ...
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bioRxiv - Immunology 2022Quote: ... Cells were then fixed for 30 min at room temperature using the fixation/permeabilization buffer supplied with the eBioscience Foxp3/Transcription Factor Staining buffer set (ThermoFisher Scientific, 00-5523-00).
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bioRxiv - Immunology 2020Quote: ... Cells were fixed and permeabilized for intracellular/nuclear staining using the eBioscience Foxp3/ Transcription Factor Fixation/Permeabilization Staining buffer set (ThermoFisher Scientific, #00-5523-00). Samples were stained for 1hr at 4°C (extracellular ...
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bioRxiv - Microbiology 2020Quote: ... RNA was isolated as described by the manufacturer with an on-column DNA digestion using the PureLink™ DNase Set (Invitrogen, Thermo Fisher Scientific). RNA was eluted in nuclease-free H2O and stored at −20° C for short-term storage or −80° C for long-term storage.
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bioRxiv - Microbiology 2022Quote: ... RNA was isolated as described by the manufacturer with an on-column DNA digestion using the PureLink™ DNase Set (Invitrogen, Thermo Fisher Scientific). RNA was eluted in nuclease-free H2O and stored at -20° C for short-term storage or -80° C for long-term storage.
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bioRxiv - Microbiology 2022Quote: TNF-α was quantified in cell culture supernatants of infected cells by Ready-SET-Go! ELISA kits (Thermo Fisher Scientific, Cat. 88-7324-77) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... was set up with an Acclaim Pepmap RSLC analytical column (C18, 100 Å, 75 μm × 50 cm, Thermo Fisher Scientific, San Jose, CA, USA) and Acclaim Pepmap nano-trap column (75 μm × 2 cm ...
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bioRxiv - Genetics 2022Quote: The data files in each set were merged for identification and relative quantification using Proteome Discoverer version 2.4 (Thermo Fisher Scientific, Waltham, MA, USA). The Uniprot Equus caballus proteome database (July 2021 ...
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bioRxiv - Genomics 2024Quote: ... Cells were then washed twice in FACS buffer and afterwards fixed and permeabilized with the Fixation/Permeabilization solution from the eBioscience™ FoxP3 / Transcription Factor Staining Buffer Set (Thermofisher, 00-5523-00) according to the manufacturer’s instructions for 30min at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... followed by a peptide clean-up on SPE spin columns before TMT labeling with the TMTpro™ 16plex Label Reagent Set (Thermo Fisher, Cat# A44520). Peptides from each sample were labeled with a distinct TMTpro™ isobaric tag before mixing ...
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bioRxiv - Neuroscience 2023Quote: ... qPCR reactions were set up using TaqMan™ Gene Expression Master Mix on MicroAmp™ Fast Optical 96-Well Reaction Plates (Thermo Fisher Scientific) and run on StepOnePlus Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Immunology 2023Quote: ... all cells were fixed either in 4% paraformaldehyde (PFA) for 10 minutes at room temperature or using eBioscience™ Foxp3/Transcription Factor Staining Set (Invitrogen, #00-5523-00) for 30 minutes at 4°C ...
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bioRxiv - Genomics 2023Quote: ... dissolved in PBS was used at a final concentration of 4% for 10 minutes to fix the samples before permeabilization with the FoxP3/Transcription Factor Staining Buffer Set (ThermoFisher Scientific #00-5523-00). The samples were incubated with SCN-EDTA coupled palladium barcoding reagents for 15 minutes followed by incubation with Heparin (Sigma-Aldrich #H3149-100KU ...
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bioRxiv - Cancer Biology 2023Quote: DNA PCR reactions were set up using the following volumes: 2.4 µL 5X dPCR QuantStudio™ Absolute Q™ Master Mix (PN A52490, ThermoFisher Scientific, Waltham, MA), 2.9 µL oligonucleotide primer-probe mix (Table S3) ...
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bioRxiv - Plant Biology 2023Quote: ... was set up with an Acclaim Pepmap RSLC analytical column (C18, 100 Å, 75 μm × 50 cm, Thermo Fisher Scientific, San Jose, CA, USA) and Acclaim Pepmap nano-trap column (75 μm× 2 cm ...
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bioRxiv - Plant Biology 2024Quote: ... The cDNA probe preparation and its hybridization to the probe sets on the Affymetrix® Soybean GeneChip® were performed according to Affymetrix® guidelines (Affymetrix®). The experiment was run using probe generated from each of the G ...
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bioRxiv - Immunology 2021Quote: ... The qRT-PCR was performed with SYBR Green Real-Time PCR Master Mixes (Applied Biosystems, Foster City, CA, USA) using the Applied Biosystems ViiA 7 Real-Time PCR instrument ...
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bioRxiv - Developmental Biology 2021Quote: ... PCR products were subcloned into Blunt-TOPO vector using Zero Blunt TOPO PCR Cloning Kit (Invitrogen, cat. K2800-20SC) and sequenced using Sanger sequencing.
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bioRxiv - Evolutionary Biology 2021Quote: ... The final PCR products of the inserts and vectors were purified by PureLink PCR purification kit (Thermo Fisher Scientific), digested by DpnI and BsmBI (New England Biolabs) ...
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bioRxiv - Neuroscience 2019Quote: ... PCR products for individual genes were amplified from a hypothalamic cDNA library and cloned into pCR 2.1-TOPO or pCR II-TOPO (Invitrogen) for all probes except Tac1 ...
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bioRxiv - Genetics 2019Quote: Genomic DNA extraction and PCR for deletion detection was performed using Phire Animal Tissue Direct PCR Kit (Thermo Scientific) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2019Quote: ... Real-time PCR was performed by using Power SYBR Green PCR master mix (Applied Biosystems, Foster City, CA, USA). For quantification of gene expression ...
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bioRxiv - Molecular Biology 2019Quote: ... cDNA levels were measured using real-time quantitative PCR with the Power SYBR Green PCR master mix (ThermoFisher Scientific) on a ViiA-7 real-time thermal cycler (Applied Biosystems) ...
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bioRxiv - Cancer Biology 2019Quote: ... was used for real time PCR and was performed and analyzed using Viia7 Real Time PCR System (Life Technologies) and software.
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bioRxiv - Developmental Biology 2019Quote: ... q-PCR analysis was performed using the SYBR Green PCR Master Mix and an ABI 7900HT instrument (Applied Biosystems). Gapdh was measured as an internal control to normalize sample differences ...
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bioRxiv - Microbiology 2019Quote: ... was generated by RT-PCR amplification of the 3’ UTR region from ZIKV-infected cells and the resulting PCR product cloned into pCR-TOPO2.1 (Invitrogen). The actin and ZIKV probes were randomly labeled with α32P dATP (Perkin Elmer ...
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bioRxiv - Plant Biology 2019Quote: ... Quantitative real-time PCR (qPCR) was performed on Step One Real-Time PCR System (Life Technologies Corporation, CA, USA), and AceQ qPCR SYBR Green Master Mix (Vazyme ...
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bioRxiv - Cancer Biology 2019Quote: Quantitative real-time PCR (qPCR) reactions were performed using TaqMan Universal PCR Master Mix (Thermo Fisher Scientific, Waltham, MA) and analyzed by the Quant Studio7 Real-timePCR system (Life Technologies ...
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bioRxiv - Microbiology 2019Quote: ... Quantitative PCR were performed on 2µL of cDNA using SYBR Green PCR Master Mix (Thermo Fisher; catalog no. 4309155) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... encompassing the 5’ and 3’ flanking sequences of the CNBG_3981 locus were PCR amplified and gel purified using the PureLink Quick Gel Extraction & PCR Purification Combo Kit (Invitrogen). Both fragments were mixed (100 ng of each ...
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bioRxiv - Genetics 2020Quote: ... PCR amplification was performed using DreamTaq Green PCR master mix following the manufacturer’s protocol (Thermo Fisher Scientific, Waltham, MA) using the BioRad T100™ thermal cycler ...
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Efficient breeding of industrial brewing yeast strains using CRISPR/Cas9-aided mating-type switchingbioRxiv - Microbiology 2021Quote: ... PCR reactions were carried out with Phusion High-Fidelity PCR Master Mix with HF Buffer (Thermo Scientific, Vantaa, Finland) and primer concentrations of 0.5 μM ...
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bioRxiv - Cancer Biology 2020Quote: ... Gene expression was assessed via quantitative real-time PCR (qRT-PCR) using Power SYBR Green Master Mix (Applied Biosystems) and monitored using a StepOne® Real-Time PCR system (Applied Biosystems) ...
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bioRxiv - Biochemistry 2021Quote: ... PCR reactions were analyzed for purity on an agarose gel and purified using GeneJET PCR purification kit (Thermo Fisher).
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bioRxiv - Biochemistry 2020Quote: Quantification by qRT-PCR was done on the StepOnePlus 96-well real-time PCR system (Applied Biosystems, catalog # 4376600) using the PowerUp SYBR green qPCR kit (Applied Biosystems ...